FastQCFastQC Report
Mon 15 Aug 2016
SRR2921427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4605213
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55810.12118874849002641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG62100.035.8998371
GTATCAA93700.034.8756871
TACCTGG64950.033.7901462
TCAACGC115950.027.5734084
CAACGCA117650.027.1993835
TATCAAC120650.026.6556072
ATCAACG120500.026.5319633
GGTATCA62750.026.4941481
ACCTGGG80900.026.072093
AACGCAG127150.025.3926036
GTACATG130500.024.1284391
TACATGG131900.023.2656362
ACGCAGA147200.021.8058227
ACATGGG134650.021.450563
CGCAGAG156450.020.547148
GTATAGG12150.019.6034261
CCTGGGG80950.018.8966644
GTACACG11850.018.4917371
CATGGGG103300.018.3952544
AGAGTAC138450.018.24475910-11
Produced by FastQC (version 0.11.5)