##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921429_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4374495
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72510472637413	33.0	31.0	34.0	30.0	34.0
2	31.846508682716518	34.0	31.0	34.0	30.0	34.0
3	31.971272341150236	34.0	31.0	34.0	30.0	34.0
4	35.536683434316416	37.0	35.0	37.0	33.0	37.0
5	35.30925032489464	37.0	35.0	37.0	33.0	37.0
6	35.34122407272154	37.0	35.0	37.0	33.0	37.0
7	35.26555545268654	37.0	35.0	37.0	33.0	37.0
8	35.2149381814358	37.0	35.0	37.0	33.0	37.0
9	36.8000105154995	39.0	37.0	39.0	33.0	39.0
10-11	36.69363915149063	39.0	37.0	39.0	33.0	39.0
12-13	36.5624304062526	39.0	37.0	39.0	32.0	39.0
14-15	37.79753708713805	40.0	38.0	41.0	33.0	41.0
16-17	37.68201426678965	40.0	38.0	41.0	32.0	41.0
18-19	37.57101425421678	40.0	38.0	41.0	32.0	41.0
20-21	37.44545004623391	40.0	38.0	41.0	32.0	41.0
22-23	37.40595600177849	40.0	37.5	41.0	32.0	41.0
24-25	37.45386564620602	40.0	38.0	41.0	32.0	41.0
26-27	37.32508861022815	40.0	37.0	41.0	32.0	41.0
28-29	37.17280303212142	40.0	37.0	41.0	31.0	41.0
30-31	37.00987862598997	40.0	37.0	41.0	31.0	41.0
32-33	36.81995418899781	40.0	36.5	41.0	30.0	41.0
34-35	36.64706817586944	39.5	36.0	41.0	30.0	41.0
36-37	36.522109180602556	39.0	36.0	41.0	30.0	41.0
38-39	36.46657019838861	39.0	36.0	41.0	30.0	41.0
40-41	36.27770531227033	39.0	36.0	41.0	30.0	41.0
42-43	36.00654658423429	39.0	35.0	40.0	29.0	41.0
44-45	35.75873352238372	39.0	35.0	40.0	28.5	41.0
46-47	35.43582264924294	38.5	35.0	40.0	27.0	41.0
48-49	35.046551659105795	38.0	34.5	40.0	26.0	41.0
50-51	34.72927515061738	38.0	34.0	40.0	25.5	41.0
52-53	34.38098214765361	38.0	34.0	40.0	24.0	41.0
54-55	34.019762738327515	38.0	33.0	40.0	23.5	41.0
56-57	33.597863524818294	37.0	33.0	40.0	22.5	41.0
58-59	33.1691803282436	36.5	32.0	39.0	21.5	41.0
60-61	32.695382552728944	36.0	31.5	39.0	19.5	40.0
62-63	32.22067930126792	35.5	31.0	39.0	18.0	40.0
64-65	31.66166048881071	35.0	31.0	38.0	14.0	40.0
66-67	31.092010277757776	35.0	30.0	38.0	9.5	40.0
68-69	30.551088868543683	34.0	29.0	37.0	7.5	39.0
70-71	29.95523586151087	34.0	29.0	36.5	7.0	39.0
72-73	29.364927037292304	34.0	28.0	36.0	2.0	39.0
74-75	28.806073158158824	33.5	27.5	35.0	2.0	37.5
76-77	27.647804489432495	31.5	26.5	34.0	2.0	36.0
78-79	28.56907071559117	33.0	28.0	35.0	2.0	36.5
80-81	28.56902373874013	34.0	28.5	35.0	2.0	36.0
82-83	28.181537068850233	33.0	27.5	35.0	2.0	36.0
84-85	27.627336984040443	33.0	26.5	35.0	2.0	35.5
86-87	27.03717514821711	33.0	25.5	35.0	2.0	35.0
88-89	26.433196288943066	32.0	25.0	34.0	2.0	35.0
90-91	25.872108437659662	32.0	23.5	34.0	2.0	35.0
92-93	25.341185325391848	31.5	21.0	34.0	2.0	35.0
94-95	24.74380859962121	31.0	18.5	34.0	2.0	35.0
96-97	23.84989924551291	31.0	6.5	34.0	2.0	35.0
98-99	22.70441742418268	31.0	2.0	34.0	2.0	35.0
100-101	19.199852440110227	24.5	2.0	31.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36611.0
3	18294.0
4	11022.0
5	8274.0
6	7290.0
7	7425.0
8	8807.0
9	10569.0
10	12418.0
11	14799.0
12	17579.0
13	21079.0
14	23410.0
15	26544.0
16	30509.0
17	36356.0
18	36792.0
19	32719.0
20	32395.0
21	33982.0
22	36600.0
23	39537.0
24	44396.0
25	51032.0
26	60674.0
27	72626.0
28	87671.0
29	105206.0
30	127467.0
31	158078.0
32	198822.0
33	260907.0
34	359340.0
35	515179.0
36	736527.0
37	794143.0
38	291769.0
39	7647.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.965738243445216	17.81303614251181	14.165467022119168	24.055758591923805
2	16.0910687976555	19.04837015472643	38.835522728909275	26.025038318708788
3	20.342866731470362	22.906788751224212	27.70076877424995	29.049575743055474
4	14.150500171321315	15.313540879919083	34.74204133578475	35.79391761297486
5	15.734878235618325	35.55068233362053	32.4629732541497	16.251466176611444
6	34.86659895969147	33.305541449935724	16.651203857467667	15.176655732905134
7	31.341969376559607	30.114826463205844	19.155915459319576	19.38728870091497
8	28.08069260019584	33.59933839317322	18.63087295624887	19.689096050382073
9	26.84073483414979	16.80888500426724	19.0585766878465	37.29180347373647
10-11	26.70060102274432	25.14737830956933	25.857260845106644	22.294759822579707
12-13	26.89380223490544	22.082999692358136	26.678281319663466	24.344916753072958
14-15	23.877888256839267	23.603118992567595	24.38398798810889	28.13500476248425
16-17	24.280518082320654	26.691006324530246	24.861907448319485	24.16656814482962
18-19	24.216471638571026	25.966687717100466	26.45229984279065	23.36454080153786
20-21	24.80605309837714	25.031272169341328	26.475228360293347	23.687446371988187
22-23	24.44859238578843	24.73665120869903	26.441163139264866	24.373593266247674
24-25	24.287799126653532	25.0150809592542	26.48322777805219	24.213892136040087
26-27	24.306350259936448	25.130939971451234	26.469217702978966	24.093492065633352
28-29	24.250563289399732	25.315874220117994	26.38456923166274	24.048993258819532
30-31	24.427959417877897	25.15050270440798	26.242758907629494	24.17877897008463
32-33	24.234980894454335	25.206095165723706	26.24838729625524	24.310536643566724
34-35	24.44014237817365	25.202820540928695	26.332063403301987	24.02497367759567
36-37	24.463264539531306	25.16230607804455	26.219853319247033	24.154576063177114
38-39	24.430857583015406	25.22227200150717	26.206404273310905	24.140466142166513
40-41	24.554409716128937	25.174706374280404	26.14574765022256	24.125136259368098
42-43	24.447112029703316	25.16034182633601	26.101903915951404	24.290642228009272
44-45	24.41903200255242	25.181740582829615	26.082815839600737	24.316411575017224
46-47	24.560596602666557	25.260493479945943	26.020537655798766	24.15837226158873
48-49	24.577329839490897	25.35016245224304	25.754918573849334	24.31758913441673
50-51	24.380495694890165	25.57419848942512	25.738458504913797	24.30684731077092
52-53	24.56886062525368	25.546350290333336	25.615068780785155	24.269720303627825
54-55	24.55720073250552	25.305548783045705	25.76567938904074	24.371571095408033
56-57	24.51574770118608	25.514485562445007	25.70923578767996	24.260530948688952
58-59	24.68632542054545	25.397388190208662	25.657410163316978	24.25887622592891
60-61	24.669637765455416	25.354600667108418	25.741969797575653	24.233791769860513
62-63	24.45914966069605	25.48409200497416	25.77259353236208	24.284164801967716
64-65	24.520841511058418	25.351728861576078	25.728702460507446	24.398727166858055
66-67	24.502009342706074	25.456895922839777	25.65618365984628	24.384911074607867
68-69	24.498629303512345	25.440927384537265	25.6731166218536	24.38732669009679
70-71	24.508044731654994	25.305310452258308	25.71624340884594	24.470401407240754
72-73	24.646597738609696	25.326658986312108	25.582429428167263	24.44431384691093
74-75	24.48915552952324	25.5478440794965	25.48937429092659	24.473626100053668
76-77	24.538713808532826	25.341034400415364	25.649255922339094	24.47099586871272
78-79	24.491638844569632	25.31983353980915	25.741520031852673	24.44700758376854
80-81	24.50187493990211	25.476818535064638	25.61772629707687	24.40358022795638
82-83	24.604222729420595	25.366388449444425	25.583251767588106	24.446137053546877
84-85	24.584801924152636	25.469391749323307	25.52050448302459	24.425301843499465
86-87	24.51142728390623	25.472220397669158	25.611346700962667	24.405005617461942
88-89	24.46672324068476	25.370195603173933	25.554084140868845	24.608997015272458
90-91	24.535976897729054	25.367015904785532	25.553900110512213	24.5431070869732
92-93	24.634370583149988	25.505928013063585	25.621183498534005	24.238517905252422
94-95	24.603902370104464	25.34087498008167	25.555752928995172	24.4994697208187
96-97	24.536073848230348	25.311287317985894	25.652022780318617	24.500616053465144
98-99	24.52995164713969	25.386640554742733	25.54092754867036	24.542480249447216
100-101	25.088246281196547	24.72260718836832	25.72143621051265	24.46771031992249
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	288.0
1	238.0
2	401.5
3	588.0
4	416.0
5	320.5
6	546.0
7	1195.0
8	1817.5
9	1803.5
10	1371.0
11	1018.0
12	995.5
13	1155.5
14	1305.0
15	1432.0
16	1626.5
17	1874.0
18	2127.5
19	2312.0
20	2509.0
21	2890.0
22	3345.0
23	3812.0
24	4449.0
25	5470.5
26	6862.0
27	8584.0
28	10868.0
29	13960.0
30	18057.5
31	22576.0
32	28059.0
33	34489.5
34	41372.0
35	49252.5
36	58244.5
37	68029.5
38	77359.5
39	87750.5
40	99154.5
41	108622.0
42	118921.0
43	131773.0
44	144727.0
45	156921.5
46	167389.0
47	178472.5
48	191911.0
49	197663.0
50	194854.0
51	193522.0
52	194076.0
53	194073.5
54	192856.0
55	186732.5
56	174955.5
57	161578.5
58	148981.5
59	134248.0
60	115421.5
61	97138.0
62	79391.5
63	60570.5
64	44583.0
65	33282.0
66	25085.0
67	19250.0
68	14763.5
69	11072.0
70	8451.5
71	6439.5
72	4780.5
73	3699.5
74	2773.0
75	2068.0
76	1480.0
77	911.5
78	537.0
79	308.0
80	202.5
81	105.5
82	51.5
83	29.5
84	14.5
85	11.0
86	7.0
87	1.5
88	1.5
89	1.5
90	0.5
91	0.5
92	1.5
93	1.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3081727147933647
2	0.0
3	0.005920683416028593
4	0.059275413504873135
5	5.029151936394944E-4
6	0.06206430685141943
7	0.07321988023760456
8	0.10350909076361958
9	0.09109622939333568
10-11	0.09588535362367542
12-13	0.09494810258098363
14-15	0.06462460238267503
16-17	0.034792587487241386
18-19	0.0380272465736045
20-21	0.02737458838105884
22-23	0.04062183177715371
24-25	0.0327123473680962
26-27	0.0211681576959169
28-29	0.02461998470680616
30-31	0.0035432661370055287
32-33	0.005520637239269904
34-35	0.008446689274990598
36-37	0.01156704945370837
38-39	0.016996247566862004
40-41	0.021545344091146522
42-43	0.003851873187647946
44-45	0.013933036841966902
46-47	0.02434566732845734
48-49	0.0030289210526015004
50-51	0.0076008773584150865
52-53	0.0028917623634270927
54-55	0.004423367725874644
56-57	0.0039776019860578195
58-59	0.0013030075471568718
60-61	0.0055663568023280405
62-63	0.015727529691998733
64-65	0.007223690963185465
66-67	0.038221554716601575
68-69	0.06012122542144864
70-71	0.012470010824106554
72-73	0.03275806693115434
74-75	0.04918281995978964
76-77	0.29766864518075803
78-79	0.0713110884799274
80-81	0.032495179443570055
82-83	0.028197540516105287
84-85	0.018962188778361845
86-87	0.11621912929378135
88-89	0.5183226863900862
90-91	0.13137516444755337
92-93	0.04651965541165323
94-95	0.008778156107162084
96-97	0.006412168718903554
98-99	0.0115098999998857
100-101	0.008526698510342335
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4374495.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.412851523844274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.8396192682973	38.9057176903091
2	15.064139343133077	16.092372761385363
3	5.109498111926909	8.187385920411439
4	2.2657098787633916	4.84072101401985
5	1.2278224927222854	3.2790750250705902
6	0.7622320374720015	2.4427791986525595
7	0.5024828404970081	1.8787328946922355
8	0.36973299614576827	1.5798794901280007
9	0.2742257163443771	1.3182459724009845
>10	1.4502796895711465	13.982937691386995
>50	0.09577832490150662	3.482342674729767
>100	0.0371925155089989	3.2214442054751866
>500	9.865350869342924E-4	0.3429210852400458
>1k	2.5735682517308723E-4	0.30406510104257034
>5k	4.28928041955086E-5	0.14137927505527217
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6171	0.14106771181587818	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.2859781529067928E-5	0.0
5	0.0	0.0	0.0	4.5719563058135856E-5	0.0
6	4.5719563058135856E-5	0.0	0.0	4.5719563058135856E-5	0.0
7	4.5719563058135856E-5	0.0	0.0	4.5719563058135856E-5	0.0
8	6.857934458720378E-5	0.0	0.0	4.5719563058135856E-5	0.0
9	6.857934458720378E-5	0.0	0.0	4.5719563058135856E-5	0.0
10-11	6.857934458720378E-5	0.0	0.0	9.143912611627171E-5	2.2859781529067928E-5
12-13	6.857934458720378E-5	0.0	0.0	9.143912611627171E-5	1.0286901688080568E-4
14-15	6.857934458720378E-5	0.0	0.0	9.143912611627171E-5	1.1429890764533963E-4
16-17	1.1429890764533963E-4	0.0	0.0	1.2572879840987361E-4	1.3715868917440756E-4
18-19	1.1429890764533963E-4	2.2859781529067928E-5	0.0	1.600184707034755E-4	1.3715868917440756E-4
20-21	1.1429890764533963E-4	2.2859781529067928E-5	0.0	1.7144836146800946E-4	1.4858857993894152E-4
22-23	1.1429890764533963E-4	2.2859781529067928E-5	0.0	1.9430814299707737E-4	2.171679245261453E-4
24-25	1.1429890764533963E-4	2.2859781529067928E-5	0.0	2.743173783488151E-4	2.514575968197472E-4
26-27	1.1429890764533963E-4	2.2859781529067928E-5	0.0	2.9717715987788305E-4	2.9717715987788305E-4
28-29	1.1429890764533963E-4	2.2859781529067928E-5	0.0	3.8861628599415473E-4	2.9717715987788305E-4
30-31	1.1429890764533963E-4	2.2859781529067928E-5	0.0	0.0011772787487469981	2.9717715987788305E-4
32-33	1.1429890764533963E-4	2.2859781529067928E-5	0.0	0.0026174449850782776	2.9717715987788305E-4
34-35	1.1429890764533963E-4	2.2859781529067928E-5	0.0	0.004697685104223459	2.9717715987788305E-4
36-37	1.1429890764533963E-4	3.428967229360189E-5	0.0	0.007360849652359873	2.9717715987788305E-4
38-39	1.1429890764533963E-4	6.857934458720378E-5	0.0	0.012127114101170535	3.428967229360189E-4
40-41	1.257287984098736E-4	8.000923535173774E-5	0.0	0.017281994835975353	3.428967229360189E-4
42-43	1.3715868917440756E-4	1.1429890764533963E-4	0.0	0.022505454915367373	3.428967229360189E-4
44-45	1.3715868917440756E-4	1.1429890764533963E-4	0.0	0.028163250843811685	3.6575650446508685E-4
46-47	1.3715868917440756E-4	1.1429890764533963E-4	0.0	0.033706747864610656	4.0004617675868867E-4
48-49	1.3715868917440756E-4	1.1429890764533963E-4	0.0	0.039170235650057894	4.343358490522906E-4
50-51	1.3715868917440756E-4	1.1429890764533963E-4	0.0	0.04554811469666784	4.343358490522906E-4
52-53	1.3715868917440756E-4	1.1429890764533963E-4	0.0	0.05178883505410339	4.343358490522906E-4
54-55	1.3715868917440756E-4	1.1429890764533963E-4	0.0	0.05838388202523949	4.343358490522906E-4
56-57	1.3715868917440756E-4	1.1429890764533963E-4	0.0	0.0651846670301372	4.5719563058135854E-4
58-59	1.3715868917440756E-4	1.1429890764533963E-4	0.0	0.0724883672286744	4.8005541211042647E-4
60-61	1.3715868917440756E-4	1.257287984098736E-4	0.0	0.0798263570995052	5.029151936394944E-4
62-63	1.600184707034755E-4	1.3715868917440756E-4	0.0	0.0883759153913766	5.257749751685623E-4
64-65	1.600184707034755E-4	1.3715868917440756E-4	0.0	0.09708549215395149	5.486347566976302E-4
66-67	1.600184707034755E-4	1.3715868917440756E-4	0.0	0.10531501350441594	5.486347566976302E-4
68-69	1.600184707034755E-4	1.3715868917440756E-4	0.0	0.11387600168705186	5.486347566976302E-4
70-71	1.8287825223254342E-4	1.4858857993894152E-4	0.0	0.1231342132063244	5.943543197557661E-4
72-73	1.8287825223254342E-4	1.600184707034755E-4	0.0	0.13254101330553586	7.086532274011057E-4
74-75	1.8287825223254342E-4	1.600184707034755E-4	0.0	0.14149061777416594	7.543727904592416E-4
76-77	1.8287825223254342E-4	1.600184707034755E-4	0.0	0.15071453962114484	7.658026812237755E-4
78-79	1.8287825223254342E-4	1.7144836146800946E-4	0.0	0.1600184707034755	7.772325719883095E-4
80-81	1.8287825223254342E-4	1.8287825223254342E-4	0.0	0.1701567838116171	8.000923535173773E-4
82-83	1.8287825223254342E-4	1.9430814299707737E-4	0.0	0.1808894512395145	8.229521350464453E-4
84-85	1.8287825223254342E-4	2.0573803376161133E-4	0.0	0.19190786593652526	8.229521350464453E-4
86-87	1.8287825223254342E-4	2.514575968197472E-4	0.0	0.20320059801188478	8.572418073400473E-4
88-89	1.8287825223254342E-4	2.6288748758428117E-4	0.0	0.2154191512391716	8.686716981045812E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	5370	0.0	35.211956	1
GTATCAA	11395	0.0	33.940296	1
TACCTGG	5620	0.0	33.04642	2
TCAACGC	13895	0.0	27.416983	4
TATCAAC	14210	0.0	27.10883	2
CAACGCA	14255	0.0	26.64513	5
ATCAACG	14320	0.0	26.636454	3
GGTATCA	7495	0.0	25.991156	1
AACGCAG	15110	0.0	25.327684	6
ACCTGGG	7340	0.0	24.786007	3
GTACATG	13640	0.0	24.128904	1
TACATGG	13940	0.0	22.693184	2
ACGCAGA	17455	0.0	21.844667	7
ACATGGG	14180	0.0	20.903175	3
CGCAGAG	18285	0.0	20.827116	8
TATCACG	515	0.0	20.290813	2
AGAGTAC	14840	0.0	20.045164	10-11
GCAGAGT	19995	0.0	18.760117	9
GAGTACA	10710	0.0	18.633673	1
CATGGGG	11285	0.0	18.478504	4
>>END_MODULE