FastQCFastQC Report
Mon 15 Aug 2016
SRR2921433_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921433_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5186340
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61850.11925558293517202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85700.044.417661
GGTATCA46050.037.968741
TCAACGC109800.034.2241674
GTACCTG66850.033.352941
CAACGCA113100.033.2485
TATCAAC114050.033.23722
ATCAACG114250.032.8901983
TACCTGG68600.031.9915072
AACGCAG121500.030.9508676
ACGCAGA146800.025.3589177
GTACATG167400.024.8170851
CGCAGAG157750.023.6287638
TACATGG171200.023.584792
ACCTGGG90800.022.9157713
ACATGGG176250.021.9671973
GAGTACA131350.021.6537931
TATACCG4700.021.2140885
AGTACAT128050.020.2919882
GCAGAGT183800.019.9690749
CATGGGG138400.019.0852344
Produced by FastQC (version 0.11.5)