FastQCFastQC Report
Mon 15 Aug 2016
SRR2921432_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921432_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457125
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57530.23413542249580305No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31560.12844279391565344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG28600.029.329351
TACCTGG30050.027.0282292
GTACATG68300.024.9814781
TACATGG70550.023.5633452
ACATGGG74700.021.3009513
GAGTACA51700.020.7418651
ACCTGGG37700.020.4102573
CATGGGG56100.019.3890384
AGAGTAC72000.018.95749710-11
AGTACAT49900.018.6561262
TATAACG1901.8676752E-417.4988842
GTATCAA112800.016.8585261
TACACCG4055.18412E-1016.4110135
GAGTACT38950.016.1476312-13
TAGACTG8750.015.7344775
GTACTTT40550.015.50638114-15
GTGTAGC14950.015.302271
AGTACTT46200.015.15480912-13
GTATAGT5701.8189894E-1215.0505861
TCAACGC126300.014.7423394
Produced by FastQC (version 0.11.5)