##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921433_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5186340
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86273210009371	33.0	31.0	34.0	30.0	34.0
2	31.97878176131916	34.0	31.0	34.0	30.0	34.0
3	32.10597454081298	34.0	31.0	34.0	30.0	34.0
4	35.65550793044806	37.0	35.0	37.0	35.0	37.0
5	35.45169907873375	37.0	35.0	37.0	33.0	37.0
6	35.48870417288492	37.0	35.0	37.0	33.0	37.0
7	35.418394281902074	37.0	35.0	37.0	33.0	37.0
8	35.37021772579507	37.0	35.0	37.0	33.0	37.0
9	36.97942884577564	39.0	37.0	39.0	33.0	39.0
10-11	36.88554038107799	39.0	37.0	39.0	33.0	39.0
12-13	36.75556047231767	39.0	37.0	39.0	33.0	39.0
14-15	38.034915181033256	40.0	38.0	41.0	33.0	41.0
16-17	37.929327521913336	40.0	38.0	41.0	33.0	41.0
18-19	37.81762476042836	40.0	38.0	41.0	33.0	41.0
20-21	37.697229356347634	40.0	38.0	41.0	32.0	41.0
22-23	37.65727536181585	40.0	38.0	41.0	32.5	41.0
24-25	37.71033744413209	40.0	38.0	41.0	32.0	41.0
26-27	37.59607661665066	40.0	38.0	41.0	32.0	41.0
28-29	37.45460546358318	40.0	37.5	41.0	32.0	41.0
30-31	37.30968091177979	40.0	37.0	41.0	32.0	41.0
32-33	37.141362598672664	40.0	37.0	41.0	31.0	41.0
34-35	36.98830967503095	40.0	37.0	41.0	31.0	41.0
36-37	36.87947521759082	40.0	36.5	41.0	30.5	41.0
38-39	36.83795537893775	40.0	37.0	41.0	30.5	41.0
40-41	36.6628821095416	40.0	36.0	41.0	30.0	41.0
42-43	36.417186782972195	39.0	36.0	41.0	30.0	41.0
44-45	36.19884301839062	39.0	35.0	40.5	30.0	41.0
46-47	35.91030090584111	39.0	35.0	40.0	29.0	41.0
48-49	35.559286799554215	39.0	35.0	40.0	28.0	41.0
50-51	35.270540689580706	38.0	34.5	40.0	27.0	41.0
52-53	34.941909612559144	38.0	34.0	40.0	26.5	41.0
54-55	34.59509056482992	38.0	34.0	40.0	26.0	41.0
56-57	34.188477905420775	37.5	33.0	40.0	25.0	41.0
58-59	33.77019863718923	37.0	33.0	39.5	23.5	41.0
60-61	33.31692195266797	36.0	32.5	39.0	22.5	40.5
62-63	32.853554529784006	36.0	32.0	39.0	22.0	40.0
64-65	32.311432435976045	35.0	31.0	38.5	20.0	40.0
66-67	31.755561532795767	35.0	31.0	38.0	18.0	40.0
68-69	31.22322013211629	35.0	30.0	37.0	16.0	39.5
70-71	30.6420893153939	34.0	29.5	36.5	12.0	39.0
72-73	30.064586683480066	34.0	29.0	36.0	8.0	39.0
74-75	29.519346205609352	34.0	29.0	35.5	7.0	38.0
76-77	28.30202435628979	32.0	27.0	34.5	6.5	36.0
78-79	29.23480710867394	33.5	29.0	35.0	4.5	37.0
80-81	29.245215219210465	34.0	29.0	35.0	2.0	36.0
82-83	28.878715144012926	34.0	29.0	35.0	2.0	36.0
84-85	28.346436504355673	33.0	28.5	35.0	2.0	35.5
86-87	27.777076319716794	33.0	27.0	35.0	2.0	35.0
88-89	27.195429917822587	33.0	26.0	35.0	2.0	35.0
90-91	26.665894349387045	32.0	25.0	34.0	2.0	35.0
92-93	26.171117300446944	32.0	24.5	34.0	2.0	35.0
94-95	25.607803383503587	32.0	23.5	34.0	2.0	35.0
96-97	24.758326006393716	31.5	19.0	34.0	2.0	35.0
98-99	23.64646957199104	31.0	2.0	34.0	2.0	35.0
100-101	20.074541295017298	25.5	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36457.0
3	18593.0
4	11089.0
5	8563.0
6	7253.0
7	7522.0
8	8982.0
9	10584.0
10	12541.0
11	15014.0
12	17527.0
13	20431.0
14	23274.0
15	25928.0
16	30010.0
17	34747.0
18	35685.0
19	33000.0
20	34207.0
21	35922.0
22	38947.0
23	42817.0
24	48477.0
25	56230.0
26	66601.0
27	80460.0
28	97732.0
29	117994.0
30	143547.0
31	177002.0
32	225553.0
33	299694.0
34	416692.0
35	607838.0
36	896800.0
37	1025752.0
38	405974.0
39	10901.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.714325507977534	17.287891332270693	13.298468716631332	24.699314443120443
2	16.306335488996094	18.59367106668672	38.650917602779614	26.449075841537578
3	20.70942854856484	22.062628169467636	27.37856982397819	29.849373457989337
4	14.210511084100249	14.490150682024272	33.94734811213366	37.351990121741814
5	15.79593908120488	35.481384274072106	32.45730975795141	16.265366886771606
6	36.047834848181985	32.58927985713062	16.163545772302694	15.199339522384697
7	32.22585611743178	29.76544170158146	18.60101316403142	19.40768901695534
8	28.499744056750686	33.872023742886284	18.171410946032648	19.456821254330382
9	26.94912218672851	16.56887024482429	18.499735212424483	37.982272356022726
10-11	27.07221129124044	24.87786481496296	25.567843568145232	22.482080325651367
12-13	26.992202555607044	21.6636400850518	26.588472844624516	24.755684514716634
14-15	23.978604101597618	23.330807747547926	23.957313162604507	28.733274988249946
16-17	24.514476145378197	26.62789066014849	24.499903799370333	24.35772939510298
18-19	24.588489273022148	25.747553387990536	26.036328446281782	23.62762889270553
20-21	25.031396637582365	24.91649502142821	26.044507765430208	24.007600575559216
22-23	24.807378740183758	24.599832141781746	25.999881947564273	24.592907170470223
24-25	24.505869426489603	24.904661179386274	26.037115571877173	24.55235382224695
26-27	24.562793600393736	25.061453824099928	25.991447957395614	24.38430461811072
28-29	24.514880345669994	25.188488621445345	25.92808454905352	24.368546483831143
30-31	24.67051530258429	25.04847270136815	25.860025897741618	24.420986098305946
32-33	24.43561737078112	25.074955674485377	25.872033998802564	24.617392955930942
34-35	24.607144988603263	25.104125800762816	25.973779552285333	24.31494965834859
36-37	24.578662911527815	25.158961063253233	25.847429968379892	24.41494605683906
38-39	24.625104594280256	25.07964014360778	25.875617320793527	24.419637941318438
40-41	24.71092910860202	25.093734152846043	25.789820465697446	24.405516272854495
42-43	24.647597481021812	25.056629189607975	25.79986832237128	24.49590500699893
44-45	24.574026867109033	25.084170636476376	25.830053897362255	24.511748599052336
46-47	24.62797102455507	25.135523463475973	25.789920309811997	24.44658520215696
48-49	24.661493123715278	25.249911086057413	25.595627005504713	24.492968784722592
50-51	24.53783379313257	25.372153925628798	25.530051433110057	24.559960848128572
52-53	24.66401720102356	25.307821541115917	25.51087953942412	24.517281718436397
54-55	24.617936547878326	25.152267159521145	25.597899390969925	24.631896901630604
56-57	24.629787207785984	25.293672228993476	25.575529309954863	24.501011253265677
58-59	24.646051356705918	25.289432203267275	25.578666769502195	24.48584967052461
60-61	24.770319530410685	25.145498122034176	25.585128296250133	24.499054051305002
62-63	24.56468149794883	25.312940464994117	25.613013494560192	24.509364542496865
64-65	24.61944705270862	25.137315105833068	25.590547271404297	24.652690570054013
66-67	24.61359816948646	25.35250646779844	25.505168210981633	24.528727151733467
68-69	24.611793400610804	25.282339745732408	25.595469097383905	24.510397756272884
70-71	24.572449235397343	25.126578861098793	25.641282758354706	24.659689145149162
72-73	24.731403878724148	25.190885852699985	25.45603429204287	24.621675976533
74-75	24.54612667140911	25.359080770204606	25.46763283859222	24.627159719794065
76-77	24.635009990625388	25.23420575060049	25.54466847196136	24.586115786812762
78-79	24.615894329176534	25.124057011858703	25.6401117977198	24.61993686124496
80-81	24.510130870294887	25.389215250784105	25.503621398684835	24.597032480236177
82-83	24.62340139061586	25.35625104992442	25.397794787437437	24.62255277202228
84-85	24.678399790176883	25.331483881419082	25.414236729845026	24.57587959855901
86-87	24.569435567020758	25.259699632382965	25.57260750187399	24.598257298722288
88-89	24.589695991985444	25.144980802983124	25.551708268926497	24.71361493610493
90-91	24.63012757603601	25.2573888085174	25.45472746857226	24.657756146874334
92-93	24.617450959115516	25.388707635548162	25.521303094971127	24.4725383103652
94-95	24.654838850878853	25.214046288106374	25.43536709365047	24.695747767364306
96-97	24.615537837274214	25.236406135043026	25.50282310589952	24.645232921783244
98-99	24.618548570210965	25.231614880392534	25.46476037770323	24.685076171693275
100-101	25.08940039595529	24.654175454049884	25.61908267721622	24.637341472778605
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	369.0
1	326.0
2	606.5
3	897.0
4	631.5
5	475.5
6	933.0
7	2102.5
8	3085.5
9	2919.5
10	2081.0
11	1426.5
12	1225.0
13	1234.0
14	1316.0
15	1465.0
16	1532.0
17	1603.0
18	1756.5
19	1867.5
20	1930.5
21	2030.0
22	2250.5
23	2652.0
24	3049.5
25	3575.0
26	4402.0
27	5575.5
28	7393.0
29	9881.5
30	13627.0
31	18501.0
32	24531.0
33	32060.0
34	40820.0
35	50816.5
36	62644.0
37	75497.0
38	88454.5
39	101420.0
40	115745.0
41	129866.5
42	144054.5
43	160345.5
44	176267.5
45	191430.0
46	203579.0
47	215738.5
48	230229.0
49	237785.0
50	236488.5
51	235434.5
52	236592.0
53	235701.5
54	232969.0
55	225499.5
56	211097.5
57	194208.0
58	178318.5
59	162026.5
60	141230.5
61	118589.5
62	96649.0
63	74301.0
64	55044.0
65	40864.5
66	30949.5
67	24107.5
68	18496.5
69	13962.0
70	10646.5
71	8196.0
72	6031.0
73	4458.0
74	3429.0
75	2578.0
76	1848.5
77	1149.5
78	716.5
79	419.0
80	237.0
81	140.0
82	74.5
83	28.5
84	12.0
85	9.0
86	9.5
87	5.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3243134850395462
2	0.0
3	0.006459275712737691
4	0.06247180092319439
5	5.013169209885969E-4
6	0.06601958221019061
7	0.07637370477060894
8	0.10612493588927838
9	0.09309069594357486
10-11	0.09820991296366996
12-13	0.09692769853114142
14-15	0.0652483254086697
16-17	0.03591164482081777
18-19	0.03910271983711056
20-21	0.028334046745874742
22-23	0.04247696834376458
24-25	0.03479332245861243
26-27	0.02209650736357431
28-29	0.024660936228631365
30-31	0.0033453263765969832
32-33	0.005302390510456314
34-35	0.00820424422617877
36-37	0.0107493916711978
38-39	0.01594573437144499
40-41	0.02050579021043742
42-43	0.003653829097205351
44-45	0.01432609508825106
46-47	0.02429458924790893
48-49	0.002805446615532341
50-51	0.0073462210344867485
52-53	0.0026415545452091456
54-55	0.004338319508555166
56-57	0.0038755654276426154
58-59	0.0012532923024714925
60-61	0.0053313126405133485
62-63	0.014827412009239656
64-65	0.007066640443935415
66-67	0.03861104362614098
68-69	0.061285993590855986
70-71	0.012340108824334693
72-73	0.032720569804524965
74-75	0.051143966650855896
76-77	0.30836775066810124
78-79	0.07605556133998156
80-81	0.033568952286197974
82-83	0.027823089114867132
84-85	0.01966704843878342
86-87	0.11994971405654084
88-89	0.5345966519742247
90-91	0.13340814524308087
92-93	0.04873378914610303
94-95	0.00827172919631185
96-97	0.005716941041273808
98-99	0.010074541969866997
100-101	0.007731849435247206
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5186340.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.97256917477096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.28590811085822	33.93108927795828
2	15.82709647103038	15.501871535266174
3	6.07179896689201	8.920547847642654
4	2.8300400356520807	5.5437732565337114
5	1.5586557161201293	3.8165687438672595
6	0.983681659647509	2.890405087382468
7	0.667730638015412	2.28903394022266
8	0.47178568136807314	1.848364553317153
9	0.33914650386803463	1.4947988058953148
>10	1.8113854124504871	15.79951639210235
>50	0.10875431274180945	3.636179438078166
>100	0.04271506097347231	3.5543486228407666
>500	7.493099474049652E-4	0.24051067413744326
>1k	5.126832610428443E-4	0.41353846258024324
>5k	3.943717392253644E-5	0.1194533621754943
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6185	0.11925558293517202	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.928142003802296E-5	0.0
5	0.0	1.928142003802296E-5	0.0	1.928142003802296E-5	0.0
6	0.0	1.928142003802296E-5	0.0	1.928142003802296E-5	1.928142003802296E-5
7	0.0	5.784426011406888E-5	0.0	1.928142003802296E-5	1.928142003802296E-5
8	0.0	5.784426011406888E-5	0.0	1.928142003802296E-5	3.856284007604592E-5
9	0.0	5.784426011406888E-5	0.0	1.928142003802296E-5	3.856284007604592E-5
10-11	0.0	5.784426011406888E-5	0.0	4.8203550095057395E-5	9.64071001901148E-5
12-13	0.0	5.784426011406888E-5	0.0	5.784426011406888E-5	1.3496994026616072E-4
14-15	0.0	5.784426011406888E-5	0.0	5.784426011406888E-5	1.5425136030418368E-4
16-17	0.0	5.784426011406888E-5	0.0	7.712568015209184E-5	1.5425136030418368E-4
18-19	0.0	5.784426011406888E-5	0.0	9.64071001901148E-5	2.1209562041825256E-4
20-21	0.0	7.712568015209184E-5	0.0	1.1568852022813776E-4	2.1209562041825256E-4
22-23	0.0	8.676639017110333E-5	0.0	1.928142003802296E-4	2.2173633043726402E-4
24-25	0.0	1.0604781020912628E-4	0.0	2.41017750475287E-4	2.41017750475287E-4
26-27	0.0	1.1568852022813776E-4	0.0	2.6029917051330993E-4	2.5065846049429847E-4
28-29	0.0	1.1568852022813776E-4	0.0	6.170054412167347E-4	2.5065846049429847E-4
30-31	0.0	1.3496994026616072E-4	0.0	0.001706405673365032	2.5065846049429847E-4
32-33	0.0	1.3496994026616072E-4	0.0	0.003875565427642615	2.988620105893559E-4
34-35	0.0	1.6389207032319517E-4	0.0	0.006912389083631232	3.0850272060836735E-4
36-37	0.0	2.41017750475287E-4	0.0	0.01146280421260465	3.0850272060836735E-4
38-39	0.0	2.988620105893559E-4	0.0	0.018625851756730177	3.0850272060836735E-4
40-41	0.0	3.0850272060836735E-4	0.0	0.026415545452091453	3.2778414064639033E-4
42-43	0.0	3.470655606844133E-4	0.0	0.034369131217775926	3.470655606844133E-4
44-45	9.64071001901148E-6	3.470655606844133E-4	0.0	0.042824033904449	3.759876907414477E-4
46-47	1.928142003802296E-5	3.6634698072243624E-4	0.0	0.05135606227127415	4.049098207984822E-4
48-49	1.928142003802296E-5	3.856284007604592E-4	0.0	0.06030264116891681	4.82035500950574E-4
50-51	1.928142003802296E-5	3.856284007604592E-4	0.0	0.07041574597885986	4.82035500950574E-4
52-53	1.928142003802296E-5	3.856284007604592E-4	0.0	0.07990220463756714	4.916762109695855E-4
54-55	1.928142003802296E-5	4.1455053081749366E-4	0.0	0.09009243512766228	5.013169209885969E-4
56-57	1.928142003802296E-5	4.241912408365051E-4	0.0	0.09995488147711103	5.013169209885969E-4
58-59	1.928142003802296E-5	4.434726608745281E-4	0.0	0.11037648900766243	5.013169209885969E-4
60-61	1.928142003802296E-5	4.5311337089353956E-4	0.0	0.1213476170092975	5.013169209885969E-4
62-63	1.928142003802296E-5	4.7239479093156254E-4	0.0	0.13238622998106564	5.398797610646429E-4
64-65	1.928142003802296E-5	4.82035500950574E-4	0.0	0.1433187951426247	5.398797610646429E-4
66-67	1.928142003802296E-5	4.82035500950574E-4	0.0	0.15470447367507723	5.495204710836544E-4
68-69	1.928142003802296E-5	4.82035500950574E-4	0.0	0.16763266581057162	5.784426011406889E-4
70-71	1.928142003802296E-5	5.013169209885969E-4	0.0	0.1802812773555147	6.170054412167347E-4
72-73	1.928142003802296E-5	5.109576310076084E-4	0.0	0.19240929055943112	6.748497013308037E-4
74-75	1.928142003802296E-5	5.2059834102662E-4	0.0	0.204922932164108	6.941311213688266E-4
76-77	1.928142003802296E-5	5.2059834102662E-4	0.0	0.2188633988515986	7.326939614448725E-4
78-79	1.928142003802296E-5	5.302390510456314E-4	0.0	0.2332955417500588	7.519753814828954E-4
80-81	1.928142003802296E-5	5.398797610646429E-4	0.0	0.24818079801941254	7.905382215589414E-4
82-83	1.928142003802296E-5	5.398797610646429E-4	0.0	0.2631624613889564	9.062267417870791E-4
84-85	1.928142003802296E-5	5.398797610646429E-4	0.0	0.278809333749812	9.447895818631251E-4
86-87	1.928142003802296E-5	5.398797610646429E-4	0.0	0.29415734410007827	9.544302918821366E-4
88-89	1.928142003802296E-5	5.495204710836544E-4	0.0	0.31030553338192246	9.64071001901148E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8570	0.0	44.41766	1
GGTATCA	4605	0.0	37.96874	1
TCAACGC	10980	0.0	34.224167	4
GTACCTG	6685	0.0	33.35294	1
CAACGCA	11310	0.0	33.248	5
TATCAAC	11405	0.0	33.2372	2
ATCAACG	11425	0.0	32.890198	3
TACCTGG	6860	0.0	31.991507	2
AACGCAG	12150	0.0	30.950867	6
ACGCAGA	14680	0.0	25.358917	7
GTACATG	16740	0.0	24.817085	1
CGCAGAG	15775	0.0	23.628763	8
TACATGG	17120	0.0	23.58479	2
ACCTGGG	9080	0.0	22.915771	3
ACATGGG	17625	0.0	21.967197	3
GAGTACA	13135	0.0	21.653793	1
TATACCG	470	0.0	21.214088	5
AGTACAT	12805	0.0	20.291988	2
GCAGAGT	18380	0.0	19.969074	9
CATGGGG	13840	0.0	19.085234	4
>>END_MODULE