##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921433_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5186340
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.9794120709402	31.0	31.0	34.0	26.0	34.0
2	30.067831264436965	31.0	31.0	34.0	26.0	34.0
3	30.20088733095015	31.0	31.0	34.0	26.0	34.0
4	33.70225072016104	37.0	35.0	37.0	28.0	37.0
5	33.66818353598106	37.0	35.0	37.0	28.0	37.0
6	33.76699117296591	37.0	35.0	37.0	29.0	37.0
7	33.63880405064072	37.0	35.0	37.0	29.0	37.0
8	33.61281404612887	37.0	35.0	37.0	29.0	37.0
9	35.016673800792084	39.0	35.0	39.0	28.0	39.0
10-11	34.870771102550165	39.0	35.0	39.0	28.0	39.0
12-13	34.68434204082262	38.0	35.0	39.0	27.0	39.0
14-15	35.623385373886016	39.0	36.0	41.0	27.0	41.0
16-17	35.456574000161964	39.0	36.0	40.5	27.0	41.0
18-19	35.25130882279218	39.0	35.5	40.0	26.0	41.0
20-21	35.119120516587806	39.0	35.0	40.0	25.0	41.0
22-23	35.153039523054794	39.0	35.0	40.0	25.0	41.0
24-25	34.993643108627666	39.0	35.0	40.0	24.5	41.0
26-27	34.69258889698709	39.0	34.0	40.0	23.5	41.0
28-29	34.429988006956734	38.5	34.0	40.0	21.5	41.0
30-31	34.15107021521921	38.0	34.0	40.0	19.0	41.0
32-33	33.85191830847958	38.0	33.0	40.0	17.5	41.0
34-35	33.407416308996325	38.0	33.0	40.0	13.5	41.0
36-37	33.00023330518246	38.0	32.0	40.0	9.0	41.0
38-39	32.74091266673608	38.0	32.0	40.0	9.0	41.0
40-41	32.44197757956478	37.5	31.0	40.0	8.5	41.0
42-43	32.132038682384874	37.0	31.0	40.0	7.5	41.0
44-45	31.790645522661453	37.0	30.0	40.0	4.5	41.0
46-47	31.4728196570221	37.0	30.0	39.0	2.0	40.0
48-49	31.228135737340786	36.0	30.0	39.0	2.0	40.0
50-51	30.530416343702882	35.5	28.5	38.5	2.0	39.5
52-53	31.105905995364747	36.5	30.5	39.0	2.0	40.0
54-55	31.51495399453179	37.0	31.0	39.0	2.0	40.5
56-57	31.344387949883732	36.0	30.5	39.0	2.0	41.0
58-59	30.98323904718935	36.0	30.0	39.0	2.0	40.5
60-61	30.50531309169858	35.0	29.0	39.0	2.0	40.0
62-63	29.98365060910006	35.0	28.5	38.5	2.0	40.0
64-65	29.40353977949768	34.5	28.0	38.0	2.0	40.0
66-67	28.888122548849477	34.0	26.5	37.5	2.0	40.0
68-69	28.34162386191418	34.0	26.0	37.0	2.0	39.0
70-71	27.786863568528098	34.0	26.0	36.0	2.0	39.0
72-73	27.197045122379173	33.0	24.5	36.0	2.0	38.5
74-75	26.566337340012417	33.0	23.0	35.0	2.0	37.5
76-77	25.936340463602463	32.0	21.0	35.0	2.0	37.0
78-79	25.33041104131237	32.0	19.0	35.0	2.0	36.0
80-81	24.703507097490714	31.0	15.5	34.5	2.0	36.0
82-83	24.02618551811104	31.0	7.5	34.0	2.0	35.0
84-85	23.35608810837701	30.5	2.0	34.0	2.0	35.0
86-87	22.67512754659355	30.0	2.0	34.0	2.0	35.0
88-89	21.967740159727285	29.0	2.0	34.0	2.0	35.0
90-91	21.163351515712428	29.0	2.0	34.0	2.0	35.0
92-93	20.286629684903033	28.0	2.0	33.5	2.0	35.0
94-95	19.33172815511517	26.5	2.0	33.0	2.0	34.0
96-97	18.410447059005	25.0	2.0	32.5	2.0	34.0
98	17.778138147518288	24.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	199403.0
3	33861.0
4	26325.0
5	22138.0
6	20773.0
7	21849.0
8	24788.0
9	28607.0
10	31878.0
11	35036.0
12	37699.0
13	40905.0
14	35383.0
15	36665.0
16	40269.0
17	45095.0
18	50470.0
19	51489.0
20	54040.0
21	58664.0
22	64913.0
23	70995.0
24	78242.0
25	86829.0
26	97873.0
27	110458.0
28	126471.0
29	145252.0
30	170505.0
31	202232.0
32	246734.0
33	313130.0
34	408024.0
35	545298.0
36	689893.0
37	664445.0
38	259585.0
39	10124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.49382958848487	16.797535981876386	12.885310427182633	24.82332400245611
2	16.179307862206745	18.28899142124781	38.985141853542146	26.546558863003295
3	20.66767633894119	21.897553634820188	27.098819262231828	30.335950764006792
4	13.980040722667214	14.144972540945497	34.01409589235078	37.860890844036504
5	15.813619554391353	35.506373638416946	32.300722743159334	16.379284064032365
6	35.94284702145099	32.42501339046599	16.354370385691727	15.277769202391298
7	32.18625438157719	29.732108186062252	18.600939327586204	19.48069810477436
8	28.468084072334204	33.75070441495084	18.271492032830654	19.5097194798843
9	27.150688693228076	16.51849403484792	18.487902502652528	37.842914769271474
10-11	27.167197267827483	24.765698696000683	25.5184269199873	22.54867711618454
12-13	27.079880922249945	21.660753757336902	26.474570633112776	24.784794687300383
14-15	24.03924627709017	23.31522179324496	23.83104874013106	28.814483189533814
16-17	24.65399541202887	26.55888762479109	24.318956462678308	24.468160500501735
18-19	24.723383312632418	25.778358226520087	25.80637101980072	23.69188744104677
20-21	25.14167101601438	24.963142715631395	25.81268153038759	24.082504737966637
22-23	24.969718341733834	24.614946916501566	25.721396390984147	24.69393835078045
24-25	24.631534973871442	24.901151412505143	25.82444251596343	24.64287109765998
26-27	24.66994384461915	25.098027787823828	25.756219877128984	24.475808490428033
28-29	24.661426057581597	25.163839797313024	25.71919099673343	24.455543148371948
30-31	24.812430664750202	25.06279964886218	25.61830161195412	24.506468074433503
32-33	24.609921855139017	25.126714879850876	25.59233588595487	24.671027379055232
34-35	24.75599373291714	25.123964622055894	25.71081875265303	24.409222892373936
36-37	24.804243591415478	25.131503673137352	25.59773867630438	24.466514059142792
38-39	24.793633922415008	25.105304450454973	25.61664466863792	24.484416958492094
40-41	24.883905473498	25.07008533979256	25.58202906089378	24.463980125815656
42-43	24.813816395015404	24.997445422691897	25.596748289693526	24.591989892599173
44-45	24.77433840796064	25.046706487456955	25.5731322687789	24.605822835803508
46-47	24.86612186254312	25.078283770088987	25.5248317630703	24.530762604297593
48-49	24.837579034823246	25.18941561596211	25.38964411349441	24.583361235720236
50-51	24.64948782555867	25.365341796621326	25.354437662665447	24.630732715154554
52-53	24.785903672756042	25.29624257550412	25.38062000433819	24.537233747401647
54-55	24.70068460112001	25.138304089200442	25.511879808737607	24.64913150094194
56-57	24.678300444203185	25.251291048809037	25.496274073122677	24.5741344338651
58-59	24.71858106359757	25.222457460807192	25.500528075411765	24.558433400183475
60-61	24.821547461323934	25.070772805989705	25.478278004363407	24.629401728322954
62-63	24.668446635493464	25.253726985430564	25.490002773385694	24.58782360569028
64-65	24.69323777844979	25.068322627265466	25.539248703671042	24.6991908906137
66-67	24.678939726207012	25.26118333283549	25.45515785439216	24.60471908656534
68-69	24.669383390220126	25.226279729855356	25.503341717313216	24.60099516261131
70-71	24.605906201863647	25.041778927299095	25.584970379082062	24.767344491755196
72-73	24.777323668397862	25.076863437065555	25.430105902027062	24.71570699250952
74-75	24.613537971762206	25.26581037371797	25.42866783257215	24.691983821947677
76-77	24.7087926473467	25.150094425601083	25.4261405050232	24.714972422029017
78-79	24.700724177898902	25.023620602802627	25.538310026864774	24.737345192433697
80-81	24.643155412976235	25.188791529850857	25.42960478596976	24.738448271203143
82-83	24.784521883377646	25.170074024073564	25.298352452392546	24.747051640156243
84-85	24.804851897774622	25.139824506154834	25.32942378629927	24.725899809771274
86-87	24.73397535687118	25.10496353694315	25.470044416123834	24.691016690061836
88-89	24.707925021197212	24.896324243886955	25.486329892279507	24.909420842636326
90-91	24.744945940297136	24.98527370188239	25.403731075423636	24.866049282396837
92-93	24.72001702947269	25.09851216797661	25.451856293873842	24.729614508676857
94-95	24.81451610013133	24.91378501253477	25.424813121356898	24.846885765977
96-97	24.797813644537868	24.928029947909604	25.416808392109036	24.85734801544349
98	24.822321663595684	25.01014460836582	25.284431575560816	24.883102152477676
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	812.0
1	623.5
2	800.0
3	1095.5
4	823.5
5	678.5
6	1117.0
7	2328.0
8	3406.0
9	3313.0
10	2525.5
11	2013.0
12	2143.5
13	2536.5
14	2911.0
15	3236.5
16	3550.0
17	3782.5
18	3813.5
19	3712.0
20	3436.5
21	3163.0
22	3121.5
23	3167.5
24	3385.0
25	3959.5
26	4788.0
27	6177.5
28	8514.0
29	11543.0
30	15772.5
31	21648.0
32	28830.5
33	37450.0
34	47578.5
35	58730.5
36	71145.0
37	84691.5
38	98062.0
39	112486.0
40	127232.5
41	141724.5
42	158169.0
43	174298.0
44	188522.0
45	202174.5
46	215963.5
47	229453.0
48	237141.0
49	238490.0
50	238636.0
51	239040.5
52	239345.5
53	239089.0
54	235299.5
55	226583.5
56	212908.0
57	197565.0
58	181716.5
59	163361.0
60	142681.5
61	119918.5
62	97407.0
63	75326.5
64	56338.5
65	42389.5
66	32290.0
67	24706.0
68	18877.0
69	14353.0
70	10755.0
71	8278.0
72	6344.5
73	4667.5
74	3568.0
75	2732.5
76	1876.0
77	1199.0
78	774.0
79	479.5
80	292.5
81	169.5
82	100.0
83	58.5
84	48.5
85	36.0
86	23.5
87	19.0
88	15.0
89	10.5
90	9.5
91	8.0
92	8.5
93	11.0
94	9.0
95	6.0
96	3.5
97	3.5
98	3.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4885719023434638
2	0.052426181083384434
3	0.0605436589193921
4	0.057574320233536556
5	0.06914317225635033
6	0.06841047829490546
7	0.04664175507197754
8	0.02286776416509523
9	0.013921185267452577
10-11	0.016765194723060966
12-13	0.012581126574809982
14-15	0.016967649633460206
16-17	0.015878249401311908
18-19	0.05772857159384074
20-21	0.08731591064218698
22-23	0.10409074607526696
24-25	0.07312478549420208
26-27	0.08958147749665468
28-29	0.09166387086076115
30-31	0.06150772992129324
32-33	0.035979129790950845
34-35	0.4098747864582731
36-37	0.6411457791043395
38-39	0.5895294176625521
40-41	0.5843137935422669
42-43	0.5578307631200423
44-45	0.5410944905270383
46-47	0.5310874335273044
48-49	0.5202994790160306
50-51	0.5349822803749851
52-53	0.6192806487812215
54-55	0.5879097783793581
56-57	0.5442662841232931
58-59	0.5213503164081028
60-61	0.5351654538653463
62-63	0.5472934670692627
64-65	0.5663627914868674
66-67	0.5932121688898143
68-69	0.5454424507456125
70-71	0.49253423416127756
72-73	0.4740915558949086
74-75	0.45394247195517456
76-77	0.4693001230154598
78-79	0.4628215658826841
80-81	0.45954372447622027
82-83	0.48031945456718994
84-85	0.44505373731764597
86-87	0.44805199813355856
88-89	0.47614502712895795
90-91	0.4590327668452126
92-93	0.4536146878145282
94-95	0.46467258220633434
96-97	0.4584350428240339
98	0.4530940894735015
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	5186340.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.28038651475745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.01605267925675	40.17619947726704
2	13.74597993096643	14.922742073539135
3	5.029073680916827	8.189401896340781
4	2.3652454133210785	5.135457409493016
5	1.2818105392437737	3.4788585754420858
6	0.8302388750235088	2.7039412221512085
7	0.5412339707428915	2.0564872388788755
8	0.3717156618047701	1.614149583708143
9	0.2807608725568917	1.3715827812547776
>10	1.4230450351903925	13.736612734289794
>50	0.0811994367721073	3.015302816864054
>100	0.0326800517042516	2.9577653465106493
>500	6.425690972775799E-4	0.24701990344100636
>1k	2.8558524726387613E-4	0.28472706338906845
>5k	3.569815590512117E-5	0.1097518774303189
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5664	0.10920996309536206	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.928142003802296E-5
2	0.0	0.0	0.0	1.928142003802296E-5	1.928142003802296E-5
3	0.0	0.0	0.0	1.928142003802296E-5	1.928142003802296E-5
4	0.0	0.0	0.0	1.928142003802296E-5	1.928142003802296E-5
5	0.0	0.0	0.0	1.928142003802296E-5	1.928142003802296E-5
6	0.0	0.0	0.0	1.928142003802296E-5	1.928142003802296E-5
7	0.0	0.0	0.0	1.928142003802296E-5	1.928142003802296E-5
8	0.0	0.0	0.0	1.928142003802296E-5	1.928142003802296E-5
9	0.0	0.0	0.0	1.928142003802296E-5	1.928142003802296E-5
10-11	0.0	0.0	0.0	3.856284007604592E-5	1.928142003802296E-5
12-13	0.0	1.928142003802296E-5	0.0	5.784426011406888E-5	5.784426011406888E-5
14-15	9.64071001901148E-6	2.892213005703444E-5	0.0	5.784426011406888E-5	7.712568015209184E-5
16-17	1.928142003802296E-5	3.856284007604592E-5	0.0	5.784426011406888E-5	7.712568015209184E-5
18-19	1.928142003802296E-5	3.856284007604592E-5	0.0	5.784426011406888E-5	9.64071001901148E-5
20-21	2.892213005703444E-5	3.856284007604592E-5	0.0	9.64071001901148E-5	1.1568852022813776E-4
22-23	3.856284007604592E-5	3.856284007604592E-5	0.0	1.928142003802296E-4	1.5425136030418368E-4
24-25	3.856284007604592E-5	3.856284007604592E-5	0.0	2.41017750475287E-4	1.7353278034220666E-4
26-27	3.856284007604592E-5	4.8203550095057395E-5	0.0	3.0850272060836735E-4	1.7353278034220666E-4
28-29	4.8203550095057395E-5	5.784426011406888E-5	0.0	7.326939614448725E-4	1.7353278034220666E-4
30-31	5.784426011406888E-5	5.784426011406888E-5	0.0	0.0018702977436882273	1.7353278034220666E-4
32-33	5.784426011406888E-5	5.784426011406888E-5	0.0	0.004087661048060867	1.928142003802296E-4
34-35	5.784426011406888E-5	6.748497013308036E-5	0.0	0.007066640443935415	1.928142003802296E-4
36-37	5.784426011406888E-5	8.676639017110333E-5	0.0	0.011617055572908834	1.928142003802296E-4
38-39	5.784426011406888E-5	9.64071001901148E-5	0.0	0.01896327660739558	2.1209562041825256E-4
40-41	5.784426011406888E-5	9.64071001901148E-5	0.0	0.026916862373080053	2.3137704045627551E-4
42-43	5.784426011406888E-5	9.64071001901148E-5	0.0	0.03491865168885958	2.3137704045627551E-4
44-45	5.784426011406888E-5	9.64071001901148E-5	0.0	0.04337355437553265	2.41017750475287E-4
46-47	5.784426011406888E-5	9.64071001901148E-5	0.0	0.05222372617298519	2.5065846049429847E-4
48-49	5.784426011406888E-5	9.64071001901148E-5	0.0	0.06148844850125522	2.6993988053232145E-4
50-51	5.784426011406888E-5	1.0604781020912628E-4	0.0	0.07190041532178762	2.6993988053232145E-4
52-53	5.784426011406888E-5	1.1568852022813776E-4	0.0	0.08156040676083712	3.1814343062737887E-4
54-55	5.784426011406888E-5	1.8317349036121812E-4	0.0	0.09204949926152162	3.470655606844133E-4
56-57	5.784426011406888E-5	2.3137704045627551E-4	0.0	0.10221080762155971	3.470655606844133E-4
58-59	5.784426011406888E-5	2.3137704045627551E-4	0.0	0.1129409178727195	3.6634698072243624E-4
60-61	7.712568015209184E-5	2.5065846049429847E-4	0.0	0.12431695569515303	3.856284007604592E-4
62-63	7.712568015209184E-5	2.6029917051330993E-4	0.0	0.13573155635766262	4.049098207984822E-4
64-65	8.676639017110333E-5	2.988620105893559E-4	0.0	0.14697262423983	4.241912408365051E-4
66-67	9.64071001901148E-5	3.0850272060836735E-4	0.0	0.15874393117304303	4.241912408365051E-4
68-69	9.64071001901148E-5	3.2778414064639033E-4	0.0	0.17192278176903172	4.241912408365051E-4
70-71	9.64071001901148E-5	3.470655606844133E-4	0.0	0.18508235094498238	4.6275408091255103E-4
72-73	9.64071001901148E-5	3.470655606844133E-4	0.0	0.19763455538973534	5.495204710836545E-4
74-75	9.64071001901148E-5	3.470655606844133E-4	0.0	0.21042777758496356	5.784426011406889E-4
76-77	1.1568852022813776E-4	3.470655606844133E-4	0.0	0.22476351338323364	5.784426011406889E-4
78-79	1.1568852022813776E-4	3.470655606844133E-4	0.0	0.2395812846824543	5.784426011406889E-4
80-81	1.1568852022813776E-4	3.470655606844133E-4	0.0	0.25476540296239736	6.073647311977232E-4
82-83	1.3496994026616072E-4	3.470655606844133E-4	0.0	0.2701326947327017	6.748497013308036E-4
84-85	1.5425136030418368E-4	3.470655606844133E-4	0.0	0.28623268046445083	6.748497013308036E-4
86	1.7353278034220666E-4	3.6634698072243624E-4	0.0	0.2980521909477589	6.748497013308036E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7875	0.0	43.37382	1
GGTATCA	4635	0.0	35.45625	1
GTACCTG	6270	0.0	34.727055	1
TATCAAC	10285	0.0	32.625904	2
TCAACGC	10275	0.0	32.300434	4
CAACGCA	10515	0.0	31.650381	5
TACCTGG	6720	0.0	31.515812	2
ATCAACG	10710	0.0	31.116915	3
AACGCAG	11495	0.0	29.46194	6
GTACATG	16345	0.0	26.445698	1
TACATGG	16985	0.0	24.641153	2
ACGCAGA	13825	0.0	24.160189	7
ACCTGGG	8615	0.0	23.997879	3
ACATGGG	17430	0.0	23.11762	3
GTATAGG	1195	0.0	23.113071	1
CGCAGAG	14685	0.0	22.647537	8
GAGTACA	12560	0.0	19.90144	1
CATGGGG	14230	0.0	19.747274	4
TAACGCA	470	0.0	19.506697	4
GCAGAGT	16925	0.0	19.352251	9
>>END_MODULE