FastQCFastQC Report
Mon 15 Aug 2016
SRR2921429_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921429_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4374495
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57380.13116942641379176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA106200.034.631021
GTACCTG54050.033.042971
TACCTGG56800.031.1565592
TCAACGC130350.027.7860344
TATCAAC131600.027.6962992
CAACGCA132300.027.3764885
ATCAACG134800.026.8687653
AACGCAG139600.026.0031556
GTACATG140400.024.6215781
ACCTGGG70000.024.233393
GGTATCA67600.024.1726761
TACATGG145450.023.1362422
ACATGGG145050.022.4730683
ACGCAGA161200.022.3994967
CGCAGAG169950.021.0801288
CATGGGG112450.019.9370044
GTATAGG11600.019.8406121
AGAGTAC140700.019.19463510-11
GCAGAGT185900.018.9508919
GAGTACA107550.018.8315181
Produced by FastQC (version 0.11.5)