FastQCFastQC Report
Mon 15 Aug 2016
SRR2921424_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921424_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6538324
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90650.13864409288985985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72350.041.6736831
GTATCAA129250.041.4554441
TCAACGC166850.031.4357264
TATCAAC172300.030.6808722
CAACGCA170400.030.592475
ATCAACG175650.029.886913
AACGCAG179700.029.1544916
GTACCTG67000.028.4436071
GTACATG248100.027.6080231
TACCTGG67550.026.6062952
TACATGG254300.026.2505682
ACATGGG261550.024.5237393
ACGCAGA215450.023.9703887
CGCAGAG225700.022.8767158
GAGTACA189050.022.2550531
AGTACAT177800.021.4801332
GCAGAGT251500.020.1473759
CATGGGG204300.020.1077444
ACCTGGG93650.018.7995363
AGAGTAC217300.018.72195610-11
Produced by FastQC (version 0.11.5)