FastQCFastQC Report
Mon 15 Aug 2016
SRR2921432_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921432_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457125
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT56560.23018771938749555No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30680.12486137253904463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG28200.034.6095961
TACCTGG29950.030.9154932
GTACATG68050.024.6253851
TACATGG70650.023.1620862
ACCTGGG39000.023.1538543
ACATGGG73100.021.3825073
AGTACAT50300.018.7724462
GTATAGG4700.018.6108191
GAGTACA53450.018.6044061
CATGGGG57900.018.1288264
TAACGCA2553.893656E-717.9751554
AGAGTAC71000.017.87182810-11
GTACGTG4400.017.7871341
GTATTAG4900.016.9116571
TCGCGTA1650.001866339216.6580319
CCTGGGG40850.016.3822524
GTATCAA110350.016.3539811
CCCTTAT5100.016.2484551
CTATACA8550.016.0830334
GTGTAGC13250.015.6353061
Produced by FastQC (version 0.11.5)