FastQCFastQC Report
Mon 15 Aug 2016
SRR2921424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6538324
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93630.14320183582214646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133150.042.995441
GGTATCA72800.042.6555331
TCAACGC169950.033.1479534
CAACGCA175500.032.0010875
ATCAACG176700.031.9349653
TATCAAC179050.031.7533652
AACGCAG184550.030.5096866
GTACATG254200.027.5048731
GTACCTG64700.027.2368321
TACATGG261350.026.15222
ACATGGG262400.025.1615583
ACGCAGA224650.024.9167337
TACCTGG69200.024.5723612
CGCAGAG235550.023.662948
GAGTACA187950.022.173021
CATGGGG205900.020.9009044
TATACCG5250.020.7981915
AGTACAT187800.020.6378522
GCAGAGT267900.020.5036459
AGAGTAC226850.019.36844310-11
Produced by FastQC (version 0.11.5)