FastQCFastQC Report
Mon 15 Aug 2016
SRR2921429_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921429_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4374495
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61710.14106771181587818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG53700.035.2119561
GTATCAA113950.033.9402961
TACCTGG56200.033.046422
TCAACGC138950.027.4169834
TATCAAC142100.027.108832
CAACGCA142550.026.645135
ATCAACG143200.026.6364543
GGTATCA74950.025.9911561
AACGCAG151100.025.3276846
ACCTGGG73400.024.7860073
GTACATG136400.024.1289041
TACATGG139400.022.6931842
ACGCAGA174550.021.8446677
ACATGGG141800.020.9031753
CGCAGAG182850.020.8271168
TATCACG5150.020.2908132
AGAGTAC148400.020.04516410-11
GCAGAGT199950.018.7601179
GAGTACA107100.018.6336731
CATGGGG112850.018.4785044
Produced by FastQC (version 0.11.5)