FastQCFastQC Report
Mon 15 Aug 2016
SRR2921430_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921430_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3030931
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46540.15355017979624083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32230.1063369637909936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40750.036.0052031
GTATCAA65450.035.372741
TCAACGC78150.028.8036484
CAACGCA79850.028.2363645
ATCAACG82300.027.4084663
TATCAAC84050.027.0637992
AACGCAG86200.026.48676
GTACATG90050.024.8102591
TACATGG92500.023.6673772
ACGCAGA98350.022.973647
ACATGGG92250.022.9078643
GAGTACA71850.021.4149911
CGCAGAG106100.021.206078
GTACCTG28850.021.1351951
AGTACAT66250.020.5724952
CATGGGG68600.019.66044
TACCTGG30300.019.434352
TACGCCG1507.761437E-418.9867255
CCTATAC5050.018.8074363
AGAGTAC94650.018.63050710-11
Produced by FastQC (version 0.11.5)