FastQCFastQC Report
Mon 15 Aug 2016
SRR2921428_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921428_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2739032
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57610.21032978074005704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37480.13683666346358858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35970.13132376693664038No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29760.10865152360395935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89050.024.7628171
GTACATG75450.023.4299031
TACATGG76850.022.2477572
TCAACGC104800.020.8630774
TATCAAC104850.020.8531282
CAACGCA105700.020.6854345
ACATGGG79100.020.4559423
ATCAACG106850.020.4199053
AACGCAG109350.020.0310346
GGTATCA66650.020.030711
GAGTACT49400.018.68749812-13
GTACCTG25650.018.4862291
GAGTACA60200.018.4297331
AGAGTAC87600.018.14152310-11
ACGCAGA121450.017.95537
TACCTGG26300.017.9515822
GTACTTT52200.017.69172914-15
AGTACAT57650.017.4126992
GTATAAG7450.017.3021561
AGTACTT54750.017.2379712-13
Produced by FastQC (version 0.11.5)