FastQCFastQC Report
Mon 15 Aug 2016
SRR2921427_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921427_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4605213
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52630.11428353042519422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG63300.037.232551
TACCTGG64150.035.724522
GTATCAA91950.034.2922021
TCAACGC109600.028.0611254
CAACGCA112200.027.5334175
TATCAAC113400.027.2420582
ACCTGGG81500.027.0508273
ATCAACG113700.027.0492463
AACGCAG119400.025.9047416
GGTATCA60150.025.6369111
GTACATG126950.025.091691
TACATGG131800.023.5084692
ACGCAGA138500.021.9978857
ACATGGG135250.021.790483
CGCAGAG147150.020.7307288
GAGTACA100250.019.5605811
CCTGGGG79350.019.1771564
AGTACAT93200.019.0321622
CATGGGG101950.018.8823094
TATAACG3659.094947E-1218.8361232
Produced by FastQC (version 0.11.5)