FastQCFastQC Report
Mon 15 Aug 2016
SRR2921425_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921425_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4182054
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT151000.36106659550546216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73420.175559665178881No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT67500.16140394169946157No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT56000.13390549237288663No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT53070.12689936571837665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49190.11762162803254095No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC46570.11135676392509519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG47350.027.9706171
TACCTGG50850.024.7540072
GTACATG139050.024.5654891
TACATGG141950.023.8251252
TATCACG4050.022.2837962
ACATGGG149250.021.3867873
AGAGTAC157550.019.32932310-11
ACCTGGG62000.019.076483
CATGGGG113300.018.9914844
GAGTACA110350.018.2186991
GAGTACT82450.017.6732312-13
GTATACG2453.6618367E-617.500631
GTACTTT82750.017.1742414-15
AGTACAT104500.017.1362462
AGTACTT92100.016.74911912-13
TACTTTT92100.016.04896714-15
GTATCAA298000.016.0187531
TAGCCCG3603.5732228E-815.8231785
GTGTAGC21550.015.6959761
GTACCCG10450.015.5002911
Produced by FastQC (version 0.11.5)