##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921425_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4182054 Sequences flagged as poor quality 0 Sequence length 98 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.390500218313775 31.0 31.0 34.0 27.0 34.0 2 30.497072491172997 31.0 31.0 34.0 27.0 34.0 3 30.627520113322305 31.0 31.0 34.0 27.0 34.0 4 34.142605762622864 37.0 35.0 37.0 30.0 37.0 5 34.131925125787475 37.0 35.0 37.0 30.0 37.0 6 34.23831112654212 37.0 35.0 37.0 30.0 37.0 7 34.128668113802455 37.0 35.0 37.0 30.0 37.0 8 34.11656544846145 37.0 35.0 37.0 30.0 37.0 9 35.56528394898775 39.0 37.0 39.0 30.0 39.0 10-11 35.44200397699312 39.0 35.0 39.0 30.0 39.0 12-13 35.28285239741046 39.0 35.0 39.0 30.0 39.0 14-15 36.28275065314795 40.0 36.0 41.0 29.0 41.0 16-17 36.14713786574731 40.0 36.0 41.0 29.0 41.0 18-19 35.98316293859429 39.0 36.0 41.0 27.5 41.0 20-21 35.86832953376499 39.0 36.0 41.0 27.0 41.0 22-23 35.91199766908797 39.0 36.0 41.0 27.5 41.0 24-25 35.77631768982418 39.0 36.0 41.0 27.0 41.0 26-27 35.50593679565113 39.0 35.5 40.5 27.0 41.0 28-29 35.27237261881363 39.0 35.0 40.0 25.5 41.0 30-31 35.01880654816988 39.0 35.0 40.0 25.0 41.0 32-33 34.72821250036465 38.0 34.5 40.0 24.0 41.0 34-35 34.29203628169316 38.0 34.0 40.0 22.0 41.0 36-37 33.90101610357016 38.0 33.0 40.0 18.5 41.0 38-39 33.660017063385595 38.0 33.0 40.0 17.5 41.0 40-41 33.39896794732923 38.0 33.0 40.0 15.5 41.0 42-43 33.09438555791006 38.0 32.5 40.0 14.5 41.0 44-45 32.75530277705644 37.5 32.0 40.0 10.0 41.0 46-47 32.42445889029649 37.0 31.0 40.0 8.5 41.0 48-49 32.18096538208258 37.0 31.0 40.0 8.0 41.0 50-51 31.426066114880392 36.0 30.5 39.0 7.0 40.0 52-53 31.952949674968327 36.5 31.5 39.0 6.5 40.0 54-55 32.387707212771524 37.0 32.0 40.0 2.0 40.5 56-57 32.21826045287794 37.0 31.5 40.0 2.0 41.0 58-59 31.88354514312823 36.5 31.0 39.5 2.0 41.0 60-61 31.406667034906768 36.0 31.0 39.0 2.0 40.0 62-63 30.92559278287655 35.0 30.0 39.0 2.0 40.0 64-65 30.38332850317093 35.0 29.0 38.0 2.0 40.0 66-67 29.87330974205498 35.0 29.0 38.0 2.0 40.0 68-69 29.345506179499356 34.0 28.0 37.0 2.0 39.0 70-71 28.804894795715214 34.0 27.5 36.5 2.0 39.0 72-73 28.246072145409887 34.0 26.0 36.0 2.0 39.0 74-75 27.644745141980472 33.0 26.0 35.0 2.0 37.5 76-77 27.05663819740252 33.0 25.5 35.0 2.0 37.0 78-79 26.474388661648078 32.5 24.0 35.0 2.0 36.5 80-81 25.870045197886014 32.0 22.0 35.0 2.0 36.0 82-83 25.224572781700093 31.5 19.5 34.0 2.0 35.5 84-85 24.588259859867904 31.0 17.5 34.0 2.0 35.0 86-87 23.961427446895712 31.0 9.5 34.0 2.0 35.0 88-89 23.274547507038406 30.0 4.5 34.0 2.0 35.0 90-91 22.49595653236424 29.5 2.0 34.0 2.0 35.0 92-93 21.650110687236463 29.0 2.0 34.0 2.0 35.0 94-95 20.70401781038695 29.0 2.0 33.5 2.0 34.5 96-97 19.778700968471476 27.0 2.0 33.0 2.0 34.0 98 19.13958762847156 26.0 2.0 33.0 2.0 34.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 127792.0 3 22105.0 4 16972.0 5 14227.0 6 13535.0 7 14118.0 8 16074.0 9 18748.0 10 21083.0 11 23112.0 12 26301.0 13 29712.0 14 25712.0 15 26171.0 16 29562.0 17 34854.0 18 39528.0 19 36578.0 20 37126.0 21 39967.0 22 43755.0 23 48825.0 24 54775.0 25 61334.0 26 69568.0 27 80035.0 28 92472.0 29 108761.0 30 128962.0 31 156547.0 32 195179.0 33 251123.0 34 337252.0 35 466344.0 36 615348.0 37 606972.0 38 241643.0 39 9882.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.63852061519591 17.406077891778605 14.09840375613933 23.85699773688616 2 15.558285643194825 19.12217447891745 39.024250196183516 26.295289681704215 3 20.73186780162587 22.622323310242386 27.703104931406685 28.94270395672505 4 13.921893038084471 15.025128453592215 35.23443453502491 35.818543973298404 5 15.536769331527827 35.802895718111245 32.36696133371396 16.29337361664697 6 34.21020580105969 33.43097719072353 16.698894244332497 15.659922763884278 7 30.77522818138147 30.57845822304646 19.242235900026987 19.40407769554508 8 27.933550008383023 32.70230651539116 18.831571543067096 20.53257193315872 9 26.766488260081406 17.192607366804896 19.334323418136247 36.70658095497745 10-11 26.649184170089622 24.965770419663624 25.720985572755044 22.664059837491703 12-13 27.119309426416464 22.111022347312613 26.34791335521299 24.421754871057928 14-15 24.053922710494632 23.638144718961822 24.577250522442924 27.730682048100626 16-17 24.0520300113169 26.801401267914045 24.938943251902014 24.207625468867036 18-19 23.994862608513397 26.284557224181633 26.425888127271023 23.294692040033947 20-21 24.802177344592614 25.051776433983147 26.267713392083696 23.878332829340543 22-23 24.398875788568585 24.725649705829266 26.345635410218442 24.529839095383704 24-25 24.47933031132378 25.03602027042064 26.31656152329191 24.168087894963673 26-27 24.3793644397954 24.915342448278327 26.65539215680408 24.049900955122194 28-29 24.06411979841006 25.62860429735102 26.254860030987874 24.052415873251036 30-31 24.394223653266064 25.133099599008375 26.11936816601661 24.353308581708955 32-33 24.24657483456192 25.23992693203658 26.163885275212255 24.349612958189244 34-35 24.656517466337927 25.095143500183404 26.195826857259835 24.052512176218837 36-37 24.56061550837608 25.038119044068612 26.379389467048288 24.021875980507023 38-39 24.402519112507946 25.294223641723658 26.232551671974562 24.07070557379384 40-41 24.50294094228026 25.33919284599643 26.043630973386822 24.114235238336484 42-43 24.35309196625916 25.3183029342803 25.76004246163488 24.568562637825657 44-45 24.51355917103526 25.04584606902044 25.89504282954015 24.545551930404148 46-47 24.822615658966207 25.30189178707676 25.86303483683959 24.012457717117446 48-49 24.721688321210873 25.29872212345554 25.68897271034598 24.2906168449876 50-51 24.127931999397937 25.59185789351439 25.967330781130755 24.31287932595692 52-53 24.54068729388156 25.749329898873295 25.411779654072962 24.298203153172178 54-55 24.38794794887265 25.28613108623963 25.71471426825595 24.611206696631765 56-57 24.571710461375282 25.282515576815584 25.713230773966018 24.432543187843116 58-59 24.957258443643592 25.186487573845856 25.532230493196028 24.32402348931452 60-61 24.620000497709636 25.326627958321744 25.994039746802848 24.059331797165772 62-63 24.285375227751967 25.448007385211447 25.86900435509132 24.397613031945273 64-65 24.29309461562328 25.593382991363157 25.756631559706282 24.356890833307276 66-67 24.53494751608535 25.334953867346925 25.54093682792255 24.58916178864517 68-69 24.382351678737386 25.392699711550705 25.659374938756674 24.56557367095523 70-71 24.61120532358138 25.188821498132093 25.6856721098402 24.514301068446322 72-73 24.63793851884327 25.083313278713483 25.8729346494931 24.405813552950143 74-75 24.213772790282555 25.82051023325429 25.589634370472876 24.37608260599028 76-77 24.510611457851518 25.268112764385386 25.45626565588 24.76501012188309 78-79 24.51214323007205 25.27357436789875 25.697502012839408 24.516780389189798 80-81 24.666055764008334 25.33783645796268 25.53608750483825 24.460020273190736 82-83 24.65487386838893 25.143607051551708 25.730675634124545 24.470843445934815 84-85 24.503668809057377 25.39846425298387 25.60635342360121 24.49151351435754 86-87 24.57861689914216 25.412242313270283 25.547684521992064 24.461456265595494 88-89 24.328331148414335 25.323085059972033 25.35890163154636 24.989682160067275 90-91 24.536344130817707 25.08095740746013 25.48288239003253 24.899816071689635 92-93 24.781250439192828 25.38967590241889 25.520175482535528 24.30889817585275 94-95 24.79753089445369 25.125198432577854 25.572063672380036 24.50520700058842 96-97 24.36926143316699 25.15088317153958 25.896082245068076 24.58377315022535 98 24.619128305622763 25.444211170666797 25.204066507290946 24.732594016419498 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 787.0 1 581.5 2 632.5 3 859.5 4 674.0 5 567.5 6 895.5 7 1836.5 8 2762.5 9 2889.0 10 2393.0 11 1962.0 12 2030.5 13 2411.5 14 2891.0 15 3326.0 16 3680.5 17 3869.5 18 3932.0 19 3968.0 20 3923.0 21 3778.5 22 3787.5 23 4050.0 24 4404.5 25 5010.5 26 6057.0 27 7585.0 28 9719.0 29 12579.5 30 16071.5 31 20462.0 32 26560.5 33 33563.5 34 41409.5 35 50432.5 36 59945.0 37 70356.5 38 81133.5 39 91436.0 40 101823.0 41 113469.0 42 126068.0 43 138181.0 44 150395.0 45 164323.5 46 187663.0 47 210985.5 48 214738.5 49 202517.5 50 194036.0 51 192412.0 52 189800.5 53 187867.0 54 183357.5 55 174289.0 56 162441.0 57 150356.0 58 137519.0 59 122002.5 60 105600.0 61 88625.5 62 71418.0 63 54787.0 64 40082.5 65 29536.5 66 22247.5 67 16871.0 68 12587.5 69 9454.5 70 7040.5 71 5201.5 72 4007.5 73 2966.0 74 2225.0 75 1619.0 76 1058.0 77 650.0 78 376.0 79 221.0 80 139.0 81 87.0 82 44.0 83 33.5 84 25.5 85 18.0 86 18.0 87 15.5 88 11.5 89 6.5 90 8.0 91 8.5 92 7.0 93 6.0 94 4.0 95 4.5 96 6.0 97 4.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.501499980631527 2 0.05485342848275034 3 0.06307905158565623 4 0.06047267682339826 5 0.07142423316389505 6 0.06984606128950033 7 0.048923328106236785 8 0.023553019640588094 9 0.014203546869552618 10-11 0.01743162570354185 12-13 0.013019917963756565 14-15 0.017599007569007955 16-17 0.016690363156477653 18-19 0.05980314936153383 20-21 0.08975254743243392 22-23 0.10875038916283722 24-25 0.07548922132521484 26-27 0.09319583152202243 28-29 0.0948935618717501 30-31 0.06372466735245408 32-33 0.03736202354154203 34-35 0.4229141947951892 36-37 0.6607877373175957 38-39 0.606209293328111 40-41 0.5990596965032016 42-43 0.5726253176070897 44-45 0.557154450899008 46-47 0.5467409076975094 48-49 0.5360762916978117 50-51 0.5507341607736295 52-53 0.6342457557936841 54-55 0.6030051261891883 56-57 0.5592825917599342 58-59 0.537068627043075 60-61 0.550028765769165 62-63 0.5612792182979942 64-65 0.5806716029969963 66-67 0.6073450988437739 68-69 0.5604303531231304 70-71 0.5080278733847052 72-73 0.49024952810269784 74-75 0.46972133788803305 76-77 0.4849052642553157 78-79 0.4787838703182694 80-81 0.4760101136905453 82-83 0.497016537806542 84-85 0.460515335287397 86-87 0.46371950242631965 88-89 0.49192334675735894 90-91 0.4742765157982178 92-93 0.4683942388118374 94-95 0.4796925147307997 96-97 0.4737026351166197 98 0.46778449058763943 >>END_MODULE >>Sequence Length Distribution pass #Length Count 98 4182054.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.17233495860698 #Duplication Level Percentage of deduplicated Percentage of total 1 74.08460643181945 39.392515084692725 2 14.129708332917016 15.026191686905674 3 4.82669075865867 7.699392532830348 4 2.205085882169984 4.68998260556951 5 1.2385024589698899 3.292703379770269 6 0.7477768827797014 2.385662573127916 7 0.5298644525834519 1.9721891108798364 8 0.36761479112869877 1.5637549447706809 9 0.27194577898465266 1.3013992845676128 >10 1.4723961129731833 14.069914476261774 >50 0.09006657511455422 3.260445211649346 >100 0.03375661875434781 2.953237435061666 >500 8.120140954166485E-4 0.2790413158190648 >1k 9.473496180007868E-4 1.1727985146149453 >5k 1.8044754628585428E-4 0.5929357266432033 >10k+ 4.511188657146357E-5 0.347836116835353 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14501 0.3467434901605766 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7038 0.16829050987863858 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6963 0.16649713274864456 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5656 0.13524454729661547 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5062 0.12104100042706287 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4993 0.11939109346746839 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4562 0.10908515289376944 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 7.173508519976069E-5 5 0.0 7.173508519976069E-5 0.0 0.0 7.173508519976069E-5 6 4.782339013317379E-5 7.173508519976069E-5 0.0 0.0 7.173508519976069E-5 7 4.782339013317379E-5 7.173508519976069E-5 0.0 0.0 7.173508519976069E-5 8 4.782339013317379E-5 7.173508519976069E-5 0.0 0.0 7.173508519976069E-5 9 4.782339013317379E-5 7.173508519976069E-5 0.0 2.3911695066586896E-5 7.173508519976069E-5 10-11 9.564678026634758E-5 7.173508519976069E-5 0.0 9.564678026634758E-5 7.173508519976069E-5 12-13 1.1955847533293448E-4 7.173508519976069E-5 0.0 1.1955847533293448E-4 2.0324940806598862E-4 14-15 1.3151432286622792E-4 7.173508519976069E-5 0.0 1.4347017039952138E-4 2.1520525559928208E-4 16-17 1.7933771299940171E-4 7.173508519976069E-5 0.0 1.4347017039952138E-4 2.1520525559928208E-4 18-19 2.1520525559928208E-4 7.173508519976069E-5 0.0 1.4347017039952138E-4 2.1520525559928208E-4 20-21 2.1520525559928208E-4 7.173508519976069E-5 0.0 2.0324940806598862E-4 2.1520525559928208E-4 22-23 2.3911695066586896E-4 7.173508519976069E-5 0.0 2.2716110313257553E-4 2.1520525559928208E-4 24-25 2.510727981991624E-4 7.173508519976069E-5 0.0 3.1085203586562966E-4 2.1520525559928208E-4 26-27 2.6302864573245584E-4 7.173508519976069E-5 0.0 4.3041051119856416E-4 2.510727981991624E-4 28-29 2.749844932657493E-4 7.173508519976069E-5 0.0 8.847327174637152E-4 2.749844932657493E-4 30-31 3.586754259988035E-4 7.173508519976069E-5 0.0 0.0017933771299940174 2.988961883323362E-4 32-33 3.586754259988035E-4 7.173508519976069E-5 0.0 0.0038497829057204903 3.228078833989231E-4 34-35 3.8258712106539034E-4 7.173508519976069E-5 0.0 0.006671362923577744 3.586754259988035E-4 36-37 4.0649881613197725E-4 8.369093273305413E-5 0.0 0.010270073031099072 3.586754259988035E-4 38-39 4.0649881613197725E-4 9.564678026634758E-5 0.0 0.016331687730478852 3.586754259988035E-4 40-41 4.0649881613197725E-4 9.564678026634758E-5 0.0 0.02348128455538833 3.586754259988035E-4 42-43 4.0649881613197725E-4 9.564678026634758E-5 0.0 0.030977600958763327 3.586754259988035E-4 44-45 4.184546636652707E-4 9.564678026634758E-5 0.0 0.03929887084193556 3.945429685986838E-4 46-47 4.3041051119856416E-4 9.564678026634758E-5 0.0 0.047859257675773675 4.3041051119856416E-4 48-49 4.423663587318576E-4 9.564678026634758E-5 0.0 0.056455512052211665 4.5432220626515107E-4 50-51 4.5432220626515107E-4 1.1955847533293448E-4 0.0 0.06507567812371624 4.5432220626515107E-4 52-53 4.782339013317379E-4 1.1955847533293448E-4 0.0 0.07395887284095327 5.260572914649118E-4 54-55 4.782339013317379E-4 1.3151432286622792E-4 0.0 0.08413329909178599 5.499689865314986E-4 56-57 4.782339013317379E-4 1.4347017039952138E-4 0.0 0.09447510720808483 5.619248340647921E-4 58-59 4.782339013317379E-4 1.4347017039952138E-4 0.0 0.10437454896565182 5.738806815980855E-4 60-61 5.021455963983249E-4 1.4347017039952138E-4 0.0 0.11573260412228059 5.738806815980855E-4 62-63 5.021455963983249E-4 1.5542601793281483E-4 0.0 0.12695914495604313 5.738806815980855E-4 64-65 5.021455963983249E-4 1.673818654661083E-4 0.0 0.13767158434587407 5.738806815980855E-4 66-67 5.021455963983249E-4 1.673818654661083E-4 0.0 0.15010566578049925 6.097482241979659E-4 68-69 5.141014439316183E-4 1.673818654661083E-4 2.3911695066586896E-5 0.16282668755592347 6.217040717312593E-4 70-71 5.260572914649117E-4 1.673818654661083E-4 2.3911695066586896E-5 0.17582269382461346 7.053950044643135E-4 72-73 5.260572914649117E-4 1.673818654661083E-4 2.3911695066586896E-5 0.18789809983323985 7.412625470641938E-4 74-75 5.260572914649117E-4 1.673818654661083E-4 2.3911695066586896E-5 0.20096584118712957 7.651742421307807E-4 76-77 5.380131389982051E-4 1.9129356053269517E-4 2.3911695066586896E-5 0.21411727347375237 7.651742421307807E-4 78-79 5.499689865314986E-4 2.1520525559928208E-4 2.3911695066586896E-5 0.2291935972132354 7.651742421307807E-4 80-81 5.499689865314986E-4 2.1520525559928208E-4 4.782339013317379E-5 0.24554919663878083 8.24953479797248E-4 82-83 5.499689865314986E-4 2.1520525559928208E-4 4.782339013317379E-5 0.26048205020786436 9.564678026634758E-4 84-85 5.499689865314986E-4 2.1520525559928208E-4 4.782339013317379E-5 0.2760605195437457 9.684236501967694E-4 86 5.499689865314986E-4 2.1520525559928208E-4 4.782339013317379E-5 0.2883033074178382 9.803794977300629E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 4645 0.0 26.06438 1 TACCTGG 4765 0.0 24.723732 2 GTACATG 14050 0.0 23.295425 1 TACATGG 14295 0.0 22.190432 2 TCGCGTA 200 7.6512333E-7 20.611187 9 ACATGGG 15265 0.0 20.359951 3 CGCGTAA 130 2.2677341E-8 19.378502 10-11 AGAGTAC 15010 0.0 18.721245 10-11 ACCTGGG 6010 0.0 18.686821 3 CATGGGG 11600 0.0 17.822243 4 GAGTACA 11365 0.0 17.741161 1 GTGTAGC 2050 0.0 17.739878 1 AGTACAT 10830 0.0 16.803736 2 GAGTACT 7490 0.0 16.669525 12-13 TATAACG 390 5.002221E-10 16.455355 2 GTACACG 1050 0.0 16.221455 1 AGTACTT 8435 0.0 16.132807 12-13 GTACTTT 7745 0.0 15.95037 14-15 GTATCAA 27855 0.0 15.815618 1 GTATATG 1350 0.0 15.344621 1 >>END_MODULE