##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921428_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2739032
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.951041462823362	31.0	31.0	34.0	28.0	34.0
2	31.07026862044693	33.0	31.0	34.0	28.0	34.0
3	31.201217437401244	33.0	31.0	34.0	28.0	34.0
4	34.72305617459015	37.0	35.0	37.0	32.0	37.0
5	34.733984853042976	37.0	35.0	37.0	32.0	37.0
6	34.83525384150313	37.0	35.0	37.0	32.0	37.0
7	34.744974867033314	37.0	35.0	37.0	32.0	37.0
8	34.73936157007293	37.0	35.0	37.0	32.0	37.0
9	36.27609535047418	39.0	37.0	39.0	32.0	39.0
10-11	36.179504657119736	39.0	37.0	39.0	32.0	39.0
12-13	36.05027323521594	39.0	37.0	39.0	32.0	39.0
14-15	37.178189959080434	40.0	38.0	41.0	32.0	41.0
16-17	37.069794000216135	40.0	37.5	41.0	31.5	41.0
18-19	36.92339921548927	40.0	37.0	41.0	31.0	41.0
20-21	36.8268808104469	40.0	37.0	41.0	31.0	41.0
22-23	36.86936132911189	40.0	37.0	41.0	31.0	41.0
24-25	36.76706277984339	40.0	37.0	41.0	31.0	41.0
26-27	36.54056834677361	40.0	37.0	41.0	30.0	41.0
28-29	36.347021685033255	40.0	36.5	41.0	30.0	41.0
30-31	36.12780975176632	39.0	36.0	41.0	29.5	41.0
32-33	35.88275146110013	39.0	36.0	40.0	28.5	41.0
34-35	35.49262257615099	39.0	35.0	40.0	27.0	41.0
36-37	35.142725422704075	38.5	35.0	40.0	25.5	41.0
38-39	34.94270183772953	38.0	35.0	40.0	25.0	41.0
40-41	34.71567163143767	38.0	34.5	40.0	24.0	41.0
42-43	34.45080579562415	38.0	34.0	40.0	24.0	41.0
44-45	34.159628839677666	38.0	33.5	40.0	23.0	41.0
46-47	33.88786293843957	38.0	33.0	40.0	21.5	41.0
48-49	33.70060353438733	38.0	33.0	40.0	20.0	41.0
50-51	32.89005440608214	37.0	32.0	39.0	19.5	40.5
52-53	33.33520199837022	37.5	33.0	39.0	21.5	40.0
54-55	33.78981260532918	38.0	34.0	40.0	21.0	41.0
56-57	33.660067133206184	38.0	33.5	40.0	20.0	41.0
58-59	33.356720366903346	37.0	33.0	40.0	18.5	41.0
60-61	32.933806724419426	36.5	33.0	39.5	17.0	41.0
62-63	32.47938267972043	36.0	32.0	39.0	12.5	40.5
64-65	31.97345449049153	35.5	31.5	39.0	8.0	40.0
66-67	31.51791946935998	35.0	31.0	38.0	7.0	40.0
68-69	31.026727508112355	35.0	31.0	37.5	2.0	40.0
70-71	30.536471643996858	35.0	30.0	37.0	2.0	39.0
72-73	30.01505988246943	34.0	29.5	36.0	2.0	39.0
74-75	29.459387111943197	34.0	29.0	36.0	2.0	38.5
76-77	28.92039870289942	34.0	29.0	35.0	2.0	37.0
78-79	28.400822078748988	34.0	28.0	35.0	2.0	37.0
80-81	27.868173318164956	33.0	27.0	35.0	2.0	36.0
82-83	27.293784081383496	33.0	26.0	35.0	2.0	36.0
84-85	26.732726926885118	32.0	25.0	34.5	2.0	35.0
86-87	26.171673970950323	32.0	24.5	34.0	2.0	35.0
88-89	25.577065547244427	32.0	22.5	34.0	2.0	35.0
90-91	24.889077418591675	31.0	19.5	34.0	2.0	35.0
92-93	24.128377288034606	31.0	14.5	34.0	2.0	35.0
94-95	23.27645898258947	30.5	2.0	34.0	2.0	35.0
96-97	22.40964636411696	30.0	2.0	34.0	2.0	35.0
98	21.798723052523666	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59624.0
3	10422.0
4	8169.0
5	7018.0
6	6524.0
7	6776.0
8	7667.0
9	8822.0
10	9993.0
11	11273.0
12	12928.0
13	16603.0
14	13248.0
15	12532.0
16	14271.0
17	16902.0
18	19266.0
19	17967.0
20	17995.0
21	19474.0
22	21338.0
23	23812.0
24	26879.0
25	30769.0
26	35341.0
27	41149.0
28	48459.0
29	58172.0
30	70805.0
31	87271.0
32	112280.0
33	149324.0
34	210774.0
35	314410.0
36	452192.0
37	511423.0
38	236444.0
39	10716.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.34934083104534	17.517535149194636	13.531329882448265	24.601794137311757
2	16.25890008778169	19.16037162740966	38.17911628717434	26.401611997634316
3	19.89645872925759	22.18257195318248	28.432092460140883	29.488876857419044
4	14.0930709512295	15.082757620470453	34.58883854382243	36.235332884477614
5	15.415063663433259	35.716738824646974	33.02745656820299	15.840740943716774
6	34.93091426704966	33.37463107875167	16.388579754581052	15.305874899617617
7	30.568308793420922	30.72249218216068	19.605581285256072	19.103617739162328
8	27.629950020486998	33.4018789609111	19.203110518047183	19.765060500554718
9	26.75586065873074	16.153436062221573	19.378003359380706	37.71269991966698
10-11	26.472118698470915	24.94033234228056	26.3025004181118	22.285048541136725
12-13	26.609761365435112	22.17210819882179	26.71291462360837	24.505215812134733
14-15	23.80731456645248	23.872021652773086	24.434191852494138	27.886471928280304
16-17	24.058608612364367	26.79935783288866	25.14939076102426	23.992642793722712
18-19	24.06942850765532	26.1750756289689	26.544772605307926	23.21072325806785
20-21	24.609835492874574	25.271912906014826	26.490167503612227	23.628084097498373
22-23	24.474885894815635	24.9470225929727	26.320085874933113	24.25800563727855
24-25	24.332368987575798	25.127954237543275	26.376074384453158	24.163602390427766
26-27	24.208720310909797	25.28369422817958	26.50739909043901	24.000186370471607
28-29	24.16234244272318	25.530952697232213	26.358017055461584	23.94868780458302
30-31	24.40098193424387	25.350456598692737	26.126253333408833	24.122308133654556
32-33	24.268054366764737	25.430489384521575	26.041710989516098	24.259745259197587
34-35	24.475048835331204	25.30384238494249	26.20342081068025	24.017687969046055
36-37	24.48273314499571	25.217613757908968	26.191922800687777	24.107730296407546
38-39	24.44593427988906	25.36783424866374	26.14367319949305	24.042558271954153
40-41	24.548723996563947	25.392438072319873	26.036744182373155	24.022093748743025
42-43	24.507010598939317	25.277083041458393	25.886912697886622	24.32899366171567
44-45	24.463781930243687	25.314545223225082	25.85814810415512	24.36352474237611
46-47	24.59388254782598	25.349670182734958	25.888021100081097	24.16842616935796
48-49	24.509158081319143	25.49703669454836	25.723480990481335	24.27032423365116
50-51	24.35169632317981	25.66660608697408	25.768361613035278	24.21333597681083
52-53	24.563572168347463	25.622445348110624	25.586985443249727	24.22699704029219
54-55	24.48663072530721	25.379654486865828	25.71427206991961	24.41944271790735
56-57	24.520473904483264	25.464710154665937	25.7147819394599	24.300034001390898
58-59	24.60611754029682	25.435677061536634	25.668052845116584	24.29015255304996
60-61	24.567571363139454	25.400752480809352	25.855655492660144	24.176020663391046
62-63	24.39865628161905	25.4974179669549	25.766132267435722	24.337793483990332
64-65	24.50141864197098	25.43872680486865	25.672782989738145	24.38707156342223
66-67	24.477421021009327	25.469632733986124	25.620894599593218	24.432051645411327
68-69	24.41958027064957	25.46244107493132	25.66514918626674	24.452829468152373
70-71	24.512689487216562	25.331559048668296	25.717084291488646	24.438667172626495
72-73	24.589552190886554	25.357711395596855	25.62217318054787	24.430563232968723
74-75	24.38991373692155	25.634711107923586	25.55924994465142	24.416125210503445
76-77	24.557254249874884	25.4248590713163	25.50755973600363	24.51032694280519
78-79	24.497519585670005	25.37461199671932	25.603382821771	24.524485595839675
80-81	24.515322856392338	25.456574600877858	25.54502790443081	24.483074638298994
82-83	24.63440996539823	25.38818512525643	25.540415305655706	24.436989603689632
84-85	24.61730784785081	25.45366143351896	25.481630978444375	24.447399740185855
86-87	24.548172910421794	25.43474205821163	25.600860285895028	24.41622474547155
88-89	24.51209061249037	25.260418625566476	25.564534668146393	24.662956093796755
90-91	24.561112267761644	25.251317740868906	25.549727463581558	24.637842527787893
92-93	24.64183023703366	25.438013721495174	25.525102881866157	24.39505315960501
94-95	24.7005026583972	25.266247885563903	25.545781795147764	24.487467660891138
96-97	24.60946843683184	25.232392878054366	25.583559446644838	24.574579238468957
98	24.646642882691197	25.351908067903555	25.39772737694544	24.60372167245981
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	992.0
1	757.5
2	846.5
3	1142.0
4	924.0
5	843.5
6	1345.0
7	2763.0
8	4046.0
9	3867.5
10	2786.5
11	1990.5
12	1892.5
13	2009.5
14	2224.5
15	2493.5
16	2679.5
17	2705.0
18	2655.0
19	2666.5
20	2615.5
21	2464.5
22	2407.0
23	2472.0
24	2630.0
25	3020.0
26	3684.5
27	4695.5
28	6129.5
29	8303.5
30	11210.5
31	14767.5
32	19111.0
33	23426.5
34	28238.5
35	33914.0
36	39889.5
37	46303.0
38	53393.0
39	60342.5
40	67261.5
41	75020.0
42	82814.0
43	90634.0
44	97925.0
45	105331.0
46	114865.5
47	124066.0
48	127288.5
49	125072.0
50	123423.0
51	122824.0
52	122125.0
53	122394.0
54	121204.5
55	116338.5
56	108333.0
57	100432.0
58	92168.5
59	82136.0
60	71782.0
61	60595.5
62	49137.0
63	37475.5
64	27443.5
65	20749.5
66	16032.5
67	12274.0
68	9186.0
69	6919.0
70	5176.5
71	3932.0
72	3041.0
73	2151.5
74	1581.5
75	1203.5
76	797.0
77	475.0
78	276.0
79	150.0
80	83.5
81	47.0
82	34.5
83	22.0
84	12.0
85	11.5
86	8.0
87	7.5
88	7.0
89	6.0
90	6.0
91	5.0
92	5.0
93	3.5
94	2.0
95	2.5
96	2.5
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5087198689171941
2	0.056917918447101014
3	0.06447533289132802
4	0.0621022317373437
5	0.07236133057226056
6	0.07294547854862594
7	0.050492290707081916
8	0.02592156645121342
9	0.015771995361865066
10-11	0.019349901717102977
12-13	0.014183843051121709
14-15	0.019623721081024247
16-17	0.017980804897496632
18-19	0.06042280630529326
20-21	0.0910540658159525
22-23	0.10806737562759397
24-25	0.07725356987432057
26-27	0.0929890559876628
28-29	0.09516135627477151
30-31	0.06504122624343199
32-33	0.0391561690407414
34-35	0.42914796176167347
36-37	0.6712590433408592
38-39	0.6163491335625141
40-41	0.6105624176716445
42-43	0.5832900090250862
44-45	0.5672259396750385
46-47	0.5568025492217689
48-49	0.5462696310229307
50-51	0.5603074370799611
52-53	0.6443334725552677
54-55	0.6140855601540982
56-57	0.5700554064355583
58-59	0.5474926908484458
60-61	0.5613114414143391
62-63	0.5729578916931236
64-65	0.5907744049722676
66-67	0.6181563413643943
68-69	0.5716618133705631
70-71	0.5176281255567661
72-73	0.49964732065926937
74-75	0.4792386507350042
76-77	0.4943352249991968
78-79	0.4884389813627588
80-81	0.48557300535371617
82-83	0.5068396426182681
84-85	0.4692351166397472
86-87	0.47321462472873627
88-89	0.5022394773043908
90-91	0.483729288303313
92-93	0.4779060631639207
94-95	0.49008189754628645
96-97	0.48411263541280275
98	0.4785267203888089
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2739032.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.39098638997747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95659040475803	44.51745840568101
2	14.557996561330224	17.292275512786045
3	4.683756556276304	8.345187656633213
4	2.040072922249771	4.846477726395909
5	1.0526058509005471	3.1257649882422522
6	0.6188980908527145	2.2054180854369965
7	0.4178600833889357	1.7371985767826992
8	0.29921144337737754	1.4216370209081075
9	0.22308355141006292	1.1924236949060598
>10	1.0915881593532752	11.339826219518532
>50	0.04555867379584507	1.8036617372482415
>100	0.01148161411866643	1.098452582685185
>500	9.25777277310892E-4	0.39686603572433016
>1k	3.0859242573793086E-4	0.4661810512571642
>5k	6.171848514758617E-5	0.21117070579434838
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5761	0.21032978074005704	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3748	0.13683666346358858	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3597	0.13132376693664038	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	2976	0.10865152360395935	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.650924852283581E-5
2	0.0	0.0	0.0	0.0	3.650924852283581E-5
3	0.0	0.0	0.0	0.0	3.650924852283581E-5
4	0.0	0.0	0.0	0.0	3.650924852283581E-5
5	0.0	0.0	0.0	0.0	3.650924852283581E-5
6	0.0	0.0	0.0	0.0	7.301849704567161E-5
7	0.0	0.0	0.0	0.0	7.301849704567161E-5
8	0.0	0.0	0.0	0.0	7.301849704567161E-5
9	0.0	0.0	0.0	0.0	1.0952774556850741E-4
10-11	3.650924852283581E-5	0.0	0.0	0.0	1.0952774556850741E-4
12-13	3.650924852283581E-5	0.0	0.0	1.6429161835276114E-4	2.5556473965985063E-4
14-15	5.4763872784253714E-5	3.650924852283581E-5	0.0	1.8254624261417903E-4	3.1032861244410434E-4
16-17	1.2778236982992531E-4	7.301849704567161E-5	0.0	2.0080086687559692E-4	3.2858323670552223E-4
18-19	1.4603699409134323E-4	7.301849704567161E-5	0.0	2.1905549113701483E-4	3.2858323670552223E-4
20-21	1.4603699409134323E-4	7.301849704567161E-5	0.0	3.1032861244410434E-4	3.468378609669401E-4
22-23	1.4603699409134323E-4	7.301849704567161E-5	0.0	3.2858323670552223E-4	4.0160173375119383E-4
24-25	1.6429161835276114E-4	7.301849704567161E-5	0.0	4.5636560653544754E-4	4.0160173375119383E-4
26-27	1.8254624261417903E-4	7.301849704567161E-5	0.0	6.571664734110446E-4	4.1985635801261177E-4
28-29	1.8254624261417903E-4	7.301849704567161E-5	0.0	0.0020080086687559694	4.746202307968655E-4
30-31	2.5556473965985063E-4	9.127312130708951E-5	0.0	0.007831233808148281	4.746202307968655E-4
32-33	2.9207398818268646E-4	1.0952774556850741E-4	0.0	0.020043577439036855	5.111294793197013E-4
34-35	2.9207398818268646E-4	1.0952774556850741E-4	0.0	0.03607113754056178	6.206572248882087E-4
36-37	2.9207398818268646E-4	1.0952774556850741E-4	0.0	0.05901720023716408	6.206572248882087E-4
38-39	2.9207398818268646E-4	1.4603699409134323E-4	0.0	0.0919667970290234	6.206572248882087E-4
40-41	2.9207398818268646E-4	1.8254624261417903E-4	0.0	0.1267601108712859	6.936757219338803E-4
42-43	2.9207398818268646E-4	1.8254624261417903E-4	0.0	0.16175422558042402	6.936757219338803E-4
44-45	2.9207398818268646E-4	1.8254624261417903E-4	0.0	0.20038101051758433	7.301849704567161E-4
46-47	2.9207398818268646E-4	1.8254624261417903E-4	0.0	0.2407419847595793	8.032034675023877E-4
48-49	2.9207398818268646E-4	1.8254624261417903E-4	0.0	0.28159583385663256	8.397127160252235E-4
50-51	2.9207398818268646E-4	1.8254624261417903E-4	0.0	0.3186527211073109	8.397127160252235E-4
52-53	2.9207398818268646E-4	1.8254624261417903E-4	0.0	0.3558921546006034	8.944765888094773E-4
54-55	2.9207398818268646E-4	1.8254624261417903E-4	0.0	0.39490228664725346	9.49240461593731E-4
56-57	2.9207398818268646E-4	1.8254624261417903E-4	0.0	0.43765461666749417	9.49240461593731E-4
58-59	2.9207398818268646E-4	1.8254624261417903E-4	0.0	0.47876403050420735	9.674950858551488E-4
60-61	2.9207398818268646E-4	1.8254624261417903E-4	0.0	0.5183218012786999	0.0010040043343779847
62-63	2.9207398818268646E-4	1.8254624261417903E-4	0.0	0.559339941994106	0.0011682959527307458
64-65	2.9207398818268646E-4	1.8254624261417903E-4	0.0	0.60380820669492	0.0011682959527307458
66-67	2.9207398818268646E-4	2.1905549113701483E-4	0.0	0.6546290806387074	0.0011865505769921637
68-69	2.9207398818268646E-4	2.1905549113701483E-4	0.0	0.7036427467806144	0.0012048052012535815
70-71	2.9207398818268646E-4	2.1905549113701483E-4	0.0	0.7508126958721184	0.001296078322560671
72-73	4.0160173375119383E-4	2.1905549113701483E-4	0.0	0.7974167516115183	0.0013690968196063426
74-75	4.0160173375119383E-4	2.1905549113701483E-4	0.0	0.8467042371173465	0.0013873514438677607
76-77	4.0160173375119383E-4	2.1905549113701483E-4	0.0	0.8988576986322174	0.0014238606923905963
78-79	4.0160173375119383E-4	2.1905549113701483E-4	0.0	0.9549906682360776	0.0014603699409134322
80-81	4.0160173375119383E-4	2.1905549113701483E-4	0.0	1.0125474985323282	0.0015516430622205216
82-83	4.0160173375119383E-4	2.1905549113701483E-4	0.0	1.0682971210266985	0.001679425432050447
84-85	4.1985635801261177E-4	2.1905549113701483E-4	0.0	1.125415840340675	0.001679425432050447
86	4.3811098227402966E-4	2.1905549113701483E-4	0.0	1.1704865076421158	0.001679425432050447
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8905	0.0	24.762817	1
GTACATG	7545	0.0	23.429903	1
TACATGG	7685	0.0	22.247757	2
TCAACGC	10480	0.0	20.863077	4
TATCAAC	10485	0.0	20.853128	2
CAACGCA	10570	0.0	20.685434	5
ACATGGG	7910	0.0	20.455942	3
ATCAACG	10685	0.0	20.419905	3
AACGCAG	10935	0.0	20.031034	6
GGTATCA	6665	0.0	20.03071	1
GAGTACT	4940	0.0	18.687498	12-13
GTACCTG	2565	0.0	18.486229	1
GAGTACA	6020	0.0	18.429733	1
AGAGTAC	8760	0.0	18.141523	10-11
ACGCAGA	12145	0.0	17.9553	7
TACCTGG	2630	0.0	17.951582	2
GTACTTT	5220	0.0	17.691729	14-15
AGTACAT	5765	0.0	17.412699	2
GTATAAG	745	0.0	17.302156	1
AGTACTT	5475	0.0	17.23797	12-13
>>END_MODULE