FastQCFastQC Report
Mon 15 Aug 2016
SRR2921428_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921428_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2739032
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55660.2032104772781041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38870.14191144900826277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36070.13168885942186875No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30870.11270405018999413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95200.024.7223321
GTACATG75250.023.9323181
TATACCG2801.8189894E-1223.7426765
TACATGG78950.022.203662
TCAACGC108800.021.1777134
CAACGCA109350.020.9309025
TATCAAC111450.020.9291572
ACATGGG81350.020.67343
ATCAACG113050.020.4656053
AACGCAG114300.020.1082696
CATGGGG65100.018.9010094
AGAGTAC89500.018.67631510-11
GAGTACT49450.018.14628412-13
ACGCAGA126900.018.1130287
GAGTACA63600.017.8286841
GTACCTG26800.017.7772541
GGTATCA71500.017.7245431
CGCAGAG133050.017.2400918
GTACTTT51000.017.1214814-15
AGTACAT59100.016.8803772
Produced by FastQC (version 0.11.5)