##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921427_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4605213
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.955094368056375	33.0	31.0	34.0	30.0	34.0
2	32.06854883802335	34.0	31.0	34.0	30.0	34.0
3	32.19457818780586	34.0	31.0	34.0	30.0	34.0
4	35.724780808184114	37.0	35.0	37.0	35.0	37.0
5	35.52995138335621	37.0	35.0	37.0	33.0	37.0
6	35.564971261915574	37.0	35.0	37.0	33.0	37.0
7	35.49677376486169	37.0	35.0	37.0	33.0	37.0
8	35.45109813596027	37.0	35.0	37.0	33.0	37.0
9	37.08108723744157	39.0	37.0	39.0	34.0	39.0
10-11	36.99026527111775	39.0	37.0	39.0	33.5	39.0
12-13	36.870691431644964	39.0	37.0	39.0	33.0	39.0
14-15	38.17433069871035	40.0	38.0	41.0	33.0	41.0
16-17	38.07304287554126	40.0	38.0	41.0	33.0	41.0
18-19	37.97132923059151	40.0	38.0	41.0	33.0	41.0
20-21	37.86063717356831	40.0	38.0	41.0	33.0	41.0
22-23	37.82362857049175	40.0	38.0	41.0	33.0	41.0
24-25	37.87715009056041	40.0	38.0	41.0	33.0	41.0
26-27	37.763241135643455	40.0	38.0	41.0	33.0	41.0
28-29	37.63101760982608	40.0	38.0	41.0	32.0	41.0
30-31	37.49051878816463	40.0	37.5	41.0	32.0	41.0
32-33	37.32669216385865	40.0	37.0	41.0	32.0	41.0
34-35	37.17154635409915	40.0	37.0	41.0	31.0	41.0
36-37	37.06913893016458	40.0	37.0	41.0	31.0	41.0
38-39	37.03506461047513	40.0	37.0	41.0	31.0	41.0
40-41	36.871089567409804	40.0	37.0	41.0	31.0	41.0
42-43	36.634817651213964	40.0	36.0	41.0	30.0	41.0
44-45	36.42139907535221	39.0	36.0	41.0	30.0	41.0
46-47	36.1366696828138	39.0	35.0	40.5	29.5	41.0
48-49	35.79830498611031	39.0	35.0	40.0	28.5	41.0
50-51	35.52351324466426	39.0	35.0	40.0	28.0	41.0
52-53	35.21114039676341	38.0	34.5	40.0	27.0	41.0
54-55	34.88165237959677	38.0	34.0	40.0	26.5	41.0
56-57	34.500988770769126	38.0	34.0	40.0	26.0	41.0
58-59	34.10543779408249	37.0	33.0	40.0	25.0	41.0
60-61	33.67688769227395	36.5	33.0	39.5	24.0	41.0
62-63	33.23530920285337	36.0	32.5	39.0	22.5	40.0
64-65	32.715615868364836	35.5	32.0	39.0	22.0	40.0
66-67	32.18466843987456	35.0	31.0	38.0	20.0	40.0
68-69	31.67431072134991	35.0	31.0	37.5	18.5	39.5
70-71	31.117382952753758	34.5	30.0	37.0	17.0	39.0
72-73	30.563044098068865	34.0	30.0	36.0	13.0	39.0
74-75	30.03900449338608	34.0	29.0	36.0	9.0	38.5
76-77	28.756350249163283	32.5	28.0	34.5	7.5	36.0
78-79	29.68489492234127	34.0	29.5	35.0	7.5	37.0
80-81	29.707802006117852	34.0	30.0	35.0	7.0	36.5
82-83	29.364001078777463	34.0	29.0	35.0	2.0	36.0
84-85	28.86530590441745	34.0	29.0	35.0	2.0	36.0
86-87	28.335514231372144	33.0	28.5	35.0	2.0	35.0
88-89	27.78481527347378	33.0	27.0	35.0	2.0	35.0
90-91	27.301270104118963	33.0	26.0	35.0	2.0	35.0
92-93	26.855082707357944	32.5	25.0	34.0	2.0	35.0
94-95	26.34469035417037	32.0	25.0	34.0	2.0	35.0
96-97	25.564923164248864	32.0	23.5	34.0	2.0	35.0
98-99	24.54602295268427	31.5	10.5	34.0	2.0	35.0
100-101	20.95340584680882	27.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29398.0
3	15886.0
4	9351.0
5	7006.0
6	6249.0
7	6328.0
8	7421.0
9	9059.0
10	10545.0
11	12388.0
12	15372.0
13	17924.0
14	19698.0
15	22435.0
16	25324.0
17	30144.0
18	30959.0
19	26884.0
20	27195.0
21	28740.0
22	30684.0
23	34073.0
24	38352.0
25	45318.0
26	53162.0
27	63890.0
28	77349.0
29	94139.0
30	115229.0
31	143574.0
32	182956.0
33	245649.0
34	346997.0
35	521978.0
36	804415.0
37	993708.0
38	441045.0
39	14389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.55883118577727	17.262072419376516	13.336195724841835	24.84290067000438
2	16.338440806103865	18.78349600767652	38.587639703093	26.290423483126624
3	20.47782918862763	22.23522030961893	27.630217997976526	29.65673250377691
4	14.158753046374423	14.792820464682915	34.351804531405904	36.696621957536756
5	15.760780700586748	35.147204343266964	32.65085076707902	16.441164189067266
6	35.392381585205825	32.7910119332292	16.650830006838046	15.16577647472693
7	31.68233716455946	29.744464439266217	19.154551521411324	19.418646874763
8	28.12054855981203	33.48728582275603	18.776493982940814	19.615671634491125
9	26.873608147307408	16.56230261936733	18.976730983320156	37.58735825000511
10-11	26.857234706839243	24.79524783706075	25.95694466301098	22.390572793089028
12-13	26.87204350834148	21.759419964131396	26.694330687130886	24.674205840396237
14-15	23.87542831552531	23.419394980928082	24.284071122296137	28.421105581250472
16-17	24.45026118302688	26.42752992377116	24.859725789047527	24.262483104154438
18-19	24.392605079256118	25.701943985245784	26.30362577469409	23.60182516080401
20-21	24.85681152293372	24.949265806771958	26.30043634772889	23.893486322565426
22-23	24.554745768713495	24.64199897942093	26.21913905096574	24.58411620089984
24-25	24.36828403837275	24.829485157532698	26.35952725581967	24.442703548274878
26-27	24.367560206569905	25.05370850099989	26.304009174127614	24.27472211830259
28-29	24.339069649852433	25.173203715979355	26.229747469438212	24.257979164729992
30-31	24.49609245132692	25.103239674783893	26.081239749050013	24.31942812483917
32-33	24.33650826948119	25.104851286364344	26.06168611791444	24.496954326240026
34-35	24.4834181969959	25.10819601214983	26.191578550988886	24.216807239865386
36-37	24.513320893152162	25.099862586127987	26.071782053237968	24.315034467481883
38-39	24.468427165109574	25.20696996235725	26.02996325854916	24.29463961398401
40-41	24.592434816122058	25.121857165646578	26.02442279856485	24.26128521966651
42-43	24.473911392642833	25.060485274144355	25.992191392638492	24.473411940574323
44-45	24.437503956689735	25.116670089010995	25.980729685798387	24.465096268500883
46-47	24.53074373909592	25.11415177992069	25.985728923392198	24.369375557591194
48-49	24.567218988890836	25.24544950062122	25.76388879086894	24.42344271961901
50-51	24.469492370690343	25.462737508945622	25.661525518819005	24.406244601545026
52-53	24.57732629464367	25.355015294394708	25.620893360290292	24.446765050671335
54-55	24.498406740808846	25.245698938507623	25.749181437846385	24.506712882837142
56-57	24.49346536691697	25.37531428876684	25.720305825757666	24.410914518558524
58-59	24.597094706345327	25.268419857743908	25.726972144528332	24.407513291382433
60-61	24.640545585037017	25.282200841917362	25.695179007875197	24.38207456517042
62-63	24.471867978151852	25.38668112756337	25.726189334733963	24.41526155955081
64-65	24.547701743741378	25.196745832614898	25.711553236686107	24.543999186957617
66-67	24.50436064445607	25.313047958834332	25.648828877832443	24.533762518877154
68-69	24.481755463062026	25.34078138973797	25.70210656958033	24.475356577619674
70-71	24.495700296057652	25.130131263264367	25.747438338515565	24.62673010216242
72-73	24.66041843243579	25.225326983153312	25.60712409915908	24.507130485251817
74-75	24.43291155713423	25.444170714589177	25.51936284177141	24.603554886505187
76-77	24.528715127119384	25.239971596354575	25.608968494606792	24.62234478191925
78-79	24.48749312084976	25.208129267721585	25.722236075594868	24.582141535833784
80-81	24.4464631246215	25.40177745224026	25.62584877971218	24.52591064342607
82-83	24.57660573678687	25.29961477673482	25.57561669552049	24.548162790957814
84-85	24.510240318639575	25.37395007671592	25.506586253857648	24.609223350786852
86-87	24.4844724871913	25.358944720023878	25.62829321168754	24.528289581097276
88-89	24.453830548464918	25.188543752607366	25.639221553945006	24.718404144982706
90-91	24.492248638873793	25.286980446377427	25.586085219366872	24.634685695381904
92-93	24.54679932556779	25.379911244512527	25.664411534867714	24.40887789505197
94-95	24.587860049350276	25.27992573027821	25.55206345500791	24.580150765363598
96-97	24.461743658253855	25.25961126280738	25.66490233795632	24.613742740982445
98-99	24.45290379576291	25.227840024878574	25.616383112627222	24.702873066731296
100-101	24.903493391555802	24.746854740181366	25.73648179990471	24.613170068358123
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	263.0
1	224.0
2	396.0
3	591.5
4	436.5
5	353.5
6	667.0
7	1492.5
8	2216.5
9	2138.5
10	1590.0
11	1166.0
12	1099.5
13	1177.0
14	1271.0
15	1348.5
16	1440.0
17	1621.0
18	1765.0
19	1916.0
20	2100.5
21	2210.5
22	2424.0
23	2793.0
24	3193.5
25	3781.0
26	4678.5
27	6054.5
28	7989.0
29	10638.5
30	14386.0
31	18810.5
32	24564.0
33	31514.0
34	39223.0
35	47912.5
36	58415.0
37	70055.0
38	81078.0
39	92423.5
40	104768.0
41	116834.5
42	129241.5
43	142693.5
44	155828.0
45	169689.0
46	182280.5
47	194295.5
48	207815.0
49	214498.0
50	212163.0
51	209988.5
52	208996.5
53	206564.0
54	203016.5
55	194948.5
56	181692.0
57	166467.0
58	151765.0
59	136321.0
60	119812.5
61	102474.0
62	83681.5
63	64222.5
64	47227.0
65	35297.0
66	26941.5
67	21001.5
68	16327.0
69	12603.0
70	9803.5
71	7553.0
72	5640.5
73	4427.5
74	3511.5
75	2627.5
76	1877.5
77	1218.5
78	774.5
79	452.0
80	271.5
81	147.5
82	71.0
83	40.0
84	27.5
85	16.0
86	6.0
87	4.5
88	2.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31803089238217647
2	0.0
3	0.00568920482071079
4	0.06559957161590571
5	3.474323554632544E-4
6	0.06562128613812217
7	0.07526253400222747
8	0.10588001032742678
9	0.09404559571945967
10-11	0.09834507111831743
12-13	0.0984102146849668
14-15	0.06574071601031267
16-17	0.035622673696091796
18-19	0.03871699311193641
20-21	0.028576311236852673
22-23	0.0422130311887854
24-25	0.03434151688532105
26-27	0.020856798588903488
28-29	0.02382083087144938
30-31	0.003300607376900917
32-33	0.005178913548624135
34-35	0.008164660353386477
36-37	0.01108526359149946
38-39	0.01607960370128374
40-41	0.0206179388445225
42-43	0.0036154679490394905
44-45	0.01453787262391555
46-47	0.024873985198947363
48-49	0.003072604893628156
50-51	0.00667721558155942
52-53	0.002052022349454846
54-55	0.004060615654476785
56-57	0.003745755082338211
58-59	0.0014657302496106043
60-61	0.005385201509680443
62-63	0.013278430335361252
64-65	0.0062320678761221245
66-67	0.038326131712040246
68-69	0.06186467379467574
70-71	0.011628126646910795
72-73	0.031681487913805505
74-75	0.050757695680959815
76-77	0.3094970851511103
78-79	0.07481738629679018
80-81	0.0335489368244205
82-83	0.027349440731623053
84-85	0.019293352989318845
86-87	0.11747556519101288
88-89	0.5308332101034198
90-91	0.13003741629323118
92-93	0.04842338454269107
94-95	0.008153803092278252
96-97	0.005450345076329803
98-99	0.009706391430754668
100-101	0.007534939209109329
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4605213.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.26094769199278
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.63935352786281	36.21040206187961
2	15.95371138304658	16.356047293990013
3	5.67065117114664	8.720488591910566
4	2.589100720466416	5.308790264045192
5	1.3399205202268254	3.434279784938753
6	0.8418949058269753	2.589379843785107
7	0.5525336933027628	1.9826380525319556
8	0.38016061304395804	1.5589914639841795
9	0.27926810863782026	1.2883993118032555
>10	1.6073790479752788	15.016579460452888
>50	0.10597961270057317	3.712085748781872
>100	0.0390719262079602	3.2381663658718365
>500	7.628633267602472E-4	0.2553345046438684
>1k	1.695249829242271E-4	0.207169868966724
>5k	4.2381245731024944E-5	0.12124738241431297
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5581	0.12118874849002641	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.17145222164534E-5	0.0
3	0.0	0.0	0.0	2.17145222164534E-5	0.0
4	0.0	0.0	0.0	2.17145222164534E-5	0.0
5	0.0	0.0	0.0	2.17145222164534E-5	0.0
6	0.0	0.0	0.0	2.17145222164534E-5	2.17145222164534E-5
7	0.0	2.17145222164534E-5	0.0	2.17145222164534E-5	2.17145222164534E-5
8	0.0	2.17145222164534E-5	0.0	2.17145222164534E-5	2.17145222164534E-5
9	0.0	2.17145222164534E-5	0.0	2.17145222164534E-5	2.17145222164534E-5
10-11	0.0	2.17145222164534E-5	0.0	4.34290444329068E-5	5.428630554113349E-5
12-13	0.0	2.17145222164534E-5	0.0	6.514356664936019E-5	2.605742665974408E-4
14-15	0.0	2.17145222164534E-5	0.0	6.514356664936019E-5	4.1257592211261455E-4
16-17	0.0	2.17145222164534E-5	0.0	7.60008277575869E-5	4.777194887619747E-4
18-19	0.0	4.34290444329068E-5	0.0	1.0857261108226699E-4	5.211485331948815E-4
20-21	0.0	4.34290444329068E-5	0.0	1.1942987219049368E-4	5.428630554113349E-4
22-23	0.0	8.68580888658136E-5	0.0	2.1714522216453397E-4	5.862920998442417E-4
24-25	0.0	8.68580888658136E-5	0.0	2.7143152770566745E-4	5.862920998442417E-4
26-27	0.0	8.68580888658136E-5	0.0	3.040033110303476E-4	6.188638831689218E-4
28-29	0.0	8.68580888658136E-5	0.0	6.731501887100554E-4	6.731501887100554E-4
30-31	0.0	8.68580888658136E-5	0.0	0.0021388804383206594	6.731501887100554E-4
32-33	0.0	8.68580888658136E-5	0.0	0.005157199026407682	6.731501887100554E-4
34-35	0.0	9.771534997404029E-5	0.0	0.009272100986425601	6.948647109265087E-4
36-37	0.0	1.0857261108226699E-4	0.0	0.016296748923448276	6.948647109265087E-4
38-39	0.0	1.0857261108226699E-4	0.0	0.026046569398635852	7.274364942511888E-4
40-41	0.0	1.0857261108226699E-4	0.0	0.03703411764016127	7.382937553594155E-4
42-43	0.0	1.3028713329872038E-4	0.0	0.04818452479831009	7.382937553594155E-4
44-45	0.0	1.3028713329872038E-4	0.0	0.0597366506174633	7.708655386840956E-4
46-47	0.0	1.520016555151738E-4	0.0	0.07216821458638287	8.251518442252291E-4
48-49	0.0	1.520016555151738E-4	0.0	0.08542493039952767	9.228671941992694E-4
50-51	0.0	1.520016555151738E-4	0.0	0.09827992755166808	9.771534997404028E-4
52-53	0.0	1.520016555151738E-4	0.0	0.11062463343172184	0.001042297066389763
54-55	0.0	1.520016555151738E-4	0.0	0.12430478242808747	0.0010640115886062164
56-57	0.0	1.520016555151738E-4	0.0	0.13839750734656575	0.001074868849714443
58-59	0.0	1.520016555151738E-4	0.0	0.1537931035980312	0.0010857261108226698
60-61	0.0	1.520016555151738E-4	0.0	0.16812468826089044	0.0010857261108226698
62-63	0.0	1.520016555151738E-4	0.0	0.18382428782338622	0.0011074406330391232
64-65	0.0	1.520016555151738E-4	0.0	0.1990244533749036	0.0011291551552555766
66-67	0.0	1.520016555151738E-4	0.0	0.21489776911513103	0.0011291551552555766
68-69	0.0	1.520016555151738E-4	0.0	0.23200881262169631	0.0011291551552555766
70-71	0.0	1.520016555151738E-4	0.0	0.24922842873934387	0.00115086967747203
72-73	2.17145222164534E-5	2.1714522216453397E-4	0.0	0.26575318014606486	0.001226870505229617
74-75	2.17145222164534E-5	2.6057426659744075E-4	0.0	0.28236478964165174	0.0013354431163118841
76-77	2.17145222164534E-5	2.6057426659744075E-4	0.0	0.30031184225355045	0.0013571576385283373
78-79	2.17145222164534E-5	2.6057426659744075E-4	0.0	0.3188560442264017	0.0014657302496106045
80-81	3.25717833246801E-5	2.6057426659744075E-4	0.0	0.3382471125656946	0.0014983020329352845
82-83	4.34290444329068E-5	2.6057426659744075E-4	0.0	0.3579421842160178	0.0015634455995846446
84-85	4.34290444329068E-5	2.7143152770566745E-4	0.0	0.3788424118493542	0.0015960173829093248
86-87	4.34290444329068E-5	2.8228878881389415E-4	0.0	0.3998729266159893	0.0016611609495586848
88-89	4.34290444329068E-5	2.8228878881389415E-4	0.0	0.4222931708044775	0.0017371617773162718
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	6210	0.0	35.899837	1
GTATCAA	9370	0.0	34.875687	1
TACCTGG	6495	0.0	33.790146	2
TCAACGC	11595	0.0	27.573408	4
CAACGCA	11765	0.0	27.199383	5
TATCAAC	12065	0.0	26.655607	2
ATCAACG	12050	0.0	26.531963	3
GGTATCA	6275	0.0	26.494148	1
ACCTGGG	8090	0.0	26.07209	3
AACGCAG	12715	0.0	25.392603	6
GTACATG	13050	0.0	24.128439	1
TACATGG	13190	0.0	23.265636	2
ACGCAGA	14720	0.0	21.805822	7
ACATGGG	13465	0.0	21.45056	3
CGCAGAG	15645	0.0	20.54714	8
GTATAGG	1215	0.0	19.603426	1
CCTGGGG	8095	0.0	18.896664	4
GTACACG	1185	0.0	18.491737	1
CATGGGG	10330	0.0	18.395254	4
AGAGTAC	13845	0.0	18.244759	10-11
>>END_MODULE