##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921430_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3030931 Sequences flagged as poor quality 0 Sequence length 98 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.819025243398812 31.0 31.0 34.0 28.0 34.0 2 30.93144449675694 31.0 31.0 34.0 28.0 34.0 3 31.06384572924953 33.0 31.0 34.0 28.0 34.0 4 34.59917134372244 37.0 35.0 37.0 32.0 37.0 5 34.60055309738163 37.0 35.0 37.0 32.0 37.0 6 34.699731864565706 37.0 35.0 37.0 32.0 37.0 7 34.60218262969365 37.0 35.0 37.0 32.0 37.0 8 34.59300987056452 37.0 35.0 37.0 32.0 37.0 9 36.108842794507694 39.0 37.0 39.0 32.0 39.0 10-11 35.997326564016134 39.0 37.0 39.0 32.0 39.0 12-13 35.86046366611447 39.0 37.0 39.0 31.0 39.0 14-15 36.95287009173089 40.0 37.0 41.0 31.0 41.0 16-17 36.81824462516633 40.0 37.0 41.0 31.0 41.0 18-19 36.66257611935079 40.0 37.0 41.0 30.5 41.0 20-21 36.559899911941244 40.0 37.0 41.0 30.0 41.0 22-23 36.61186414339356 40.0 37.0 41.0 30.0 41.0 24-25 36.50060707419601 40.0 37.0 41.0 30.0 41.0 26-27 36.263324206324725 39.5 36.0 41.0 29.5 41.0 28-29 36.04954484282222 39.0 36.0 41.0 29.0 41.0 30-31 35.82109539940038 39.0 36.0 40.0 27.5 41.0 32-33 35.566396265701854 39.0 35.0 40.0 27.0 41.0 34-35 35.16659303692496 38.5 35.0 40.0 25.5 41.0 36-37 34.80671433958741 38.0 34.5 40.0 24.0 41.0 38-39 34.59624946922249 38.0 34.0 40.0 24.0 41.0 40-41 34.35520818520778 38.0 34.0 40.0 23.5 41.0 42-43 34.09021337008332 38.0 33.0 40.0 22.5 41.0 44-45 33.793470719062896 38.0 33.0 40.0 20.5 41.0 46-47 33.522403017422704 38.0 33.0 40.0 19.0 41.0 48-49 33.32600824631112 38.0 33.0 40.0 18.0 41.0 50-51 32.5547114071551 37.0 32.0 39.0 16.0 40.5 52-53 33.039512446835644 37.5 32.5 39.0 18.5 40.0 54-55 33.494687110989986 38.0 33.0 40.0 18.5 41.0 56-57 33.36878701626662 38.0 33.0 40.0 17.5 41.0 58-59 33.0590333465196 37.0 33.0 40.0 14.0 41.0 60-61 32.6317022723381 36.5 32.0 39.5 10.0 41.0 62-63 32.17265503569695 36.0 31.5 39.0 8.0 40.5 64-65 31.655429965248302 35.5 31.0 39.0 6.5 40.0 66-67 31.191441837508012 35.0 31.0 38.0 2.0 40.0 68-69 30.68808643284852 35.0 30.0 37.5 2.0 40.0 70-71 30.19031165671538 34.5 29.5 37.0 2.0 39.0 72-73 29.6555221811384 34.0 29.0 36.5 2.0 39.0 74-75 29.075998430845175 34.0 28.5 36.0 2.0 38.5 76-77 28.50844707451275 34.0 27.5 35.0 2.0 37.5 78-79 27.967659276968035 33.0 26.5 35.0 2.0 37.0 80-81 27.40550131296291 33.0 26.0 35.0 2.0 36.0 82-83 26.795717883383027 32.5 25.0 35.0 2.0 36.0 84-85 26.19512222482135 32.0 24.0 34.5 2.0 35.0 86-87 25.59354567952883 32.0 22.0 34.0 2.0 35.0 88-89 24.960819299416585 31.0 19.5 34.0 2.0 35.0 90-91 24.242165691003855 31.0 15.5 34.0 2.0 35.0 92-93 23.44233883912237 30.5 4.5 34.0 2.0 35.0 94-95 22.547271778869266 30.0 2.0 34.0 2.0 35.0 96-97 21.653994927631146 29.0 2.0 34.0 2.0 35.0 98 21.022087272854446 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 69451.0 3 12626.0 4 9788.0 5 8557.0 6 7812.0 7 8297.0 8 9167.0 9 10918.0 10 12425.0 11 13551.0 12 15567.0 13 18892.0 14 15199.0 15 15011.0 16 17295.0 17 19925.0 18 22588.0 19 21314.0 20 22134.0 21 23910.0 22 26225.0 23 29270.0 24 33089.0 25 37553.0 26 43014.0 27 50029.0 28 58365.0 29 69630.0 30 83289.0 31 102831.0 32 129629.0 33 170746.0 34 235561.0 35 341335.0 36 478981.0 37 531608.0 38 244307.0 39 11042.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.170539556526066 17.64482762393607 13.510348465592923 24.67428435394494 2 15.978618282602977 18.941728449020026 38.93633449388096 26.143318774496034 3 19.78302739542417 22.240203681291074 28.480221165343806 29.49654775794095 4 13.81626924350451 15.003438270742942 35.001001934873244 36.17929055087931 5 15.348919826506634 35.81937382194182 33.2392922786076 15.592414072943944 6 34.804716543824874 33.61593989490324 16.567233266464783 15.012110294807101 7 30.726149289932565 30.71291278662724 19.554804570125576 19.00613335331462 8 27.617875299277443 33.8242335939859 19.17939816151659 19.378492945220056 9 26.724664194446152 16.301443272570562 19.3406707993535 37.63322173362979 10-11 26.390608969631213 25.143455558888473 26.35567936836985 22.11025610311047 12-13 26.390473461103337 22.263117246904702 26.938052122017865 24.4083571699741 14-15 23.691460373270942 23.883961460183524 24.491329036281844 27.933249130263686 16-17 23.97868403024811 26.965299016236777 25.175240660981174 23.880776292533934 18-19 23.924530756024495 26.267016093104527 26.62576306358754 23.18269008728343 20-21 24.530221210869378 25.44999862958828 26.551628032613817 23.468152126928523 22-23 24.400747947281705 25.14600346428466 26.356026010703975 24.09722257772966 24-25 24.057270040254842 25.31097459507433 26.52226827052099 24.10948709414984 26-27 24.03169662331761 25.56818238107079 26.493564347771244 23.906556647840357 28-29 24.05628412990698 25.65822671108563 26.414748214743327 23.870740944264064 30-31 24.25139944774801 25.557071981596884 26.224277430694485 23.96725113996062 32-33 24.13208511795589 25.543522566859604 26.143739939580552 24.180652375603955 34-35 24.284646986714375 25.505963621906368 26.27392903289931 23.93546035847994 36-37 24.27319574362453 25.4693279114387 26.188166200221 24.069310144715768 38-39 24.27357235732286 25.616623974920984 26.179889491026437 23.92991417672972 40-41 24.400542431562986 25.504157015750227 26.1334924607522 23.961808091934582 42-43 24.354998346000723 25.425170716946475 26.091233073880517 24.12859786317229 44-45 24.309976761197667 25.516934800313834 26.05389876109483 24.11918967739367 46-47 24.372077533180516 25.485212626798138 25.978249458027687 24.164460381993667 48-49 24.394272525042197 25.583604835935926 25.862874132134177 24.159248506887703 50-51 24.263626321498588 25.822986362897527 25.79304842813403 24.120338887469856 52-53 24.40873227325621 25.690436231862407 25.790384937808035 24.110446557073352 54-55 24.348498403637507 25.566344008774145 25.89671860756765 24.188438980020702 56-57 24.34754323406767 25.66729428227309 25.855346739238072 24.12981574442117 58-59 24.319567497647192 25.64523364330994 25.905763243104303 24.129435615938576 60-61 24.387191688463332 25.55261800468055 25.914157907918284 24.146032398937837 62-63 24.303019756986888 25.697834532850266 25.8895769838725 24.109568726290348 64-65 24.353186355961405 25.52330015693654 25.878850432790795 24.244663054311257 66-67 24.31929764700728 25.616958594603638 25.793403176533538 24.27034058185555 68-69 24.31739193324504 25.618844235776223 25.821033969334973 24.242729861643767 70-71 24.20232806324045 25.492294140847978 25.907632480280462 24.39774531563111 72-73 24.371818939045465 25.538098138916173 25.761544231303173 24.328538690735193 74-75 24.249065381684566 25.707132930086008 25.70214454258807 24.34165714564136 76-77 24.40738108960031 25.52435376016912 25.678520122265642 24.389745027964928 78-79 24.320523003037724 25.46477446455303 25.857368171915834 24.357334360493407 80-81 24.277546317550467 25.63045335230243 25.766373854470757 24.325626475676344 82-83 24.4232666669319 25.548684906763846 25.690916288377608 24.337132137926645 84-85 24.407252533173278 25.59599544355265 25.59879599491327 24.397956028360802 86-87 24.36342044999013 25.544996278834063 25.780380117390315 24.31120315378549 88-89 24.333164917194296 25.408889852529786 25.751258645417057 24.50668658485886 90-91 24.381422402806077 25.423293129559966 25.7548275851782 24.440456882455756 92-93 24.437461510262715 25.484349219333662 25.806850957793703 24.271338312609917 94-95 24.461268961351916 25.410867619884076 25.73597500974162 24.39188840902239 96-97 24.486830274830226 25.311296089894718 25.750262068622614 24.45161156665244 98 24.438298609994767 25.40881970700412 25.635333185948213 24.517548497052903 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 663.0 1 501.5 2 529.0 3 708.0 4 567.5 5 513.5 6 903.5 7 1846.0 8 2675.5 9 2612.0 10 1984.0 11 1529.5 12 1557.0 13 1770.5 14 1995.5 15 2190.5 16 2316.0 17 2409.0 18 2491.0 19 2440.5 20 2303.0 21 2217.5 22 2176.5 23 2249.0 24 2410.5 25 2881.5 26 3751.5 27 5108.0 28 6953.5 29 9613.5 30 13460.0 31 18159.5 32 23695.5 33 29704.5 34 36181.5 35 43450.5 36 51277.0 37 59069.0 38 66846.5 39 74439.0 40 82265.5 41 90305.5 42 98476.5 43 107039.0 44 114394.5 45 120917.0 46 127805.0 47 134144.0 48 137156.5 49 136114.5 50 134858.0 51 134002.5 52 132739.5 53 131955.5 54 128499.5 55 122125.0 56 113648.0 57 104089.5 58 95212.5 59 85351.5 60 74803.5 61 63285.0 62 51100.0 63 39269.0 64 28954.0 65 21900.0 66 16913.5 67 12936.0 68 9836.0 69 7517.5 70 5641.0 71 4123.0 72 3096.0 73 2295.5 74 1734.0 75 1316.5 76 881.5 77 502.5 78 284.0 79 160.0 80 92.5 81 57.5 82 38.5 83 26.0 84 17.5 85 15.5 86 14.0 87 9.5 88 5.5 89 7.5 90 7.5 91 4.5 92 4.5 93 4.0 94 3.0 95 4.5 96 4.5 97 2.5 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4909382628637868 2 0.05325096480256397 3 0.06219211192864503 4 0.059123747785746354 5 0.06968155989034393 6 0.06938462142490212 7 0.04718022284242036 8 0.02362310458403705 9 0.015077875411878395 10-11 0.018146239554777063 12-13 0.013444713851948462 14-15 0.018426681438805437 16-17 0.017387396809759113 18-19 0.058612353761929914 20-21 0.08723392251423738 22-23 0.10483577488237114 24-25 0.07347577361543367 26-27 0.08980738921473302 28-29 0.09221589010109435 30-31 0.06199415295168382 32-33 0.036688397063476534 34-35 0.416736639666162 36-37 0.6494209205026443 38-39 0.5956420650948504 40-41 0.5883340795286992 42-43 0.5616756039645904 44-45 0.5468946670181538 46-47 0.5360564130295279 48-49 0.5252511522037288 50-51 0.5398011370103774 52-53 0.6231088731482175 54-55 0.5934645163482771 56-57 0.5480989174613345 58-59 0.5252016624594885 60-61 0.538646376311437 62-63 0.5520251038377317 64-65 0.5705342681836043 66-67 0.5967473360495504 68-69 0.5504249354406286 70-71 0.4974214193592662 72-73 0.4798360635725459 74-75 0.4593967992013015 76-77 0.4745901506830739 78-79 0.46846991897869 80-81 0.46500563688186897 82-83 0.48562636364866113 84-85 0.4509505495176235 86-87 0.45304561535712956 88-89 0.48146922513247575 90-91 0.46355393771748676 92-93 0.45837401115366866 94-95 0.4696741694218707 96-97 0.46416431122978385 98 0.45834101799084176 >>END_MODULE >>Sequence Length Distribution pass #Length Count 98 3030931.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.23263393680243 #Duplication Level Percentage of deduplicated Percentage of total 1 76.70081831894584 46.965931307771996 2 13.542719839481801 16.585128128793215 3 4.352809140906677 7.996019061657181 4 1.8753265106060573 4.593247269436869 5 0.9607886734438918 2.9415810565807923 6 0.608919473089124 2.237144591559417 7 0.3907014979202346 1.674657726049707 8 0.2716365373189913 1.330641652281149 9 0.20209419189609704 1.1137283705814853 >10 1.0367200085728552 11.14646705203514 >50 0.04488376264134061 1.8516383894537833 >100 0.01215036284090816 1.1238373353487046 >500 2.698014601364586E-4 0.11105183648308853 >1k 1.618808760564931E-4 0.3289262219674638 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4436 0.14635767029998376 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.299316282686739E-5 2 0.0 0.0 0.0 0.0 3.299316282686739E-5 3 0.0 0.0 0.0 0.0 3.299316282686739E-5 4 0.0 3.299316282686739E-5 0.0 0.0 3.299316282686739E-5 5 0.0 6.598632565373477E-5 0.0 0.0 6.598632565373477E-5 6 0.0 6.598632565373477E-5 0.0 0.0 9.897948848060216E-5 7 0.0 6.598632565373477E-5 0.0 0.0 9.897948848060216E-5 8 0.0 6.598632565373477E-5 0.0 0.0 9.897948848060216E-5 9 0.0 6.598632565373477E-5 0.0 3.299316282686739E-5 9.897948848060216E-5 10-11 0.0 6.598632565373477E-5 0.0 6.598632565373477E-5 9.897948848060216E-5 12-13 0.0 6.598632565373477E-5 0.0 6.598632565373477E-5 3.464282096821076E-4 14-15 0.0 8.248290706716846E-5 0.0 6.598632565373477E-5 3.6292479109554127E-4 16-17 0.0 9.897948848060216E-5 0.0 6.598632565373477E-5 3.9591795392240865E-4 18-19 0.0 1.1547606989403586E-4 0.0 6.598632565373477E-5 4.2891111674927607E-4 20-21 0.0 1.6496581413433695E-4 0.0 9.897948848060216E-5 4.948974424030108E-4 22-23 0.0 1.6496581413433695E-4 0.0 1.6496581413433695E-4 6.103735122970467E-4 24-25 0.0 1.6496581413433695E-4 0.0 3.134350468552402E-4 6.268700937104804E-4 26-27 0.0 1.6496581413433695E-4 0.0 4.7840086098957713E-4 6.268700937104804E-4 28-29 0.0 1.6496581413433695E-4 0.0 0.0014022094201418642 6.268700937104804E-4 30-31 0.0 1.6496581413433695E-4 0.0 0.005295402633712216 6.763598379507815E-4 32-33 3.299316282686739E-5 1.6496581413433695E-4 0.0 0.013560189921842497 7.588427450179499E-4 34-35 3.299316282686739E-5 1.6496581413433695E-4 0.0 0.02314470372304747 7.588427450179499E-4 36-37 3.299316282686739E-5 2.1445555837463804E-4 0.0 0.037183294505879545 7.588427450179499E-4 38-39 3.299316282686739E-5 3.2993162826867384E-4 0.0 0.05854636743627618 7.588427450179499E-4 40-41 3.299316282686739E-5 3.6292479109554127E-4 0.0 0.08179005064780426 7.588427450179499E-4 42-43 3.299316282686739E-5 3.9591795392240865E-4 0.0 0.1056606039530428 7.588427450179499E-4 44-45 3.299316282686739E-5 4.2891111674927607E-4 0.0 0.12997656495644408 7.588427450179499E-4 46-47 3.299316282686739E-5 4.4540769816270976E-4 0.0 0.15689898582316786 8.413256520851184E-4 48-49 3.299316282686739E-5 5.278906052298782E-4 0.0 0.1846297391791499 9.403051405657205E-4 50-51 3.299316282686739E-5 5.608837680567456E-4 0.0 0.2115026703016334 9.897948848060216E-4 52-53 3.299316282686739E-5 5.608837680567456E-4 0.0 0.2365774740500526 0.0011382641175269248 54-55 3.299316282686739E-5 6.103735122970467E-4 0.0 0.2665517624782616 0.0011877538617672259 56-57 3.299316282686739E-5 6.268700937104804E-4 0.0 0.2963610850923363 0.0012537401874209608 58-59 3.299316282686739E-5 6.268700937104804E-4 0.0 0.3266982983116409 0.0013197265130746956 60-61 3.299316282686739E-5 6.598632565373478E-4 0.0 0.35685404913539764 0.0013362230944881292 62-63 3.299316282686739E-5 6.598632565373478E-4 0.0 0.38519517600367675 0.0013527196759015629 64-65 3.299316282686739E-5 6.598632565373478E-4 0.0 0.41470756015230964 0.0013527196759015629 66-67 3.299316282686739E-5 7.588427450179499E-4 0.0 0.44732130160666805 0.0013857128387284303 68-69 3.299316282686739E-5 7.588427450179499E-4 0.0 0.4814362319696489 0.0014187060015552976 70-71 3.299316282686739E-5 7.753393264313835E-4 0.0 0.5147263332619582 0.0014352025829687315 72-73 3.299316282686739E-5 7.918359078448173E-4 0.0 0.5497320790212644 0.0016991478855836704 74-75 3.299316282686739E-5 7.918359078448173E-4 0.0 0.584523369222196 0.0018146239554777065 76-77 3.299316282686739E-5 8.248290706716847E-4 0.0 0.6235542808463802 0.0018476171183045738 78-79 3.299316282686739E-5 8.248290706716847E-4 0.0 0.6648617207056182 0.001880610281131441 80-81 3.299316282686739E-5 8.248290706716847E-4 0.0 0.7056412699596263 0.001946596606785176 82-83 3.299316282686739E-5 8.248290706716847E-4 0.0 0.7468662269117972 0.0020125829324389105 84-85 3.299316282686739E-5 8.413256520851184E-4 1.6496581413433694E-5 0.7871673753048156 0.0020125829324389105 86 3.299316282686739E-5 8.578222334985521E-4 3.299316282686739E-5 0.8182304381063112 0.0020125829324389105 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6030 0.0 34.049316 1 GGTATCA 3960 0.0 30.341436 1 TCAACGC 7610 0.0 26.684723 4 TATCAAC 7815 0.0 25.691462 2 CAACGCA 7910 0.0 25.556757 5 ATCAACG 8015 0.0 25.221952 3 GTACATG 8990 0.0 25.040329 1 AACGCAG 8365 0.0 24.104677 6 TACATGG 9540 0.0 23.448503 2 ACATGGG 9805 0.0 21.879728 3 GTACCTG 2995 0.0 21.365292 1 ACGCAGA 9690 0.0 20.706526 7 GTATAAT 690 0.0 20.01534 1 CATGGGG 7560 0.0 19.706305 4 CGCAGAG 10155 0.0 19.620123 8 TACCTGG 3185 0.0 18.998013 2 TATAGCG 150 9.81126E-4 18.33596 5 AGAGTAC 9295 0.0 17.690681 10-11 GTATATA 740 0.0 17.418756 1 AGTACAT 6820 0.0 17.341194 2 >>END_MODULE