##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921426_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4255922
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.01038952311626	31.0	31.0	34.0	26.0	34.0
2	30.095044270078258	31.0	31.0	34.0	26.0	34.0
3	30.231011987531726	31.0	31.0	34.0	26.0	34.0
4	33.72381049276749	37.0	35.0	37.0	28.0	37.0
5	33.698869951094025	37.0	35.0	37.0	28.0	37.0
6	33.79939387986904	37.0	35.0	37.0	30.0	37.0
7	33.67241575386015	37.0	35.0	37.0	29.0	37.0
8	33.6474293936778	37.0	35.0	37.0	29.0	37.0
9	35.053370808957496	39.0	35.0	39.0	29.0	39.0
10-11	34.90778331933715	39.0	35.0	39.0	28.0	39.0
12-13	34.727349091454215	38.0	35.0	39.0	27.0	39.0
14-15	35.669767913979626	39.0	36.0	41.0	27.0	41.0
16-17	35.513015276125834	39.0	36.0	41.0	27.0	41.0
18-19	35.32045265397251	39.0	36.0	40.0	26.5	41.0
20-21	35.18698251518707	39.0	35.0	40.0	25.5	41.0
22-23	35.22071645110037	39.0	35.5	40.0	25.5	41.0
24-25	35.06536033790093	39.0	35.0	40.5	24.5	41.0
26-27	34.76608029940398	39.0	34.5	40.0	23.5	41.0
28-29	34.507589542289544	38.5	34.0	40.0	22.5	41.0
30-31	34.227150662065704	38.0	34.0	40.0	20.0	41.0
32-33	33.918768483069	38.0	33.5	40.0	18.0	41.0
34-35	33.45670808816515	38.0	33.0	40.0	13.5	41.0
36-37	33.040780117680725	38.0	32.5	40.0	9.0	41.0
38-39	32.78024961453711	38.0	32.0	40.0	9.0	41.0
40-41	32.49521267072094	38.0	31.0	40.0	8.5	41.0
42-43	32.17391719115153	37.0	31.0	40.0	7.5	41.0
44-45	31.818015579232892	37.0	30.0	40.0	4.0	41.0
46-47	31.484919953890135	37.0	30.0	40.0	2.0	40.5
48-49	31.218895576563668	37.0	30.0	39.0	2.0	40.0
50-51	30.49020036081488	35.5	28.5	38.5	2.0	39.5
52-53	31.044426096154957	36.5	30.0	39.0	2.0	40.0
54-55	31.454387086981388	37.0	31.0	39.5	2.0	40.5
56-57	31.266832780300014	36.5	30.5	39.5	2.0	41.0
58-59	30.908768417278324	36.0	30.0	39.0	2.0	40.5
60-61	30.412913817499476	35.0	29.0	39.0	2.0	40.0
62-63	29.91589319541101	35.0	28.0	38.5	2.0	40.0
64-65	29.347175535641867	34.5	28.0	38.0	2.0	40.0
66-67	28.817465874609546	34.0	26.5	37.5	2.0	40.0
68-69	28.270128188439543	34.0	26.0	37.0	2.0	39.0
70-71	27.713180833671295	34.0	25.5	36.0	2.0	39.0
72-73	27.131138329132913	33.0	24.5	36.0	2.0	38.5
74-75	26.509252284228893	33.0	22.5	35.0	2.0	37.5
76-77	25.900521203161148	32.0	20.5	35.0	2.0	37.0
78-79	25.294145193450444	32.0	18.5	35.0	2.0	36.0
80-81	24.659748110985117	31.0	14.5	34.5	2.0	36.0
82-83	23.985175715156434	31.0	7.0	34.0	2.0	35.0
84-85	23.325027925793755	30.5	2.0	34.0	2.0	35.0
86-87	22.6719215013809	30.0	2.0	34.0	2.0	35.0
88-89	21.962185632161493	29.0	2.0	34.0	2.0	35.0
90-91	21.161217945253696	29.0	2.0	34.0	2.0	35.0
92-93	20.296446457430378	28.0	2.0	33.5	2.0	35.0
94-95	19.352071537025353	26.5	2.0	33.0	2.0	34.0
96-97	18.43783297720212	25.0	2.0	32.5	2.0	34.0
98	17.816244752605897	24.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	162630.0
3	28018.0
4	21191.0
5	17983.0
6	17061.0
7	17924.0
8	20416.0
9	23625.0
10	26108.0
11	28507.0
12	31264.0
13	34470.0
14	30831.0
15	31100.0
16	34806.0
17	39605.0
18	44190.0
19	42508.0
20	44294.0
21	48182.0
22	52192.0
23	57367.0
24	62975.0
25	70436.0
26	78413.0
27	89641.0
28	102383.0
29	117083.0
30	137734.0
31	163559.0
32	200331.0
33	253825.0
34	330798.0
35	443238.0
36	565653.0
37	551683.0
38	223609.0
39	10289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.436825631299776	16.58387389872002	12.491664840644976	24.487635629335227
2	16.084036588631324	18.787348621931503	38.59264150894604	26.53597328049113
3	21.204497203354563	22.389127526561666	27.12605006453797	29.2803252055458
4	13.820147900294494	14.25285994330191	34.7207861182309	37.2062060381727
5	15.308969940309499	35.75215533356	32.541668841633715	16.39720588449679
6	35.09414069765008	32.94302510975067	16.620852475482884	15.341981717116356
7	31.680912283671137	29.91158784562077	19.017169964785587	19.39032990592251
8	28.214111380051953	33.14973596715177	18.4624872178677	20.173665434928576
9	26.83560468194226	16.983649674008678	18.93052662450462	37.25021901954444
10-11	27.03214094813876	24.752429991418758	25.691079207479866	22.524349852962622
12-13	27.427449692061273	21.74273251441935	26.303181939525196	24.52663585399418
14-15	24.003490774206202	23.415870849344564	24.362203427252176	28.218434949197064
16-17	24.359826845483177	26.555819136932296	24.7036237027717	24.38073031481283
18-19	24.335967879507717	26.107833014065644	26.00966642874406	23.546532677682578
20-21	24.93365019912289	24.95488589809663	25.967849904292372	24.143613998488103
22-23	24.493215570417895	24.737703651374037	26.04632466403617	24.7227561141719
24-25	24.603641517836778	24.894846787160212	26.088883621822774	24.41262807318024
26-27	24.607189661224123	24.85774540981939	26.371121886058752	24.16394304289774
28-29	24.263787817177942	25.567153308189493	25.98660793133884	24.182450943293723
30-31	24.560948342645677	25.09166379635649	25.844429580221544	24.502958280776287
32-33	24.41503775445577	25.217172298269663	25.977467790100057	24.39032215717451
34-35	24.78578363539628	25.074126636395487	26.047523154620126	24.0925665735881
36-37	24.70104983748236	24.94513374123559	26.17166659098907	24.182149830292985
38-39	24.508939687907763	25.243546178430705	26.084788141917954	24.16272599174358
40-41	24.61610058335481	25.291408554350035	25.85138804835831	24.24110281393684
42-43	24.367752973831138	25.293555287397684	25.688766534968966	24.649925203802212
44-45	24.597525671924554	24.999294200001394	25.78276172983488	24.620418398239174
46-47	24.928509546135572	25.247708177449635	25.71957883497519	24.1042034414396
48-49	24.795427747565256	25.28659247487784	25.57894821376608	24.33903156379083
50-51	24.15214783231223	25.557054196889347	25.92016925683309	24.37062871396533
52-53	24.59594124251895	25.725998286803637	25.367068753175598	24.310991717501818
54-55	24.354315454013964	25.22844081161473	25.73980975940605	24.677433974965258
56-57	24.609077881781122	25.270000955651057	25.655474445311892	24.46544671725593
58-59	24.995822180178298	25.204175812106172	25.46573390565036	24.33426810206517
60-61	24.670694513624547	25.239767372171805	25.94998929275107	24.13954882145258
62-63	24.353736449903316	25.41594897013772	25.877960265611016	24.35235431434795
64-65	24.305631862776025	25.634003710480442	25.66474715115115	24.39561727559238
66-67	24.543773945884695	25.3360312747525	25.49857196310812	24.621622816254686
68-69	24.352188137344136	25.344674527379702	25.650144915038503	24.652992420237666
70-71	24.674447758980715	25.176669663208955	25.590105129282264	24.55877744852807
72-73	24.739724927784984	24.99456395352269	25.81756132226371	24.448149796428616
74-75	24.25523477484995	25.77995386470166	25.55259382702863	24.412217533419767
76-77	24.583871991266896	25.235431683379623	25.447219864036285	24.7334764613172
78-79	24.5780128793328	25.218591960650492	25.635037413047744	24.56835774696896
80-81	24.728121254397347	25.270975817459796	25.487159240525305	24.513743687617552
82-83	24.69747753924788	25.159064529625148	25.66257211854026	24.48088581258671
84-85	24.549924212420475	25.37307268007634	25.595986350210843	24.48101675729234
86-87	24.507846823287245	25.41491762071766	25.586961311648448	24.49027424434665
88-89	24.455182916032157	25.267674800203043	25.38391708278736	24.89322520097744
90-91	24.590374448694476	24.96643303555832	25.493979663423904	24.949212852323296
92-93	24.811114382947686	25.37239237411609	25.463859548907497	24.35263369402873
94-95	24.799380358975366	25.097893036380302	25.506592184468325	24.59613442017601
96-97	24.403703292786275	25.128970806589823	25.859384405473733	24.60794149515017
98	24.6945714227715	25.446299379724707	25.175486592017098	24.683642605486693
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	766.0
1	569.0
2	670.5
3	915.0
4	683.0
5	599.0
6	896.0
7	1706.0
8	2472.5
9	2441.5
10	2027.5
11	1807.5
12	1888.5
13	2230.5
14	2642.5
15	3046.5
16	3466.0
17	3625.0
18	3693.0
19	3721.5
20	3709.0
21	3744.5
22	3903.0
23	4297.5
24	4863.0
25	5781.5
26	7263.0
27	9345.5
28	12060.5
29	15339.0
30	19329.0
31	24409.0
32	30555.5
33	36835.0
34	43829.0
35	52032.0
36	60499.5
37	69642.0
38	79486.0
39	89171.5
40	99041.0
41	109634.0
42	121984.5
43	135838.0
44	147406.0
45	159611.5
46	181774.5
47	204346.5
48	208155.5
49	198219.0
50	192453.0
51	190531.0
52	188571.5
53	188807.0
54	186378.0
55	179727.5
56	169561.0
57	158472.5
58	145984.0
59	130609.0
60	114770.0
61	97359.5
62	79359.0
63	61410.5
64	45216.5
65	33841.5
66	25765.5
67	19503.5
68	14830.0
69	11213.0
70	8319.5
71	6391.5
72	5030.0
73	3739.5
74	2865.0
75	2141.5
76	1421.0
77	923.0
78	604.0
79	354.5
80	201.0
81	132.5
82	79.0
83	43.5
84	30.0
85	20.0
86	18.0
87	15.5
88	13.5
89	13.0
90	10.5
91	7.0
92	7.0
93	7.0
94	6.0
95	6.5
96	9.5
97	8.5
98	3.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.48990559507434583
2	0.05289100693104808
3	0.0613733052438461
4	0.05911762480609373
5	0.07011406694013658
6	0.06750593643398539
7	0.04657040237109609
8	0.02253330770629725
9	0.013957022708592875
10-11	0.016717881577716885
12-13	0.012453235750091286
14-15	0.01657690155035736
16-17	0.015214094619215296
18-19	0.05728488445041992
20-21	0.08572760497020387
22-23	0.10304465166419874
24-25	0.07236974737788897
26-27	0.08868818554475387
28-29	0.09053267423604099
30-31	0.06125582188771317
32-33	0.03579717861370579
34-35	0.4127660234374596
36-37	0.6451715985396349
38-39	0.5910235196979644
40-41	0.5865591521649128
42-43	0.558163424987582
44-45	0.5436307338339378
46-47	0.5325403050149885
48-49	0.5218610679425046
50-51	0.5363937590961488
52-53	0.6203356170531321
54-55	0.5902716262187137
56-57	0.5452520041485722
58-59	0.5229066698120877
60-61	0.5359238256716171
62-63	0.5484475514353881
64-65	0.5668571933414193
66-67	0.594207318649167
68-69	0.5467557911070738
70-71	0.494393459278624
72-73	0.47714690259830894
74-75	0.457468440446042
76-77	0.47195413825723315
78-79	0.46600948043690654
80-81	0.46299015818429
82-83	0.4824806469667442
84-85	0.4483164870032862
86-87	0.4513123125846761
88-89	0.4786741862280371
90-91	0.46086370943828386
92-93	0.45652857359697846
94-95	0.46807718750484617
96-97	0.4626964497939577
98	0.45621136853541955
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4255922.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.49536726630272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.74787962255283	42.7940427891981
2	13.429830965800168	15.17446465474491
3	4.6408636372692245	7.86561886861063
4	2.024158223064645	4.574222488685754
5	1.0926088386355277	3.0863668808561315
6	0.682683713603021	2.3141080276035657
7	0.46540461010855605	1.8405243062859307
8	0.32092824900012723	1.450476743471491
9	0.24979200393268802	1.2700881902126648
>10	1.2481163146450756	12.455259377125087
>50	0.07072157859051435	2.6974914173056037
>100	0.02533912516920673	2.302660464651167
>500	6.692472445798269E-4	0.2622552029810744
>1k	7.94730720513016E-4	0.9984976897858087
>5k	1.6731173063428046E-4	0.5503150991558062
>10k+	4.1827932658570116E-5	0.3636077993262792
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	15387	0.36154328016349924	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	6619	0.15552446684878155	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	5593	0.13141688217030292	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5590	0.13134639215662317	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	5486	0.1289027383490581	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	4781	0.11233758513431404	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.699334245317466E-5	0.0
3	0.0	0.0	0.0	4.699334245317466E-5	0.0
4	0.0	0.0	0.0	4.699334245317466E-5	0.0
5	0.0	0.0	0.0	4.699334245317466E-5	0.0
6	0.0	0.0	0.0	4.699334245317466E-5	0.0
7	0.0	2.349667122658733E-5	0.0	4.699334245317466E-5	0.0
8	0.0	2.349667122658733E-5	0.0	4.699334245317466E-5	0.0
9	0.0	4.699334245317466E-5	0.0	4.699334245317466E-5	0.0
10-11	0.0	4.699334245317466E-5	0.0	4.699334245317466E-5	0.0
12-13	0.0	4.699334245317466E-5	0.0	1.5272836297281763E-4	1.292316917462303E-4
14-15	0.0	5.874167806646833E-5	0.0	1.76225034199405E-4	2.1147004103928596E-4
16-17	0.0	8.223834929305566E-5	0.0	1.8797336981269864E-4	2.8196005471904797E-4
18-19	0.0	9.398668490634932E-5	0.0	1.9972170542599229E-4	2.8196005471904797E-4
20-21	0.0	9.398668490634932E-5	0.0	2.2321837665257964E-4	3.7594673962539727E-4
22-23	0.0	9.398668490634932E-5	0.0	2.937083903323416E-4	3.994434108519846E-4
24-25	0.0	9.398668490634932E-5	0.0	3.289533971722226E-4	4.229400820785719E-4
26-27	0.0	9.398668490634932E-5	0.0	4.464367533051593E-4	4.5818508891845296E-4
28-29	0.0	1.0573502051964298E-4	0.0	9.281185134501996E-4	4.934300957583339E-4
30-31	0.0	1.1748335613293664E-4	0.0	0.0026081305061511936	4.934300957583339E-4
32-33	0.0	1.1748335613293664E-4	0.0	0.005838922799806951	4.934300957583339E-4
34-35	0.0	1.1748335613293664E-4	0.0	0.009751118559033742	5.404234382115086E-4
36-37	0.0	1.4098002735952398E-4	0.0	0.015143604605535533	5.404234382115086E-4
38-39	0.0	1.76225034199405E-4	0.0	0.022827016096629592	5.404234382115086E-4
40-41	0.0	1.9972170542599229E-4	0.0	0.030898122662962338	5.639201094380959E-4
42-43	0.0	2.349667122658733E-4	0.0	0.03941566598260025	5.639201094380959E-4
44-45	0.0	2.349667122658733E-4	0.0	0.048908321158141525	5.639201094380959E-4
46-47	0.0	2.349667122658733E-4	0.0	0.05887090975821455	5.639201094380959E-4
48-49	0.0	2.349667122658733E-4	0.0	0.06896273005003381	6.109134518912705E-4
50-51	0.0	2.349667122658733E-4	0.0	0.07905455034185307	6.344101231178579E-4
52-53	0.0	2.349667122658733E-4	0.0	0.0886294438666874	7.283968080242072E-4
54-55	1.1748335613293665E-5	2.349667122658733E-4	0.0	0.09879175417218643	7.871384860906756E-4
56-57	2.349667122658733E-5	2.349667122658733E-4	0.0	0.11026987806637434	7.988868217039693E-4
58-59	2.349667122658733E-5	2.349667122658733E-4	0.0	0.1225703854534928	7.988868217039693E-4
60-61	2.349667122658733E-5	2.8196005471904797E-4	0.0	0.13417774103942695	7.988868217039693E-4
62-63	2.349667122658733E-5	2.937083903323416E-4	0.0	0.1459378249883339	7.988868217039693E-4
64-65	2.349667122658733E-5	3.289533971722226E-4	0.0	0.15843805408087835	8.223834929305565E-4
66-67	2.349667122658733E-5	3.524500683988099E-4	0.0	0.1710087731871026	8.458801641571439E-4
68-69	2.349667122658733E-5	3.524500683988099E-4	0.0	0.18384970401243256	8.693768353837312E-4
70-71	2.349667122658733E-5	3.524500683988099E-4	0.0	0.1973132966252671	9.163701778369058E-4
72-73	2.349667122658733E-5	3.524500683988099E-4	0.0	0.2097312873685185	0.0010456018695831361
74-75	2.349667122658733E-5	3.524500683988099E-4	1.1748335613293665E-5	0.2232653699950328	0.0011160918832628982
76-77	2.349667122658733E-5	3.524500683988099E-4	2.349667122658733E-5	0.2366467242585743	0.0011748335613293664
78-79	2.349667122658733E-5	3.524500683988099E-4	2.349667122658733E-5	0.25211928226128205	0.0012100785681692474
80-81	2.349667122658733E-5	3.524500683988099E-4	2.349667122658733E-5	0.26880191883215904	0.0012453235750091284
82-83	2.349667122658733E-5	3.524500683988099E-4	2.349667122658733E-5	0.285002873642891	0.0012923169174623031
84-85	2.349667122658733E-5	3.524500683988099E-4	2.349667122658733E-5	0.30099235841258365	0.0012923169174623031
86	2.349667122658733E-5	3.524500683988099E-4	2.349667122658733E-5	0.31269370068342417	0.0012923169174623031
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	5695	0.0	39.12312	1
TACCTGG	6245	0.0	34.867092	2
ACCTGGG	7600	0.0	28.288755	3
GTACATG	12040	0.0	24.890657	1
TACATGG	12360	0.0	23.29141	2
GAGTACA	9620	0.0	21.342321	1
ACATGGG	12845	0.0	21.234278	3
AGTACAT	8855	0.0	21.12157	2
CCTGGGG	7535	0.0	20.745344	4
CGTATAC	295	4.7002686E-9	18.647207	3
CATGGGG	10100	0.0	18.472366	4
GTATACG	250	3.024361E-7	18.413733	1
AGAGTAC	12625	0.0	17.724073	10-11
TATCACG	410	5.638867E-11	16.771118	2
GTGTAGG	1505	0.0	16.211426	1
GTATAGG	1030	0.0	16.08967	1
TATAACG	345	3.252171E-8	15.944715	2
GAGTACT	6765	0.0	15.680838	12-13
CTTAGAC	1505	0.0	15.534179	3
GTATTAG	840	0.0	15.344778	1
>>END_MODULE