##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921428_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2739032
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04335509771335	33.0	31.0	34.0	30.0	34.0
2	32.15812338081483	34.0	31.0	34.0	30.0	34.0
3	32.27927129000318	34.0	31.0	34.0	30.0	34.0
4	35.788862999775105	37.0	35.0	37.0	35.0	37.0
5	35.60235221786383	37.0	35.0	37.0	35.0	37.0
6	35.639655177449555	37.0	35.0	37.0	35.0	37.0
7	35.57389800484259	37.0	35.0	37.0	35.0	37.0
8	35.53328949789561	37.0	35.0	37.0	33.0	37.0
9	37.18596314318343	39.0	38.0	39.0	34.0	39.0
10-11	37.100616933281536	39.0	37.0	39.0	34.0	39.0
12-13	36.99307802902631	39.0	37.0	39.0	34.0	39.0
14-15	38.32841073050625	40.0	38.0	41.0	34.0	41.0
16-17	38.242401877743674	40.0	38.0	41.0	33.5	41.0
18-19	38.161544114855175	40.0	38.0	41.0	33.5	41.0
20-21	38.06432199404753	40.0	38.0	41.0	33.0	41.0
22-23	38.029439232546395	40.0	38.0	41.0	33.5	41.0
24-25	38.07491314449776	40.0	38.0	41.0	33.5	41.0
26-27	37.97466805791243	40.0	38.0	41.0	33.0	41.0
28-29	37.85482261616512	40.0	38.0	41.0	33.0	41.0
30-31	37.72484348485158	40.0	38.0	41.0	33.0	41.0
32-33	37.571459552133746	40.0	38.0	41.0	33.0	41.0
34-35	37.428990789446786	40.0	37.5	41.0	32.0	41.0
36-37	37.334763157202985	40.0	37.0	41.0	32.0	41.0
38-39	37.316561471351925	40.0	37.0	41.0	32.0	41.0
40-41	37.1694129531893	40.0	37.0	41.0	31.5	41.0
42-43	36.955696026917536	40.0	37.0	41.0	31.0	41.0
44-45	36.76003182876286	40.0	36.0	41.0	31.0	41.0
46-47	36.49869808019768	39.5	36.0	41.0	30.0	41.0
48-49	36.1844020077166	39.0	35.0	40.5	30.0	41.0
50-51	35.93293853448956	39.0	35.0	40.0	29.0	41.0
52-53	35.656138738065124	39.0	35.0	40.0	28.5	41.0
54-55	35.356010627112056	38.0	35.0	40.0	28.0	41.0
56-57	35.002133965576164	38.0	34.0	40.0	27.5	41.0
58-59	34.64262812555677	37.5	34.0	40.0	26.5	41.0
60-61	34.24760572348187	37.0	34.0	40.0	26.0	41.0
62-63	33.843843554949345	36.5	33.0	39.0	26.0	40.5
64-65	33.36248298669019	36.0	33.0	39.0	25.0	40.0
66-67	32.866426715715626	35.0	32.0	38.5	23.0	40.0
68-69	32.392160624629426	35.0	32.0	38.0	22.0	40.0
70-71	31.871170362376198	35.0	31.0	37.0	20.5	39.0
72-73	31.36179478735553	34.5	31.0	36.5	20.0	39.0
74-75	30.87459073132406	34.0	30.0	36.0	18.0	38.5
76-77	29.489746377552365	32.5	28.5	34.5	18.0	36.0
78-79	30.432655587813507	34.0	30.5	35.0	18.0	37.0
80-81	30.48599578245161	34.0	31.0	35.0	18.0	37.0
82-83	30.192182311123055	34.0	30.5	35.0	16.0	36.0
84-85	29.75791502253351	34.0	30.0	35.0	9.0	36.0
86-87	29.302665321179163	34.0	29.5	35.0	6.0	35.0
88-89	28.805714756162033	34.0	29.0	35.0	2.0	35.0
90-91	28.4021762067767	33.0	29.0	35.0	2.0	35.0
92-93	28.042691177028964	33.0	27.5	35.0	2.0	35.0
94-95	27.62893606208325	33.0	27.0	35.0	2.0	35.0
96-97	26.971494491484584	33.0	26.0	34.0	2.0	35.0
98-99	26.08059708685404	32.5	24.5	34.0	2.0	35.0
100-101	22.47082326895049	28.5	10.5	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17518.0
3	9499.0
4	5251.0
5	3903.0
6	3440.0
7	3564.0
8	4049.0
9	4758.0
10	5552.0
11	6426.0
12	7760.0
13	9127.0
14	10145.0
15	11304.0
16	12971.0
17	15387.0
18	15734.0
19	13017.0
20	12810.0
21	13226.0
22	14467.0
23	15859.0
24	18173.0
25	21404.0
26	25738.0
27	31177.0
28	38133.0
29	46735.0
30	58302.0
31	73088.0
32	95100.0
33	130573.0
34	191297.0
35	303529.0
36	498109.0
37	664651.0
38	316663.0
39	10593.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.79672497936766	17.845100454699136	13.766618057486307	24.5915565084469
2	16.304190677582444	19.304812795177273	37.90956074992917	26.48143577731111
3	19.82574489075374	22.274064591884645	28.730916316216465	29.16927420114515
4	14.231582239219179	15.304129515253056	34.59613432889258	35.86815391663519
5	15.546889004826925	35.66959022800565	33.03495312724191	15.748567639925506
6	34.9770764960742	33.41018951484885	16.355679837436572	15.257054151640375
7	30.70078258537444	30.704728762442336	19.603840361060588	18.990648291122636
8	27.69927243567017	33.375999368415975	19.175994836215786	19.74873335969807
9	26.685811475044385	16.17146712138632	19.38473975071793	37.75798165285136
10-11	26.435849873392414	24.92041456605769	26.348594159696415	22.29514140085348
12-13	26.62661493820927	22.10843350376972	26.826654299201653	24.438297258819354
14-15	23.813900980766455	23.801808088354836	24.51254865927819	27.871742271600517
16-17	23.99978013132223	26.81227305498397	25.27702722938439	23.91091958430941
18-19	23.998732613382252	26.146825096081237	26.70089584510855	23.15354644542796
20-21	24.585880911043738	25.24545124537017	26.560684268843065	23.607983574743027
22-23	24.44421027563576	24.831051465839927	26.444746469448642	24.279991789075673
24-25	24.28227751570834	25.119611283416287	26.491671402421296	24.106439798454073
26-27	24.169609036388977	25.266821718504595	26.63104783418583	23.9325214109206
28-29	24.170825964976988	25.516658343505238	26.458395909204196	23.85411978231357
30-31	24.381916879156698	25.271198619033846	26.24700980521017	24.099874696599283
32-33	24.240162211669286	25.2810581511017	26.212731031050712	24.266048606178305
34-35	24.465492542098172	25.216020189816255	26.29314995418625	24.02533731389932
36-37	24.333131415147076	25.219380640610094	26.326315854813153	24.121172089429674
38-39	24.32903036397775	25.348794664668546	26.279826654309872	24.042348317043835
40-41	24.487297488419106	25.251925858180226	26.175786378776788	24.08499027462388
42-43	24.410285543833428	25.235753431584545	26.014597298252983	24.339363726329044
44-45	24.37722819511335	25.217504341140724	26.01914794056211	24.38611952318382
46-47	24.51909268372311	25.311014211349615	25.987628634068198	24.182264470859074
48-49	24.499285586891236	25.36264200865912	25.9180167373656	24.22005566708404
50-51	24.293896084170186	25.589028461767644	25.883332749142703	24.23374270491947
52-53	24.530403623692006	25.55695571462969	25.673240112312108	24.239400549366195
54-55	24.47117356723101	25.32286009970724	25.762504433775597	24.44346189928616
56-57	24.45602031446529	25.45268507649871	25.762440510636438	24.32885409839956
58-59	24.59777681270378	25.373572172718063	25.705975055453724	24.32267595912443
60-61	24.56461265413674	25.33560214145694	25.89829503487371	24.201490169532615
62-63	24.370820180347295	25.44540012159475	25.807865683441022	24.37591401461693
64-65	24.427342672470843	25.404656911293404	25.72597995678816	24.442020459447594
66-67	24.489189546038183	25.371224997292664	25.686536372297525	24.453049084371635
68-69	24.365994528469077	25.397190145837474	25.72459790531369	24.512217420379763
70-71	24.43102359157115	25.31159057797706	25.751618474526143	24.50576735592564
72-73	24.565088970798367	25.245994739086253	25.672820834021913	24.516095456093463
74-75	24.27230304192579	25.582093641270102	25.607535866597264	24.538067450206842
76-77	24.4775017292838	25.2962583188303	25.621402132792515	24.60483781909338
78-79	24.366474690684218	25.335440349667277	25.742344177464364	24.55574078218414
80-81	24.35077646783831	25.421651984631342	25.702418518356197	24.525153029174156
82-83	24.470094871643173	25.318107416991875	25.671365329231406	24.54043238213354
84-85	24.411334884556567	25.43533210345578	25.588519049225777	24.56481396276188
86-87	24.378835614460456	25.419260601182366	25.669305470720754	24.532598313636427
88-89	24.27706694683856	25.274224846753462	25.6335805395071	24.815127666900878
90-91	24.362513332669174	25.244283746619423	25.63237752452434	24.760825396187062
92-93	24.437254872594934	25.376323190289913	25.63793853537049	24.54848340174466
94-95	24.401017310749875	25.262474534826424	25.62900507687794	24.707503077545756
96-97	24.264288550447493	25.298896896065425	25.726063283571193	24.71075126991589
98-99	24.322371366123573	25.298403427559073	25.632863555603265	24.74636165071409
100-101	24.654945628708845	24.801250179138183	25.751006596849173	24.792797595303796
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	613.0
1	515.5
2	693.5
3	956.5
4	762.0
5	702.5
6	1216.5
7	2641.0
8	3748.5
9	3482.0
10	2387.0
11	1470.0
12	1252.0
13	1235.0
14	1269.5
15	1259.5
16	1259.0
17	1352.0
18	1503.5
19	1558.0
20	1603.0
21	1655.0
22	1735.5
23	1837.0
24	1998.0
25	2392.0
26	2978.5
27	3811.5
28	5038.0
29	6901.0
30	9502.5
31	12371.5
32	16024.5
33	20261.5
34	24703.0
35	29714.5
36	35118.5
37	41208.5
38	47521.0
39	53989.5
40	61525.5
41	68822.5
42	75469.5
43	82851.0
44	90318.5
45	97898.5
46	105394.0
47	113616.5
48	123998.0
49	127600.5
50	123164.5
51	120923.5
52	121062.0
53	121101.0
54	120186.0
55	116418.5
56	108737.0
57	99798.0
58	91961.0
59	83038.5
60	72000.5
61	61044.0
62	49954.5
63	38068.5
64	28123.5
65	21166.5
66	16186.5
67	12494.0
68	9511.0
69	7069.0
70	5361.0
71	4109.5
72	3024.0
73	2247.0
74	1647.5
75	1188.0
76	859.5
77	532.5
78	297.5
79	146.5
80	74.0
81	49.0
82	28.0
83	12.0
84	9.5
85	5.0
86	1.5
87	1.5
88	0.5
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3320516153151916
2	0.0
3	0.006352609242973431
4	0.06608173982633281
5	7.666942189795519E-4
6	0.06881993346554549
7	0.08053940224137579
8	0.11149924498874056
9	0.09835591552051966
10-11	0.10301084470718122
12-13	0.10297433545865839
14-15	0.06889295196259117
16-17	0.03775056297261222
18-19	0.040689557478700504
20-21	0.029572491303497005
22-23	0.04439524620376834
24-25	0.03636321152874446
26-27	0.021649984374041635
28-29	0.02517312685649529
30-31	0.002920739881826864
32-33	0.005166058665981266
34-35	0.008232835541899474
36-37	0.010916265308327906
38-39	0.0167212358234588
40-41	0.021084091021937677
42-43	0.003669179476544998
44-45	0.014914028021578426
46-47	0.025282654602063793
48-49	0.002573902020859924
50-51	0.007301849704567162
52-53	0.0020992817900630585
54-55	0.004143799707341864
56-57	0.003541397106715073
58-59	0.001332587571083507
60-61	0.005439878029902535
62-63	0.015589449119250887
64-65	0.0072835950803057424
66-67	0.04028795574494931
68-69	0.06471264300672647
70-71	0.012486162994809844
72-73	0.03338770777413334
74-75	0.05319397509777177
76-77	0.32330765029397246
78-79	0.07829408345722139
80-81	0.03621717453465312
82-83	0.02942645430940566
84-85	0.02000706819051402
86-87	0.12132023284138337
88-89	0.5472736353573088
90-91	0.1365263348511445
92-93	0.05202567914504102
94-95	0.008853492766787683
96-97	0.006188317624620669
98-99	0.010587682071622384
100-101	0.008196326293376638
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2739032.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.89217589943999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.74175959169806	41.38436981925661
2	15.570130234894986	17.71637176201669
3	5.193468251639466	8.864031279012886
4	2.2704951561994893	5.166936392213112
5	1.1657755345710512	3.3161753386039967
6	0.7116346289376758	2.429186549139297
7	0.47714878082220896	1.9002222658118313
8	0.3381485663416294	1.5390406173161144
9	0.2461989174325008	1.2606112905139382
>10	1.2164550086425827	12.117316258250927
>50	0.05412958878860551	2.051120724679391
>100	0.013371126182079859	1.2061112525545767
>500	8.992296947850047E-4	0.36889389814972956
>1k	3.211534623910364E-4	0.4762184527331669
>5k	6.423069247820728E-5	0.2033940997477599
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5566	0.2032104772781041	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3887	0.14191144900826277	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3607	0.13168885942186875	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	3087	0.11270405018999413	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0952774556850741E-4
2	0.0	0.0	0.0	0.0	1.0952774556850741E-4
3	0.0	0.0	0.0	3.650924852283581E-5	1.0952774556850741E-4
4	0.0	0.0	0.0	3.650924852283581E-5	1.0952774556850741E-4
5	0.0	0.0	0.0	3.650924852283581E-5	1.4603699409134323E-4
6	0.0	0.0	0.0	3.650924852283581E-5	1.8254624261417903E-4
7	0.0	0.0	0.0	3.650924852283581E-5	1.8254624261417903E-4
8	0.0	0.0	0.0	3.650924852283581E-5	1.8254624261417903E-4
9	0.0	0.0	0.0	1.4603699409134323E-4	1.8254624261417903E-4
10-11	0.0	0.0	0.0	1.4603699409134323E-4	2.0080086687559692E-4
12-13	0.0	0.0	0.0	2.0080086687559692E-4	2.920739881826864E-4
14-15	0.0	0.0	0.0	2.1905549113701483E-4	3.468378609669401E-4
16-17	1.8254624261417904E-5	0.0	0.0	2.3731011539843274E-4	3.6509248522835806E-4
18-19	3.650924852283581E-5	0.0	0.0	2.5556473965985063E-4	3.6509248522835806E-4
20-21	3.650924852283581E-5	0.0	0.0	2.5556473965985063E-4	5.111294793197013E-4
22-23	3.650924852283581E-5	0.0	0.0	2.5556473965985063E-4	5.841479763653729E-4
24-25	3.650924852283581E-5	3.650924852283581E-5	0.0	4.0160173375119383E-4	6.206572248882087E-4
26-27	3.650924852283581E-5	3.650924852283581E-5	0.0	6.206572248882087E-4	6.206572248882087E-4
28-29	3.650924852283581E-5	3.650924852283581E-5	0.0	0.0020080086687559694	6.206572248882087E-4
30-31	5.4763872784253714E-5	3.650924852283581E-5	0.0	0.007812979183886862	6.206572248882087E-4
32-33	7.301849704567161E-5	3.650924852283581E-5	0.0	0.019952304317729767	7.849488432409698E-4
34-35	1.2778236982992531E-4	3.650924852283581E-5	0.0	0.03552349881271924	8.397127160252235E-4
36-37	1.8254624261417903E-4	3.650924852283581E-5	0.0	0.058469561509321546	8.762219645480593E-4
38-39	1.8254624261417903E-4	3.650924852283581E-5	0.0	0.09134613980413518	8.762219645480593E-4
40-41	1.8254624261417903E-4	3.650924852283581E-5	0.0	0.12599341665230637	8.762219645480593E-4
42-43	1.8254624261417903E-4	1.0952774556850741E-4	0.0	0.1608049851188303	8.944765888094773E-4
44-45	2.0080086687559692E-4	1.0952774556850741E-4	0.0	0.19955955242582052	9.492404615937309E-4
46-47	2.1905549113701483E-4	1.0952774556850741E-4	0.0	0.2397197258009399	0.0011135320799464921
48-49	2.1905549113701483E-4	1.0952774556850741E-4	0.0	0.2804823017766861	0.0011682959527307458
50-51	2.1905549113701483E-4	1.0952774556850741E-4	0.0	0.31724711503918174	0.0012048052012535815
52-53	2.1905549113701483E-4	1.0952774556850741E-4	0.0	0.35410320142298446	0.001296078322560671
54-55	2.1905549113701483E-4	1.0952774556850741E-4	0.0	0.39283951410571327	0.001314332946822089
56-57	2.1905549113701483E-4	1.0952774556850741E-4	0.0	0.43486165915549724	0.001314332946822089
58-59	2.1905549113701483E-4	1.0952774556850741E-4	0.0	0.4754964527614135	0.001314332946822089
60-61	2.1905549113701483E-4	1.0952774556850741E-4	0.0	0.5153462975240888	0.001314332946822089
62-63	2.1905549113701483E-4	1.0952774556850741E-4	0.0	0.5561453827483578	0.0016429161835276112
64-65	2.1905549113701483E-4	1.0952774556850741E-4	0.0	0.599919971727238	0.0016429161835276112
66-67	2.1905549113701483E-4	1.0952774556850741E-4	0.0	0.6497003320881245	0.0016429161835276112
68-69	2.1905549113701483E-4	1.0952774556850741E-4	0.0	0.6979655586353135	0.0016429161835276112
70-71	2.5556473965985063E-4	1.0952774556850741E-4	0.0	0.744642632871759	0.001697680056311865
72-73	2.9207398818268646E-4	1.0952774556850741E-4	0.0	0.7905895221377479	0.0019167355474488798
74-75	2.9207398818268646E-4	1.0952774556850741E-4	0.0	0.8394571512855636	0.0020080086687559694
76-77	2.9207398818268646E-4	1.0952774556850741E-4	0.0	0.8911359925696378	0.0020080086687559694
78-79	2.9207398818268646E-4	1.0952774556850741E-4	0.0	0.9468491058154852	0.0020810271658016407
80-81	2.9207398818268646E-4	1.0952774556850741E-4	0.0	1.003894806632416	0.0021175364143244764
82-83	3.468378609669401E-4	1.0952774556850741E-4	0.0	1.0594436282599107	0.0022270641598929842
84-85	4.0160173375119383E-4	1.0952774556850741E-4	0.0	1.1161424912158746	0.00226357340841582
86-87	4.0160173375119383E-4	1.0952774556850741E-4	0.0	1.1754700200654828	0.002318337281200074
88-89	4.0160173375119383E-4	1.0952774556850741E-4	0.0	1.2382294182762377	0.0023731011539843273
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9520	0.0	24.722332	1
GTACATG	7525	0.0	23.932318	1
TATACCG	280	1.8189894E-12	23.742676	5
TACATGG	7895	0.0	22.20366	2
TCAACGC	10880	0.0	21.177713	4
CAACGCA	10935	0.0	20.930902	5
TATCAAC	11145	0.0	20.929157	2
ACATGGG	8135	0.0	20.6734	3
ATCAACG	11305	0.0	20.465605	3
AACGCAG	11430	0.0	20.108269	6
CATGGGG	6510	0.0	18.901009	4
AGAGTAC	8950	0.0	18.676315	10-11
GAGTACT	4945	0.0	18.146284	12-13
ACGCAGA	12690	0.0	18.113028	7
GAGTACA	6360	0.0	17.828684	1
GTACCTG	2680	0.0	17.777254	1
GGTATCA	7150	0.0	17.724543	1
CGCAGAG	13305	0.0	17.240091	8
GTACTTT	5100	0.0	17.12148	14-15
AGTACAT	5910	0.0	16.880377	2
>>END_MODULE