##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921424_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6538324
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.507095090423785	31.0	30.0	34.0	25.0	34.0
2	29.58464462758346	31.0	30.0	34.0	25.0	34.0
3	29.710067289415452	31.0	30.0	34.0	25.0	34.0
4	33.18017507238858	37.0	35.0	37.0	27.0	37.0
5	33.126622510600576	37.0	35.0	37.0	27.0	37.0
6	33.223544749388374	37.0	35.0	37.0	27.0	37.0
7	33.080983597631445	37.0	35.0	37.0	26.0	37.0
8	33.04413944613329	37.0	35.0	37.0	26.0	37.0
9	34.38991873146696	38.0	35.0	39.0	27.0	39.0
10-11	34.22815640521944	38.0	35.0	39.0	26.0	39.0
12-13	34.02503080300089	38.0	35.0	39.0	25.0	39.0
14-15	34.89932832940062	39.0	35.0	40.0	24.5	41.0
16-17	34.71453227157296	39.0	34.5	40.0	23.5	41.0
18-19	34.50798323851801	38.5	34.0	40.0	21.0	41.0
20-21	34.369276973732106	39.0	34.0	40.0	19.0	41.0
22-23	34.3827721752547	39.0	34.0	40.0	18.5	41.0
24-25	34.20270056974846	39.0	34.0	40.0	18.0	41.0
26-27	33.873952789736336	38.0	33.5	40.0	16.5	41.0
28-29	33.584519748486	38.0	33.0	40.0	13.0	41.0
30-31	33.278111714867606	38.0	33.0	40.0	9.5	41.0
32-33	32.94809365519359	38.0	32.0	40.0	9.0	41.0
34-35	32.47597786527557	38.0	31.5	40.0	5.0	41.0
36-37	32.03754914868092	37.5	30.5	40.0	2.0	41.0
38-39	31.74791162077621	37.0	30.0	40.0	2.0	41.0
40-41	31.423438101262647	37.0	30.0	40.0	2.0	41.0
42-43	31.08442369940676	37.0	30.0	40.0	2.0	41.0
44-45	30.710917736716624	36.0	29.0	39.0	2.0	40.0
46-47	30.35298617810925	36.0	28.0	39.0	2.0	40.0
48-49	30.074401100343145	36.0	27.5	39.0	2.0	40.0
50-51	29.399135695936756	34.5	26.5	38.5	2.0	39.5
52-53	29.99486863911914	35.5	28.5	38.5	2.0	40.0
54-55	30.38262672513629	36.0	29.0	39.0	2.0	40.0
56-57	30.195428981494338	36.0	28.0	39.0	2.0	40.0
58-59	29.822880756597563	35.0	28.0	39.0	2.0	40.0
60-61	29.32667339520036	35.0	27.0	39.0	2.0	40.0
62-63	28.800233821389092	34.5	26.0	38.0	2.0	40.0
64-65	28.215436631772914	34.0	25.5	38.0	2.0	40.0
66-67	27.685547014800736	34.0	24.5	37.0	2.0	39.5
68-69	27.132283288500233	33.5	22.5	36.5	2.0	39.0
70-71	26.57105980064616	33.0	21.5	36.0	2.0	39.0
72-73	25.979050824033806	32.5	19.0	35.5	2.0	38.5
74-75	25.34134894508134	32.0	16.0	35.0	2.0	37.5
76-77	24.707902208578222	31.5	9.0	35.0	2.0	37.0
78-79	24.09276276305671	31.0	4.5	34.5	2.0	36.0
80-81	23.456101288342396	31.0	2.0	34.0	2.0	36.0
82-83	22.771763528390455	30.0	2.0	34.0	2.0	35.0
84-85	22.097570187711714	29.5	2.0	34.0	2.0	35.0
86-87	21.415300388907006	29.0	2.0	34.0	2.0	35.0
88-89	20.701517086029998	28.5	2.0	34.0	2.0	35.0
90-91	19.907266831683472	27.0	2.0	33.5	2.0	35.0
92-93	19.04307915912396	25.5	2.0	33.0	2.0	34.5
94-95	18.104408255081886	24.5	2.0	32.5	2.0	34.0
96-97	17.20914029344523	21.5	2.0	32.0	2.0	34.0
98	16.60367381610333	18.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	322418.0
3	52057.0
4	38994.0
5	32742.0
6	31062.0
7	33287.0
8	37746.0
9	43104.0
10	47541.0
11	51893.0
12	55074.0
13	58838.0
14	52781.0
15	54436.0
16	59682.0
17	68315.0
18	75051.0
19	73523.0
20	77389.0
21	82755.0
22	90197.0
23	98018.0
24	107947.0
25	118332.0
26	130945.0
27	146532.0
28	165539.0
29	188590.0
30	218056.0
31	256960.0
32	309303.0
33	383715.0
34	491948.0
35	641071.0
36	791674.0
37	742511.0
38	294736.0
39	13562.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.79164780426249	16.82251391976279	12.806458364258456	24.579379911716266
2	16.290273244379346	18.17644582617141	38.948857859029474	26.58442307041977
3	20.714231388358	21.801254944233285	27.157041566068056	30.327472101340657
4	13.625847814649761	13.931261555544197	34.14909111943516	38.293799510370874
5	15.536902839739518	36.03705205806506	32.35135941720679	16.074685684988637
6	36.33949571292318	32.584186606297834	15.96587173418777	15.110445946591215
7	32.403388886508715	30.167944818131186	18.22701622528108	19.201650070079015
8	28.834475863077554	33.83992488246823	17.913949550830694	19.41164970362352
9	27.35801002951585	16.056678512166112	18.30479950757965	38.280511950738386
10-11	27.25444894961629	24.797900173336046	25.297670606136187	22.649980270911477
12-13	27.1103292697365	21.67972352979507	26.43105422544626	24.77889297502217
14-15	24.100598618100587	23.221008530042152	23.794845782433484	28.883547069423777
16-17	24.61846959225385	26.585218241198298	24.35063328422351	24.44567888232434
18-19	24.666528531599237	25.561501450647757	26.11519454625614	23.656775471496864
20-21	25.151308827673642	24.736672355369365	25.98239568726024	24.12962312969676
22-23	24.84231692745534	24.516482593679573	25.931054480740713	24.710145998124375
24-25	24.445690625665538	24.77606044380258	26.0791428980514	24.699106032480486
26-27	24.650851479086143	24.921518502927555	26.021507022310665	24.406122995675634
28-29	24.569155476921996	25.09795730489708	25.926099695791617	24.406787522389312
30-31	24.701768846169767	24.985452372118413	25.900424519101904	24.412354262609913
32-33	24.4670200805474	25.00283232418955	25.824615601397856	24.705531993865197
34-35	24.679392758563953	25.024374772404233	25.96630550006499	24.32992696896682
36-37	24.756918957647315	25.082930227801597	25.746164286995494	24.413986527555597
38-39	24.796320051646887	24.93268992125065	25.84048670761019	24.430503319492278
40-41	24.77630537781506	24.99424935223981	25.781251742402052	24.448193527543083
42-43	24.775607459656012	24.79672024490831	25.87675530781649	24.55091698761919
44-45	24.776354362006664	25.024011588906838	25.746696225226014	24.452937823860484
46-47	24.84819294296845	25.08521011320404	25.59826177672099	24.468335167106524
48-49	24.84613998216127	25.13367327606647	25.429099881907753	24.591086859864507
50-51	24.702643634036324	25.309655412987393	25.34430029107232	24.643400661903964
52-53	24.743749011430783	25.305794846577136	25.49045342169578	24.460002720296302
54-55	24.64902536184958	25.066557127611567	25.609919857650503	24.674497652888352
56-57	24.73508091292591	25.211874537493813	25.53028789967723	24.52275664990305
58-59	24.697424325338023	25.197010827794415	25.598564594772405	24.507000252095157
60-61	24.762286544470623	25.01661522851224	25.585583992805066	24.635514234212074
62-63	24.549340247037193	25.189889046369522	25.62126775770096	24.639502948892325
64-65	24.625394562812755	24.990817146688133	25.708882827560664	24.67490546293845
66-67	24.609624695320633	25.22068703961603	25.576768970840387	24.592919294222952
68-69	24.720903245100136	25.155143869834674	25.533963751651417	24.589989133413773
70-71	24.567801336948875	25.013233256693525	25.666025256791258	24.752940149566342
72-73	24.726067692604584	25.086691134342104	25.460743270557	24.72649790249631
74-75	24.557230932111608	25.22801308936153	25.519669527724258	24.6950864508026
76-77	24.73639643192706	25.021918471811805	25.542085117493997	24.69959997876714
78-79	24.730527395395683	24.9040204043633	25.602854791897496	24.76259740834352
80-81	24.58972358502443	25.130199021824183	25.44400860207257	24.83606879107882
82-83	24.706953874521027	25.231326527672405	25.340260638246793	24.72145895955978
84-85	24.793858185859875	25.062239605893655	25.376064232747865	24.767837975498608
86-87	24.745356901254237	25.056166640962985	25.49001611651571	24.708460341267067
88-89	24.773890838406402	24.860641529504107	25.474219057479708	24.891248574609783
90-91	24.775325881168055	24.88778583924732	25.390180212454922	24.94670806712971
92-93	24.733688643007024	25.094956073766916	25.506817074557965	24.664538208668098
94-95	24.794428873842172	24.82562384958445	25.519541144260288	24.860406132313095
96-97	24.84012380841772	24.946769385621927	25.409391816034997	24.803714989925357
98	24.87921566181645	24.899431513655763	25.387991507498835	24.833361317028952
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	779.0
1	602.0
2	714.0
3	1037.0
4	843.5
5	692.0
6	1205.0
7	2521.5
8	3795.0
9	3917.0
10	3128.0
11	2604.0
12	2804.5
13	3255.5
14	3751.5
15	4299.0
16	4728.5
17	5064.0
18	5408.0
19	5585.5
20	5525.0
21	5430.5
22	5733.0
23	6568.0
24	7550.5
25	8837.5
26	11171.5
27	14517.5
28	18879.5
29	24575.0
30	31130.0
31	38387.5
32	46638.0
33	55121.5
34	65540.0
35	78326.5
36	91277.5
37	105169.0
38	119565.5
39	133732.0
40	148502.5
41	164826.5
42	182419.0
43	203091.0
44	223600.0
45	240355.5
46	258578.5
47	277809.5
48	288750.5
49	291140.0
50	291673.0
51	291417.0
52	293949.0
53	297688.0
54	295890.5
55	285690.0
56	268889.5
57	252433.5
58	234900.0
59	213669.5
60	188425.5
61	158543.0
62	129180.5
63	100102.5
64	74828.5
65	57050.5
66	43620.5
67	33504.0
68	25868.0
69	20079.5
70	15457.0
71	12012.0
72	9627.0
73	7520.5
74	5827.0
75	4443.5
76	3066.5
77	2003.5
78	1274.0
79	827.5
80	545.0
81	311.5
82	188.0
83	117.5
84	64.0
85	42.5
86	30.0
87	22.0
88	17.0
89	17.0
90	16.5
91	12.0
92	11.5
93	9.5
94	9.0
95	8.5
96	6.5
97	6.5
98	5.5
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.46908045548064004
2	0.04871278939373454
3	0.05645177571499975
4	0.05478468182366
5	0.06536843386776182
6	0.0652766672315413
7	0.04349738556853408
8	0.020922793058282214
9	0.012541440283473258
10-11	0.015638564255916348
12-13	0.011715540557488434
14-15	0.015562092059065901
16-17	0.014307948030718576
18-19	0.05593176477641671
20-21	0.08401235545990074
22-23	0.10140978024337735
24-25	0.07013265173154466
26-27	0.08525120504887797
28-29	0.08612298809297307
30-31	0.05740767817563033
32-33	0.03322716953151909
34-35	0.3969748210703538
36-37	0.620074808161847
38-39	0.5697866915129932
40-41	0.56409716006732
42-43	0.5376836632751758
44-45	0.5226568765940629
46-47	0.5126849021247647
48-49	0.5019099695885367
50-51	0.5162638009373656
52-53	0.5969725574933271
54-55	0.5680660670838582
56-57	0.5254710534381594
58-59	0.5023305666712141
60-61	0.5161720343011451
62-63	0.5280558136917045
64-65	0.5462485493224258
66-67	0.5725091017208692
68-69	0.5267634335649319
70-71	0.47480822302473846
72-73	0.4568907873026788
74-75	0.437573910378256
76-77	0.45186656396960445
78-79	0.4454964299719622
80-81	0.44296520025621244
82-83	0.4628326769979585
84-85	0.42824430236250144
86-87	0.4317926122963622
88-89	0.45911612823102677
90-91	0.4419481200381015
92-93	0.4374744965223503
94-95	0.44756117928692435
96-97	0.44219283106802293
98	0.43713037163652335
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	6538324.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.326388620907075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.82671120700488	35.92278658979291
2	13.373586139690902	13.193414143631395
3	5.266242833226609	7.7929422169140725
4	2.679925049402403	5.287640978469061
5	1.4968479390457732	3.6917051573887814
6	0.9421596455622473	2.7883999691963686
7	0.689727929553115	2.3815251527081394
8	0.4593203435685467	1.812529101468055
9	0.340253219914708	1.510511629952506
>10	1.758478149497498	15.437466658629726
>50	0.11170469853546988	3.766091693305855
>100	0.05129122794330352	4.555161366133263
>500	0.0028450044240136926	0.9213699668772715
>1k	8.128251175465201E-4	0.6311986372585338
>5k	9.378751315292395E-5	0.30725673827424493
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9065	0.13864409288985985	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.529443937008934E-5	0.0
3	0.0	0.0	0.0	4.5883318110268014E-5	0.0
4	0.0	0.0	0.0	9.176663622053603E-5	0.0
5	0.0	4.5883318110268014E-5	0.0	9.176663622053603E-5	0.0
6	0.0	4.5883318110268014E-5	0.0	9.176663622053603E-5	3.058887874017868E-5
7	0.0	6.117775748035736E-5	0.0	9.176663622053603E-5	3.058887874017868E-5
8	0.0	6.117775748035736E-5	0.0	1.0706107559062536E-4	4.5883318110268014E-5
9	0.0	6.117775748035736E-5	0.0	1.0706107559062536E-4	4.5883318110268014E-5
10-11	0.0	6.117775748035736E-5	0.0	1.0706107559062536E-4	4.5883318110268014E-5
12-13	0.0	6.117775748035736E-5	0.0	1.3764995433080405E-4	1.5294439370089338E-4
14-15	0.0	7.647219685044669E-5	0.0	1.6059161338593804E-4	2.1412215118125073E-4
16-17	0.0	1.0706107559062536E-4	0.0	2.447110299214294E-4	2.1412215118125073E-4
18-19	0.0	1.3764995433080405E-4	0.0	3.0588878740178677E-4	2.752999086616081E-4
20-21	0.0	1.8353327244107205E-4	0.0	3.4412488582701007E-4	3.517721055120548E-4
22-23	0.0	1.8353327244107205E-4	0.0	3.5177210551205475E-4	4.2059708267745677E-4
24-25	0.0	2.0647493149620607E-4	0.0	4.0530264330736746E-4	4.741276204727695E-4
26-27	0.0	2.2176937086629538E-4	0.0	5.200109385830375E-4	4.8177484015781417E-4
28-29	0.0	2.2941659055134006E-4	0.0	8.106052866147349E-4	4.894220598428588E-4
30-31	0.0	2.447110299214294E-4	0.0	0.0018659216031508994	4.894220598428588E-4
32-33	0.0	2.447110299214294E-4	0.0	0.003777726524412066	5.047164992129481E-4
34-35	0.0	3.0588878740178677E-4	0.0	0.006171306285831048	5.200109385830375E-4
36-37	0.0	3.441248858270101E-4	0.0	0.009857266174022578	5.200109385830375E-4
38-39	0.0	3.976554236223228E-4	0.0	0.015546797619695811	5.200109385830375E-4
40-41	0.0	4.664804007877248E-4	0.0	0.021825164981117487	5.505998173232162E-4
42-43	7.64721968504467E-6	4.894220598428588E-4	0.0	0.028050001804743846	5.582470370082608E-4
44-45	1.529443937008934E-5	4.894220598428588E-4	0.0	0.03435131082522065	5.658942566933056E-4
46-47	1.529443937008934E-5	4.894220598428588E-4	0.0	0.041119100246485185	6.270720141736629E-4
48-49	1.529443937008934E-5	4.894220598428588E-4	0.0	0.04839925338664771	6.270720141736629E-4
50-51	1.529443937008934E-5	5.123637188979928E-4	0.0	0.05592411755673166	6.270720141736629E-4
52-53	1.529443937008934E-5	5.353053779531268E-4	0.0	0.06311250406067365	6.347192338587075E-4
54-55	2.294165905513401E-5	6.882497716540201E-4	0.0	0.07083619594256876	6.729553322839309E-4
56-57	3.058887874017868E-5	7.494275291343776E-4	0.0	0.07909519320241701	6.882497716540201E-4
58-59	3.058887874017868E-5	7.953108472446456E-4	0.0	0.08766007924966704	7.035442110241095E-4
60-61	3.058887874017868E-5	8.564886047250029E-4	0.0	0.09647732354652355	7.111914307091542E-4
62-63	3.058887874017868E-5	8.641358244100476E-4	0.0	0.10505750403314366	7.341330897642882E-4
64-65	3.058887874017868E-5	8.870774834651816E-4	0.0	0.11278884313472382	7.341330897642882E-4
66-67	3.058887874017868E-5	9.48255240945539E-4	0.0	0.12246257603630534	7.341330897642882E-4
68-69	3.058887874017868E-5	9.635496803156283E-4	0.0	0.13212866171820178	7.341330897642882E-4
70-71	3.058887874017868E-5	9.864913393707623E-4	0.0	0.14203181121033465	7.647219685044668E-4
72-73	4.5883318110268014E-5	0.0010094329984258962	0.0	0.15249320773947575	9.329608015754496E-4
74-75	4.5883318110268014E-5	0.0010094329984258962	0.0	0.16225106005759274	9.788441196857177E-4
76-77	4.5883318110268014E-5	0.0010247274377959857	0.0	0.17197067627728452	0.0010247274377959857
78-79	4.5883318110268014E-5	0.0010247274377959857	0.0	0.18298267262374884	0.0010323746574810303
80-81	6.117775748035736E-5	0.0010247274377959857	0.0	0.1941858494623393	0.0010476690968511198
82-83	6.117775748035736E-5	0.0010247274377959857	0.0	0.20553432347494557	0.0011470829527567004
84-85	6.117775748035736E-5	0.001040021877166075	0.0	0.21702044744188265	0.001154730172441745
86	6.117775748035736E-5	0.001040021877166075	0.0	0.2257612195418887	0.0011623773921267896
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7235	0.0	41.673683	1
GTATCAA	12925	0.0	41.455444	1
TCAACGC	16685	0.0	31.435726	4
TATCAAC	17230	0.0	30.680872	2
CAACGCA	17040	0.0	30.59247	5
ATCAACG	17565	0.0	29.88691	3
AACGCAG	17970	0.0	29.154491	6
GTACCTG	6700	0.0	28.443607	1
GTACATG	24810	0.0	27.608023	1
TACCTGG	6755	0.0	26.606295	2
TACATGG	25430	0.0	26.250568	2
ACATGGG	26155	0.0	24.523739	3
ACGCAGA	21545	0.0	23.970388	7
CGCAGAG	22570	0.0	22.876715	8
GAGTACA	18905	0.0	22.255053	1
AGTACAT	17780	0.0	21.480133	2
GCAGAGT	25150	0.0	20.147375	9
CATGGGG	20430	0.0	20.107744	4
ACCTGGG	9365	0.0	18.799536	3
AGAGTAC	21730	0.0	18.721956	10-11
>>END_MODULE