##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921434_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5428266
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.952901534302114	33.0	31.0	34.0	30.0	34.0
2	32.07159763357212	34.0	31.0	34.0	30.0	34.0
3	32.196757307029536	34.0	31.0	34.0	30.0	34.0
4	35.726108668956165	37.0	35.0	37.0	35.0	37.0
5	35.532996909141886	37.0	35.0	37.0	33.0	37.0
6	35.571348935369045	37.0	35.0	37.0	33.0	37.0
7	35.508206856480506	37.0	35.0	37.0	33.0	37.0
8	35.463423126280105	37.0	35.0	37.0	33.0	37.0
9	37.093638189432866	39.0	37.0	39.0	34.0	39.0
10-11	37.006886453243084	39.0	37.0	39.0	33.5	39.0
12-13	36.89248399028345	39.0	37.0	39.0	33.0	39.0
14-15	38.19350359764978	40.0	38.0	41.0	33.0	41.0
16-17	38.10292476455649	40.0	38.0	41.0	33.0	41.0
18-19	38.00712649306426	40.0	38.0	41.0	33.0	41.0
20-21	37.899165313564225	40.0	38.0	41.0	33.0	41.0
22-23	37.86763047352506	40.0	38.0	41.0	33.0	41.0
24-25	37.91749123937552	40.0	38.0	41.0	33.0	41.0
26-27	37.80802433042153	40.0	38.0	41.0	33.0	41.0
28-29	37.68019557258248	40.0	38.0	41.0	32.5	41.0
30-31	37.544516149355985	40.0	38.0	41.0	32.0	41.0
32-33	37.38664860933491	40.0	37.0	41.0	32.0	41.0
34-35	37.242195113504025	40.0	37.0	41.0	31.5	41.0
36-37	37.14678352166235	40.0	37.0	41.0	31.0	41.0
38-39	37.114495678730556	40.0	37.0	41.0	31.0	41.0
40-41	36.956544041872675	40.0	37.0	41.0	31.0	41.0
42-43	36.72735584438935	40.0	36.0	41.0	30.5	41.0
44-45	36.52361140739971	39.0	36.0	41.0	30.0	41.0
46-47	36.247898960736265	39.0	35.5	40.5	30.0	41.0
48-49	35.91373294897487	39.0	35.0	40.0	29.0	41.0
50-51	35.642820653962055	39.0	35.0	40.0	28.0	41.0
52-53	35.340646165829014	38.0	35.0	40.0	28.0	41.0
54-55	35.02176090854796	38.0	34.0	40.0	27.0	41.0
56-57	34.6383712588882	38.0	34.0	40.0	26.0	41.0
58-59	34.25150315036146	37.0	33.5	40.0	26.0	41.0
60-61	33.825038235045966	37.0	33.0	39.5	25.0	41.0
62-63	33.390136095025554	36.0	32.5	39.0	23.5	40.0
64-65	32.8749108831439	35.5	32.0	39.0	22.5	40.0
66-67	32.34746445734236	35.0	31.5	38.0	21.5	40.0
68-69	31.84073965793128	35.0	31.0	37.5	20.0	39.5
70-71	31.28495232179116	34.5	30.5	37.0	18.0	39.0
72-73	30.73817587421103	34.0	30.0	36.0	16.0	39.0
74-75	30.220438165705218	34.0	29.0	36.0	12.5	38.0
76-77	28.921447014571505	32.5	28.0	34.5	11.0	36.0
78-79	29.859526320191385	34.0	29.5	35.0	11.0	37.0
80-81	29.889233873211076	34.0	30.0	35.0	9.0	36.5
82-83	29.55770710204695	34.0	29.5	35.0	7.0	36.0
84-85	29.07318884151956	34.0	29.0	35.0	2.0	36.0
86-87	28.563840552397394	33.5	29.0	35.0	2.0	35.0
88-89	28.020918282191772	33.0	27.5	35.0	2.0	35.0
90-91	27.549070366116915	33.0	26.5	35.0	2.0	35.0
92-93	27.11978862126506	33.0	26.0	34.0	2.0	35.0
94-95	26.63071770985431	32.0	25.0	34.0	2.0	35.0
96-97	25.866416273631394	32.0	24.5	34.0	2.0	35.0
98-99	24.85233866579125	32.0	18.5	34.0	2.0	35.0
100-101	21.22479167380523	27.5	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35620.0
3	18165.0
4	10722.0
5	7927.0
6	7058.0
7	7155.0
8	8431.0
9	10067.0
10	11897.0
11	14139.0
12	16494.0
13	19500.0
14	22083.0
15	24703.0
16	28562.0
17	33471.0
18	34322.0
19	30335.0
20	30019.0
21	31537.0
22	34070.0
23	37515.0
24	43006.0
25	50616.0
26	59611.0
27	72121.0
28	87998.0
29	107790.0
30	132708.0
31	165652.0
32	213713.0
33	287928.0
34	410657.0
35	624234.0
36	975050.0
37	1199173.0
38	509414.0
39	14803.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.7188521761823	17.75069025379568	14.307451672993338	24.223005897028678
2	15.934517578910098	19.16787055019043	38.50883504971937	26.388776821180098
3	20.37378993591462	22.322973001603746	27.813902760083753	29.48933430239788
4	14.228634494169947	15.278510276166385	34.75114093088752	35.741714298776145
5	15.829603921855433	35.77062723932791	32.52077512316591	15.878993715650743
6	35.006107202886334	33.30552863159206	16.337514300219254	15.350849865302349
7	31.18564911706275	30.336744732095315	19.1822045334879	19.295401617354035
8	27.871168838066446	33.284868951611045	18.838296967527572	20.005665242794933
9	26.82177832604058	16.700097175278756	19.03075496156321	37.44736953711745
10-11	26.669974811812363	25.00375024536793	25.82737512236126	22.498899820458444
12-13	26.981522411387644	22.045676914742057	26.492070259240176	24.480730414630123
14-15	23.971958809408903	23.57771828074888	24.29426954390759	28.156053365934625
16-17	24.22587987870497	26.750522250675346	24.85676403889336	24.166833831726322
18-19	24.25092073021499	26.10014913028222	26.28197307546007	23.36695706404272
20-21	24.885257640752993	25.09817619215132	26.222749898972182	23.793816268123507
22-23	24.62786206520324	24.795327015562272	26.172809858767508	24.404001060466985
24-25	24.452758769547454	25.022383717314174	26.216338616323686	24.30851889681468
26-27	24.41032253642246	25.129005908705555	26.309291355180697	24.15138019969129
28-29	24.319487091795445	25.444606311922602	26.103495600464054	24.1324109958179
30-31	24.521376171372296	25.150022885588548	25.96043708572548	24.368163857313675
32-33	24.370924737204994	25.1953984448081	25.956155618675382	24.477521199311525
34-35	24.631649558335045	25.154060654442162	26.032278006604315	24.182011780618478
36-37	24.566393689233905	25.209753638619247	26.0235456162922	24.20030705585465
38-39	24.510591592337725	25.285383253419297	26.003914032783133	24.200111121459848
40-41	24.623692844990988	25.231203344518455	25.86011550447574	24.284988306014814
42-43	24.581830340858428	25.217434958346075	25.75581331083297	24.44492138996253
44-45	24.587688219377828	25.0947931326833	25.838260193296094	24.47925845464278
46-47	24.691525002724855	25.24583793118666	25.792319498881362	24.270317567207126
48-49	24.671960036193067	25.337329566645785	25.64475692881401	24.34595346834714
50-51	24.490246164928568	25.476057344830792	25.65837580706226	24.375320683178376
52-53	24.711251875760908	25.502684579893575	25.4497197515793	24.336343792766222
54-55	24.63467155143771	25.229500998198883	25.572820987517286	24.56300646284612
56-57	24.715232027209723	25.321102320267492	25.532209070294826	24.43145658222796
58-59	24.806326150962068	25.23575042767416	25.55802128072705	24.399902140636726
60-61	24.791864868668988	25.2233342004479	25.695011978708777	24.289788952174334
62-63	24.489354471309984	25.346373170527908	25.677027545042353	24.48724481311975
64-65	24.56157578473089	25.328997790652945	25.640953836584963	24.468472588031204
66-67	24.636011290254658	25.36919042431155	25.482596095111116	24.512202190322675
68-69	24.589633587822846	25.34851213315325	25.483732536604258	24.578121742419647
70-71	24.688679195745493	25.18364373828721	25.572289797598756	24.55538726836854
72-73	24.745849574349695	25.184072530506295	25.516213466178648	24.55386442896536
74-75	24.499417237440976	25.5207700225502	25.430666922375032	24.549145817633793
76-77	24.674544058603555	25.213672646953565	25.46827706068195	24.643506233760927
78-79	24.633810536647708	25.217171354969686	25.594002993636078	24.555015114746524
80-81	24.61421228356382	25.37206314133825	25.460215548160637	24.553509026937302
82-83	24.689751415074827	25.334116872884806	25.454393510995217	24.521738201045157
84-85	24.627359386215254	25.389744341420805	25.442948772930762	24.539947499433175
86-87	24.623146858602677	25.3807817209281	25.48446486568039	24.511606554788838
88-89	24.551296138297722	25.237137544915768	25.432381801194246	24.779184515592267
90-91	24.653565724653696	25.196538110629497	25.414677059172273	24.735219105544534
92-93	24.669343455106656	25.387982725747698	25.49004645904277	24.452627360102877
94-95	24.62909121659776	25.280946280981837	25.45476385810354	24.63519864431686
96-97	24.50785513019235	25.308866635549943	25.584071848862997	24.599206385394705
98-99	24.610409840943415	25.34366823756179	25.392197160818476	24.65372476067632
100-101	25.040230124881273	24.724247814805736	25.53202837484666	24.703493685466334
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	377.0
1	318.0
2	451.0
3	692.5
4	577.0
5	502.0
6	1073.5
7	2738.5
8	4078.0
9	3780.5
10	2591.5
11	1658.0
12	1448.5
13	1498.5
14	1611.0
15	1690.5
16	1759.5
17	1880.5
18	2131.5
19	2249.0
20	2261.5
21	2470.0
22	2731.0
23	3108.0
24	3514.0
25	4144.5
26	5114.0
27	6476.0
28	8562.0
29	11389.5
30	15398.0
31	20428.0
32	26852.0
33	34521.5
34	43089.0
35	52999.0
36	64993.5
37	77495.0
38	90725.0
39	104721.5
40	119089.0
41	134461.5
42	149898.5
43	167215.0
44	185529.5
45	202189.0
46	216023.0
47	233899.5
48	257274.5
49	263942.5
50	255018.5
51	249890.5
52	249058.5
53	248082.0
54	244040.5
55	235244.5
56	219239.5
57	199221.5
58	180704.0
59	162773.0
60	141574.0
61	118979.5
62	96766.5
63	73383.0
64	53558.0
65	40153.0
66	30447.5
67	23446.0
68	17630.5
69	12785.5
70	9515.5
71	7106.0
72	5107.5
73	3702.0
74	2664.0
75	1829.0
76	1236.0
77	752.0
78	443.5
79	229.5
80	113.5
81	58.0
82	36.0
83	20.0
84	10.5
85	5.5
86	1.5
87	1.0
88	1.0
89	1.5
90	1.5
91	1.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3237682162222706
2	0.0
3	0.006466153279887168
4	0.06501155249208494
5	3.500196932132655E-4
6	0.0649194420465025
7	0.07549372119936643
8	0.10550330437012483
9	0.09351052435529136
10-11	0.0974067962034285
12-13	0.09697387710919105
14-15	0.06506681875943442
16-17	0.03670601256460166
18-19	0.039386426531050615
20-21	0.028425283506740458
22-23	0.04337480882477019
24-25	0.03479011529648695
26-27	0.021903863959503826
28-29	0.024501378524928587
30-31	0.0032699208181765594
32-33	0.00586743538360132
34-35	0.008335995325210666
36-37	0.010850610489611232
38-39	0.016054850665018994
40-41	0.020577473543116714
42-43	0.003794950357996458
44-45	0.014544239357467008
46-47	0.025192206866796872
48-49	0.0028462127684973433
50-51	0.007387257735711551
52-53	0.002496193075284078
54-55	0.004412090343398794
56-57	0.0038041614025547017
58-59	0.0012527020599211608
60-61	0.0055634709131792736
62-63	0.015594298437106803
64-65	0.007700433250691841
66-67	0.039985144427336465
68-69	0.0629851226892713
70-71	0.012996783871682042
72-73	0.033242659810701986
74-75	0.05184896981835452
76-77	0.3155059092535259
78-79	0.07540161075378399
80-81	0.03442167351415719
82-83	0.02965035243298689
84-85	0.020015599825063842
86-87	0.1189882736033938
88-89	0.5366630890969603
90-91	0.1322705998563814
92-93	0.0503752026890355
94-95	0.00827151801330296
96-97	0.005830591205368344
98-99	0.010592701241980404
100-101	0.007930709364647936
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5428266.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.006357202695035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.59233867104585	32.448619544077644
2	16.68379892963558	16.018568218280535
3	6.4342832510630705	9.266595002815551
4	3.070630284627239	5.896390971249135
5	1.6989067037728476	4.077916103768627
6	1.0240521490181176	2.9496607955970746
7	0.6916315280332531	2.3241897131187095
8	0.48024313934454976	1.8443778953214485
9	0.3406060857887575	1.4716131677808193
>10	1.8319722228463307	15.566986727341256
>50	0.10923492730977812	3.5737214437334233
>100	0.04022808085811946	3.179457023020704
>500	0.001305873451827309	0.3950399740267569
>1k	6.913378841542124E-4	0.7033589088395834
>5k	7.68153204602893E-5	0.2835045110290038
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8134	0.14984527287351063	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	7242	0.13341276938160362	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8422089116487658E-5
2	0.0	0.0	0.0	0.0	1.8422089116487658E-5
3	0.0	0.0	0.0	1.8422089116487658E-5	1.8422089116487658E-5
4	0.0	0.0	0.0	1.8422089116487658E-5	1.8422089116487658E-5
5	0.0	0.0	0.0	1.8422089116487658E-5	3.6844178232975316E-5
6	0.0	0.0	0.0	1.8422089116487658E-5	7.368835646595063E-5
7	0.0	0.0	0.0	3.6844178232975316E-5	7.368835646595063E-5
8	0.0	0.0	0.0	7.368835646595063E-5	7.368835646595063E-5
9	0.0	0.0	0.0	1.1053253469892596E-4	7.368835646595063E-5
10-11	0.0	0.0	0.0	1.289546238154136E-4	1.1053253469892596E-4
12-13	0.0	0.0	0.0	2.1185402483960808E-4	2.210650693978519E-4
14-15	0.0	0.0	0.0	2.3027611395609574E-4	2.579092476308272E-4
16-17	0.0	0.0	0.0	2.579092476308272E-4	3.131755149802902E-4
18-19	0.0	0.0	0.0	2.855423813055587E-4	3.500196932132655E-4
20-21	0.0	0.0	0.0	3.22386559538534E-4	3.684417823297532E-4
22-23	0.0	0.0	0.0	3.5923073777150935E-4	3.9607491600448467E-4
24-25	0.0	0.0	0.0	4.3291909423746E-4	4.3291909423746E-4
26-27	0.0	1.8422089116487658E-5	0.0	4.789743170286791E-4	4.421301387957038E-4
28-29	0.0	1.8422089116487658E-5	0.0	9.303155003826267E-4	4.5134118335394765E-4
30-31	0.0	1.8422089116487658E-5	0.0	0.002100118159279593	4.605522279121915E-4
32-33	0.0	1.8422089116487658E-5	0.0	0.005020019284242887	4.7897431702867915E-4
34-35	0.0	1.8422089116487658E-5	0.0	0.008547849350050273	4.7897431702867915E-4
36-37	0.0	5.526626734946298E-5	0.0	0.014203430708811984	4.7897431702867915E-4
38-39	0.0	9.21104455824383E-5	0.0	0.023174988108541475	5.250295398198982E-4
40-41	0.0	9.21104455824383E-5	0.0	0.032874218028372225	5.342405843781421E-4
42-43	0.0	9.21104455824383E-5	0.0	0.041808931249868744	5.342405843781421E-4
44-45	0.0	9.21104455824383E-5	0.0	0.05241084353640739	5.710847626111174E-4
46-47	0.0	9.21104455824383E-5	0.0	0.06408123699170232	6.816172973100434E-4
48-49	0.0	9.21104455824383E-5	0.0	0.07529107821908507	7.092504309847748E-4
50-51	0.0	9.21104455824383E-5	0.0	0.0865009194464678	7.184614755430187E-4
52-53	0.0	9.21104455824383E-5	0.0	0.09683571144081737	7.368835646595064E-4
54-55	0.0	9.21104455824383E-5	0.0	0.1091140338369564	7.737277428924817E-4
56-57	0.0	9.21104455824383E-5	0.0	0.12187133055012411	8.013608765672132E-4
58-59	0.0	9.21104455824383E-5	0.0	0.1346286272632918	8.289940102419447E-4
60-61	1.8422089116487658E-5	9.21104455824383E-5	0.0	0.14731223561999357	8.289940102419447E-4
62-63	1.8422089116487658E-5	9.21104455824383E-5	0.0	0.15971030159538976	9.026823667078953E-4
64-65	1.8422089116487658E-5	9.21104455824383E-5	0.0	0.17318605978410048	9.118934112661392E-4
66-67	1.8422089116487658E-5	9.21104455824383E-5	0.0	0.18797899734464008	9.303155003826267E-4
68-69	1.8422089116487658E-5	9.21104455824383E-5	0.0	0.20312195459839294	9.395265449408706E-4
70-71	1.8422089116487658E-5	9.21104455824383E-5	0.0	0.2179885805153985	9.671596786156021E-4
72-73	1.8422089116487658E-5	9.21104455824383E-5	0.0	0.23365656730897122	0.0010500590796397964
74-75	1.8422089116487658E-5	9.21104455824383E-5	0.0	0.24891005709742303	0.0011145363915475034
76-77	1.8422089116487658E-5	9.21104455824383E-5	0.0	0.265001751940675	0.0011329584806639911
78-79	1.8422089116487658E-5	9.21104455824383E-5	0.0	0.28265011331427015	0.0011421695252222348
80-81	1.8422089116487658E-5	9.21104455824383E-5	0.0	0.30032610782154007	0.001188224748013454
82-83	1.8422089116487658E-5	9.21104455824383E-5	0.0	0.3180665796407177	0.0013171793718288675
84-85	1.8422089116487658E-5	9.21104455824383E-5	0.0	0.33671894487116144	0.0013632345946200867
86-87	1.8422089116487658E-5	9.21104455824383E-5	0.0	0.35723194110237044	0.001400078772853062
88-89	1.8422089116487658E-5	9.21104455824383E-5	0.0	0.3796424125125777	0.0014553450402025252
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	18450	0.0	25.928144	1
TACATGG	18890	0.0	24.465975	2
GTATCAA	20250	0.0	24.070477	1
ACATGGG	19585	0.0	22.65275	3
TCAACGC	23390	0.0	20.389622	4
TATCAAC	23670	0.0	20.247646	2
CAACGCA	23680	0.0	20.027239	5
ATCAACG	24060	0.0	19.841208	3
CATGGGG	15345	0.0	19.780607	4
AACGCAG	24685	0.0	19.231987	6
GAGTACA	14425	0.0	19.091764	1
AGAGTAC	17920	0.0	19.08839	10-11
TAGATCG	455	0.0	18.780123	5
GTACCTG	5115	0.0	18.537086	1
AGTACAT	14045	0.0	18.498955	2
GTATATA	1390	0.0	17.481932	1
ACGCAGA	27505	0.0	17.192421	7
GGTATCA	15345	0.0	16.767216	1
GAGTACT	8660	0.0	16.580313	12-13
CGCAGAG	28430	0.0	16.532848	8
>>END_MODULE