##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921427_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4605213
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.47592326348423	31.0	31.0	34.0	27.0	34.0
2	30.57776068121062	31.0	31.0	34.0	27.0	34.0
3	30.714552182494057	31.0	31.0	34.0	27.0	34.0
4	34.21659019897668	37.0	35.0	37.0	30.0	37.0
5	34.208618146435356	37.0	35.0	37.0	30.0	37.0
6	34.30301660314083	37.0	35.0	37.0	31.0	37.0
7	34.19701325432722	37.0	35.0	37.0	30.0	37.0
8	34.17853593308279	37.0	35.0	37.0	30.0	37.0
9	35.648097492993266	39.0	37.0	39.0	30.0	39.0
10-11	35.529039156277896	39.0	37.0	39.0	30.0	39.0
12-13	35.37446943279279	39.0	35.0	39.0	30.0	39.0
14-15	36.41643079266909	40.0	37.0	41.0	30.0	41.0
16-17	36.271586569394294	40.0	36.0	41.0	29.0	41.0
18-19	36.09497877731171	40.0	36.0	41.0	28.5	41.0
20-21	35.97894527354109	39.0	36.0	41.0	27.5	41.0
22-23	36.02304562242833	40.0	36.0	41.0	27.5	41.0
24-25	35.89768757710013	39.0	36.0	41.0	27.0	41.0
26-27	35.63837123277468	39.0	36.0	41.0	27.0	41.0
28-29	35.408219880383385	39.0	35.5	40.5	26.5	41.0
30-31	35.153695931111116	39.0	35.0	40.0	25.0	41.0
32-33	34.887471328687724	38.5	35.0	40.0	24.5	41.0
34-35	34.46895746190241	38.0	34.0	40.0	22.5	41.0
36-37	34.088965917537365	38.0	34.0	40.0	19.0	41.0
38-39	33.86367905241299	38.0	33.0	40.0	18.5	41.0
40-41	33.60061619299694	38.0	33.0	40.0	17.0	41.0
42-43	33.315339377353446	38.0	33.0	40.0	15.0	41.0
44-45	32.997706295018276	38.0	32.0	40.0	13.0	41.0
46-47	32.711831678578164	38.0	32.0	40.0	9.0	41.0
48-49	32.503601353509595	37.0	31.0	40.0	8.5	41.0
50-51	31.741704563936565	36.0	30.5	39.0	8.0	40.0
52-53	32.22796241997927	37.0	31.5	39.0	7.5	40.0
54-55	32.65505764879931	37.0	32.0	40.0	7.0	41.0
56-57	32.50649133927139	37.0	32.0	40.0	2.0	41.0
58-59	32.18243151836842	37.0	31.5	40.0	2.0	41.0
60-61	31.73564241219679	36.0	31.0	39.0	2.0	41.0
62-63	31.255004274503698	35.5	30.5	39.0	2.0	40.0
64-65	30.72121995225845	35.0	30.0	38.5	2.0	40.0
66-67	30.24113010625133	35.0	29.0	38.0	2.0	40.0
68-69	29.725792053483737	34.5	29.0	37.0	2.0	39.5
70-71	29.210941817457737	34.0	28.0	37.0	2.0	39.0
72-73	28.660943912909133	34.0	27.5	36.0	2.0	39.0
74-75	28.06270166005351	34.0	26.0	35.5	2.0	38.0
76-77	27.47993491289111	33.0	26.0	35.0	2.0	37.0
78-79	26.92427396952106	33.0	25.0	35.0	2.0	36.5
80-81	26.347532567983283	32.5	24.0	35.0	2.0	36.0
82-83	25.72438712389633	32.0	22.0	34.5	2.0	35.5
84-85	25.109755943970452	31.5	19.5	34.0	2.0	35.0
86-87	24.494132518951893	31.0	17.0	34.0	2.0	35.0
88-89	23.846165421664537	31.0	8.5	34.0	2.0	35.0
90-91	23.10489330243791	30.0	2.0	34.0	2.0	35.0
92-93	22.28431171370358	29.5	2.0	34.0	2.0	35.0
94-95	21.38615716580319	29.0	2.0	34.0	2.0	35.0
96-97	20.493037129878683	29.0	2.0	33.5	2.0	34.5
98	19.867159456033846	27.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	137288.0
3	24035.0
4	18741.0
5	16079.0
6	14919.0
7	15512.0
8	17600.0
9	20642.0
10	22666.0
11	25324.0
12	27959.0
13	32768.0
14	27634.0
15	27024.0
16	30114.0
17	34560.0
18	38749.0
19	37353.0
20	38622.0
21	42096.0
22	45868.0
23	50609.0
24	56454.0
25	63691.0
26	71929.0
27	82550.0
28	95164.0
29	112245.0
30	132697.0
31	161808.0
32	201506.0
33	261505.0
34	353314.0
35	498273.0
36	684377.0
37	737835.0
38	330756.0
39	14947.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.28742981466489	16.88664072746159	12.90109568773445	24.92483377013907
2	16.25499244032156	18.542645211765507	38.85926744445499	26.343094903457942
3	20.41082826836052	22.174227532346986	27.408106404517156	30.006837794775333
4	13.88510708011095	14.551427264048087	34.4302220429937	37.13324361284727
5	15.688810960113846	35.176859760582694	32.61825545538245	16.516073823920998
6	35.21200427904725	32.75040042729629	16.785981796013395	15.251613497643069
7	31.559421400650482	29.748772932364147	19.24088543957948	19.45092022740589
8	28.111730091458455	33.42630708225831	18.840671939136875	19.621290887146362
9	26.925990419075323	16.637246593924377	18.93342391009272	37.50333907690757
10-11	26.8521106617592	24.774645345363542	25.97933849925148	22.393905493625784
12-13	26.887629845828453	21.829192639994822	26.56721416109629	24.71596335308043
14-15	23.921604696690192	23.481680918870808	24.174523948003163	28.422190436435834
16-17	24.492550104311135	26.448617372506078	24.7138230606881	24.345009462494684
18-19	24.396821432462936	25.7782622473568	26.11987993082475	23.705036389355513
20-21	24.85980459361915	25.090391860342294	26.148230914581323	23.90157263145724
22-23	24.620678763803884	24.747236016342015	25.980114947113652	24.651970272740446
24-25	24.400244125764537	24.933163672373837	26.165049139708756	24.501543062152866
26-27	24.42076180099516	25.130143511863835	26.096579238525024	24.352515448615986
28-29	24.44207461000973	25.23292262965117	26.021325467018947	24.303677293320153
30-31	24.59812212550553	25.156523944062258	25.913531642986996	24.33182228744521
32-33	24.420760863839444	25.193680701767722	25.82102707331948	24.564531361073353
34-35	24.62821524674824	25.211226160022616	25.95972913771319	24.200829455515954
36-37	24.651383847745766	25.146808323926784	25.830035985866186	24.371771842461268
38-39	24.564076669160293	25.231392356174325	25.840348640361356	24.364182334304026
40-41	24.71258291686044	25.174636828137785	25.82528038333491	24.28749987166686
42-43	24.61477701829261	25.074835694499814	25.80344573400782	24.506941553199756
44-45	24.55442915785241	25.197789184769796	25.776148353051386	24.471633304326403
46-47	24.675532079795268	25.17610822758252	25.720031909723602	24.428327782898606
48-49	24.68884140432122	25.320212486466914	25.536949206635768	24.453996902576097
50-51	24.5252084140013	25.51945621267327	25.46119515619956	24.494140217125867
52-53	24.66305348709049	25.434337602924924	25.471779746236884	24.430829163747703
54-55	24.60565397686078	25.280764494669782	25.60933065866801	24.504250869801425
56-57	24.548664966676164	25.41252900353605	25.61816984671591	24.42063618307188
58-59	24.636388611680292	25.31576584575933	25.600972650162802	24.446872892397575
60-61	24.69513819266629	25.281140508685944	25.656751389437133	24.36696990921063
62-63	24.522615797508003	25.419989575729574	25.624762948291117	24.432631678471306
64-65	24.585489147212243	25.23166274660798	25.642316148082887	24.540531958096885
66-67	24.567675574759537	25.340407901828126	25.573317709162797	24.51859881424954
68-69	24.52466860569785	25.35725253961518	25.616271616629493	24.501807238057474
70-71	24.512388832335652	25.169506034367906	25.67790683603321	24.640198297263233
72-73	24.645088482816472	25.25186239562942	25.536838956331852	24.566210165222255
74-75	24.51121356994124	25.458954112769433	25.487063027451185	24.542769289838144
76-77	24.61391102858876	25.25950904048856	25.451598967549373	24.67498096337331
78-79	24.579737697679054	25.20749128516967	25.61992125781542	24.592849759335852
80-81	24.560120932791676	25.390115808114093	25.488158809591283	24.561604449502948
82-83	24.742766211753974	25.255624491639566	25.425751691534394	24.575857605072066
84-85	24.623307456130927	25.310124523152766	25.428623998057766	24.63794402265854
86-87	24.659738627960394	25.313681349484213	25.53379273035838	24.49278729219701
88-89	24.589635602830413	25.137013606334275	25.48575270148697	24.787598089348343
90-91	24.624648242373574	25.169920286087837	25.487802935882215	24.71762853565637
92-93	24.640993872285826	25.322951446762925	25.50320849029654	24.53284619065471
94-95	24.766296416454146	25.123361587720417	25.472866995950888	24.637474999874552
96-97	24.70940477952658	25.0626317360818	25.50533805538863	24.722625429002985
98	24.670836205617753	25.17067913892549	25.334464795246113	24.824019860210644
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	666.0
1	510.5
2	599.0
3	838.0
4	668.5
5	574.0
6	867.5
7	1728.5
8	2525.5
9	2513.0
10	2130.5
11	1910.5
12	2074.0
13	2430.0
14	2827.5
15	3182.5
16	3491.0
17	3682.0
18	3727.0
19	3602.0
20	3412.0
21	3347.0
22	3346.0
23	3411.5
24	3750.0
25	4450.0
26	5506.0
27	7302.0
28	9646.5
29	12646.5
30	16867.0
31	22446.0
32	29480.0
33	37122.0
34	45776.0
35	55960.0
36	66911.0
37	78294.5
38	90570.0
39	102408.5
40	114574.5
41	128294.0
42	141842.5
43	155449.0
44	168733.5
45	181159.0
46	194377.0
47	208122.5
48	214935.0
49	214061.0
50	212948.0
51	211667.5
52	210403.0
53	209955.5
54	205354.5
55	195468.5
56	181852.5
57	167808.5
58	153736.0
59	137558.0
60	120372.0
61	101492.5
62	82471.5
63	64025.5
64	47409.5
65	35604.0
66	27213.5
67	20893.0
68	16313.5
69	12705.5
70	9582.0
71	7355.0
72	5917.5
73	4494.0
74	3442.0
75	2693.0
76	1884.5
77	1147.5
78	731.0
79	461.5
80	285.0
81	163.0
82	82.0
83	58.0
84	44.5
85	29.0
86	18.5
87	15.0
88	13.5
89	13.5
90	13.5
91	10.0
92	9.0
93	9.0
94	6.5
95	6.5
96	5.5
97	1.5
98	1.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4929847978801415
2	0.054373163629999305
3	0.061929817361325086
4	0.06017094106179236
5	0.07144077809213167
6	0.07065905529233936
7	0.04703365512083806
8	0.022713390238410253
9	0.01404929587404535
10-11	0.01745847586202853
12-13	0.012366420402270211
14-15	0.016915612806617197
16-17	0.015612741473629992
18-19	0.05861835272331595
20-21	0.0897895493650348
22-23	0.10691145013270831
24-25	0.07570768170766477
26-27	0.09195014432557191
28-29	0.09400216667502675
30-31	0.0625812530278187
32-33	0.03681697241799674
34-35	0.4167559676392818
36-37	0.6502305105105888
38-39	0.5981156571911006
40-41	0.5924590241537144
42-43	0.564892438199927
44-45	0.548801977237535
46-47	0.5383247202680962
48-49	0.527869177820874
50-51	0.5421356189170837
52-53	0.6257908157559704
54-55	0.595933347708347
56-57	0.5511905746813448
58-59	0.5282383246985536
60-61	0.5415818986005642
62-63	0.5540677488750249
64-65	0.5735348180420753
66-67	0.6012751201735946
68-69	0.5522980153143839
70-71	0.4994665827617528
72-73	0.4821601085552395
74-75	0.46160731327736637
76-77	0.4764817609956369
78-79	0.47005426241956666
80-81	0.46707937287591256
82-83	0.48782759885373383
84-85	0.4517597774522047
86-87	0.4547672387791835
88-89	0.483669267849283
90-91	0.4656896434540596
92-93	0.46042387181656963
94-95	0.471986854896831
96-97	0.4663085073372285
98	0.4597615788889678
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4605213.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.19192298603185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.29594708990608	41.00536189960393
2	14.228276802441886	15.70571915008632
3	4.894888417490345	8.104749136900395
4	2.1370024374677072	4.7178109579872025
5	1.1889390245847438	3.2809915539984504
6	0.6935089431424392	2.2965655308025106
7	0.4860497494726194	1.877821423819102
8	0.3306103775972246	1.4597617998983146
9	0.25046560869486495	1.2441310727162918
>10	1.3736675198139368	13.710684071741424
>50	0.08842968765398485	3.303957093500786
>100	0.03142394848434321	2.7778221542377217
>500	5.927950738103887E-4	0.20897755042023472
>1k	1.580785408762012E-4	0.19085190805081617
>5k	3.951963521878112E-5	0.11479469623637559
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5263	0.11428353042519422	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.34290444329068E-5
2	0.0	0.0	0.0	2.17145222164534E-5	4.34290444329068E-5
3	0.0	0.0	0.0	2.17145222164534E-5	4.34290444329068E-5
4	0.0	0.0	0.0	2.17145222164534E-5	4.34290444329068E-5
5	0.0	0.0	0.0	2.17145222164534E-5	4.34290444329068E-5
6	0.0	0.0	0.0	2.17145222164534E-5	4.34290444329068E-5
7	0.0	0.0	0.0	2.17145222164534E-5	4.34290444329068E-5
8	0.0	0.0	0.0	2.17145222164534E-5	4.34290444329068E-5
9	0.0	0.0	0.0	2.17145222164534E-5	4.34290444329068E-5
10-11	0.0	0.0	0.0	1.302871332987204E-4	4.34290444329068E-5
12-13	0.0	0.0	0.0	1.520016555151738E-4	2.6057426659744075E-4
14-15	0.0	0.0	0.0	1.520016555151738E-4	3.6914687767970777E-4
16-17	0.0	0.0	0.0	1.737161777316272E-4	4.777194887619747E-4
18-19	0.0	0.0	0.0	1.9543069994808058E-4	5.211485331948815E-4
20-21	0.0	0.0	0.0	2.0628796105630727E-4	7.165792331429621E-4
22-23	0.0	0.0	0.0	2.8228878881389415E-4	7.382937553594155E-4
24-25	0.0	0.0	0.0	3.365750943550277E-4	7.817227997923223E-4
26-27	0.0	0.0	0.0	4.3429044432906794E-4	8.034373220087757E-4
28-29	0.0	0.0	0.0	8.577236275499092E-4	8.251518442252291E-4
30-31	0.0	2.17145222164534E-5	0.0	0.002377740182701647	8.468663664416825E-4
32-33	0.0	2.17145222164534E-5	0.0	0.005504631381870936	8.468663664416825E-4
34-35	0.0	2.17145222164534E-5	0.0	0.009500103469698361	8.468663664416825E-4
36-37	0.0	2.17145222164534E-5	0.0	0.016285891662340048	8.685808886581359E-4
38-39	0.0	5.428630554113349E-5	0.0	0.026165999270826343	9.337244553074961E-4
40-41	0.0	8.68580888658136E-5	0.0	0.03721869107900112	9.337244553074961E-4
42-43	0.0	1.0857261108226699E-4	0.0	0.04879253142037079	9.554389775239494E-4
44-45	0.0	1.0857261108226699E-4	0.0	0.06052923067836385	9.988680219568562E-4
46-47	0.0	1.0857261108226699E-4	0.0	0.07309108178058213	0.0010205825441733096
48-49	0.0	1.0857261108226699E-4	0.0	0.08646722746591742	0.0010640115886062164
50-51	0.0	1.0857261108226699E-4	0.0	0.09940908270692364	0.0010857261108226698
52-53	0.0	1.0857261108226699E-4	0.0	0.11193836202581726	0.00115086967747203
54-55	0.0	1.1942987219049368E-4	0.0	0.12596594337764616	0.00115086967747203
56-57	0.0	1.3028713329872038E-4	0.0	0.14043867243491234	0.0011834414607967102
58-59	0.0	1.3028713329872038E-4	0.0	0.1560731284307588	0.0011942987219049368
60-61	0.0	1.3028713329872038E-4	0.0	0.17064357283799902	0.0012160132441213904
62-63	0.0	1.3028713329872038E-4	0.0	0.18671231927817455	0.0012594422885542971
64-65	0.0	1.3028713329872038E-4	0.0	0.20228163170737162	0.001368014899636564
66-67	0.0	1.411443944069471E-4	0.0	0.2184915225419541	0.0014114439440694709
68-69	0.0	1.520016555151738E-4	0.0	0.23604771375395667	0.0014223012051776977
70-71	0.0	1.520016555151738E-4	0.0	0.2536364767492839	0.0014331584662859243
72-73	0.0	1.737161777316272E-4	0.0	0.27055208955590115	0.001552588338476418
74-75	0.0	1.737161777316272E-4	0.0	0.28755456045138417	0.0016503036884504582
76-77	0.0	1.737161777316272E-4	0.0	0.30595761802982835	0.001693732732883365
78-79	0.0	1.737161777316272E-4	0.0	0.3248058233137099	0.0017045899939915916
80-81	0.0	1.737161777316272E-4	0.0	0.34470718292508945	0.0017263045162080452
82-83	0.0	1.737161777316272E-4	0.0	0.3650319757196898	0.0018891634328314455
84-85	0.0	1.737161777316272E-4	0.0	0.3863447792751389	0.0019325924772643523
86	0.0	1.737161777316272E-4	0.0	0.40254381284861307	0.0019760215216972593
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	6330	0.0	37.23255	1
TACCTGG	6415	0.0	35.72452	2
GTATCAA	9195	0.0	34.292202	1
TCAACGC	10960	0.0	28.061125	4
CAACGCA	11220	0.0	27.533417	5
TATCAAC	11340	0.0	27.242058	2
ACCTGGG	8150	0.0	27.050827	3
ATCAACG	11370	0.0	27.049246	3
AACGCAG	11940	0.0	25.904741	6
GGTATCA	6015	0.0	25.636911	1
GTACATG	12695	0.0	25.09169	1
TACATGG	13180	0.0	23.508469	2
ACGCAGA	13850	0.0	21.997885	7
ACATGGG	13525	0.0	21.79048	3
CGCAGAG	14715	0.0	20.730728	8
GAGTACA	10025	0.0	19.560581	1
CCTGGGG	7935	0.0	19.177156	4
AGTACAT	9320	0.0	19.032162	2
CATGGGG	10195	0.0	18.882309	4
TATAACG	365	9.094947E-12	18.836123	2
>>END_MODULE