FastQCFastQC Report
Mon 15 Aug 2016
SRR2921434_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921434_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5428266
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81340.14984527287351063No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72420.13341276938160362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG184500.025.9281441
TACATGG188900.024.4659752
GTATCAA202500.024.0704771
ACATGGG195850.022.652753
TCAACGC233900.020.3896224
TATCAAC236700.020.2476462
CAACGCA236800.020.0272395
ATCAACG240600.019.8412083
CATGGGG153450.019.7806074
AACGCAG246850.019.2319876
GAGTACA144250.019.0917641
AGAGTAC179200.019.0883910-11
TAGATCG4550.018.7801235
GTACCTG51150.018.5370861
AGTACAT140450.018.4989552
GTATATA13900.017.4819321
ACGCAGA275050.017.1924217
GGTATCA153450.016.7672161
GAGTACT86600.016.58031312-13
CGCAGAG284300.016.5328488
Produced by FastQC (version 0.11.5)