##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921429_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4374495
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.011996356150824	31.0	31.0	34.0	26.0	34.0
2	30.109926517232275	31.0	31.0	34.0	26.0	34.0
3	30.234864138603427	31.0	31.0	34.0	26.0	34.0
4	33.724988370086145	37.0	35.0	37.0	28.0	37.0
5	33.70042736361569	37.0	35.0	37.0	28.0	37.0
6	33.79180408252839	37.0	35.0	37.0	30.0	37.0
7	33.66670049914333	37.0	35.0	37.0	29.0	37.0
8	33.64068263879602	37.0	35.0	37.0	29.0	37.0
9	35.05093707959433	39.0	35.0	39.0	29.0	39.0
10-11	34.914222098779405	39.0	35.0	39.0	28.0	39.0
12-13	34.74069806914855	38.5	35.0	39.0	27.0	39.0
14-15	35.703878276235315	39.5	36.0	41.0	27.0	41.0
16-17	35.5483816989161	39.0	36.0	41.0	27.0	41.0
18-19	35.35824089409178	39.0	36.0	40.5	26.5	41.0
20-21	35.23415902864216	39.0	35.5	40.0	25.5	41.0
22-23	35.260975724055	39.0	35.5	41.0	25.5	41.0
24-25	35.11373301375359	39.0	35.0	41.0	25.0	41.0
26-27	34.824614841255965	39.0	35.0	40.0	24.0	41.0
28-29	34.57131954659909	39.0	34.0	40.0	22.5	41.0
30-31	34.28906559500011	38.0	34.0	40.0	20.0	41.0
32-33	33.98901233170915	38.0	34.0	40.0	18.0	41.0
34-35	33.548733282356025	38.0	33.0	40.0	14.5	41.0
36-37	33.15010052588927	38.0	33.0	40.0	9.0	41.0
38-39	32.897993139779565	38.0	32.0	40.0	9.0	41.0
40-41	32.60786250755801	38.0	31.5	40.0	8.5	41.0
42-43	32.286322992711156	37.5	31.0	40.0	7.5	41.0
44-45	31.943615891662922	37.0	30.5	40.0	4.0	41.0
46-47	31.619624093752535	37.0	30.0	40.0	2.0	41.0
48-49	31.375085238410378	37.0	30.0	40.0	2.0	41.0
50-51	30.65090404721002	36.0	29.0	38.5	2.0	39.5
52-53	31.185170745423186	36.5	30.5	39.0	2.0	40.0
54-55	31.597532286583938	37.0	31.0	39.5	2.0	40.5
56-57	31.434562846682873	36.5	31.0	40.0	2.0	41.0
58-59	31.09203542351746	36.0	30.0	39.0	2.0	41.0
60-61	30.62509661115169	35.5	29.5	39.0	2.0	40.0
62-63	30.119700445422843	35.0	29.0	39.0	2.0	40.0
64-65	29.561330736462153	35.0	28.0	38.0	2.0	40.0
66-67	29.060529501119557	34.0	27.5	37.5	2.0	40.0
68-69	28.532810072934133	34.0	26.0	37.0	2.0	39.0
70-71	27.997627611872915	34.0	26.0	36.0	2.0	39.0
72-73	27.428133075932195	33.5	25.5	36.0	2.0	39.0
74-75	26.81371015397206	33.0	24.0	35.0	2.0	38.0
76-77	26.210054760606653	32.5	22.0	35.0	2.0	37.0
78-79	25.625852927023576	32.0	20.0	35.0	2.0	36.5
80-81	25.02317776109014	31.5	18.0	34.5	2.0	36.0
82-83	24.367444356434284	31.0	10.5	34.0	2.0	35.5
84-85	23.720633467405953	31.0	4.5	34.0	2.0	35.0
86-87	23.067346745167157	30.0	2.0	34.0	2.0	35.0
88-89	22.387593882265268	30.0	2.0	34.0	2.0	35.0
90-91	21.622544659440692	29.0	2.0	34.0	2.0	35.0
92-93	20.778579813212726	29.0	2.0	34.0	2.0	35.0
94-95	19.855126591755162	27.0	2.0	33.0	2.0	34.5
96-97	18.95179569298856	25.5	2.0	33.0	2.0	34.0
98	18.326442709387027	25.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	171986.0
3	27973.0
4	21236.0
5	17754.0
6	17103.0
7	17741.0
8	20048.0
9	23528.0
10	25769.0
11	28243.0
12	31039.0
13	34815.0
14	30416.0
15	30997.0
16	34312.0
17	39946.0
18	43909.0
19	41766.0
20	43074.0
21	46812.0
22	50728.0
23	55564.0
24	61639.0
25	69192.0
26	77027.0
27	87239.0
28	99688.0
29	116151.0
30	136666.0
31	162097.0
32	200248.0
33	254326.0
34	335768.0
35	455942.0
36	595994.0
37	601413.0
38	254509.0
39	11837.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.72705625065887	17.230248121911913	13.751309419390639	24.291386208038578
2	15.88216391890393	18.624125040453123	39.36747900726041	26.126232033382546
3	20.321430866905875	22.642499881631792	27.428898448312893	29.60717080314944
4	13.82863460879999	14.90327357297099	34.803764712986265	36.46432710524275
5	15.699135080952772	35.51614583416731	32.42148282228363	16.363236262596295
6	34.84826927097606	33.08114646586876	16.84407586559326	15.226508397561913
7	31.26227522670577	30.073036169552957	19.241037655158408	19.423650948582864
8	28.02088618346999	33.46005552851204	18.720694968388806	19.798363319629168
9	26.934018417622646	16.81939239083641	19.019045199970826	37.227543991570116
10-11	26.698526469814922	25.031208203299155	25.851663865930476	22.41860146095545
12-13	26.901786403216725	22.018089797411417	26.618888263350872	24.461235536020986
14-15	23.901543007459026	23.53177542283608	24.338597180936564	28.22808438876833
16-17	24.322470571088076	26.600741331367118	24.76563468996402	24.311153407580786
18-19	24.282302368831612	25.91871220755564	26.306057243809626	23.49292817980312
20-21	24.86931828386351	25.022046730489027	26.308633178210222	23.800001807437237
22-23	24.564492480439533	24.731445086242257	26.232370979856878	24.471691453461332
24-25	24.360149676336075	24.999917075237924	26.31127210588291	24.32866114254309
26-27	24.375559257436535	25.12867925901064	26.272029680940417	24.22373180261241
28-29	24.36133874194334	25.29087256449818	26.14059053208015	24.20719816147833
30-31	24.523724485332185	25.171899032713053	26.029681581794755	24.274694900160007
32-33	24.370332935824187	25.241255275190284	25.993359348649058	24.395052440336467
34-35	24.560013586769426	25.19024919787569	26.133428042908484	24.1163091724464
36-37	24.623459455256892	25.143102543654184	25.988227005201033	24.245210995887895
38-39	24.545656858908895	25.224763350733824	25.961471701635475	24.268108088721807
40-41	24.697708533266113	25.159190459457744	25.929576074113896	24.213524933162248
42-43	24.570155604102236	25.092191388529695	25.89785409037198	24.439798916996093
44-45	24.563982378911316	25.207573547777933	25.839376575055418	24.389067498255333
46-47	24.716753772662482	25.216757850999176	25.77438710354116	24.292101272797183
48-49	24.707453914586537	25.288870233942674	25.559746026232254	24.443929825238534
50-51	24.463860133509925	25.544480790140522	25.580739681381313	24.41091939496824
52-53	24.653861796306963	25.52901701246087	25.469879930912064	24.3472412603201
54-55	24.55712094618804	25.258471840383418	25.680392785927737	24.50401442750081
56-57	24.513322938940025	25.44745105653991	25.64197251422144	24.397253490298628
58-59	24.693532633087024	25.32961962861498	25.65643927519579	24.32040846310221
60-61	24.67294308656526	25.26559821162588	25.67861631238828	24.382842389420578
62-63	24.492078235922968	25.446644832331238	25.70003695229936	24.361239979446434
64-65	24.540654060717166	25.294429835035626	25.683149159778306	24.481766944468898
66-67	24.50028503246749	25.380799123210473	25.648496282737494	24.470419561584546
68-69	24.463376058727455	25.37585075928489	25.647829461506067	24.51294372048159
70-71	24.50934276241673	25.21934906710183	25.6773631946494	24.59394497583204
72-73	24.62108986545207	25.26248977109411	25.576472833277474	24.53994753017635
74-75	24.436242828049988	25.46577783083956	25.562494403640446	24.53548493747001
76-77	24.559238983299544	25.27320305742184	25.514861268117766	24.652696691160852
78-79	24.596163405978878	25.19372974387882	25.632806104935174	24.57730074520713
80-81	24.57193698464223	25.34296530366824	25.50039463525657	24.584703076432955
82-83	24.70017807201789	25.24429211894678	25.486333650099734	24.56919615893559
84-85	24.65938956659724	25.299791820482586	25.433852726077262	24.606965886842907
86-87	24.6118411159489	25.303351542958687	25.554329846375655	24.53047749471676
88-89	24.53730771580174	25.17215041856676	25.503591356494322	24.78695050913719
90-91	24.627241005412337	25.152873119185642	25.506130136023064	24.713755739378957
92-93	24.667329275942095	25.263138077119823	25.56072678548633	24.50880586145175
94-95	24.716069920684532	25.062125760104152	25.535974962558072	24.68582935665324
96-97	24.665240136582277	25.10002988296966	25.534888510577918	24.699841469870147
98	24.66017643159826	25.17480494511739	25.37303392507385	24.7919846982105
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	581.0
1	446.0
2	566.5
3	788.0
4	592.5
5	470.0
6	718.0
7	1507.0
8	2228.5
9	2210.5
10	1859.5
11	1679.5
12	1815.5
13	2134.0
14	2534.0
15	2926.0
16	3211.5
17	3461.0
18	3677.5
19	3714.0
20	3653.5
21	3734.0
22	3941.0
23	4297.5
24	4943.0
25	6013.0
26	7507.0
27	9543.5
28	12192.0
29	15614.0
30	19930.5
31	25224.5
32	31498.0
33	38599.5
34	46858.0
35	55954.5
36	65338.0
37	75233.5
38	86007.5
39	96726.5
40	106971.0
41	117911.5
42	130114.0
43	143277.5
44	155117.0
45	166241.0
46	178999.5
47	191620.0
48	197690.5
49	197679.5
50	197503.0
51	197257.0
52	197285.0
53	198166.5
54	195875.0
55	188518.5
56	177035.5
57	165034.5
58	151835.0
59	134796.0
60	116373.0
61	97452.5
62	78964.5
63	60549.5
64	44505.0
65	33318.0
66	25526.0
67	19356.0
68	14622.0
69	11130.5
70	8374.5
71	6286.5
72	4818.0
73	3596.0
74	2659.5
75	2038.0
76	1431.0
77	898.5
78	558.5
79	347.5
80	211.5
81	128.0
82	78.0
83	47.0
84	31.5
85	24.5
86	17.5
87	14.0
88	11.5
89	10.5
90	8.5
91	6.5
92	5.5
93	5.5
94	8.0
95	7.0
96	4.0
97	3.5
98	3.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4678482887739042
2	0.049148530287496044
3	0.0581552842099488
4	0.05536639086340252
5	0.06565329255148308
6	0.06558471320689588
7	0.04267921211476982
8	0.020253766434754183
9	0.012161403773464138
10-11	0.014744559086248813
12-13	0.011029844587775275
14-15	0.015236044389123774
16-17	0.013887317278908767
18-19	0.05541211042646066
20-21	0.08379252919479849
22-23	0.10041159036643087
24-25	0.07000808093277053
26-27	0.08558702204483032
28-29	0.08708433773498427
30-31	0.057709518470131976
32-33	0.03268948758656714
34-35	0.39581711717581114
36-37	0.619225762059392
38-39	0.5671283199546462
40-41	0.5622477565981902
42-43	0.533924487283675
44-45	0.5180940885747956
46-47	0.5081843732819445
48-49	0.49747456563557624
50-51	0.5134078333613366
52-53	0.597177502774606
54-55	0.5656995836090795
56-57	0.5209744210474581
58-59	0.4980803498460965
60-61	0.51172763941895
62-63	0.5243805284952892
64-65	0.5440170808287585
66-67	0.571277370302172
68-69	0.5227460541159609
70-71	0.4706829016835086
72-73	0.4526579639478385
74-75	0.4339929523293546
76-77	0.4476859614652663
78-79	0.441879576956883
80-81	0.4390792537195722
82-83	0.45895583375909677
84-85	0.42608346792029705
86-87	0.428392305854733
88-89	0.4542924383271669
90-91	0.43816486245840947
92-93	0.43403867189241274
94-95	0.44387980784067643
96-97	0.4382563015845257
98	0.4326442252191396
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4374495.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.89891430302304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.7619961970308	44.44436239540866
2	13.036760975236685	15.096286129884476
3	4.3014135757073895	7.47141528005226
4	1.9530147263529556	4.523097290946071
5	1.0216063105976647	2.957494811436086
6	0.640751096666369	2.225927570127248
7	0.428797134785244	1.7378821992218862
8	0.3101767379004188	1.4367117093190067
9	0.2314146794528636	1.2058792824692606
>10	1.2128304250696245	12.728797035506222
>50	0.07164919746567183	2.827183423161762
>100	0.02867665420816329	2.696625000310012
>500	5.949716126251496E-4	0.21626583278269926
>1k	2.776531743350559E-4	0.30029626803774623
>5k	3.9664739190649735E-5	0.1317757713367132
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5738	0.13116942641379176	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.857934458720378E-5
2	0.0	0.0	0.0	2.2859781529067928E-5	6.857934458720378E-5
3	0.0	0.0	0.0	2.2859781529067928E-5	6.857934458720378E-5
4	0.0	0.0	0.0	2.2859781529067928E-5	6.857934458720378E-5
5	0.0	0.0	0.0	2.2859781529067928E-5	6.857934458720378E-5
6	2.2859781529067928E-5	0.0	0.0	2.2859781529067928E-5	6.857934458720378E-5
7	2.2859781529067928E-5	0.0	0.0	2.2859781529067928E-5	6.857934458720378E-5
8	2.2859781529067928E-5	0.0	0.0	2.2859781529067928E-5	6.857934458720378E-5
9	2.2859781529067928E-5	0.0	0.0	2.2859781529067928E-5	6.857934458720378E-5
10-11	2.2859781529067928E-5	0.0	0.0	4.5719563058135856E-5	6.857934458720378E-5
12-13	2.2859781529067928E-5	4.5719563058135856E-5	0.0	4.5719563058135856E-5	1.600184707034755E-4
14-15	2.2859781529067928E-5	4.5719563058135856E-5	0.0	4.5719563058135856E-5	1.7144836146800946E-4
16-17	2.2859781529067928E-5	4.5719563058135856E-5	0.0	4.5719563058135856E-5	1.8287825223254342E-4
18-19	2.2859781529067928E-5	4.5719563058135856E-5	0.0	4.5719563058135856E-5	2.0573803376161133E-4
20-21	2.2859781529067928E-5	4.5719563058135856E-5	0.0	5.714945382266982E-5	2.514575968197472E-4
22-23	2.2859781529067928E-5	4.5719563058135856E-5	0.0	6.857934458720378E-5	2.9717715987788305E-4
24-25	2.2859781529067928E-5	4.5719563058135856E-5	0.0	1.3715868917440756E-4	2.9717715987788305E-4
26-27	2.2859781529067928E-5	4.5719563058135856E-5	0.0	1.8287825223254342E-4	3.20036941406951E-4
28-29	3.428967229360189E-5	6.857934458720378E-5	2.2859781529067928E-5	3.20036941406951E-4	3.20036941406951E-4
30-31	4.5719563058135856E-5	6.857934458720378E-5	2.2859781529067928E-5	0.0011429890764533962	3.20036941406951E-4
32-33	5.714945382266982E-5	6.857934458720378E-5	2.2859781529067928E-5	0.0026174449850782776	3.20036941406951E-4
34-35	6.857934458720378E-5	6.857934458720378E-5	2.2859781529067928E-5	0.004663395431929857	3.6575650446508685E-4
36-37	6.857934458720378E-5	1.1429890764533963E-4	2.2859781529067928E-5	0.00755515779535695	3.6575650446508685E-4
38-39	6.857934458720378E-5	1.3715868917440756E-4	2.2859781529067928E-5	0.01236714180722575	3.8861628599415473E-4
40-41	6.857934458720378E-5	1.3715868917440756E-4	2.2859781529067928E-5	0.01776205024808578	3.8861628599415473E-4
42-43	6.857934458720378E-5	1.3715868917440756E-4	2.2859781529067928E-5	0.023122669016652206	3.8861628599415473E-4
44-45	6.857934458720378E-5	1.3715868917440756E-4	2.2859781529067928E-5	0.02898620297885813	4.1147606752322267E-4
46-47	6.857934458720378E-5	1.3715868917440756E-4	2.2859781529067928E-5	0.034792587487241386	4.5719563058135854E-4
48-49	6.857934458720378E-5	1.3715868917440756E-4	4.5719563058135856E-5	0.04062183177715371	5.029151936394945E-4
50-51	6.857934458720378E-5	1.3715868917440756E-4	4.5719563058135856E-5	0.047411186891286886	5.257749751685623E-4
52-53	6.857934458720378E-5	1.3715868917440756E-4	4.5719563058135856E-5	0.05398337408089391	6.286439920493679E-4
54-55	6.857934458720378E-5	1.8287825223254342E-4	4.5719563058135856E-5	0.06096703733802416	6.629336643429698E-4
56-57	6.857934458720378E-5	1.8287825223254342E-4	4.5719563058135856E-5	0.0682478777550323	6.629336643429698E-4
58-59	6.857934458720378E-5	1.8287825223254342E-4	4.5719563058135856E-5	0.07607735292873806	6.629336643429698E-4
60-61	6.857934458720378E-5	1.8287825223254342E-4	4.5719563058135856E-5	0.08426115471614437	6.629336643429698E-4
62-63	6.857934458720378E-5	2.0573803376161136E-4	4.5719563058135856E-5	0.09367938470612036	6.857934458720378E-4
64-65	6.857934458720378E-5	2.2859781529067927E-4	4.5719563058135856E-5	0.10291473644386381	6.972233366365718E-4
66-67	8.000923535173774E-5	2.2859781529067927E-4	4.5719563058135856E-5	0.11181862134943577	7.315130089301737E-4
68-69	9.143912611627171E-5	2.514575968197472E-4	4.5719563058135856E-5	0.12147687904546697	7.315130089301737E-4
70-71	9.143912611627171E-5	2.8574726911334905E-4	4.5719563058135856E-5	0.13134087477525977	7.429428996947076E-4
72-73	9.143912611627171E-5	3.20036941406951E-4	4.5719563058135856E-5	0.14150204766493046	7.772325719883095E-4
74-75	9.143912611627171E-5	3.428967229360189E-4	4.5719563058135856E-5	0.151274604268607	8.000923535173774E-4
76-77	1.0286901688080568E-4	3.428967229360189E-4	4.5719563058135856E-5	0.16134433803216142	8.229521350464454E-4
78-79	1.1429890764533963E-4	3.428967229360189E-4	4.5719563058135856E-5	0.1716655293925356	8.572418073400473E-4
80-81	1.1429890764533963E-4	3.6575650446508685E-4	4.5719563058135856E-5	0.18284396256024982	8.915314796336491E-4
82-83	1.1429890764533963E-4	3.6575650446508685E-4	4.5719563058135856E-5	0.1943881522324291	0.0010286901688080568
84-85	1.1429890764533963E-4	3.6575650446508685E-4	4.5719563058135856E-5	0.2061266500476055	0.0010286901688080568
86	1.1429890764533963E-4	3.6575650446508685E-4	6.857934458720378E-5	0.21506482462547105	0.0010286901688080568
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10620	0.0	34.63102	1
GTACCTG	5405	0.0	33.04297	1
TACCTGG	5680	0.0	31.156559	2
TCAACGC	13035	0.0	27.786034	4
TATCAAC	13160	0.0	27.696299	2
CAACGCA	13230	0.0	27.376488	5
ATCAACG	13480	0.0	26.868765	3
AACGCAG	13960	0.0	26.003155	6
GTACATG	14040	0.0	24.621578	1
ACCTGGG	7000	0.0	24.23339	3
GGTATCA	6760	0.0	24.172676	1
TACATGG	14545	0.0	23.136242	2
ACATGGG	14505	0.0	22.473068	3
ACGCAGA	16120	0.0	22.399496	7
CGCAGAG	16995	0.0	21.080128	8
CATGGGG	11245	0.0	19.937004	4
GTATAGG	1160	0.0	19.840612	1
AGAGTAC	14070	0.0	19.194635	10-11
GCAGAGT	18590	0.0	18.950891	9
GAGTACA	10755	0.0	18.831518	1
>>END_MODULE