FastQCFastQC Report
Mon 15 Aug 2016
SRR2921425_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921425_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4182054
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT145010.3467434901605766No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT70380.16829050987863858No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69630.16649713274864456No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT56560.13524454729661547No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT50620.12104100042706287No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC49930.11939109346746839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45620.10908515289376944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG46450.026.064381
TACCTGG47650.024.7237322
GTACATG140500.023.2954251
TACATGG142950.022.1904322
TCGCGTA2007.6512333E-720.6111879
ACATGGG152650.020.3599513
CGCGTAA1302.2677341E-819.37850210-11
AGAGTAC150100.018.72124510-11
ACCTGGG60100.018.6868213
CATGGGG116000.017.8222434
GAGTACA113650.017.7411611
GTGTAGC20500.017.7398781
AGTACAT108300.016.8037362
GAGTACT74900.016.66952512-13
TATAACG3905.002221E-1016.4553552
GTACACG10500.016.2214551
AGTACTT84350.016.13280712-13
GTACTTT77450.015.9503714-15
GTATCAA278550.015.8156181
GTATATG13500.015.3446211
Produced by FastQC (version 0.11.5)