##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921432_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2457125
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.579463397263062	31.0	31.0	34.0	27.0	34.0
2	30.679940987943226	31.0	31.0	34.0	27.0	34.0
3	30.814324057587626	31.0	31.0	34.0	27.0	34.0
4	34.33201241288091	37.0	35.0	37.0	31.0	37.0
5	34.334241033728446	37.0	35.0	37.0	31.0	37.0
6	34.42938719031388	37.0	35.0	37.0	31.0	37.0
7	34.326877956961894	37.0	35.0	37.0	31.0	37.0
8	34.3162045073002	37.0	35.0	37.0	31.0	37.0
9	35.79941272829018	39.0	37.0	39.0	30.0	39.0
10-11	35.677990944701634	39.0	37.0	39.0	30.0	39.0
12-13	35.534562140713234	39.0	37.0	39.0	30.0	39.0
14-15	36.57849783792034	40.0	37.0	41.0	30.0	41.0
16-17	36.446856590527545	40.0	37.0	41.0	30.0	41.0
18-19	36.28235478455512	40.0	36.0	41.0	29.0	41.0
20-21	36.175167523019795	39.5	36.0	41.0	29.0	41.0
22-23	36.22240708144681	40.0	36.5	41.0	29.0	41.0
24-25	36.09501775448949	40.0	36.0	41.0	28.5	41.0
26-27	35.84028101948415	39.0	36.0	41.0	27.0	41.0
28-29	35.626668769395124	39.0	36.0	40.5	27.0	41.0
30-31	35.38933794576995	39.0	35.0	40.0	26.5	41.0
32-33	35.130466500483294	39.0	35.0	40.0	25.0	41.0
34-35	34.71237727018365	38.0	34.5	40.0	24.5	41.0
36-37	34.334195452001836	38.0	34.0	40.0	22.5	41.0
38-39	34.11673765070967	38.0	33.5	40.0	21.0	41.0
40-41	33.872176425700765	38.0	33.0	40.0	19.0	41.0
42-43	33.59676023808312	38.0	33.0	40.0	18.0	41.0
44-45	33.28962954672636	38.0	33.0	40.0	15.5	41.0
46-47	33.002810398331384	38.0	32.0	40.0	14.5	41.0
48-49	32.801345678384294	38.0	32.0	40.0	10.5	41.0
50-51	32.03886574757084	36.0	31.0	39.0	10.5	40.0
52-53	32.536769191636566	37.0	32.0	39.0	12.0	40.0
54-55	32.98378816706517	38.0	33.0	40.0	9.0	41.0
56-57	32.83777443150023	37.0	32.5	40.0	8.0	41.0
58-59	32.51861057129776	37.0	32.0	40.0	7.0	41.0
60-61	32.06853192247036	36.0	31.5	39.0	2.0	41.0
62-63	31.600810907056008	36.0	31.0	39.0	2.0	40.0
64-65	31.077999898255072	35.0	30.5	39.0	2.0	40.0
66-67	30.589109426667346	35.0	30.0	38.0	2.0	40.0
68-69	30.079908226077222	34.5	29.0	37.5	2.0	39.5
70-71	29.5585436231368	34.0	29.0	37.0	2.0	39.0
72-73	29.010128910820576	34.0	28.0	36.0	2.0	39.0
74-75	28.42703220226891	34.0	27.5	35.5	2.0	38.5
76-77	27.848024622271964	33.5	26.0	35.0	2.0	37.0
78-79	27.284522765427074	33.0	26.0	35.0	2.0	37.0
80-81	26.707336012616373	32.5	25.0	35.0	2.0	36.0
82-83	26.07709151956046	32.0	23.5	35.0	2.0	36.0
84-85	25.45967868952536	32.0	21.0	34.0	2.0	35.0
86-87	24.84661321666582	31.0	19.0	34.0	2.0	35.0
88-89	24.191122755252582	31.0	14.0	34.0	2.0	35.0
90-91	23.44679656102152	30.5	4.5	34.0	2.0	35.0
92-93	22.640667243221245	30.0	2.0	34.0	2.0	35.0
94-95	21.736110901968765	29.0	2.0	34.0	2.0	35.0
96-97	20.839692933814923	29.0	2.0	34.0	2.0	34.5
98	20.21497522511065	29.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	67723.0
3	11972.0
4	9238.0
5	7779.0
6	7115.0
7	7561.0
8	8773.0
9	9875.0
10	11381.0
11	12419.0
12	14069.0
13	16345.0
14	13931.0
15	13418.0
16	15156.0
17	17356.0
18	19654.0
19	18776.0
20	19461.0
21	21229.0
22	23568.0
23	26027.0
24	29219.0
25	33064.0
26	37799.0
27	43281.0
28	50442.0
29	59103.0
30	70816.0
31	85856.0
32	107585.0
33	140101.0
34	191941.0
35	271974.0
36	372527.0
37	402202.0
38	180387.0
39	8002.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.98591161023199	17.173152015954706	13.102222934810573	24.738713439002733
2	15.89643838472351	19.17741785742789	38.73352302988942	26.192620727959177
3	20.30871213432366	22.69531821074995	27.925237648643662	29.07073200628273
4	13.691887632625388	15.000030541252068	35.35703741710595	35.95104440901659
5	15.296365711157971	35.707728860663195	33.069063624286585	15.926841803892247
6	34.33162110242801	33.60247382274114	17.03473590199192	15.031169172838938
7	30.698364523178313	30.353329280641827	19.839689313557393	19.108616882622464
8	27.655855395241822	33.18563429038514	19.31463844739743	19.843871866975608
9	26.752836017730452	16.70423601528824	19.307022578242343	37.23590538873896
10-11	26.435738253633474	24.862737978671852	26.436002835503547	22.265520932191126
12-13	26.927702972497407	22.049980808548277	26.73495404430844	24.287362174645875
14-15	23.825567349796426	23.625095783686092	24.639542965534154	27.909793900983328
16-17	24.116550672559587	26.642200580119784	25.079617899210906	24.161630848109727
18-19	24.102001109238852	26.224214472390784	26.338721801755728	23.33506261661464
20-21	24.668883343801653	25.29985425816264	26.164570151191878	23.866692246843822
22-23	24.373039411395652	24.998961142661592	26.165597933692386	24.462401512250366
24-25	24.3723819977152	25.19307798668624	26.224822887118155	24.209717128480403
26-27	24.294548899897922	25.228374951221085	26.386197052979405	24.09087909590159
28-29	24.13247746439458	25.745480556586376	26.07659478437553	24.04544719464351
30-31	24.42146993427322	25.33820379374659	25.98081136332169	24.259514908658506
32-33	24.270706678383807	25.416079639105533	25.982211360049472	24.331002322461188
34-35	24.59815070242656	25.317925925925927	26.09050319284802	23.99342017879949
36-37	24.52085297582312	25.253719689286964	26.082222241795012	24.143205093094906
38-39	24.383089279503306	25.458592531131885	26.033295644862832	24.125022544501977
40-41	24.540538261115923	25.441201998581796	25.910632900612974	24.107626839689306
42-43	24.38941283272662	25.368161386889938	25.777231868409828	24.465193911973614
44-45	24.480117596723368	25.27772997728152	25.81904075760183	24.423111668393283
46-47	24.720756257218294	25.39519677234231	25.760681539888967	24.123365430550425
48-49	24.5896689366006	25.444781034022824	25.65561321765999	24.30993681171659
50-51	24.253713402294302	25.568496279999238	25.896803252542867	24.280987065163593
52-53	24.4971038703661	25.70863334544235	25.548847589002282	24.245415195189267
54-55	24.331943044989416	25.314104582383283	25.80677189294712	24.547180479680186
56-57	24.496475311836328	25.40365971714508	25.71546023520293	24.384404735815664
58-59	24.806979880819625	25.362212551355594	25.60234081069575	24.228466757129024
60-61	24.540798006365147	25.42021522008448	25.896056412664407	24.142930360885963
62-63	24.260418960187078	25.50256233123441	25.86323674696478	24.373781961613734
64-65	24.34264627929014	25.52544703982579	25.754303596171123	24.37760308471295
66-67	24.436006336496803	25.462145409417698	25.607251022474582	24.494597231610914
68-69	24.354385041597727	25.496035846603448	25.637164045519523	24.512415066279303
70-71	24.53460163667085	25.230454659423664	25.71615086625265	24.518792837652835
72-73	24.59337856892812	25.205621296935476	25.770773149162956	24.43022698497345
74-75	24.262031659194474	25.742057091222065	25.58498774397063	24.410923505612832
76-77	24.552590626378233	25.272477711573664	25.53650582822694	24.638425833821156
78-79	24.492797655143704	25.323356190066505	25.72153277642451	24.462313378365284
80-81	24.56297882806381	25.41135282900713	25.581677771215023	24.44399057171404
82-83	24.63414793747858	25.313625458772066	25.616024447121895	24.436202156627463
84-85	24.50212400788155	25.418755906409963	25.575871777422865	24.503248308285624
86-87	24.508061682176884	25.42201405190525	25.61613796044378	24.453786305474086
88-89	24.447061354289694	25.300669718077152	25.47292500269916	24.779343924933993
90-91	24.504324554741064	25.22021444394128	25.51876767823391	24.75669332308375
92-93	24.72637239032101	25.422314045869754	25.53452906694099	24.316784496868245
94-95	24.737194096791086	25.244946449239997	25.54212694544862	24.4757325085203
96-97	24.453535936723107	25.252306970141053	25.791554084997255	24.50260300813859
98	24.642185862595284	25.396922747309315	25.2708266124993	24.690064777596103
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	407.0
1	311.5
2	427.0
3	599.5
4	443.5
5	385.5
6	627.0
7	1247.5
8	1792.5
9	1726.0
10	1349.0
11	1080.5
12	1101.0
13	1316.5
14	1505.5
15	1696.0
16	1842.5
17	1921.0
18	1955.5
19	1884.5
20	1810.5
21	1733.0
22	1658.5
23	1771.0
24	2011.0
25	2277.5
26	2899.0
27	3876.5
28	5110.0
29	7070.5
30	9673.5
31	12862.0
32	16873.5
33	21210.5
34	26262.0
35	32056.0
36	38135.5
37	44701.5
38	51281.5
39	57195.0
40	63237.0
41	70101.5
42	77157.0
43	84255.0
44	90722.5
45	97371.5
46	107861.0
47	118006.0
48	120354.5
49	115976.0
50	112676.0
51	111397.5
52	109163.0
53	107895.5
54	105170.0
55	100171.0
56	93606.0
57	86169.5
58	79001.5
59	70740.0
60	61712.0
61	51840.0
62	41615.0
63	31867.0
64	23557.5
65	17697.5
66	13446.0
67	10427.0
68	8082.0
69	6192.0
70	4663.0
71	3539.0
72	2772.5
73	2117.0
74	1663.0
75	1280.5
76	894.0
77	554.5
78	330.0
79	223.0
80	145.5
81	81.5
82	51.0
83	33.5
84	26.5
85	16.0
86	9.0
87	6.5
88	6.0
89	6.0
90	4.5
91	4.5
92	5.0
93	3.5
94	3.5
95	3.0
96	3.0
97	3.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.49667802818334433
2	0.051808516050261995
3	0.0612097471638602
4	0.058198097369893675
5	0.06833189194688914
6	0.06731444269217073
7	0.04655847789591494
8	0.02250597751437147
9	0.013633820013226841
10-11	0.017032100523986366
12-13	0.012290786996998525
14-15	0.01699140255379763
16-17	0.015912906343796104
18-19	0.056448084651777995
20-21	0.08542503942615863
22-23	0.10194841532278577
24-25	0.07203540723406421
26-27	0.08835529327974767
28-29	0.09083786946126061
30-31	0.061250445134048946
32-33	0.036404334333825096
34-35	0.41715419443455254
36-37	0.655013481202625
38-39	0.5996235437757542
40-41	0.5939054789642366
42-43	0.5658238795340083
44-45	0.5502162079666276
46-47	0.5391463600752913
48-49	0.5291143104237677
50-51	0.5443557002594496
52-53	0.6274813043699445
54-55	0.5985043495955639
56-57	0.5524952942971969
58-59	0.5296637330213155
60-61	0.5432161570941649
62-63	0.5554051991656916
64-65	0.5742483593630767
66-67	0.6009462278068881
68-69	0.5535737905071985
70-71	0.49993386579844334
72-73	0.4823930406470977
74-75	0.4635498804497126
76-77	0.4778552169710536
78-79	0.4721778501297248
80-81	0.46825049600651164
82-83	0.48882331993691813
84-85	0.4541689983212087
86-87	0.45844228519102614
88-89	0.4849977107391769
90-91	0.46792491224500177
92-93	0.4628987129266928
94-95	0.4737854199521799
96-97	0.46774177137915246
98	0.46281731698631534
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2457125.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.891429431786186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.34548627766026	49.90151050962229
2	12.387116152147879	15.580868826922945
3	3.5793479326045445	6.753309237456255
4	1.525300976028722	3.837134347845798
5	0.8477856642287028	2.665922613755972
6	0.507739522550922	1.9159478611344076
7	0.34816451026476497	1.5327594609578206
8	0.24886226751410664	1.2521042988478186
9	0.18227919190704625	1.0317419041234508
>10	0.9628501938980297	10.721826734115774
>50	0.049764204829049974	2.1035384861012187
>100	0.01380954660993262	1.3806899583962366
>500	7.143111876746469E-4	0.29674378118434985
>1k	7.143111876693145E-4	0.794910673952293
>5k	6.49373806972104E-5	0.23099130558334202
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	5656	0.23018771938749555	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3068	0.12486137253904463	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.139594037747367E-5	0.0
3	0.0	0.0	0.0	8.139594037747367E-5	0.0
4	0.0	0.0	0.0	8.139594037747367E-5	0.0
5	0.0	0.0	0.0	8.139594037747367E-5	0.0
6	0.0	0.0	0.0	8.139594037747367E-5	4.069797018873684E-5
7	0.0	0.0	0.0	8.139594037747367E-5	4.069797018873684E-5
8	0.0	0.0	0.0	8.139594037747367E-5	4.069797018873684E-5
9	0.0	0.0	0.0	1.220939105662105E-4	4.069797018873684E-5
10-11	0.0	0.0	0.0	1.8314086584931573E-4	4.069797018873684E-5
12-13	0.0	0.0	0.0	2.44187821132421E-4	1.2209391056621052E-4
14-15	0.0	2.034898509436842E-5	0.0	2.44187821132421E-4	1.6279188075494735E-4
16-17	0.0	4.069797018873684E-5	0.0	2.44187821132421E-4	1.8314086584931576E-4
18-19	0.0	4.069797018873684E-5	0.0	3.255837615098947E-4	2.0348985094368417E-4
20-21	0.0	8.139594037747367E-5	0.0	4.0697970188736834E-4	2.44187821132421E-4
22-23	0.0	8.139594037747367E-5	0.0	4.273286869817368E-4	2.44187821132421E-4
24-25	0.0	8.139594037747367E-5	0.0	5.494225975479473E-4	2.44187821132421E-4
26-27	0.0	8.139594037747367E-5	0.0	7.529124484916315E-4	2.44187821132421E-4
28-29	0.0	8.139594037747367E-5	0.0	0.0015261738820776314	2.44187821132421E-4
30-31	0.0	8.139594037747367E-5	0.0	0.004090146003968052	2.44187821132421E-4
32-33	0.0	8.139594037747367E-5	0.0	0.010255888487561683	2.44187821132421E-4
34-35	0.0	1.2209391056621052E-4	0.0	0.017907106883044206	3.255837615098947E-4
36-37	0.0	1.6279188075494735E-4	0.0	0.029913008088721573	3.255837615098947E-4
38-39	0.0	1.6279188075494735E-4	0.0	0.048654423360634885	3.662817316986315E-4
40-41	0.0	1.6279188075494735E-4	0.0	0.06877956961896525	3.662817316986315E-4
42-43	0.0	1.8314086584931576E-4	0.0	0.08802970951823777	4.0697970188736834E-4
44-45	0.0	2.0348985094368417E-4	4.069797018873684E-5	0.10931474792694715	4.0697970188736834E-4
46-47	0.0	2.2383883603805258E-4	4.069797018873684E-5	0.13027420257414662	4.4767767207610517E-4
48-49	0.0	2.44187821132421E-4	4.069797018873684E-5	0.1533499516711604	4.88375642264842E-4
50-51	0.0	2.44187821132421E-4	4.069797018873684E-5	0.176303606857608	4.88375642264842E-4
52-53	0.0	2.44187821132421E-4	4.069797018873684E-5	0.20106832171745434	4.88375642264842E-4
54-55	0.0	2.6453680622678943E-4	4.069797018873684E-5	0.22477488935239354	5.697715826423157E-4
56-57	0.0	2.8488579132115787E-4	4.069797018873684E-5	0.2521239253192247	5.901205677366841E-4
58-59	0.0	3.459327466042631E-4	4.069797018873684E-5	0.2786183039120924	6.104695528310525E-4
60-61	0.0	3.662817316986315E-4	4.069797018873684E-5	0.30545861525156437	6.104695528310525E-4
62-63	0.0	3.662817316986315E-4	4.069797018873684E-5	0.33254311441216866	6.511675230197894E-4
64-65	0.0	3.662817316986315E-4	4.069797018873684E-5	0.36062471384239714	6.511675230197894E-4
66-67	0.0	4.0697970188736834E-4	4.069797018873684E-5	0.38648827389733936	6.511675230197894E-4
68-69	0.0	4.0697970188736834E-4	4.069797018873684E-5	0.41452917535737904	6.511675230197894E-4
70-71	0.0	4.0697970188736834E-4	4.069797018873684E-5	0.44405555272930763	6.511675230197894E-4
72-73	0.0	4.0697970188736834E-4	4.069797018873684E-5	0.47158772956198813	6.918654932085263E-4
74-75	0.0	4.0697970188736834E-4	4.069797018873684E-5	0.5010937579488223	6.918654932085263E-4
76-77	0.0	4.476776720761052E-4	4.069797018873684E-5	0.5310474640077326	6.918654932085263E-4
78-79	0.0	4.476776720761052E-4	4.069797018873684E-5	0.5642570076817419	6.918654932085263E-4
80-81	0.0	4.476776720761052E-4	4.069797018873684E-5	0.5986467924912244	6.918654932085263E-4
82-83	0.0	4.476776720761052E-4	4.069797018873684E-5	0.6306964440148548	7.529124484916315E-4
84-85	0.0	4.476776720761052E-4	4.069797018873684E-5	0.6652490207050923	7.732614335859999E-4
86	0.0	4.476776720761052E-4	4.069797018873684E-5	0.6896678028183344	7.732614335859999E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	2820	0.0	34.609596	1
TACCTGG	2995	0.0	30.915493	2
GTACATG	6805	0.0	24.625385	1
TACATGG	7065	0.0	23.162086	2
ACCTGGG	3900	0.0	23.153854	3
ACATGGG	7310	0.0	21.382507	3
AGTACAT	5030	0.0	18.772446	2
GTATAGG	470	0.0	18.610819	1
GAGTACA	5345	0.0	18.604406	1
CATGGGG	5790	0.0	18.128826	4
TAACGCA	255	3.893656E-7	17.975155	4
AGAGTAC	7100	0.0	17.871828	10-11
GTACGTG	440	0.0	17.787134	1
GTATTAG	490	0.0	16.911657	1
TCGCGTA	165	0.0018663392	16.658031	9
CCTGGGG	4085	0.0	16.382252	4
GTATCAA	11035	0.0	16.353981	1
CCCTTAT	510	0.0	16.248455	1
CTATACA	855	0.0	16.083033	4
GTGTAGC	1325	0.0	15.635306	1
>>END_MODULE