##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921432_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2457125
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90748700208577	33.0	31.0	34.0	30.0	34.0
2	32.0232885994811	34.0	31.0	34.0	30.0	34.0
3	32.15052510556036	34.0	31.0	34.0	30.0	34.0
4	35.68534974818131	37.0	35.0	37.0	35.0	37.0
5	35.48390985399603	37.0	35.0	37.0	33.0	37.0
6	35.52259856539655	37.0	35.0	37.0	33.0	37.0
7	35.45807396856082	37.0	35.0	37.0	33.0	37.0
8	35.41172508521137	37.0	35.0	37.0	33.0	37.0
9	37.03532665208323	39.0	37.0	39.0	34.0	39.0
10-11	36.9407329704431	39.0	37.0	39.0	33.0	39.0
12-13	36.82276542707433	39.0	37.0	39.0	33.0	39.0
14-15	38.10906201353208	40.0	38.0	41.0	33.0	41.0
16-17	38.01507981889404	40.0	38.0	41.0	33.0	41.0
18-19	37.91984453375388	40.0	38.0	41.0	33.0	41.0
20-21	37.807918400569775	40.0	38.0	41.0	33.0	41.0
22-23	37.770981329806176	40.0	38.0	41.0	32.5	41.0
24-25	37.821450679147375	40.0	38.0	41.0	33.0	41.0
26-27	37.70994068270845	40.0	38.0	41.0	32.5	41.0
28-29	37.58078201149718	40.0	38.0	41.0	32.0	41.0
30-31	37.4483023859185	40.0	37.5	41.0	32.0	41.0
32-33	37.286552983669935	40.0	37.0	41.0	31.5	41.0
34-35	37.134150684234626	40.0	37.0	41.0	31.0	41.0
36-37	37.03283227349036	40.0	37.0	41.0	31.0	41.0
38-39	36.99704451340489	40.0	37.0	41.0	31.0	41.0
40-41	36.84326682606705	40.0	37.0	41.0	30.5	41.0
42-43	36.61615628020552	40.0	36.0	41.0	30.0	41.0
44-45	36.41001414254464	39.0	36.0	41.0	30.0	41.0
46-47	36.14099445490156	39.0	35.0	40.5	29.5	41.0
48-49	35.80922195655492	39.0	35.0	40.0	28.5	41.0
50-51	35.53735605636669	39.0	35.0	40.0	28.0	41.0
52-53	35.24011761713385	38.0	34.5	40.0	27.0	41.0
54-55	34.925079513659256	38.0	34.0	40.0	26.5	41.0
56-57	34.540150989469396	38.0	34.0	40.0	26.0	41.0
58-59	34.15399236913059	37.0	33.5	40.0	25.5	41.0
60-61	33.722171033219716	37.0	33.0	39.5	24.0	41.0
62-63	33.2956017703617	36.0	32.5	39.0	23.0	40.0
64-65	32.786353360126164	35.5	32.0	39.0	22.0	40.0
66-67	32.25723436943582	35.0	31.0	38.0	20.5	40.0
68-69	31.748398229638298	35.0	31.0	37.5	18.5	39.5
70-71	31.189454138474844	34.5	30.5	37.0	17.5	39.0
72-73	30.6348567940174	34.0	30.0	36.0	13.5	39.0
74-75	30.115417205066898	34.0	29.0	36.0	10.5	38.5
76-77	28.81830960980821	32.5	28.0	34.5	9.0	36.0
78-79	29.753696291397468	34.0	29.5	35.0	8.0	37.0
80-81	29.776251106476064	34.0	30.0	35.0	7.0	36.5
82-83	29.430685862542607	34.0	29.5	35.0	4.5	36.0
84-85	28.936473317393293	34.0	29.0	35.0	2.0	36.0
86-87	28.429384137966117	33.0	29.0	35.0	2.0	35.0
88-89	27.878034898509437	33.0	27.0	35.0	2.0	35.0
90-91	27.392744569364602	33.0	26.0	35.0	2.0	35.0
92-93	26.952494887317496	32.5	25.5	34.0	2.0	35.0
94-95	26.456177646639873	32.0	25.0	34.0	2.0	35.0
96-97	25.68119265401638	32.0	23.5	34.0	2.0	35.0
98-99	24.66318298824846	32.0	12.5	34.0	2.0	35.0
100-101	21.040771226535078	27.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17046.0
3	8801.0
4	5338.0
5	3863.0
6	3371.0
7	3497.0
8	3994.0
9	4814.0
10	5455.0
11	6731.0
12	7786.0
13	9221.0
14	10480.0
15	11484.0
16	13175.0
17	15160.0
18	15657.0
19	13920.0
20	14014.0
21	14828.0
22	15825.0
23	17468.0
24	19967.0
25	23487.0
26	27665.0
27	33395.0
28	41001.0
29	49658.0
30	61078.0
31	76033.0
32	98048.0
33	132336.0
34	186360.0
35	283151.0
36	434685.0
37	533536.0
38	228226.0
39	6571.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.34213567147737	17.578993614854628	13.41272505003834	24.66614566362966
2	16.110332197181666	19.405199165691613	38.31962150887725	26.16484712824948
3	20.395616722032877	22.78306567346663	28.069527801270993	28.7517898032295
4	13.923872276135056	15.293878099535348	35.277751760255086	35.50449786407451
5	15.361226479021129	35.70732342605047	33.047008381781076	15.884441713147329
6	34.52510062281685	33.686560180039805	16.78481846760094	15.003520729542402
7	30.801604243008523	30.25831701109342	19.81839649826351	19.121682247634546
8	27.729011855517427	33.2582322286409	19.166711822394173	19.846044093447503
9	26.636575101946036	16.71487291881388	19.267618027237212	37.38093395200287
10-11	26.396981435231893	24.871790641251504	26.47629942275489	22.254928500761707
12-13	26.918786837855585	22.02386384190826	26.806653912777083	24.25069540745907
14-15	23.74931708551749	23.612133488985908	24.68223477515594	27.95631465034066
16-17	24.073397401382344	26.645653095718757	25.174513133244346	24.106436369654553
18-19	24.092861534202182	26.213759043698825	26.419977769906318	23.27340165219268
20-21	24.6624024334789	25.213147201943002	26.302484814165112	23.821965550412983
22-23	24.310274066304892	24.992976521631498	26.2507898868426	24.445959525221006
24-25	24.363576701279776	25.149023113111813	26.313869556658908	24.173530628949496
26-27	24.273496445283005	25.197824631247723	26.50174326659462	24.026935656874656
28-29	24.102960847961715	25.68843154926917	26.225918227610862	23.98268937515825
30-31	24.380164364271273	25.31169078271947	26.100159032658166	24.207985820351094
32-33	24.15380053850213	25.380072165227478	26.180438302719107	24.285688993551283
34-35	24.46581883629135	25.306540091772767	26.206054039621673	24.02158703231421
36-37	24.37415555339833	25.285582962484032	26.254441389732953	24.085820094384683
38-39	24.31733308747942	25.446388684858633	26.20918708037693	24.027091147285013
40-41	24.466874321228474	25.404759501173146	26.040036733039056	24.088329444559324
42-43	24.3214176116407	25.416538802474854	25.899295654842387	24.362747931042062
44-45	24.385058147320656	25.223473681168457	25.984206136739314	24.407262034771573
46-47	24.555575050407306	25.43894965538213	25.91155506904366	24.093920225166894
48-49	24.467603643554813	25.470045188317215	25.84952449094463	24.212826677183337
50-51	24.181508447385948	25.587625437866198	25.9938822362012	24.23698387854666
52-53	24.42898926098316	25.70064692946086	25.629586639525925	24.240777170030057
54-55	24.311126613184513	25.311464827907315	25.83620639482234	24.541202164085828
56-57	24.44156246922102	25.424312402677373	25.810570793892147	24.323554334209458
58-59	24.744291843467376	25.334357848160714	25.682675253490096	24.238675054881817
60-61	24.547284029782134	25.389436976706943	25.939564338527454	24.123714654983473
62-63	24.265937471634537	25.485403006301354	25.896938789216584	24.35172073284752
64-65	24.28394467043597	25.565167063805315	25.77312811145361	24.377760154305104
66-67	24.41703026676946	25.453282925700176	25.640062057493772	24.489624750036594
68-69	24.303040163090078	25.541100243099834	25.704218827724905	24.45164076608518
70-71	24.520296783770632	25.27595285829505	25.756495579320383	24.447254778613935
72-73	24.586284999392383	25.26266528600517	25.786560778859187	24.36448893574326
74-75	24.23311553183965	25.75317219701123	25.601085091615683	24.412627179533434
76-77	24.40353312761322	25.333417434034533	25.629096719684807	24.633952718667437
78-79	24.350571461271745	25.32487209783981	25.867170740270534	24.457385700617916
80-81	24.438750200251885	25.476436638064488	25.61905058520369	24.465762576479936
82-83	24.51342051349379	25.335922480288797	25.71061601139062	24.440040994826795
84-85	24.37661042476543	25.468198563083877	25.70022184682393	24.454969165326766
86-87	24.412954145860454	25.468697475139507	25.68135376365774	24.436994615342304
88-89	24.26817996015452	25.406239348658584	25.550350847258585	24.77522984392831
90-91	24.409340359855584	25.29548009440692	25.589202743204492	24.705976802533
92-93	24.59838938390015	25.52092569198367	25.660325116021976	24.220359808094205
94-95	24.532759825587004	25.372853975718446	25.656521337426796	24.437864861267755
96-97	24.271572996140193	25.330063478089755	25.912890551628326	24.48547297414173
98-99	24.395951123356095	25.408577304783837	25.578711853534504	24.616759718325568
100-101	24.74916456021811	24.80012226593288	25.798213590525936	24.652499583323074
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	180.0
1	176.5
2	351.0
3	506.5
4	358.5
5	296.5
6	535.5
7	1073.5
8	1544.0
9	1476.5
10	1033.5
11	707.0
12	643.5
13	688.5
14	743.5
15	774.5
16	832.0
17	893.0
18	972.0
19	1011.0
20	1033.0
21	1153.0
22	1319.5
23	1486.0
24	1652.0
25	1978.0
26	2473.0
27	3128.5
28	4119.0
29	5487.0
30	7510.0
31	10074.5
32	13516.5
33	17805.5
34	22340.0
35	27494.5
36	33369.0
37	39442.5
38	45525.5
39	51659.5
40	58228.5
41	64646.0
42	70648.5
43	77588.5
44	85053.0
45	92024.5
46	97531.5
47	106076.0
48	119327.0
49	122180.5
50	114139.0
51	109595.0
52	108515.5
53	107059.0
54	105147.0
55	100679.0
56	93246.5
57	85219.5
58	78100.5
59	70418.5
60	60993.0
61	51521.5
62	42144.0
63	32134.5
64	23564.5
65	17577.0
66	13242.0
67	10458.0
68	8191.5
69	6196.0
70	4715.0
71	3635.5
72	2780.0
73	2167.0
74	1634.5
75	1223.0
76	901.0
77	571.5
78	338.0
79	181.5
80	107.0
81	68.0
82	38.0
83	21.0
84	9.0
85	5.0
86	4.5
87	2.0
88	0.5
89	1.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3257058554204609
2	0.0
3	0.0061453934984992626
4	0.06165742483593631
5	4.0697970188736834E-4
6	0.06788421427481305
7	0.07675637177595768
8	0.10809380882128505
9	0.09747163860202472
10-11	0.09964898000712215
12-13	0.09966932899221652
14-15	0.06741618761764258
16-17	0.038032253141374574
18-19	0.040494480337793154
20-21	0.02946533041664547
22-23	0.04383171389326957
24-25	0.03575316681080531
26-27	0.021569924200030524
28-29	0.024378084143053366
30-31	0.003357582540570789
32-33	0.005066897288497736
34-35	0.007610520425293789
36-37	0.010764613114920893
38-39	0.015526275627003103
40-41	0.019575723660782418
42-43	0.0037035152871750525
44-45	0.014162893625680418
46-47	0.02588390904003663
48-49	0.003011649793966526
50-51	0.007386681589255736
52-53	0.0022994353156636314
54-55	0.0043139848400061045
56-57	0.0037035152871750525
58-59	0.0010988451950958945
60-61	0.00604364857302742
62-63	0.014325685506435365
64-65	0.007101795797934578
66-67	0.041328788726662256
68-69	0.06306150480744772
70-71	0.013003001475301418
72-73	0.033148496718726154
74-75	0.05272422037950858
76-77	0.3125807600345933
78-79	0.07846568652388462
80-81	0.03432873785419952
82-83	0.02920079361041868
84-85	0.01933153583965
86-87	0.11924505265299894
88-89	0.5365620389683065
90-91	0.13346899323396247
92-93	0.05016024825761815
94-95	0.008058198097369894
96-97	0.005514574960573841
98-99	0.01001170066642926
100-101	0.007549473470010683
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2457125.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.860420027983366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.89534713924886	46.029877779530246
2	13.735208143990384	16.443906573420843
3	4.0783533581636195	7.323958351266321
4	1.690440324689308	4.047618714725702
5	0.9463083532393242	2.832320775044759
6	0.5817156547707207	2.0893046058857223
7	0.3968835545306951	1.663033139348451
8	0.2859895781786132	1.3695565018718059
9	0.2021106814730325	1.088858725660591
>10	1.1134535116275972	11.893581216147533
>50	0.05542001960715113	2.2448301268690707
>100	0.01713595868055984	1.6038974218336972
>500	8.168609000700316E-4	0.32036101567660885
>1k	7.487891583909318E-4	0.8144716333374751
>5k	6.80717416719029E-5	0.23442341938115344
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	5753	0.23413542249580305	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3156	0.12844279391565344	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.069797018873684E-5
7	0.0	0.0	0.0	0.0	4.069797018873684E-5
8	0.0	0.0	0.0	0.0	4.069797018873684E-5
9	0.0	0.0	0.0	0.0	4.069797018873684E-5
10-11	0.0	0.0	0.0	2.034898509436842E-5	4.069797018873684E-5
12-13	0.0	0.0	0.0	8.139594037747367E-5	1.6279188075494735E-4
14-15	0.0	0.0	0.0	8.139594037747367E-5	1.8314086584931576E-4
16-17	0.0	0.0	0.0	1.220939105662105E-4	2.0348985094368417E-4
18-19	0.0	0.0	0.0	1.424428956605789E-4	2.0348985094368417E-4
20-21	0.0	0.0	0.0	2.0348985094368417E-4	2.0348985094368417E-4
22-23	0.0	0.0	0.0	2.6453680622678943E-4	2.0348985094368417E-4
24-25	0.0	0.0	0.0	3.8663071679299996E-4	2.0348985094368417E-4
26-27	0.0	0.0	0.0	6.30818537925421E-4	2.0348985094368417E-4
28-29	0.0	0.0	0.0	0.0013837309864170523	2.0348985094368417E-4
30-31	0.0	0.0	0.0	0.004008750063590578	2.0348985094368417E-4
32-33	0.0	0.0	0.0	0.010032049651523631	2.0348985094368417E-4
34-35	0.0	0.0	0.0	0.017581523121534312	2.0348985094368417E-4
36-37	0.0	4.069797018873684E-5	0.0	0.029709518237777892	2.0348985094368417E-4
38-39	0.0	4.069797018873684E-5	0.0	0.04828814162893626	2.8488579132115787E-4
40-41	0.0	4.069797018873684E-5	0.0	0.06839293890217225	2.8488579132115787E-4
42-43	0.0	4.069797018873684E-5	0.0	0.08784656865238846	2.8488579132115787E-4
44-45	0.0	4.069797018873684E-5	0.0	0.10905021112072036	2.8488579132115787E-4
46-47	0.0	6.104695528310526E-5	0.0	0.12986722287225924	3.052347764155263E-4
48-49	0.0	8.139594037747367E-5	0.0	0.15286157602889555	4.0697970188736834E-4
50-51	0.0	8.139594037747367E-5	0.0	0.17550999643892762	4.476776720761052E-4
52-53	0.0	8.139594037747367E-5	0.0	0.1997456376863204	4.883756422648421E-4
54-55	0.0	1.0174492547184209E-4	0.0	0.22335046039578776	5.290736124535789E-4
56-57	0.0	1.220939105662105E-4	0.0	0.25053670448186394	5.494225975479473E-4
58-59	0.0	1.6279188075494735E-4	0.0	0.2768479422088823	5.697715826423157E-4
60-61	0.0	1.6279188075494735E-4	0.0	0.3030981329806176	5.697715826423157E-4
62-63	0.0	1.6279188075494735E-4	0.0	0.32989774634990077	5.697715826423157E-4
64-65	0.0	2.0348985094368417E-4	0.0	0.3572874802869207	5.697715826423157E-4
66-67	0.0	2.0348985094368417E-4	0.0	0.3826830136846925	5.697715826423157E-4
68-69	0.0	2.0348985094368417E-4	0.0	0.41015414356208985	5.697715826423157E-4
70-71	0.0	2.0348985094368417E-4	0.0	0.4389276084855268	6.104695528310526E-4
72-73	0.0	2.0348985094368417E-4	0.0	0.46607315460141424	6.918654932085262E-4
74-75	0.0	2.4418782113242105E-4	0.0	0.49507045836088925	7.32563463397263E-4
76-77	0.0	2.8488579132115787E-4	0.0	0.5243119499414967	7.732614335859999E-4
78-79	0.0	2.8488579132115787E-4	0.0	0.556972071017958	7.732614335859999E-4
80-81	0.0	2.8488579132115787E-4	0.0	0.590608943378949	7.936104186803683E-4
82-83	0.0	2.8488579132115787E-4	0.0	0.6218446354988045	8.139594037747367E-4
84-85	0.0	2.8488579132115787E-4	0.0	0.6556849977107392	8.139594037747367E-4
86-87	0.0	3.662817316986315E-4	0.0	0.6878160451747469	8.343083888691051E-4
88-89	0.0	3.662817316986315E-4	0.0	0.7222058299842296	8.546573739634736E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	2860	0.0	29.32935	1
TACCTGG	3005	0.0	27.028229	2
GTACATG	6830	0.0	24.981478	1
TACATGG	7055	0.0	23.563345	2
ACATGGG	7470	0.0	21.300951	3
GAGTACA	5170	0.0	20.741865	1
ACCTGGG	3770	0.0	20.410257	3
CATGGGG	5610	0.0	19.389038	4
AGAGTAC	7200	0.0	18.957497	10-11
AGTACAT	4990	0.0	18.656126	2
TATAACG	190	1.8676752E-4	17.498884	2
GTATCAA	11280	0.0	16.858526	1
TACACCG	405	5.18412E-10	16.411013	5
GAGTACT	3895	0.0	16.14763	12-13
TAGACTG	875	0.0	15.734477	5
GTACTTT	4055	0.0	15.506381	14-15
GTGTAGC	1495	0.0	15.30227	1
AGTACTT	4620	0.0	15.154809	12-13
GTATAGT	570	1.8189894E-12	15.050586	1
TCAACGC	12630	0.0	14.742339	4
>>END_MODULE