##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921426_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4255922
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89880312656106	33.0	31.0	34.0	30.0	34.0
2	32.01138131760874	34.0	31.0	34.0	30.0	34.0
3	32.14000984980458	34.0	31.0	34.0	30.0	34.0
4	35.68068399749807	37.0	35.0	37.0	35.0	37.0
5	35.48924604351301	37.0	35.0	37.0	33.0	37.0
6	35.52920988683533	37.0	35.0	37.0	33.0	37.0
7	35.46369012402013	37.0	35.0	37.0	33.0	37.0
8	35.41644160771744	37.0	35.0	37.0	33.0	37.0
9	37.03288970051613	39.0	37.0	39.0	33.0	39.0
10-11	36.93612300695361	39.0	37.0	39.0	33.0	39.0
12-13	36.814023729758205	39.0	37.0	39.0	33.0	39.0
14-15	38.084178704402945	40.0	38.0	41.0	33.0	41.0
16-17	37.99468188091793	40.0	38.0	41.0	33.0	41.0
18-19	37.895128952081365	40.0	38.0	41.0	33.0	41.0
20-21	37.77456905930137	40.0	38.0	41.0	32.5	41.0
22-23	37.736908359692684	40.0	38.0	41.0	32.5	41.0
24-25	37.78570718636291	40.0	38.0	41.0	33.0	41.0
26-27	37.66933416542878	40.0	38.0	41.0	32.0	41.0
28-29	37.52921822815362	40.0	38.0	41.0	32.0	41.0
30-31	37.38367279757477	40.0	37.0	41.0	32.0	41.0
32-33	37.206014936363964	40.0	37.0	41.0	31.0	41.0
34-35	37.039873498621446	40.0	37.0	41.0	31.0	41.0
36-37	36.91989244633713	40.0	37.0	41.0	31.0	41.0
38-39	36.86760542133996	40.0	37.0	41.0	31.0	41.0
40-41	36.70078810184961	40.0	36.0	41.0	30.0	41.0
42-43	36.44572926853453	39.0	36.0	41.0	30.0	41.0
44-45	36.21240567848753	39.0	35.0	41.0	30.0	41.0
46-47	35.90432096734855	39.0	35.0	40.0	29.0	41.0
48-49	35.53200916276191	39.0	35.0	40.0	28.0	41.0
50-51	35.22787647423989	38.0	34.5	40.0	27.0	41.0
52-53	34.90980379809592	38.0	34.0	40.0	26.0	41.0
54-55	34.56757689638109	38.0	34.0	40.0	26.0	41.0
56-57	34.15351420914199	37.5	33.5	40.0	24.5	41.0
58-59	33.73459781452762	37.0	33.0	40.0	23.0	41.0
60-61	33.27206960089964	36.0	32.5	39.0	22.0	40.5
62-63	32.822114456045014	36.0	32.0	39.0	21.5	40.0
64-65	32.28675372809934	35.0	31.0	38.5	19.5	40.0
66-67	31.716997045528558	35.0	31.0	38.0	18.0	40.0
68-69	31.180425651597936	35.0	30.0	37.0	14.0	39.5
70-71	30.589168222537914	34.0	29.5	36.5	9.5	39.0
72-73	30.019609499422216	34.0	29.0	36.0	7.5	39.0
74-75	29.482286564462413	34.0	29.0	35.5	7.0	38.0
76-77	28.259385862804816	32.0	27.0	34.5	4.5	36.0
78-79	29.178552849417823	33.5	29.0	35.0	2.0	37.0
80-81	29.173001290907116	34.0	29.0	35.0	2.0	36.5
82-83	28.809195751237922	34.0	29.0	35.0	2.0	36.0
84-85	28.280193457492878	33.5	27.5	35.0	2.0	35.5
86-87	27.740642920617436	33.0	27.0	35.0	2.0	35.0
88-89	27.15339085161805	33.0	25.5	35.0	2.0	35.0
90-91	26.62431395594186	32.0	25.0	34.0	2.0	35.0
92-93	26.137690610871157	32.0	24.5	34.0	2.0	35.0
94-95	25.579053023058222	32.0	23.5	34.0	2.0	35.0
96-97	24.746196241378485	31.5	19.0	34.0	2.0	35.0
98-99	23.67244007291487	31.0	2.0	34.0	2.0	35.0
100-101	20.12466852071067	25.5	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26943.0
3	14306.0
4	8746.0
5	6559.0
6	5795.0
7	6149.0
8	7214.0
9	8824.0
10	10489.0
11	12578.0
12	15089.0
13	17921.0
14	20026.0
15	22921.0
16	26748.0
17	32089.0
18	32875.0
19	28860.0
20	28423.0
21	30495.0
22	32083.0
23	35057.0
24	39722.0
25	46038.0
26	54385.0
27	65521.0
28	78603.0
29	94830.0
30	115995.0
31	143380.0
32	181258.0
33	239473.0
34	332706.0
35	487042.0
36	725094.0
37	849928.0
38	359238.0
39	12519.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.36562345824664	17.1486228437257	13.045169292622047	24.440584405405616
2	16.294448065542554	19.107399054775907	38.29102131101087	26.307131568670666
3	21.323578135963377	22.40813733097161	27.371614086322026	28.896670446742984
4	14.012852367991904	14.521431271901033	34.68596998418141	36.77974637592565
5	15.357675395415962	35.68487778607476	32.606866987883635	16.350579830625637
6	35.20378413252552	33.08000605671872	16.39760195319774	15.318607857558014
7	31.742899825215975	29.88856195756226	19.005887350485352	19.362650866736413
8	28.27720993948825	33.21877515344335	18.353692872428557	20.150322034639835
9	26.70474990792382	17.00208423782795	18.944103761270807	37.34906209297742
10-11	27.062325064617532	24.797668535662446	25.71864257728321	22.421363822436817
12-13	27.451242105352286	21.695890161586707	26.37317420285608	24.47969353020493
14-15	23.88897477420815	23.430413787630748	24.47862876087078	28.20198267729032
16-17	24.30793900811261	26.541583128707202	24.839659417810694	24.310818445369495
18-19	24.202530764833313	26.102191054128056	26.176388178481165	23.51889000255747
20-21	24.849717721275024	24.89691303182863	26.184689258366124	24.068679988530224
22-23	24.365039776250658	24.70389365794352	26.272847073115805	24.658219492690016
24-25	24.511570969411572	24.863915884761823	26.27827867638659	24.346234469440013
26-27	24.46489307497349	24.814686162732105	26.61328797746809	24.10713278482631
28-29	24.196136321385183	25.572281277608155	26.212596569050383	24.01898583195628
30-31	24.439303986905344	25.026998563366266	26.069180705214695	24.4645167445137
32-33	24.29914294230666	25.154168050606433	26.1558095098897	24.390879497197208
34-35	24.653954093651695	25.02254100912755	26.283016380622414	24.040488516598337
36-37	24.52768514167889	24.984887028999633	26.376584570775762	24.110843258545717
38-39	24.360196233788038	25.25483637137871	26.3032637127413	24.081703682091955
40-41	24.470126610748004	25.27538163774593	26.097451980288362	24.157039771217704
42-43	24.21891530887106	25.35480992897986	25.865458485778525	24.560816276370556
44-45	24.449130864628074	24.977669134713924	26.001046689544687	24.57215331111331
46-47	24.708068211877183	25.333818892359016	25.938946240554543	24.019166655209258
48-49	24.599720969850058	25.32353105311853	25.808350344381946	24.268397632649467
50-51	24.061716109187508	25.488572157020783	26.117423686118222	24.332288047673487
52-53	24.50440209040845	25.714674425768575	25.5037984463155	24.277125037507467
54-55	24.321324569353532	25.246413501911412	25.76388949264678	24.66837243608828
56-57	24.549159459397856	25.287462465690723	25.735771151327857	24.427606923583564
58-59	24.981622452270365	25.19997955765827	25.55503013776846	24.263367852302906
60-61	24.574473097164283	25.287834923301	26.054243320332322	24.083448659202393
62-63	24.286486264823147	25.470340315886332	25.94494884199021	24.298224577300314
64-65	24.332141619708356	25.639325633192588	25.745408471799696	24.283124275299357
66-67	24.477787709063854	25.428464476259823	25.530398256324734	24.563349558351593
68-69	24.349310990217695	25.403095497869092	25.69446150659444	24.553132005318766
70-71	24.660000086948237	25.262375113253015	25.629519912967165	24.448104886831583
72-73	24.673401304925772	25.09879079479567	25.87066641141182	24.35714148886674
74-75	24.220022591857294	25.882228483371712	25.572513161931425	24.32523576283957
76-77	24.42588760958083	25.31609871681993	25.555415576608443	24.702598096990798
78-79	24.45958132234417	25.340685568495125	25.7782689718824	24.4214641372783
80-81	24.640174854982856	25.424591908279638	25.553514316733565	24.38171892000394
82-83	24.588114141713156	25.30697317114359	25.772420026330707	24.33249266081255
84-85	24.428684049450656	25.52769193655488	25.71289276741462	24.330731246579848
86-87	24.38216260232793	25.571029172833565	25.638497944707833	24.40831028013067
88-89	24.247519251402842	25.509980870254594	25.452624705955166	24.7898751723874
90-91	24.488236837960027	25.20397766594989	25.52622690991173	24.781558586178352
92-93	24.692453324522525	25.60729162516031	25.576813470756544	24.123441579560616
94-95	24.584987881172633	25.377596436292826	25.5963216195439	24.441094062990643
96-97	24.165071341410258	25.37629271866687	26.032759172895037	24.42587676702784
98-99	24.430037924037016	25.618329117005707	25.457583462079075	24.4940494968782
100-101	24.805711177865224	24.750536618412816	25.767329963721785	24.67642224000017
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	358.0
1	311.5
2	513.0
3	740.5
4	504.5
5	357.5
6	653.5
7	1397.5
8	2134.5
9	2132.0
10	1563.0
11	1132.0
12	1063.0
13	1202.0
14	1381.5
15	1482.5
16	1633.5
17	1872.5
18	2132.0
19	2330.0
20	2488.0
21	2740.0
22	3117.5
23	3661.0
24	4328.0
25	5214.5
26	6548.5
27	8234.0
28	10465.0
29	13511.0
30	17206.0
31	21534.5
32	26774.5
33	32678.0
34	38723.5
35	45570.5
36	54325.0
37	63472.0
38	72298.0
39	81540.5
40	91556.0
41	101116.5
42	111318.0
43	123563.5
44	136101.5
45	148540.5
46	159353.0
47	177941.0
48	207869.0
49	212624.0
50	194631.0
51	187158.5
52	186716.5
53	185845.5
54	183778.5
55	179437.0
56	169712.5
57	156409.0
58	143360.5
59	129607.5
60	113557.0
61	96863.5
62	79676.0
63	61233.0
64	44615.5
65	33055.0
66	25077.0
67	19242.0
68	14596.5
69	10954.0
70	8356.0
71	6293.5
72	4649.5
73	3592.5
74	2802.5
75	2082.0
76	1477.0
77	908.5
78	521.0
79	262.0
80	142.0
81	79.5
82	42.0
83	23.0
84	11.5
85	10.5
86	5.0
87	2.0
88	2.5
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	1.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3193902519830016
2	0.0
3	0.0064850812585381026
4	0.06339401896933262
5	5.639201094380959E-4
6	0.065649699407085
7	0.07624669813027589
8	0.10611096725926837
9	0.09417465827616202
10-11	0.09806335736416222
12-13	0.09748768891911083
14-15	0.06660131459176179
16-17	0.03802936238023159
18-19	0.040273294482370686
20-21	0.02965279908795321
22-23	0.04407975522107783
24-25	0.03684278048328893
26-27	0.022979744459602408
28-29	0.025023954856315506
30-31	0.003289533971722226
32-33	0.006050392840846237
34-35	0.008106351573172629
36-37	0.011078680483335926
38-39	0.016247948153185136
40-41	0.020500845645197444
42-43	0.0037007257181875045
44-45	0.014121499407178986
46-47	0.024260313041451417
48-49	0.0025728854993113124
50-51	0.00721347806656231
52-53	0.0024201571363384952
54-55	0.003970937437293259
56-57	0.003501004012761512
58-59	0.001163085225716073
60-61	0.0053454927040486174
62-63	0.014697167852230374
64-65	0.006708299635190683
66-67	0.03969762603731929
68-69	0.06298282722286733
70-71	0.012136030688532355
72-73	0.03173225449150619
74-75	0.05115225326028062
76-77	0.3106377419510978
78-79	0.07629369147272906
80-81	0.03348275649788694
82-83	0.02804327710893198
84-85	0.01906754870037562
86-87	0.11904588476950471
88-89	0.5339383569529705
90-91	0.13253297405356582
92-93	0.04829740770625025
94-95	0.00830607327859862
96-97	0.006085637847686118
98-99	0.010373780346538306
100-101	0.007930126538973223
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4255922.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.347186165169155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.05871304856478	36.4866496756199
2	15.776736571728039	16.201820596547048
3	5.569584016634832	8.579474020940978
4	2.51226937734325	5.159918536619897
5	1.3406027297798038	3.441808896976877
6	0.8444808116490649	2.601702806919461
7	0.5495100705400067	1.9751057124157072
8	0.39004442516646426	1.6022146969367064
9	0.28224335787870747	1.304316201679241
>10	1.5465083349787463	14.233042993470413
>50	0.09234114709710724	3.22328836805366
>100	0.0349952759211865	2.894904422560065
>500	7.333333142047048E-4	0.24200140017917107
>1k	0.0010083328476832968	1.120294507642805
>5k	1.833332450333036E-4	0.5509807157727822
>10k+	4.58333112583259E-5	0.382476447665268
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	16252	0.3818679007744973	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	6350	0.14920386228882954	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6207	0.14584383830342756	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	5485	0.1288792416778315	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	5370	0.12617712448677396	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	4844	0.11381787542158901	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.349667122658733E-5
2	0.0	0.0	0.0	0.0	2.349667122658733E-5
3	0.0	0.0	0.0	0.0	2.349667122658733E-5
4	0.0	0.0	0.0	0.0	2.349667122658733E-5
5	0.0	0.0	0.0	0.0	2.349667122658733E-5
6	0.0	0.0	0.0	0.0	2.349667122658733E-5
7	0.0	0.0	0.0	0.0	2.349667122658733E-5
8	0.0	0.0	0.0	0.0	4.699334245317466E-5
9	0.0	0.0	0.0	2.349667122658733E-5	4.699334245317466E-5
10-11	0.0	0.0	0.0	9.398668490634932E-5	4.699334245317466E-5
12-13	0.0	0.0	0.0	9.398668490634932E-5	1.292316917462303E-4
14-15	0.0	0.0	0.0	1.0573502051964298E-4	2.2321837665257964E-4
16-17	0.0	0.0	0.0	1.292316917462303E-4	2.584633834924606E-4
18-19	0.0	0.0	0.0	2.1147004103928596E-4	2.819600547190479E-4
20-21	0.0	0.0	0.0	2.4671504787916694E-4	3.524500683988099E-4
22-23	0.0	0.0	0.0	3.1720506155892894E-4	3.8769507523869095E-4
24-25	0.0	0.0	0.0	3.641984040121036E-4	3.994434108519846E-4
26-27	0.0	0.0	0.0	4.8168176014504025E-4	3.994434108519846E-4
28-29	0.0	0.0	0.0	9.398668490634933E-4	3.994434108519846E-4
30-31	0.0	0.0	0.0	0.0026786205198309557	3.994434108519846E-4
32-33	0.0	2.349667122658733E-5	0.0	0.0059799028271664756	3.994434108519846E-4
34-35	0.0	2.349667122658733E-5	0.0	0.009962588600073027	4.5818508891845296E-4
36-37	0.0	2.349667122658733E-5	0.0	0.015449061331481169	4.699334245317466E-4
38-39	0.0	2.349667122658733E-5	0.0	0.023144221158188517	4.699334245317466E-4
40-41	0.0	4.699334245317466E-5	0.0	0.03109784436838833	4.699334245317466E-4
42-43	0.0	9.398668490634932E-5	0.0	0.03948615599628001	4.699334245317466E-4
44-45	0.0	9.398668490634932E-5	0.0	0.04883783114446176	4.934300957583339E-4
46-47	0.0	1.1748335613293664E-4	0.0	0.058459718011749276	5.286751025982149E-4
48-49	0.0	1.1748335613293664E-4	0.0	0.0684223066118223	5.639201094380959E-4
50-51	0.0	1.292316917462303E-4	0.0	0.07819692184208263	5.639201094380959E-4
52-53	0.0	1.4098002735952398E-4	0.0	0.08767782868201061	6.109134518912705E-4
54-55	0.0	1.4098002735952398E-4	0.0	0.09773440396699	6.344101231178579E-4
56-57	0.0	1.4098002735952398E-4	0.0	0.10893056780645885	6.696551299577389E-4
58-59	0.0	1.4098002735952398E-4	0.0	0.12094911513885828	7.049001367976198E-4
60-61	0.0	1.4098002735952398E-4	0.0	0.13203954395780748	7.049001367976198E-4
62-63	0.0	1.4098002735952398E-4	0.0	0.1433531911534093	7.753901504773819E-4
64-65	0.0	1.4098002735952398E-4	0.0	0.15532474514335554	7.871384860906756E-4
66-67	0.0	1.4098002735952398E-4	0.0	0.16754301418118095	7.988868217039693E-4
68-69	0.0	1.4098002735952398E-4	0.0	0.1798435215682994	7.988868217039693E-4
70-71	0.0	1.5272836297281763E-4	0.0	0.1928841740990554	7.988868217039693E-4
72-73	0.0	1.644766985861113E-4	0.0	0.20497321144513456	8.811251709970249E-4
74-75	0.0	1.644766985861113E-4	0.0	0.21801386397589054	9.281185134501995E-4
76-77	0.0	1.644766985861113E-4	0.0	0.23099577482858002	9.516151846767868E-4
78-79	0.0	1.644766985861113E-4	0.0	0.24584567104378324	9.868601915166678E-4
80-81	0.0	1.644766985861113E-4	0.0	0.261799910806636	0.0010221051983565488
82-83	0.0	1.76225034199405E-4	0.0	0.2770962437751444	0.0011043435476496045
84-85	0.0	1.8797336981269864E-4	2.349667122658733E-5	0.2925335567710122	0.0011043435476496045
86-87	0.0	1.8797336981269864E-4	2.349667122658733E-5	0.3078181414039073	0.0011395885544894855
88-89	0.0	1.8797336981269864E-4	2.349667122658733E-5	0.3251586847691288	0.00118658189694266
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	5785	0.0	41.34188	1
TACCTGG	6420	0.0	36.25185	2
ACCTGGG	8035	0.0	28.434036	3
GTACATG	12520	0.0	24.696203	1
TACATGG	12745	0.0	23.329403	2
ACATGGG	12720	0.0	22.330242	3
CCTGGGG	7880	0.0	22.303091	4
GAGTACA	9845	0.0	20.082705	1
CATGGGG	9665	0.0	19.609234	4
TATAACG	345	5.2750693E-11	19.274275	2
AGAGTAC	13465	0.0	19.232819	10-11
GTATACG	275	2.7470378E-8	19.056793	1
AGTACAT	9380	0.0	18.735617	2
GTATAGC	990	0.0	18.286821	1
GTGTAGC	2530	0.0	18.2659	1
GTATAGG	1175	0.0	18.245867	1
ATAACGC	450	7.2759576E-12	16.888334	3
GAGTACT	7435	0.0	16.790174	12-13
GTACTTT	7450	0.0	16.592794	14-15
AGTACTT	8530	0.0	16.165068	12-13
>>END_MODULE