FastQCFastQC Report
Mon 15 Aug 2016
SRR2921426_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921426_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4255922
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT153870.36154328016349924No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT66190.15552446684878155No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC55930.13141688217030292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55900.13134639215662317No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT54860.1289027383490581No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT47810.11233758513431404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG56950.039.123121
TACCTGG62450.034.8670922
ACCTGGG76000.028.2887553
GTACATG120400.024.8906571
TACATGG123600.023.291412
GAGTACA96200.021.3423211
ACATGGG128450.021.2342783
AGTACAT88550.021.121572
CCTGGGG75350.020.7453444
CGTATAC2954.7002686E-918.6472073
CATGGGG101000.018.4723664
GTATACG2503.024361E-718.4137331
AGAGTAC126250.017.72407310-11
TATCACG4105.638867E-1116.7711182
GTGTAGG15050.016.2114261
GTATAGG10300.016.089671
TATAACG3453.252171E-815.9447152
GAGTACT67650.015.68083812-13
CTTAGAC15050.015.5341793
GTATTAG8400.015.3447781
Produced by FastQC (version 0.11.5)