##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921431_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3909720
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.879026119517512	31.0	31.0	34.0	28.0	34.0
2	30.993167541409615	33.0	31.0	34.0	28.0	34.0
3	31.13225806451613	33.0	31.0	34.0	28.0	34.0
4	34.64485794379137	37.0	35.0	37.0	32.0	37.0
5	34.65666313700214	37.0	35.0	37.0	32.0	37.0
6	34.751466089643245	37.0	35.0	37.0	32.0	37.0
7	34.65629635881854	37.0	35.0	37.0	32.0	37.0
8	34.64531680018006	37.0	35.0	37.0	32.0	37.0
9	36.17494449730415	39.0	37.0	39.0	32.0	39.0
10-11	36.06590395220118	39.0	37.0	39.0	32.0	39.0
12-13	35.93213785130393	39.0	37.0	39.0	31.5	39.0
14-15	37.05405233622868	40.0	37.5	41.0	31.0	41.0
16-17	36.93520559017014	40.0	37.0	41.0	31.0	41.0
18-19	36.78066101920342	40.0	37.0	41.0	31.0	41.0
20-21	36.67825074429882	40.0	37.0	41.0	30.5	41.0
22-23	36.72662351779667	40.0	37.0	41.0	30.5	41.0
24-25	36.62550783687834	40.0	37.0	41.0	30.0	41.0
26-27	36.39736988838075	40.0	37.0	41.0	30.0	41.0
28-29	36.19420290967128	39.5	36.0	41.0	29.0	41.0
30-31	35.96546977788691	39.0	36.0	41.0	28.5	41.0
32-33	35.71507652721934	39.0	35.5	40.0	27.5	41.0
34-35	35.325367289729186	39.0	35.0	40.0	26.0	41.0
36-37	34.9774513264377	38.5	35.0	40.0	25.0	41.0
38-39	34.77921424040596	38.0	34.5	40.0	24.0	41.0
40-41	34.54206643954043	38.0	34.0	40.0	24.0	41.0
42-43	34.2784318314355	38.0	34.0	40.0	23.0	41.0
44-45	33.987964227617326	38.0	33.0	40.0	21.5	41.0
46-47	33.72007087464064	38.0	33.0	40.0	19.5	41.0
48-49	33.53398261256561	38.0	33.0	40.0	18.0	41.0
50-51	32.74429792926348	37.0	32.0	39.0	17.5	40.5
52-53	33.18718207441965	37.5	33.0	39.5	19.0	40.0
54-55	33.63753452932691	38.0	33.0	40.0	19.0	41.0
56-57	33.51312587602182	38.0	33.0	40.0	18.0	41.0
58-59	33.21182987528519	37.0	33.0	40.0	15.5	41.0
60-61	32.79327202459511	37.0	32.5	40.0	12.0	41.0
62-63	32.33935806144686	36.0	32.0	39.0	8.0	40.5
64-65	31.832318938440604	35.5	31.0	39.0	7.0	40.0
66-67	31.37358237930082	35.0	31.0	38.5	2.0	40.0
68-69	30.88384078655249	35.0	30.5	38.0	2.0	40.0
70-71	30.393413850608226	35.0	30.0	37.0	2.0	39.0
72-73	29.864950303346532	34.0	29.0	36.5	2.0	39.0
74-75	29.297051962800403	34.0	29.0	36.0	2.0	38.5
76-77	28.74172945888708	34.0	28.5	35.0	2.0	37.5
78-79	28.213419758959724	33.5	27.5	35.0	2.0	37.0
80-81	27.67521600012277	33.0	26.5	35.0	2.0	36.0
82-83	27.084005632116877	33.0	25.5	35.0	2.0	36.0
84-85	26.508379116663086	32.0	25.0	34.5	2.0	35.0
86-87	25.92821698229029	32.0	23.5	34.0	2.0	35.0
88-89	25.319077836776035	31.5	21.0	34.0	2.0	35.0
90-91	24.634886257839437	31.0	18.5	34.0	2.0	35.0
92-93	23.872220772843068	31.0	10.0	34.0	2.0	35.0
94-95	23.006881439080036	30.0	2.0	34.0	2.0	35.0
96-97	22.13851311602877	30.0	2.0	34.0	2.0	35.0
98	21.52599879275242	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89688.0
3	16023.0
4	12400.0
5	10955.0
6	10176.0
7	10392.0
8	11515.0
9	13662.0
10	15440.0
11	16933.0
12	19525.0
13	24560.0
14	19904.0
15	19214.0
16	21405.0
17	25286.0
18	28813.0
19	26952.0
20	27024.0
21	29254.0
22	31893.0
23	35799.0
24	39891.0
25	45984.0
26	52011.0
27	60292.0
28	70633.0
29	83899.0
30	100697.0
31	124121.0
32	157693.0
33	208178.0
34	292047.0
35	431111.0
36	623603.0
37	726939.0
38	357486.0
39	18320.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.74755464814157	17.299150036337405	13.234219321628071	24.71907599389295
2	15.945870278658688	18.990315223555474	38.95046443114606	26.11335006663978
3	19.81572575897953	22.602899248058723	28.364924421967537	29.216450570994212
4	13.735285724705651	15.007556281823078	35.06293915689554	36.19421883657573
5	15.20924070513411	35.851194096170666	33.136808631319866	15.802756567375365
6	34.504880322423276	33.63944023339137	16.793254215475436	15.062425228709921
7	30.699102690903697	30.75212491955182	19.573621441247557	18.97515094829693
8	27.80236327801212	33.7727767646812	19.126572602184606	19.298287355122074
9	26.988010427850373	16.383267244746744	19.14289741470454	37.48582491269835
10-11	26.387892442356858	25.118817854917697	26.333914850220495	22.15937485250495
12-13	26.539349333758995	22.273191619224196	26.942065126482767	24.245393920534042
14-15	23.660331528318817	23.83110333990574	24.484835383827267	28.023729747948174
16-17	24.03618294281301	26.963214741475582	25.052573118556428	23.948029197154984
18-19	24.027887150274953	26.16798523618445	26.642841732501303	23.16128588103929
20-21	24.529311652211845	25.396255280715703	26.56386241953165	23.510570647540803
22-23	24.39574120018697	25.079334312559077	26.387884776376907	24.137039710877048
24-25	24.051541760575006	25.29736467920143	26.534036733999105	24.117056826224456
26-27	24.133438402134967	25.428186088522004	26.47988536645561	23.958490142887428
28-29	24.100948924395325	25.625567091392092	26.345478732664667	23.928005251547916
30-31	24.261157074289123	25.447564588570227	26.302504867009365	23.988773470131285
32-33	24.089687314894537	25.48482758885382	26.255608335710782	24.169876760540856
34-35	24.24081531111343	25.524580202908187	26.337106861049385	23.89749762492899
36-37	24.303804378122752	25.515034452357437	26.216363714311868	23.964797455207947
38-39	24.298680478448965	25.56011822126637	26.166871409731595	23.97432989055307
40-41	24.384517844057736	25.45678896385254	26.158404974835676	24.00028821725405
42-43	24.300975380796487	25.42779109159323	26.139199467167256	24.132034060443022
44-45	24.28511239423172	25.536467936160406	26.099776631383136	24.07864303822474
46-47	24.393527184477506	25.519708919796567	26.042877078951417	24.043886816774506
48-49	24.336799221101042	25.66251929374479	25.845067307588454	24.155614177565717
50-51	24.295159533041904	25.74378107661269	25.803310826312536	24.157748564032868
52-53	24.325546391696847	25.77305336434261	25.796208636337248	24.105191607623297
54-55	24.320256172409405	25.524493475702236	25.931781975003737	24.223468376884625
56-57	24.29957302629159	25.686562353620868	25.889438529187597	24.12442609089995
58-59	24.345646209326862	25.629961545889696	25.936272592400577	24.088119652382865
60-61	24.396892823927374	25.543642644916503	25.918202319322475	24.14126221183365
62-63	24.224332010799777	25.66064512610714	25.956568869151493	24.15845399394159
64-65	24.28194930193062	25.512049831794155	25.959453842671603	24.246547023603622
66-67	24.310296217954566	25.666406434674588	25.843661866894102	24.179635480476747
68-69	24.231688779245946	25.680460678699298	25.87495242849097	24.212898113563785
70-71	24.22474860567868	25.497338359248744	25.959031350955573	24.318881684117
72-73	24.356690471787097	25.564995150253463	25.78529329624555	24.293021081713892
74-75	24.186164308172366	25.73384146893833	25.796508214586254	24.283486008303054
76-77	24.377565471738365	25.520792369319977	25.77180699170567	24.329835167235984
78-79	24.277289441212808	25.56396726390196	25.84984141091215	24.308901883973082
80-81	24.295646553358104	25.649502473337492	25.7500036944254	24.304847278879002
82-83	24.38886487530007	25.569125268289195	25.66115236692798	24.380857489482754
84-85	24.363592492594893	25.606021321466315	25.67675111156908	24.35363507436971
86-87	24.392030473608045	25.56037218053662	25.802875164926824	24.244722180928505
88-89	24.325082615141962	25.445746476903746	25.77016642298541	24.459004484968876
90-91	24.326524790433474	25.535023486485393	25.695352239456138	24.443099483624994
92-93	24.376018147339973	25.58921161505826	25.746589985400615	24.28818025220115
94-95	24.464739412854065	25.4374603314941	25.712953647395025	24.384846608256808
96-97	24.460696977076047	25.450823595627618	25.712708822306762	24.37577060498957
98	24.43032519474859	25.52990659417331	25.639253113163495	24.400515097914603
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	846.0
1	648.5
2	882.0
3	1305.0
4	1012.5
5	843.0
6	1371.0
7	2763.5
8	4058.5
9	4059.0
10	3067.0
11	2323.0
12	2369.5
13	2622.0
14	2929.0
15	3206.0
16	3452.5
17	3600.0
18	3629.5
19	3573.0
20	3365.5
21	3167.0
22	3143.0
23	3319.0
24	3695.5
25	4385.0
26	5462.0
27	7141.0
28	9673.5
29	13136.5
30	17611.5
31	22984.0
32	29310.5
33	35972.0
34	43459.0
35	52278.5
36	61805.0
37	72240.5
38	82494.0
39	92333.5
40	102240.0
41	113249.0
42	125272.5
43	137310.0
44	148415.5
45	157291.5
46	166184.0
47	175177.5
48	179595.0
49	178667.0
50	177348.0
51	175756.5
52	174381.0
53	173835.0
54	169712.0
55	161123.5
56	148683.5
57	136570.0
58	124605.0
59	111293.5
60	97286.5
61	81098.5
62	64953.5
63	48962.5
64	35521.5
65	26794.5
66	20464.5
67	15661.5
68	11847.0
69	8902.0
70	6552.5
71	4833.5
72	3685.5
73	2651.5
74	1962.0
75	1438.0
76	1009.5
77	648.5
78	355.5
79	207.0
80	125.0
81	71.5
82	53.5
83	35.5
84	24.0
85	20.0
86	13.0
87	10.0
88	9.5
89	8.0
90	5.5
91	4.0
92	4.0
93	4.5
94	2.5
95	5.5
96	7.0
97	2.5
98	1.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5061487779176003
2	0.054837686586251704
3	0.06394319797837185
4	0.06074603807945326
5	0.07243485466989964
6	0.07176984541092457
7	0.04923626244334633
8	0.02332647862251005
9	0.01465578097664283
10-11	0.017661111281626306
12-13	0.01308277830637488
14-15	0.017968038631922492
16-17	0.016382247322058868
18-19	0.05956948323665122
20-21	0.09018548642869566
22-23	0.10879295704040187
24-25	0.07634817838617598
26-27	0.09484055124152113
28-29	0.09780751562771758
30-31	0.06513254146076958
32-33	0.0377264868072394
34-35	0.42659065099291
36-37	0.665610325036064
38-39	0.6127165116683547
40-41	0.6077800967844245
42-43	0.5773175572675282
44-45	0.56208628750908
46-47	0.5520088395076885
48-49	0.5410617640137912
50-51	0.5562546678534525
52-53	0.6413630643626653
54-55	0.6112841840336393
56-57	0.5659484566669736
58-59	0.5419569687854885
60-61	0.5547200311019714
62-63	0.5686340709820652
64-65	0.5873694279897282
66-67	0.6160159806840387
68-69	0.5665751000071616
70-71	0.5119036657356537
72-73	0.4939100498245399
74-75	0.47321803095873877
76-77	0.4887818053466744
78-79	0.48205498091934973
80-81	0.4788706096600268
82-83	0.5003299469015684
84-85	0.4642020400437883
86-87	0.4660563927851611
88-89	0.49599459807863483
90-91	0.47708020011663244
92-93	0.47218215115148915
94-95	0.4829373970514512
96-97	0.4783590640761998
98	0.47115905998383517
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3909720.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.579384416790234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3477719808716	40.032762430590665
2	14.70683912309409	16.053804521104848
3	5.116465616650948	8.377606312394454
4	2.2330096461556086	4.875051675357107
5	1.2043627261908325	3.2866688105011463
6	0.7537201252569568	2.4682548275442437
7	0.4958994287112956	1.8946119886190331
8	0.36269097086025676	1.5836359938464655
9	0.25722963079032485	1.2635491412064745
>10	1.4111119255320776	13.778428928519348
>50	0.08049599172936595	2.973083705353137
>100	0.02941592360716292	2.642708668640611
>500	6.579403856838424E-4	0.2538322615924693
>1k	2.819744260833416E-4	0.3425555407028105
>5k	4.699573768055691E-5	0.1734451940271362
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6762	0.1729535618919002	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4410	0.11279580123384796	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3938	0.10072332545553134	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.557727919134874E-5	0.0
3	0.0	0.0	0.0	2.557727919134874E-5	0.0
4	0.0	0.0	0.0	2.557727919134874E-5	0.0
5	0.0	0.0	0.0	2.557727919134874E-5	0.0
6	0.0	0.0	0.0	2.557727919134874E-5	2.557727919134874E-5
7	7.673183757404622E-5	0.0	0.0	2.557727919134874E-5	2.557727919134874E-5
8	7.673183757404622E-5	0.0	0.0	2.557727919134874E-5	2.557727919134874E-5
9	7.673183757404622E-5	0.0	0.0	2.557727919134874E-5	5.115455838269748E-5
10-11	7.673183757404622E-5	0.0	0.0	2.557727919134874E-5	5.115455838269748E-5
12-13	7.673183757404622E-5	0.0	0.0	2.557727919134874E-5	1.1509775636106933E-4
14-15	7.673183757404622E-5	1.278863959567437E-5	0.0	2.557727919134874E-5	1.278863959567437E-4
16-17	7.673183757404622E-5	2.557727919134874E-5	0.0	2.557727919134874E-5	1.278863959567437E-4
18-19	7.673183757404622E-5	5.115455838269748E-5	0.0	2.557727919134874E-5	2.0461823353078992E-4
20-21	7.673183757404622E-5	5.115455838269748E-5	0.0	2.557727919134874E-5	3.069273502961849E-4
22-23	7.673183757404622E-5	5.115455838269748E-5	0.0	1.5346367514809244E-4	3.325046294875336E-4
24-25	7.673183757404622E-5	5.115455838269748E-5	0.0	2.0461823353078992E-4	3.5808190867888236E-4
26-27	7.673183757404622E-5	5.115455838269748E-5	0.0	3.069273502961849E-4	4.476023858486029E-4
28-29	7.673183757404622E-5	5.115455838269748E-5	0.0	0.0012149207615890652	4.603910254442773E-4
30-31	7.673183757404622E-5	5.115455838269748E-5	0.0	0.0048852603255476095	4.603910254442773E-4
32-33	7.673183757404622E-5	5.115455838269748E-5	0.0	0.012341037209825767	4.603910254442773E-4
34-35	1.4067503555241807E-4	5.115455838269748E-5	0.0	0.020858271180544896	5.115455838269748E-4
36-37	2.0461823353078992E-4	5.115455838269748E-5	0.0	0.034363074593577035	5.115455838269748E-4
38-39	2.0461823353078992E-4	5.115455838269748E-5	0.0	0.05340535895153617	5.115455838269748E-4
40-41	2.0461823353078992E-4	5.115455838269748E-5	0.0	0.07563201456881823	5.115455838269748E-4
42-43	2.0461823353078992E-4	5.115455838269748E-5	0.0	0.09682279037885066	5.115455838269748E-4
44-45	2.0461823353078992E-4	5.115455838269748E-5	0.0	0.12035388723489149	5.115455838269748E-4
46-47	2.0461823353078992E-4	5.115455838269748E-5	0.0	0.14453720471031173	5.243342234226492E-4
48-49	2.0461823353078992E-4	5.115455838269748E-5	0.0	0.16971803607419456	5.371228630183236E-4
50-51	2.0461823353078992E-4	5.115455838269748E-5	0.0	0.19532089254473467	5.371228630183236E-4
52-53	2.0461823353078992E-4	5.115455838269748E-5	0.0	0.21903103035511495	5.627001422096723E-4
54-55	2.0461823353078992E-4	5.115455838269748E-5	0.0	0.2437131047747665	5.882774214010211E-4
56-57	2.0461823353078992E-4	5.115455838269748E-5	0.0	0.27146445269737984	5.882774214010211E-4
58-59	2.0461823353078992E-4	5.115455838269748E-5	0.0	0.29872983231535766	5.882774214010211E-4
60-61	2.0461823353078992E-4	6.394319797837185E-5	0.0	0.32561155274546516	6.266433401880442E-4
62-63	2.0461823353078992E-4	7.673183757404622E-5	0.0	0.3535675189016093	6.90586538166416E-4
64-65	2.0461823353078992E-4	7.673183757404622E-5	0.0	0.3796819209559764	7.033751777620904E-4
66-67	2.0461823353078992E-4	7.673183757404622E-5	0.0	0.4081750099751389	7.161638173577647E-4
68-69	2.0461823353078992E-4	7.673183757404622E-5	0.0	0.4394048678677757	7.161638173577647E-4
70-71	2.0461823353078992E-4	7.673183757404622E-5	0.0	0.46990577330345906	7.289524569534391E-4
72-73	2.0461823353078992E-4	7.673183757404622E-5	0.0	0.5009565902417564	7.801070153361366E-4
74-75	2.0461823353078992E-4	7.673183757404622E-5	0.0	0.5330944415456862	8.440502133145085E-4
76-77	2.0461823353078992E-4	7.673183757404622E-5	0.0	0.5657182611542515	8.696274925058573E-4
78-79	2.0461823353078992E-4	7.673183757404622E-5	0.0	0.5997999856767237	8.95204771697206E-4
80-81	2.0461823353078992E-4	7.673183757404622E-5	0.0	0.6351733627983589	8.95204771697206E-4
82-83	2.0461823353078992E-4	7.673183757404622E-5	0.0	0.6680785324780292	9.975138884626008E-4
84-85	2.0461823353078992E-4	7.673183757404622E-5	0.0	0.7036821051123865	9.975138884626008E-4
86	2.0461823353078992E-4	7.673183757404622E-5	0.0	0.7316124939893394	9.975138884626008E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7785	0.0	37.25021	1
GGTATCA	4670	0.0	32.72412	1
TCAACGC	9605	0.0	29.632933	4
GTACCTG	4620	0.0	29.491478	1
TACCTGG	4665	0.0	28.689116	2
TATCAAC	9930	0.0	28.663443	2
CAACGCA	9965	0.0	28.562403	5
ATCAACG	10130	0.0	27.96179	3
AACGCAG	10550	0.0	27.101656	6
GTACATG	11305	0.0	26.588226	1
TACATGG	11640	0.0	25.00384	2
ACATGGG	12150	0.0	22.898045	3
ACGCAGA	12425	0.0	22.895958	7
CGCAGAG	13045	0.0	21.664524	8
GAGTACA	8535	0.0	20.817595	1
ACCTGGG	6400	0.0	20.768444	3
CATGGGG	9510	0.0	19.759855	4
GCAGAGT	14665	0.0	18.83355	9
AGTACAT	8355	0.0	18.761415	2
AGAGTAC	11660	0.0	18.757244	10-11
>>END_MODULE