FastQCFastQC Report
Mon 15 Aug 2016
SRR2921431_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921431_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909720
Sequences flagged as poor quality0
Sequence length101
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63230.1617251363268981No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41640.10650379055277615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39260.10041639810523516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79250.038.417031
GGTATCA47500.035.3078541
GTACCTG44150.031.2960511
TCAACGC98100.030.2673034
TATCAAC102750.029.2663042
CAACGCA101050.029.224685
ATCAACG103250.028.8496273
TACCTGG49100.027.4778562
AACGCAG109250.027.3371166
GTACATG109000.027.2322641
TACATGG117000.025.052172
ACGCAGA124700.023.7990327
ACATGGG119550.023.1278953
CGCAGAG133900.022.1283878
CATGGGG93050.021.1882174
ACCTGGG63350.020.4728723
GAGTACA86550.019.7628521
GCAGAGT150650.019.5412189
AGAGTAC120050.019.41996410-11
AGTACAT82100.019.21062
Produced by FastQC (version 0.11.5)