FastQCFastQC Report
Mon 15 Aug 2016
SRR2921426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4255922
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT162520.3818679007744973No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT63500.14920386228882954No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62070.14584383830342756No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT54850.1288792416778315No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC53700.12617712448677396No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT48440.11381787542158901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG57850.041.341881
TACCTGG64200.036.251852
ACCTGGG80350.028.4340363
GTACATG125200.024.6962031
TACATGG127450.023.3294032
ACATGGG127200.022.3302423
CCTGGGG78800.022.3030914
GAGTACA98450.020.0827051
CATGGGG96650.019.6092344
TATAACG3455.2750693E-1119.2742752
AGAGTAC134650.019.23281910-11
GTATACG2752.7470378E-819.0567931
AGTACAT93800.018.7356172
GTATAGC9900.018.2868211
GTGTAGC25300.018.26591
GTATAGG11750.018.2458671
ATAACGC4507.2759576E-1216.8883343
GAGTACT74350.016.79017412-13
GTACTTT74500.016.59279414-15
AGTACTT85300.016.16506812-13
Produced by FastQC (version 0.11.5)