FastQCFastQC Report
Mon 15 Aug 2016
SRR2921430_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921430_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3030931
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44360.14635767029998376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60300.034.0493161
GGTATCA39600.030.3414361
TCAACGC76100.026.6847234
TATCAAC78150.025.6914622
CAACGCA79100.025.5567575
ATCAACG80150.025.2219523
GTACATG89900.025.0403291
AACGCAG83650.024.1046776
TACATGG95400.023.4485032
ACATGGG98050.021.8797283
GTACCTG29950.021.3652921
ACGCAGA96900.020.7065267
GTATAAT6900.020.015341
CATGGGG75600.019.7063054
CGCAGAG101550.019.6201238
TACCTGG31850.018.9980132
TATAGCG1509.81126E-418.335965
AGAGTAC92950.017.69068110-11
GTATATA7400.017.4187561
AGTACAT68200.017.3411942
Produced by FastQC (version 0.11.5)