##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921431_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3909720
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05539041158958	33.0	31.0	34.0	30.0	34.0
2	32.17058715202111	34.0	31.0	34.0	30.0	34.0
3	32.29359621660886	34.0	31.0	34.0	30.0	34.0
4	35.80586205661786	37.0	35.0	37.0	35.0	37.0
5	35.62473143856849	37.0	35.0	37.0	35.0	37.0
6	35.66050254238155	37.0	35.0	37.0	35.0	37.0
7	35.59865796016083	37.0	35.0	37.0	35.0	37.0
8	35.55411947658656	37.0	35.0	37.0	35.0	37.0
9	37.20598380446682	39.0	38.0	39.0	34.0	39.0
10-11	37.12192625047318	39.0	37.0	39.0	34.0	39.0
12-13	37.011719253552684	39.0	37.0	39.0	34.0	39.0
14-15	38.34731643186724	40.0	38.0	41.0	34.0	41.0
16-17	38.26010021177987	40.0	38.0	41.0	33.5	41.0
18-19	38.17053817153146	40.0	38.0	41.0	33.5	41.0
20-21	38.07046246278506	40.0	38.0	41.0	33.0	41.0
22-23	38.03915958176033	40.0	38.0	41.0	33.5	41.0
24-25	38.09097441760535	40.0	38.0	41.0	33.5	41.0
26-27	37.99310295877966	40.0	38.0	41.0	33.0	41.0
28-29	37.86897015643064	40.0	38.0	41.0	33.0	41.0
30-31	37.74201221570854	40.0	38.0	41.0	33.0	41.0
32-33	37.59033575294394	40.0	38.0	41.0	33.0	41.0
34-35	37.45280493232252	40.0	37.5	41.0	32.0	41.0
36-37	37.36005289381337	40.0	37.0	41.0	32.0	41.0
38-39	37.336956099158	40.0	37.0	41.0	32.0	41.0
40-41	37.1910648332873	40.0	37.0	41.0	31.5	41.0
42-43	36.977946758335634	40.0	37.0	41.0	31.0	41.0
44-45	36.78618865289586	40.0	36.5	41.0	31.0	41.0
46-47	36.52806262341037	39.5	36.0	41.0	30.0	41.0
48-49	36.21833494470192	39.0	35.5	40.5	30.0	41.0
50-51	35.96920585105839	39.0	35.0	40.0	29.0	41.0
52-53	35.68600782153197	39.0	35.0	40.0	28.5	41.0
54-55	35.38557096160339	38.0	35.0	40.0	28.0	41.0
56-57	35.03224131139826	38.0	34.0	40.0	27.5	41.0
58-59	34.66418490326673	38.0	34.0	40.0	26.5	41.0
60-61	34.272366179675274	37.0	34.0	40.0	26.0	41.0
62-63	33.859364609230326	36.5	33.0	39.0	25.5	41.0
64-65	33.374693967854476	36.0	33.0	39.0	24.5	40.0
66-67	32.86793939207922	35.0	32.0	38.5	23.0	40.0
68-69	32.38393312053037	35.0	32.0	38.0	22.0	40.0
70-71	31.856819286291604	35.0	31.0	37.0	20.5	39.0
72-73	31.33122525398238	34.5	31.0	36.5	19.5	39.0
74-75	30.834304374737833	34.0	30.0	36.0	18.0	38.5
76-77	29.467129231760843	32.5	28.5	34.5	17.0	36.5
78-79	30.399396120438293	34.0	30.0	35.0	18.0	37.0
80-81	30.43731303008911	34.0	31.0	35.0	18.0	37.0
82-83	30.12262591694546	34.0	30.0	35.0	14.0	36.0
84-85	29.663925421769335	34.0	30.0	35.0	7.5	36.0
86-87	29.18332719478633	34.0	29.0	35.0	4.5	35.5
88-89	28.667463782572664	34.0	29.0	35.0	2.0	35.0
90-91	28.24222578598979	33.0	28.5	35.0	2.0	35.0
92-93	27.850726394729033	33.0	27.0	35.0	2.0	35.0
94-95	27.414185286925917	33.0	27.0	35.0	2.0	35.0
96-97	26.723487871254207	33.0	25.5	34.0	2.0	35.0
98-99	25.79133249951403	32.0	24.5	34.0	2.0	35.0
100-101	22.186928347810074	28.5	8.5	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	23323.0
3	12693.0
4	7251.0
5	5539.0
6	4631.0
7	4874.0
8	5518.0
9	6599.0
10	7974.0
11	9234.0
12	11143.0
13	12974.0
14	14368.0
15	16351.0
16	18714.0
17	22169.0
18	22542.0
19	19267.0
20	19244.0
21	20030.0
22	21684.0
23	23753.0
24	27432.0
25	32351.0
26	38534.0
27	46209.0
28	56637.0
29	69329.0
30	85561.0
31	107894.0
32	139862.0
33	189226.0
34	273400.0
35	425307.0
36	691327.0
37	934337.0
38	464988.0
39	17451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.16072263910461	17.646865615495212	13.541836399798926	24.650575345601247
2	15.984341589679058	19.110498961562463	38.674636546862686	26.230522901895785
3	19.789628073668542	22.640535501477572	28.521659146014393	29.048177278839493
4	13.898109875484687	15.200542594986116	34.997914080595834	35.90343344893336
5	15.252143838403681	35.818106434443834	33.14301148453344	15.78673824261905
6	34.64618682785172	33.649299652782574	16.63807740957272	15.066436109792985
7	30.868098146946014	30.64598866747623	19.526384875770226	18.959528309807528
8	27.86778248548873	33.82428439706633	19.034606583221798	19.27332653422314
9	26.88564831954137	16.308362379150708	19.137314901720423	37.668674399587495
10-11	26.41072262749334	25.100222509012184	26.370729637788205	22.118325225706275
12-13	26.502718354554748	22.21478591829741	26.992558561213446	24.289937165934393
14-15	23.662405534800396	23.731242451966125	24.54471846545504	28.061633547778435
16-17	24.019870735314434	26.903251063132956	25.1430943693613	23.933783832191306
18-19	24.019329891631426	26.148345608554852	26.650271842593316	23.18205265722041
20-21	24.50333946944587	25.33568032175574	26.60701972979784	23.55396047900055
22-23	24.368124212674132	24.98357858622055	26.486109159988708	24.16218804111661
24-25	24.080938793389308	25.24941560807464	26.60650210217341	24.063143496362645
26-27	24.120962912119186	25.38684641599283	26.56402098724152	23.928169684646463
28-29	24.149690364477166	25.527562364909024	26.418316945354203	23.90443032525961
30-31	24.24989190033268	25.414324507208114	26.321581579023768	24.014202013435437
32-33	24.0268986835862	25.418949043160417	26.359635604178045	24.194516669075337
34-35	24.20720931924974	25.429068439535214	26.44222388233604	23.921498358879006
36-37	24.25343459352631	25.509116921859302	26.276805203223415	23.96064328139097
38-39	24.264688765250153	25.517100151212862	26.257355030967812	23.960856052569174
40-41	24.32543267668135	25.371342204298426	26.262847462370754	24.04037765664947
42-43	24.307117426890354	25.381812796435828	26.213566033937724	24.097503742736098
44-45	24.252624103895894	25.44415094455461	26.2018999555913	24.101324995958194
46-47	24.269783165869384	25.4978506931457	26.140033187487816	24.092332953497095
48-49	24.249882948578072	25.58231883438909	26.02284910258871	24.144949114444124
50-51	24.20622861904959	25.707416257784367	25.90092233205898	24.185432791107058
52-53	24.300134194473152	25.6809317621168	25.898011433067293	24.120922610342756
54-55	24.29969161376943	25.460619399364965	25.96427129445098	24.27541769241463
56-57	24.25922254976564	25.641393246068944	25.941540960334564	24.15784324383085
58-59	24.32829577276919	25.564744894285656	25.933894310853095	24.173065022092054
60-61	24.384455006173418	25.525149871991403	25.974222298844634	24.116172822990546
62-63	24.181722624181994	25.607673252604847	26.00603743331445	24.204566689898716
64-65	24.264121512962333	25.420859186334216	25.99436799564542	24.320651305058036
66-67	24.224191157599755	25.639299135882045	25.902608064640354	24.23390164187785
68-69	24.233727028830693	25.638259549819146	25.892865940700617	24.235147480649545
70-71	24.240677784002397	25.434448392386184	25.99298835314552	24.3318854704659
72-73	24.37626505367499	25.469478673155542	25.79142371964607	24.362832553523393
74-75	24.15789720838147	25.663664047142476	25.808342035295695	24.370096709180352
76-77	24.295895622820463	25.509073269961696	25.8737078002436	24.32132330697424
78-79	24.23329223269633	25.46902465039928	25.996481403220283	24.301201713684108
80-81	24.22584535546872	25.651921552312295	25.797738998030223	24.324494094188758
82-83	24.350575669866554	25.58537288634713	25.720345281671563	24.343706162114753
84-85	24.26316782481061	25.63316523457611	25.747928611858157	24.35573832875512
86-87	24.301561134567432	25.593311160975794	25.823796978882914	24.281330725573863
88-89	24.25704286066417	25.48277764640971	25.790526095034274	24.469653397891843
90-91	24.22437339851149	25.54436682639772	25.80548412928009	24.425775645810706
92-93	24.265537088219485	25.642997714425654	25.80108018114165	24.290385016213207
94-95	24.27988160254281	25.519133559601414	25.756793062419575	24.444191775436195
96-97	24.253861862209057	25.484293885082685	25.85181074569882	24.410033507009434
98-99	24.214713356977775	25.54135581644308	25.798345230162393	24.445585596416755
100-101	24.60754947677527	24.98857883926219	25.967460563102872	24.43641112085967
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	370.0
1	342.5
2	707.5
3	1074.5
4	780.5
5	599.0
6	1120.0
7	2489.0
8	3685.5
9	3495.0
10	2343.0
11	1474.0
12	1268.0
13	1297.0
14	1373.5
15	1375.0
16	1457.5
17	1605.5
18	1734.0
19	1780.0
20	1878.0
21	2024.5
22	2144.0
23	2460.5
24	2811.0
25	3338.5
26	4231.5
27	5561.5
28	7504.0
29	10260.5
30	14083.5
31	18571.5
32	24188.0
33	30705.0
34	37384.5
35	45075.0
36	54020.5
37	63488.5
38	73490.5
39	82958.5
40	93408.5
41	104352.5
42	114851.5
43	126897.0
44	138489.0
45	149469.5
46	157990.0
47	166363.5
48	176695.0
49	180964.0
50	177627.0
51	173840.5
52	172482.0
53	170827.5
54	168375.5
55	161796.0
56	149349.5
57	135480.5
58	123538.5
59	111745.0
60	97043.5
61	81212.5
62	65727.5
63	49870.5
64	36318.5
65	27004.0
66	20470.5
67	15941.0
68	12106.5
69	9009.0
70	6741.0
71	4978.5
72	3636.0
73	2739.5
74	2029.5
75	1442.0
76	1050.0
77	664.0
78	376.0
79	180.5
80	110.5
81	73.5
82	30.5
83	19.0
84	12.5
85	7.0
86	5.5
87	3.5
88	2.0
89	1.0
90	0.5
91	1.0
92	1.5
93	1.0
94	1.5
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3240897046335799
2	0.0
3	0.006726824427324718
4	0.06573360752176627
5	6.90586538166416E-4
6	0.06798440809060495
7	0.0789826381428849
8	0.11016134147713902
9	0.09775636106933489
10-11	0.10321711017668785
12-13	0.1011965051205713
14-15	0.06802277400939198
16-17	0.03734282761936916
18-19	0.040309792005565614
20-21	0.029797530257921284
22-23	0.04381387925478039
24-25	0.03539895440082666
26-27	0.022226655617282057
28-29	0.02587141790204925
30-31	0.003414566772045057
32-33	0.006010660609966954
34-35	0.008939259077376385
36-37	0.011535352915298282
38-39	0.016906581545481518
40-41	0.021114043972458384
42-43	0.003759860041128265
44-45	0.014732512814216874
46-47	0.026037670216793016
48-49	0.002903021188218082
50-51	0.007212792731960346
52-53	0.0024426301627738047
54-55	0.004463235218890355
56-57	0.003708705482745567
58-59	0.001445116274311204
60-61	0.005550269584522677
62-63	0.015563774387935709
64-65	0.007084906336003601
66-67	0.040233060167991566
68-69	0.06426291396826371
70-71	0.01304441238758786
72-73	0.032969112877648525
74-75	0.05311122024083566
76-77	0.31697922101838494
78-79	0.0779851242544223
80-81	0.0360255977410147
82-83	0.02923483011571161
84-85	0.020653652947014108
86-87	0.12040504179327419
88-89	0.5410745526533869
90-91	0.13669776863816335
92-93	0.049862905783534375
94-95	0.00883694996061099
96-97	0.006125758366328024
98-99	0.010614570864409728
100-101	0.00792895654931811
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3909720.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.64548070596716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.43102626195417	36.37444207913221
2	16.18815760128168	16.720903621242975
3	5.650908361715906	8.75531636298562
4	2.5570283406712453	5.282358313309921
5	1.3913555876843218	3.592861407944512
6	0.8162084012628026	2.5292085143679826
7	0.555313294439286	2.0075595423611845
8	0.39223634253057976	1.6205787568273746
9	0.2967571074696001	1.379354712136187
>10	1.5906778503673786	14.697031840062651
>50	0.09461242306054676	3.319042441534687
>100	0.034431213067596604	2.890689683753896
>500	9.406567633348087E-4	0.3304763999138058
>1k	2.970494841290141E-4	0.33850497694973936
>5k	4.950824735483561E-5	0.1616713474773926
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6323	0.1617251363268981	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4164	0.10650379055277615	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3926	0.10041639810523516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.115455838269748E-5
2	0.0	0.0	0.0	0.0	5.115455838269748E-5
3	0.0	0.0	0.0	0.0	5.115455838269748E-5
4	0.0	0.0	0.0	0.0	7.673183757404622E-5
5	0.0	0.0	0.0	2.557727919134874E-5	7.673183757404622E-5
6	0.0	0.0	0.0	2.557727919134874E-5	7.673183757404622E-5
7	0.0	0.0	0.0	2.557727919134874E-5	7.673183757404622E-5
8	0.0	0.0	0.0	2.557727919134874E-5	1.278863959567437E-4
9	0.0	0.0	0.0	2.557727919134874E-5	1.278863959567437E-4
10-11	0.0	0.0	0.0	5.115455838269748E-5	1.278863959567437E-4
12-13	0.0	2.557727919134874E-5	0.0	5.115455838269748E-5	2.0461823353078992E-4
14-15	0.0	2.557727919134874E-5	0.0	7.673183757404622E-5	2.8135007110483614E-4
16-17	0.0	2.557727919134874E-5	0.0	8.952047716972059E-5	3.5808190867888236E-4
18-19	0.0	3.836591878702311E-5	0.0	1.0230911676539496E-4	3.9644782746590544E-4
20-21	0.0	6.394319797837185E-5	0.0	1.4067503555241807E-4	4.603910254442773E-4
22-23	0.0	7.673183757404622E-5	0.0	2.685614315091618E-4	4.603910254442773E-4
24-25	0.0	7.673183757404622E-5	0.0	3.069273502961849E-4	4.603910254442773E-4
26-27	0.0	7.673183757404622E-5	0.0	3.9644782746590544E-4	4.731796650399517E-4
28-29	0.0	1.0230911676539496E-4	0.0	0.001278863959567437	4.8596830463562606E-4
30-31	0.0	1.0230911676539496E-4	0.0	0.0048852603255476095	4.8596830463562606E-4
32-33	0.0	1.0230911676539496E-4	0.0	0.012213150813869023	4.8596830463562606E-4
34-35	1.278863959567437E-5	1.0230911676539496E-4	0.0	0.020653652947014108	5.115455838269748E-4
36-37	2.557727919134874E-5	1.1509775636106933E-4	0.0	0.03400499268489815	5.115455838269748E-4
38-39	2.557727919134874E-5	1.278863959567437E-4	0.0	0.05320074071800539	5.371228630183236E-4
40-41	2.557727919134874E-5	1.278863959567437E-4	0.0	0.07560643728962688	5.371228630183236E-4
42-43	2.557727919134874E-5	1.278863959567437E-4	0.0	0.09678442446006363	5.371228630183236E-4
44-45	2.557727919134874E-5	1.278863959567437E-4	0.0	0.12046898499125257	5.371228630183236E-4
46-47	2.557727919134874E-5	1.278863959567437E-4	0.0	0.14478018886262956	5.371228630183236E-4
48-49	2.557727919134874E-5	1.278863959567437E-4	0.0	0.16976919063257728	5.371228630183236E-4
50-51	2.557727919134874E-5	1.278863959567437E-4	0.0	0.1951546402299909	5.371228630183236E-4
52-53	2.557727919134874E-5	1.278863959567437E-4	0.0	0.2186601598068404	5.499115026139979E-4
54-55	2.557727919134874E-5	1.662523147437668E-4	0.0	0.24306088415538707	5.627001422096723E-4
56-57	2.557727919134874E-5	2.0461823353078992E-4	0.0	0.2707099229612351	5.88277421401021E-4
58-59	2.557727919134874E-5	2.0461823353078992E-4	0.0	0.2977195297872993	6.138547005923698E-4
60-61	5.115455838269748E-5	2.174068731264643E-4	0.0	0.3246140388570026	6.394319797837186E-4
62-63	5.115455838269748E-5	2.3019551272213866E-4	0.0	0.35246769589638133	6.90586538166416E-4
64-65	5.115455838269748E-5	2.3019551272213866E-4	0.0	0.3786204638695354	6.90586538166416E-4
66-67	5.115455838269748E-5	2.3019551272213866E-4	0.0	0.40678104825921035	7.033751777620904E-4
68-69	5.115455838269748E-5	2.3019551272213866E-4	0.0	0.4374098400908505	7.289524569534391E-4
70-71	5.115455838269748E-5	2.3019551272213866E-4	0.0	0.4678979568869382	7.417410965491135E-4
72-73	5.115455838269748E-5	2.3019551272213866E-4	0.0	0.4988720419876615	7.545297361447878E-4
74-75	5.115455838269748E-5	2.3019551272213866E-4	0.0	0.5306901773016994	8.184729341231597E-4
76-77	5.115455838269748E-5	2.3019551272213866E-4	0.0	0.5629175490827988	8.440502133145085E-4
78-79	5.115455838269748E-5	2.3019551272213866E-4	0.0	0.5966156144174007	8.440502133145085E-4
80-81	5.115455838269748E-5	2.3019551272213866E-4	0.0	0.6315669664323788	8.824161321015316E-4
82-83	6.394319797837185E-5	2.3019551272213866E-4	0.0	0.6641140542033701	9.463593300799034E-4
84-85	7.673183757404622E-5	2.3019551272213866E-4	0.0	0.6996281063605578	9.463593300799034E-4
86-87	7.673183757404622E-5	2.3019551272213866E-4	0.0	0.736970933979927	9.591479696755777E-4
88-89	7.673183757404622E-5	2.3019551272213866E-4	0.0	0.7746974207871664	9.719366092712521E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7925	0.0	38.41703	1
GGTATCA	4750	0.0	35.307854	1
GTACCTG	4415	0.0	31.296051	1
TCAACGC	9810	0.0	30.267303	4
TATCAAC	10275	0.0	29.266304	2
CAACGCA	10105	0.0	29.22468	5
ATCAACG	10325	0.0	28.849627	3
TACCTGG	4910	0.0	27.477856	2
AACGCAG	10925	0.0	27.337116	6
GTACATG	10900	0.0	27.232264	1
TACATGG	11700	0.0	25.05217	2
ACGCAGA	12470	0.0	23.799032	7
ACATGGG	11955	0.0	23.127895	3
CGCAGAG	13390	0.0	22.128387	8
CATGGGG	9305	0.0	21.188217	4
ACCTGGG	6335	0.0	20.472872	3
GAGTACA	8655	0.0	19.762852	1
GCAGAGT	15065	0.0	19.541218	9
AGAGTAC	12005	0.0	19.419964	10-11
AGTACAT	8210	0.0	19.2106	2
>>END_MODULE