FastQCFastQC Report
Mon 15 Aug 2016
SRR2921433_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921433_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5186340
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56640.10920996309536206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78750.043.373821
GGTATCA46350.035.456251
GTACCTG62700.034.7270551
TATCAAC102850.032.6259042
TCAACGC102750.032.3004344
CAACGCA105150.031.6503815
TACCTGG67200.031.5158122
ATCAACG107100.031.1169153
AACGCAG114950.029.461946
GTACATG163450.026.4456981
TACATGG169850.024.6411532
ACGCAGA138250.024.1601897
ACCTGGG86150.023.9978793
ACATGGG174300.023.117623
GTATAGG11950.023.1130711
CGCAGAG146850.022.6475378
GAGTACA125600.019.901441
CATGGGG142300.019.7472744
TAACGCA4700.019.5066974
GCAGAGT169250.019.3522519
Produced by FastQC (version 0.11.5)