##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921430_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3030931
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.922200142464476	33.0	31.0	34.0	30.0	34.0
2	32.0446951778183	34.0	31.0	34.0	30.0	34.0
3	32.167726022136435	34.0	31.0	34.0	30.0	34.0
4	35.70301468426698	37.0	35.0	37.0	35.0	37.0
5	35.50107277268931	37.0	35.0	37.0	33.0	37.0
6	35.5368812421002	37.0	35.0	37.0	33.0	37.0
7	35.4704168455171	37.0	35.0	37.0	33.0	37.0
8	35.42689589436381	37.0	35.0	37.0	33.0	37.0
9	37.05395602869218	39.0	37.0	39.0	34.0	39.0
10-11	36.96434313417231	39.0	37.0	39.0	33.0	39.0
12-13	36.84908020011014	39.0	37.0	39.0	33.0	39.0
14-15	38.147040298838874	40.0	38.0	41.0	33.0	41.0
16-17	38.051456796608036	40.0	38.0	41.0	33.0	41.0
18-19	37.96173865389875	40.0	38.0	41.0	33.0	41.0
20-21	37.85506977888972	40.0	38.0	41.0	33.0	41.0
22-23	37.821404710301884	40.0	38.0	41.0	33.0	41.0
24-25	37.872521677332806	40.0	38.0	41.0	33.0	41.0
26-27	37.76540195075374	40.0	38.0	41.0	33.0	41.0
28-29	37.63723456588091	40.0	38.0	41.0	32.5	41.0
30-31	37.498616431716854	40.0	38.0	41.0	32.0	41.0
32-33	37.33570477190012	40.0	37.0	41.0	32.0	41.0
34-35	37.18824941907289	40.0	37.0	41.0	31.0	41.0
36-37	37.089559610561906	40.0	37.0	41.0	31.0	41.0
38-39	37.05865953398477	40.0	37.0	41.0	31.0	41.0
40-41	36.90374195255517	40.0	37.0	41.0	31.0	41.0
42-43	36.67568133355725	40.0	36.0	41.0	30.0	41.0
44-45	36.47090662901927	39.0	36.0	41.0	30.0	41.0
46-47	36.19859986914911	39.0	35.5	40.5	30.0	41.0
48-49	35.87507072909281	39.0	35.0	40.0	29.0	41.0
50-51	35.60671852971909	39.0	35.0	40.0	28.0	41.0
52-53	35.30881122005087	38.0	35.0	40.0	27.5	41.0
54-55	34.99548752512017	38.0	34.0	40.0	27.0	41.0
56-57	34.62915421037299	38.0	34.0	40.0	26.0	41.0
58-59	34.25230712939357	37.0	33.5	40.0	26.0	41.0
60-61	33.84868939609645	37.0	33.0	39.5	25.0	41.0
62-63	33.4215025020365	36.0	33.0	39.0	23.5	40.0
64-65	32.920466846655366	35.5	32.0	39.0	22.5	40.0
66-67	32.40603662702978	35.0	31.5	38.0	21.5	40.0
68-69	31.920353845072682	35.0	31.0	38.0	20.0	40.0
70-71	31.380437231992413	35.0	30.5	37.0	18.0	39.0
72-73	30.842536831092495	34.0	30.0	36.0	17.5	39.0
74-75	30.325136896880856	34.0	29.5	36.0	13.0	38.5
76-77	29.001337542821002	32.5	28.0	34.5	11.5	36.0
78-79	29.951927477068928	34.0	30.0	35.0	11.0	37.0
80-81	29.98873349475788	34.0	30.0	35.0	9.5	37.0
82-83	29.657897523896125	34.0	30.0	35.0	7.0	36.0
84-85	29.176758890255172	34.0	29.0	35.0	3.5	36.0
86-87	28.661768446724786	33.5	29.0	35.0	2.0	35.0
88-89	28.11904098113748	33.0	27.5	35.0	2.0	35.0
90-91	27.658203205549714	33.0	27.0	35.0	2.0	35.0
92-93	27.23259635405755	33.0	26.0	34.0	2.0	35.0
94-95	26.75886188105239	32.5	25.0	34.0	2.0	35.0
96-97	26.00833621748565	32.0	24.5	34.0	2.0	35.0
98-99	25.009303082122294	32.0	20.5	34.0	2.0	35.0
100-101	21.395036046680048	27.5	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	21176.0
3	10887.0
4	6238.0
5	4682.0
6	3935.0
7	4064.0
8	4760.0
9	5539.0
10	6706.0
11	7899.0
12	9247.0
13	10933.0
14	12266.0
15	13684.0
16	15638.0
17	18742.0
18	18761.0
19	16352.0
20	16226.0
21	16990.0
22	18041.0
23	20496.0
24	23307.0
25	27708.0
26	32792.0
27	39211.0
28	48599.0
29	59077.0
30	72715.0
31	91430.0
32	118514.0
33	159750.0
34	229842.0
35	349290.0
36	543551.0
37	672130.0
38	291338.0
39	8415.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.59914194711542	17.99309493161626	13.880074498743737	24.527688622524582
2	16.0461587545213	19.2851305423977	38.496422386388865	26.172288316692132
3	19.75756559910095	22.441811586408416	28.672167160822475	29.128455653668155
4	14.008814790359855	15.309706173654671	34.98283261802575	35.69864641795972
5	15.347906490284288	35.781306965058405	33.30264952992743	15.568137014729874
6	34.871754408148185	33.72378212825187	16.418030678842772	14.986432784757179
7	30.725302242285128	30.763965746314586	19.584269930029294	18.926462081370993
8	27.687756976534118	33.91110977532213	19.068736361603655	19.332396886540103
9	26.689318493513714	16.225469006085877	19.370085916888776	37.71512658351163
10-11	26.36892559417904	25.168686824486937	26.391894379912962	22.07049320142106
12-13	26.36479133112089	22.21496043463285	27.05104677071902	24.36920146352724
14-15	23.66571529030735	23.831251475348676	24.628261655460896	27.874771578883077
16-17	23.90217844761349	26.921191842274112	25.321912138630644	23.854717571481757
18-19	23.87568063991498	26.272820363871602	26.75878014144603	23.092718854767387
20-21	24.473400217814596	25.414210752120393	26.694597538035048	23.417791492029966
22-23	24.34195043491492	25.046646948367034	26.562239555225926	24.049163061492116
24-25	24.014072943989582	25.247567804840216	26.726283054627544	24.012076196542655
26-27	23.96175106379503	25.523259775867558	26.657534477123022	23.85745468321439
28-29	24.022808840983444	25.58072765799675	26.588275438634412	23.808188062385394
30-31	24.20757195275374	25.52218970101718	26.392442630647643	23.877795715581442
32-33	24.02604215815213	25.472172491683654	26.339062115404033	24.162723234760183
34-35	24.172402796500432	25.46983526832681	26.48914868810725	23.868613247065504
36-37	24.22830709562335	25.413223515459926	26.395159891948555	23.96330949696817
38-39	24.163297240628992	25.55501071297638	26.40592759394107	23.875764452453556
40-41	24.30851019946837	25.46190894975918	26.329659885721075	23.899920965051372
42-43	24.30246645959413	25.395123831092686	26.18266595662827	24.119743752684915
44-45	24.22251150389623	25.47029445684616	26.233354616985018	24.0738394222726
46-47	24.27562650826063	25.466641915723038	26.190211001794445	24.06752057422189
48-49	24.30031888796689	25.6029259166998	26.023633673117562	24.07312152221575
50-51	24.26179814690226	25.768920737688774	25.928982294242044	24.040298821166928
52-53	24.32396674145841	25.65911562079482	25.88151120779066	24.135406429956113
54-55	24.349070699615265	25.516418676709772	25.967998894021925	24.16651172965303
56-57	24.316278606311855	25.711824335415333	25.901805830531426	24.07009122774139
58-59	24.321193049890503	25.61741404126679	25.971881173418677	24.08951173542403
60-61	24.358839840620625	25.61547349902137	25.958077786532467	24.06760887382554
62-63	24.224896786271895	25.698292038217662	25.927017916447987	24.149793259062456
64-65	24.30272436553114	25.530945741017657	25.930553227545627	24.235776665905572
66-67	24.289176390402815	25.61321630468596	25.85265909561621	24.244948209295014
68-69	24.252987990265705	25.668927360725657	25.896388826392926	24.181695822615712
70-71	24.187525325385835	25.518271234717325	25.98326841675257	24.310935023144268
72-73	24.350704321575005	25.55117482640728	25.790734262791602	24.307386589226113
74-75	24.26483149003075	25.723111629220462	25.69818912418191	24.31386775656687
76-77	24.32873994835048	25.58532645335012	25.7770015677545	24.308932030544902
78-79	24.26480032304641	25.55449311528801	25.881303556977375	24.299403004688198
80-81	24.24182267184497	25.668893602941967	25.848847215999616	24.240436509213442
82-83	24.3540756073445	25.603697729033858	25.753413445735358	24.288813217886286
84-85	24.348245038712683	25.6430018108544	25.672024980715847	24.33672816971707
86-87	24.31968053285925	25.61977028306826	25.832914642166877	24.22763454190562
88-89	24.23437462166104	25.51746818967482	25.793703311935722	24.45445387672842
90-91	24.258117225820115	25.583454771125965	25.79191701682928	24.366510986224643
92-93	24.33701325998237	25.595821160483233	25.87466683347343	24.192498746060963
94-95	24.325679385484445	25.562547019150816	25.75341177557512	24.358361819789618
96-97	24.27803539576568	25.452807515479627	25.850909244486033	24.418247844268663
98-99	24.19667747469863	25.534177616996413	25.776026098980193	24.49311880932477
100-101	24.682563827091407	25.061768152973844	25.913521946171958	24.342146073762787
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	353.0
1	299.5
2	430.5
3	605.0
4	477.0
5	410.5
6	769.0
7	1665.0
8	2468.5
9	2342.5
10	1582.5
11	1065.0
12	964.0
13	991.0
14	1048.0
15	1088.5
16	1142.5
17	1258.0
18	1376.5
19	1431.0
20	1487.5
21	1580.5
22	1714.0
23	1904.0
24	2210.5
25	2621.0
26	3285.5
27	4364.5
28	5877.0
29	8018.5
30	11056.5
31	14865.0
32	19730.0
33	25303.0
34	30968.5
35	37542.5
36	45160.0
37	52987.0
38	60167.0
39	67357.0
40	75096.0
41	83232.5
42	90749.0
43	98719.5
44	107074.0
45	114238.5
46	121396.5
47	127893.0
48	134140.0
49	136915.5
50	134161.0
51	132229.5
52	131661.0
53	129654.0
54	127236.5
55	122450.0
56	113259.5
57	103048.0
58	94152.5
59	84823.5
60	74156.5
61	63415.0
62	52206.0
63	39645.0
64	29092.5
65	21979.0
66	16944.0
67	13167.5
68	10086.5
69	7634.5
70	5656.0
71	4241.0
72	3145.5
73	2401.0
74	1796.5
75	1296.0
76	931.5
77	547.0
78	308.5
79	177.5
80	90.0
81	38.5
82	21.5
83	14.5
84	9.0
85	5.0
86	3.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31910987086146136
2	0.0
3	0.005707817169048058
4	0.06370979741868092
5	5.278906052298782E-4
6	0.063940749558469
7	0.07373971891804862
8	0.10472029881247709
9	0.0914570473560764
10-11	0.0959606140819438
12-13	0.0958121448492229
14-15	0.06552442137415863
16-17	0.0352037047362675
18-19	0.038057613320791536
20-21	0.0274173183091268
22-23	0.04150539883619917
24-25	0.033108638896761425
26-27	0.020604230185378686
28-29	0.02317769688587434
30-31	0.00290339832876433
32-33	0.00504795391251071
34-35	0.007077033426363054
36-37	0.011135192454067744
38-39	0.016298622436472492
40-41	0.020653719929618983
42-43	0.003596254748128545
44-45	0.01438501899251418
46-47	0.024530416561775903
48-49	0.0028374120031105956
50-51	0.00732448214756456
52-53	0.0023260179792941506
54-55	0.004272614586079327
56-57	0.0036127513295419788
58-59	0.0014352025829687315
60-61	0.005773803494701793
62-63	0.014764440365023156
64-65	0.006829584705161549
66-67	0.03921237401973189
68-69	0.06092187515981064
70-71	0.01237243606007527
72-73	0.03279520384990618
74-75	0.05082596733478922
76-77	0.31053164852647586
78-79	0.07469652064002777
80-81	0.032448775640224074
82-83	0.028209154216971618
84-85	0.019581442137745795
86-87	0.12009511268979728
88-89	0.5314538668151799
90-91	0.132104623958777
92-93	0.04874739807669657
94-95	0.008281283869543715
96-97	0.005707817169048058
98-99	0.010376349709049793
100-101	0.008017338566928775
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3030931.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.59798519914439
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4369671255282	43.61856297890899
2	14.685300132943146	17.210579996703913
3	4.886968978447688	8.590996076032662
4	2.0226688846883696	4.740972854709558
5	1.0974415361742829	3.2153931446833455
6	0.6653783924241377	2.3393899914660063
7	0.4327024667688565	1.7748844920348301
8	0.3152902587982826	1.4780299134796935
9	0.22668164296780635	1.1954778803590722
>10	1.1606698410567646	11.944862672809927
>50	0.05554464562890023	2.172070794500841
>100	0.013878782144604269	1.2352120957072186
>500	3.3820828610188003E-4	0.12911638176021897
>1k	1.691041428324909E-4	0.354450726843716
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4654	0.15355017979624083	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3223	0.1063369637909936	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.897948848060216E-5
2	0.0	0.0	0.0	0.0	9.897948848060216E-5
3	0.0	0.0	0.0	0.0	9.897948848060216E-5
4	0.0	0.0	0.0	0.0	9.897948848060216E-5
5	0.0	0.0	0.0	0.0	9.897948848060216E-5
6	0.0	0.0	0.0	0.0	1.3197265130746955E-4
7	0.0	0.0	0.0	0.0	1.3197265130746955E-4
8	0.0	0.0	0.0	0.0	1.3197265130746955E-4
9	0.0	0.0	0.0	0.0	1.6496581413433695E-4
10-11	0.0	0.0	0.0	0.0	1.8146239554777064E-4
12-13	0.0	0.0	0.0	0.0	4.1241453533584233E-4
14-15	0.0	1.6496581413433694E-5	0.0	0.0	4.7840086098957713E-4
16-17	0.0	3.299316282686739E-5	0.0	0.0	4.948974424030108E-4
18-19	0.0	3.299316282686739E-5	0.0	0.0	5.278906052298782E-4
20-21	0.0	3.299316282686739E-5	0.0	3.299316282686739E-5	6.433666751239141E-4
22-23	0.0	4.948974424030108E-5	0.0	8.248290706716846E-5	6.928564193642152E-4
24-25	0.0	6.598632565373477E-5	0.0	2.639453026149391E-4	7.588427450179499E-4
26-27	0.0	6.598632565373477E-5	0.0	4.1241453533584233E-4	8.578222334985521E-4
28-29	0.0	8.248290706716846E-5	0.0	0.0013362230944881292	8.578222334985521E-4
30-31	0.0	9.897948848060216E-5	0.0	0.00504795391251071	8.908153963254195E-4
32-33	0.0	9.897948848060216E-5	0.0	0.013246754874987256	9.238085591522869E-4
34-35	0.0	1.3197265130746955E-4	0.0	0.022633309699231028	9.568017219791543E-4
36-37	0.0	1.9795897696120432E-4	0.0	0.03655642441216907	9.568017219791543E-4
38-39	0.0	3.134350468552402E-4	0.0	0.05780402127267166	9.568017219791543E-4
40-41	0.0	3.9591795392240865E-4	0.0	0.0811301873912669	9.568017219791543E-4
42-43	0.0	4.2891111674927607E-4	0.0	0.10495125095226517	9.897948848060216E-4
44-45	0.0	4.2891111674927607E-4	0.0	0.12928370853707985	0.0010227880476328891
46-47	0.0	4.2891111674927607E-4	0.0	0.15620612940380363	0.0010392846290463228
48-49	0.0	4.2891111674927607E-4	0.0	0.1835409648058633	0.0011217675361134911
50-51	0.0	4.2891111674927607E-4	0.0	0.21006746771866466	0.0011217675361134911
52-53	0.0	4.6190427957614345E-4	0.0	0.2351587680484973	0.0012702367688343943
54-55	0.0	4.948974424030108E-4	0.0	0.2652815257094272	0.0014187060015552976
56-57	0.0	4.948974424030108E-4	3.299316282686739E-5	0.294826903020887	0.0014352025829687315
58-59	0.0	4.948974424030108E-4	3.299316282686739E-5	0.3247846948676826	0.0014681957457955987
60-61	0.0	4.948974424030108E-4	3.299316282686739E-5	0.35436306534196915	0.0014846923272090326
62-63	0.0	4.948974424030108E-4	3.299316282686739E-5	0.3821927981864318	0.0015176854900358998
64-65	0.0	4.948974424030108E-4	3.299316282686739E-5	0.4110783122413542	0.0015176854900358998
66-67	0.0	4.948974424030108E-4	3.299316282686739E-5	0.4434281083930977	0.0015176854900358998
68-69	0.0	4.948974424030108E-4	3.299316282686739E-5	0.4773945695233577	0.0015176854900358998
70-71	0.0	4.948974424030108E-4	3.299316282686739E-5	0.5100907938847833	0.0016001683971030682
72-73	0.0	4.948974424030108E-4	3.299316282686739E-5	0.5447996011786478	0.0016826513041702368
74-75	0.0	4.948974424030108E-4	3.299316282686739E-5	0.5790465041929361	0.0017156444669971043
76-77	0.0	4.948974424030108E-4	3.299316282686739E-5	0.6176485047003709	0.001732141048410538
78-79	0.0	5.113940238164446E-4	3.299316282686739E-5	0.6582300949774178	0.0018146239554777063
80-81	0.0	5.278906052298782E-4	3.299316282686739E-5	0.698481753626196	0.0018641136997180074
82-83	0.0	5.278906052298782E-4	3.299316282686739E-5	0.7389973575775892	0.001946596606785176
84-85	0.0	5.278906052298782E-4	3.299316282686739E-5	0.7785891529698301	0.001946596606785176
86-87	0.0	5.278906052298782E-4	3.299316282686739E-5	0.8195501646193859	0.0019795897696120433
88-89	0.0	5.278906052298782E-4	3.299316282686739E-5	0.8637115130631479	0.0020125829324389105
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4075	0.0	36.005203	1
GTATCAA	6545	0.0	35.37274	1
TCAACGC	7815	0.0	28.803648	4
CAACGCA	7985	0.0	28.236364	5
ATCAACG	8230	0.0	27.408466	3
TATCAAC	8405	0.0	27.063799	2
AACGCAG	8620	0.0	26.4867	6
GTACATG	9005	0.0	24.810259	1
TACATGG	9250	0.0	23.667377	2
ACGCAGA	9835	0.0	22.97364	7
ACATGGG	9225	0.0	22.907864	3
GAGTACA	7185	0.0	21.414991	1
CGCAGAG	10610	0.0	21.20607	8
GTACCTG	2885	0.0	21.135195	1
AGTACAT	6625	0.0	20.572495	2
CATGGGG	6860	0.0	19.6604	4
TACCTGG	3030	0.0	19.43435	2
TACGCCG	150	7.761437E-4	18.986725	5
CCTATAC	505	0.0	18.807436	3
AGAGTAC	9465	0.0	18.630507	10-11
>>END_MODULE