FastQCFastQC Report
Mon 15 Aug 2016
SRR2921431_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921431_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909720
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67620.1729535618919002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44100.11279580123384796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39380.10072332545553134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77850.037.250211
GGTATCA46700.032.724121
TCAACGC96050.029.6329334
GTACCTG46200.029.4914781
TACCTGG46650.028.6891162
TATCAAC99300.028.6634432
CAACGCA99650.028.5624035
ATCAACG101300.027.961793
AACGCAG105500.027.1016566
GTACATG113050.026.5882261
TACATGG116400.025.003842
ACATGGG121500.022.8980453
ACGCAGA124250.022.8959587
CGCAGAG130450.021.6645248
GAGTACA85350.020.8175951
ACCTGGG64000.020.7684443
CATGGGG95100.019.7598554
GCAGAGT146650.018.833559
AGTACAT83550.018.7614152
AGAGTAC116600.018.75724410-11
Produced by FastQC (version 0.11.5)