##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921424_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6538324
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58587414756442	33.0	31.0	34.0	30.0	34.0
2	31.703866770750423	33.0	31.0	34.0	30.0	34.0
3	31.828796798690306	34.0	31.0	34.0	30.0	34.0
4	35.415384125962554	37.0	35.0	37.0	33.0	37.0
5	35.17243363895702	37.0	35.0	37.0	32.0	37.0
6	35.20843231384679	37.0	35.0	37.0	32.0	37.0
7	35.120692550568	37.0	35.0	37.0	32.0	37.0
8	35.061108320725616	37.0	35.0	37.0	32.0	37.0
9	36.62151003835233	39.0	37.0	39.0	32.0	39.0
10-11	36.50775642198215	39.0	37.0	39.0	32.0	39.0
12-13	36.3614661035458	39.0	37.0	39.0	32.0	39.0
14-15	37.55655654262468	40.0	38.0	41.0	32.0	41.0
16-17	37.43402491219462	40.0	37.5	41.0	32.0	41.0
18-19	37.30985180605917	40.0	37.0	41.0	32.0	41.0
20-21	37.175830304524524	40.0	37.0	41.0	31.0	41.0
22-23	37.128433831055176	40.0	37.0	41.0	31.5	41.0
24-25	37.172761016431735	40.0	37.0	41.0	31.0	41.0
26-27	37.031540498757785	40.0	37.0	41.0	31.0	41.0
28-29	36.862714512159386	40.0	37.0	41.0	30.5	41.0
30-31	36.68661976677815	40.0	36.5	41.0	30.0	41.0
32-33	36.48566658672773	39.0	36.0	41.0	30.0	41.0
34-35	36.3072724753316	39.0	36.0	40.5	30.0	41.0
36-37	36.16476913655548	39.0	35.5	40.0	30.0	41.0
38-39	36.09237137835323	39.0	35.0	40.0	29.5	41.0
40-41	35.880396642931736	39.0	35.0	40.0	28.5	41.0
42-43	35.58500932349024	39.0	35.0	40.0	27.5	41.0
44-45	35.326941965555704	38.0	35.0	40.0	27.0	41.0
46-47	34.98383691294589	38.0	34.0	40.0	26.0	41.0
48-49	34.56908360613515	38.0	34.0	40.0	24.0	41.0
50-51	34.22668026240363	38.0	33.0	40.0	23.5	41.0
52-53	33.84639710421203	38.0	33.0	40.0	23.0	41.0
54-55	33.45622586155106	37.0	33.0	40.0	21.5	41.0
56-57	33.00874398393228	37.0	32.0	39.5	19.5	41.0
58-59	32.54543220862105	36.0	31.0	39.0	18.0	40.0
60-61	32.05006940616586	35.5	31.0	39.0	14.0	40.0
62-63	31.54486715555852	35.0	30.5	38.5	10.5	40.0
64-65	30.958851993263107	35.0	29.5	38.0	7.5	40.0
66-67	30.359132325042317	34.0	29.0	37.5	6.5	39.5
68-69	29.793833404401497	34.0	28.5	37.0	2.0	39.0
70-71	29.171202436587727	34.0	27.5	36.0	2.0	39.0
72-73	28.55258342963732	33.0	26.0	35.5	2.0	38.5
74-75	27.972183773701026	33.0	26.0	35.0	2.0	37.5
76-77	26.899788692025663	31.5	25.5	34.0	2.0	36.0
78-79	27.815305344305358	33.0	26.0	35.0	2.0	36.0
80-81	27.800269457432822	33.0	26.0	35.0	2.0	36.0
82-83	27.381545867106006	33.0	26.0	35.0	2.0	36.0
84-85	26.788828605006422	32.5	25.0	35.0	2.0	35.0
86-87	26.153187804703467	32.0	24.0	34.0	2.0	35.0
88-89	25.514757757492593	32.0	21.5	34.0	2.0	35.0
90-91	24.904445619397265	31.0	19.5	34.0	2.0	35.0
92-93	24.329064910212466	31.0	17.0	34.0	2.0	35.0
94-95	23.66906909171219	31.0	4.5	34.0	2.0	35.0
96-97	22.71458358441705	30.0	2.0	34.0	2.0	35.0
98-99	21.51363116908859	29.5	2.0	34.0	2.0	35.0
100-101	18.11972586552762	23.5	2.0	31.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62395.0
3	30324.0
4	18398.0
5	13964.0
6	12259.0
7	12678.0
8	15003.0
9	17707.0
10	21199.0
11	24820.0
12	29619.0
13	34868.0
14	39262.0
15	44853.0
16	51013.0
17	59004.0
18	60161.0
19	56221.0
20	57142.0
21	59531.0
22	63376.0
23	68343.0
24	76436.0
25	87519.0
26	102796.0
27	122311.0
28	147039.0
29	176857.0
30	211357.0
31	258291.0
32	322543.0
33	415549.0
34	556791.0
35	774064.0
36	1042657.0
37	1032548.0
38	350789.0
39	8637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.795262029562	17.489783275654112	13.233492384593742	24.481462310190153
2	16.51183086063034	18.71865022290116	38.35435503043287	26.415163886035625
3	20.642425301046522	22.135417821782784	27.527756054159543	29.69440082301115
4	13.956765809803965	14.459194626394414	34.16993421642621	37.414105347375404
5	15.478463714753596	36.1030428287759	32.55031016514866	15.868183291321847
6	36.23669901397091	32.82431603127501	15.845424675060334	15.093560279693738
7	32.2968550717606	30.24187740937269	18.35153948076703	19.109728038099675
8	28.796063612482804	34.105422285313075	17.85387635696454	19.244637745239583
9	27.14167009317553	16.188608723100632	18.323856982469845	38.345864201253995
10-11	27.222777379535863	24.956260790325437	25.35601998260153	22.464941847537176
12-13	27.12375813560727	21.74014448043983	26.50249392831427	24.633603455638635
14-15	24.000277309643458	23.328566627467858	23.939053400777325	28.73210266211136
16-17	24.50634146214588	26.67772223727969	24.49675619368607	24.319180106888368
18-19	24.569928333692758	25.681884093837493	26.182234733261968	23.565952839207785
20-21	25.065407185209637	24.769425473137407	26.206513295808453	23.9586540458445
22-23	24.752701348698654	24.51122650690545	26.1499336602113	24.5861384841846
24-25	24.392831748501514	24.90114068877361	26.193424066723964	24.512603496000914
26-27	24.514964593069262	25.01776427310583	26.172996560959106	24.2942745728658
28-29	24.417480343520747	25.203968982258168	26.13848022427352	24.240070449947563
30-31	24.575011299128267	25.074979562152087	26.05797695513156	24.292032183588084
32-33	24.281591230065402	24.978512072586405	26.11374151332978	24.626155184018412
34-35	24.572465829692987	25.00707420187707	26.200893567294397	24.21956640113554
36-37	24.501615832288426	25.21919756365148	25.990697731333988	24.288488872726106
38-39	24.57099943072972	24.98548907644291	26.088778170675997	24.354733322151375
40-41	24.598200497504294	25.030032756470867	25.98197896934525	24.38978777667959
42-43	24.626788055504743	24.958650117128688	26.06935370293669	24.34520812442988
44-45	24.57895701324797	24.988420572161406	25.97599892068107	24.456623493909557
46-47	24.597559299351264	25.172996359593782	25.894920129137162	24.334524211917795
48-49	24.62089863522744	25.244109236254424	25.721093659545446	24.41389846897269
50-51	24.621951315575235	25.32955055854102	25.53945035559302	24.509047770290728
52-53	24.66340765375542	25.427749268297458	25.62736916228624	24.281473915660882
54-55	24.633688813698328	25.19178296846562	25.680949891133515	24.49357832670254
56-57	24.636729991777443	25.31799699974549	25.616324712699935	24.428948295777133
58-59	24.707312758971227	25.304781454916725	25.623559391002225	24.364346395109823
60-61	24.783702570270854	25.182885009883776	25.574809865053282	24.458602554792087
62-63	24.488765111841797	25.30650077557811	25.774218622547878	24.43051549003221
64-65	24.620569190189183	25.09922551406151	25.75764945391361	24.5225558418357
66-67	24.579385351463305	25.38242163662239	25.62424058636926	24.413952425545045
68-69	24.715931741897116	25.272873713756038	25.589779557002114	24.421414987344733
70-71	24.59109086260419	25.16202370415262	25.71764499112932	24.529240442113874
72-73	24.79976482157987	25.198085648884067	25.481303376796156	24.52084615273991
74-75	24.586314244864692	25.341732256475336	25.45563216976695	24.616321328893022
76-77	24.633975299195495	25.161713561266456	25.690548641468027	24.513762498070022
78-79	24.580357634129633	25.072767090135994	25.766295714893662	24.58057956084071
80-81	24.51487952672651	25.364852974857342	25.52017873209814	24.600088766318002
82-83	24.638720679986797	25.432841780452293	25.430072755741804	24.498364783819106
84-85	24.679466636153887	25.303454058197524	25.551903267952657	24.465176037695933
86-87	24.625912952924065	25.301511706185437	25.54820432016019	24.52437102073031
88-89	24.665473673044417	25.123154299735035	25.56849758903061	24.642874438189942
90-91	24.638116607585538	25.219065715733098	25.489662793468426	24.653154883212935
92-93	24.65102714143236	25.42647477813641	25.59892993795868	24.323568142472553
94-95	24.604185389475337	25.19568274688736	25.580000778547635	24.62013108508967
96-97	24.61455514062862	25.243061105405122	25.551322162171257	24.591061591795
98-99	24.67967786414638	25.204436163214865	25.549619960547144	24.566266012091607
100-101	25.23401216714138	24.562828677205957	25.638479718384676	24.56467943726799
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	268.0
1	234.5
2	464.0
3	737.0
4	545.0
5	394.5
6	889.5
7	2166.0
8	3310.5
9	3294.0
10	2396.5
11	1624.5
12	1438.5
13	1520.0
14	1700.0
15	1875.0
16	2111.0
17	2397.0
18	2737.5
19	3075.0
20	3344.5
21	3714.5
22	4287.5
23	5310.0
24	6398.0
25	7714.0
26	9543.5
27	12219.5
28	15996.5
29	20837.5
30	27097.0
31	34121.0
32	41700.0
33	49981.0
34	58687.0
35	69015.0
36	82576.0
37	96921.5
38	111100.5
39	124938.0
40	139323.5
41	153783.0
42	169840.0
43	191101.0
44	213277.0
45	235239.5
46	250183.5
47	262398.5
48	280172.0
49	288910.0
50	287918.5
51	287510.0
52	288446.5
53	289497.0
54	288916.5
55	280614.5
56	262871.5
57	242595.5
58	225219.0
59	207543.0
60	182499.5
61	154548.5
62	127120.5
63	98437.5
64	73424.0
65	55422.5
66	42318.0
67	32627.5
68	25291.5
69	19602.5
70	15384.5
71	11950.0
72	9236.0
73	7451.5
74	5843.5
75	4398.5
76	3307.5
77	2180.5
78	1355.0
79	838.5
80	538.5
81	315.0
82	162.0
83	75.0
84	35.0
85	19.0
86	12.5
87	9.5
88	6.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	1.0
96	1.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3072652869450948
2	0.0
3	0.0058271814000040376
4	0.05912830260476538
5	5.047164992129481E-4
6	0.06169776841894039
7	0.07226622602367212
8	0.1017080218110941
9	0.09057366994966906
10-11	0.09393079939140367
12-13	0.09420609930006527
14-15	0.06289073468980735
16-17	0.034450724681126234
18-19	0.03682136278349008
20-21	0.027094099344113262
22-23	0.04058379486853206
24-25	0.03275304191104632
26-27	0.020295721044108553
28-29	0.022704595244897624
30-31	0.003196537828348672
32-33	0.0053607009992163125
34-35	0.007402508655123239
36-37	0.01027021603701499
38-39	0.01540914766536501
40-41	0.019806298984265693
42-43	0.003303598903939297
44-45	0.013275573373237544
46-47	0.023759911561433786
48-49	0.002523582496064741
50-51	0.006767789421264532
52-53	0.002263577026773222
54-55	0.004121851410239076
56-57	0.0032424211464589397
58-59	0.0011241412937015663
60-61	0.005138931628350017
62-63	0.0144150091063092
64-65	0.006714258883469219
66-67	0.03735666816144321
68-69	0.05951066358901761
70-71	0.01189142661024446
72-73	0.03074182313387957
74-75	0.048949853203970925
76-77	0.2977444984372142
78-79	0.07089737370004913
80-81	0.031521839541754124
82-83	0.026405849572459242
84-85	0.017940377381114793
86-87	0.11508300904023723
88-89	0.5151014235452388
90-91	0.1272497355591433
92-93	0.04591390698900819
94-95	0.007792516859060517
96-97	0.005490703733862072
98-99	0.009903149492132847
100-101	0.007180739284256944
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	6538324.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.08269585342401
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.89191628190714	28.818840842261075
2	15.836863592868818	13.645895548874625
3	6.3850736842275655	8.252585626178332
4	3.2978009891846094	5.683126280086455
5	1.9448239112391934	4.189412852819235
6	1.24459144737924	3.2172212873527553
7	0.8422119231265676	2.5399332089732467
8	0.5826173068482112	2.0080579383905994
9	0.4416579068202502	1.712503194371705
>10	2.306616967974403	17.70251105296635
>50	0.14775337667545435	4.344833022999691
>100	0.07308301948357068	5.621494760490939
>500	0.003670948688537281	1.0733690900018236
>1k	0.0011760875144952493	0.781746217298936
>5k	1.425560620062254E-4	0.40846907693411943
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9363	0.14320183582214646	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.058887874017868E-5	0.0
3	0.0	0.0	0.0	6.117775748035736E-5	0.0
4	0.0	0.0	0.0	1.0706107559062536E-4	0.0
5	0.0	3.058887874017868E-5	0.0	1.0706107559062536E-4	0.0
6	0.0	3.058887874017868E-5	0.0	1.0706107559062536E-4	0.0
7	0.0	3.058887874017868E-5	0.0	1.0706107559062536E-4	0.0
8	0.0	3.058887874017868E-5	0.0	1.0706107559062536E-4	1.529443937008934E-5
9	0.0	3.058887874017868E-5	0.0	2.2941659055134006E-4	1.529443937008934E-5
10-11	0.0	3.058887874017868E-5	0.0	2.523582496064741E-4	1.529443937008934E-5
12-13	0.0	3.8236098425223346E-5	0.0	2.752999086616081E-4	3.058887874017868E-5
14-15	0.0	8.411941653549136E-5	0.0	3.1353600708683145E-4	4.5883318110268014E-5
16-17	0.0	1.223555149607147E-4	0.0	3.517721055120548E-4	4.5883318110268014E-5
18-19	0.0	1.3764995433080405E-4	0.0	4.0530264330736746E-4	4.5883318110268014E-5
20-21	0.0	1.5294439370089338E-4	0.0	4.2059708267745677E-4	4.5883318110268014E-5
22-23	0.0	1.5294439370089338E-4	0.0	5.276581582680821E-4	4.5883318110268014E-5
24-25	0.0	1.6823883307098272E-4	0.0	6.117775748035735E-4	6.117775748035736E-5
26-27	0.0	1.8353327244107205E-4	0.0	7.035442110241095E-4	6.117775748035736E-5
28-29	0.0	1.8353327244107205E-4	0.0	9.559024606305836E-4	6.117775748035736E-5
30-31	0.0	1.988277118111614E-4	0.0	0.0019118049212611672	6.117775748035736E-5
32-33	0.0	1.988277118111614E-4	0.0	0.003724195986616754	6.117775748035736E-5
34-35	0.0	2.0647493149620607E-4	0.0	0.006018361892130154	9.176663622053603E-5
36-37	3.058887874017868E-5	2.752999086616081E-4	0.0	0.009711969000006728	9.176663622053603E-5
38-39	4.588331811026802E-5	3.517721055120548E-4	0.0	0.015279144930719248	9.176663622053603E-5
40-41	6.117775748035736E-5	3.670665448821441E-4	0.0	0.021427509557495162	9.176663622053603E-5
42-43	6.117775748035736E-5	3.976554236223228E-4	0.0	0.027468813108680448	9.94138559055807E-5
44-45	6.117775748035736E-5	3.976554236223228E-4	0.0	0.033770122129157254	1.1470829527567003E-4
46-47	6.117775748035736E-5	4.1294986299241214E-4	0.0	0.040384967156720894	1.5294439370089338E-4
48-49	6.117775748035736E-5	4.2059708267745677E-4	0.0	0.04742805648664704	1.8353327244107205E-4
50-51	6.117775748035736E-5	4.2824430236250145E-4	0.0	0.054647031869329205	1.911804921261167E-4
52-53	6.117775748035736E-5	4.2824430236250145E-4	0.0	0.06148364626775914	2.1412215118125073E-4
54-55	6.117775748035736E-5	4.894220598428587E-4	0.0	0.06880203550634689	2.3706381023638474E-4
56-57	6.117775748035736E-5	5.047164992129481E-4	0.0	0.07677808563784848	2.447110299214294E-4
58-59	6.117775748035736E-5	5.353053779531268E-4	0.0	0.0848611968449407	2.447110299214294E-4
60-61	6.117775748035736E-5	6.270720141736629E-4	0.0	0.09321196074100947	2.6000546929151873E-4
62-63	6.882497716540202E-5	6.423664535437522E-4	0.0	0.10135624970558205	3.0588878740178677E-4
64-65	7.647219685044669E-5	6.882497716540202E-4	0.0	0.10875111114102023	3.211832267718761E-4
66-67	7.647219685044669E-5	7.647219685044669E-4	0.0	0.11793542198275889	3.4412488582701007E-4
68-69	7.647219685044669E-5	7.800164078745562E-4	0.0	0.127027966188277	3.670665448821441E-4
70-71	7.647219685044669E-5	7.876636275596009E-4	0.0	0.13648757693867725	3.9000820393727815E-4
72-73	7.647219685044669E-5	8.029580669296902E-4	0.0	0.14640602087018018	5.123637188979928E-4
74-75	7.647219685044669E-5	8.182525062997797E-4	0.0	0.15558268449223378	5.429525976381716E-4
76-77	7.647219685044669E-5	8.411941653549135E-4	0.0	0.1649658230457836	5.582470370082608E-4
78-79	7.647219685044669E-5	8.411941653549135E-4	0.0	0.1753889834764995	5.658942566933056E-4
80-81	7.647219685044669E-5	8.794302637801369E-4	0.0	0.18603391327808166	5.735414763783502E-4
82-83	7.647219685044669E-5	8.870774834651816E-4	0.0	0.1967247263977741	6.423664535437522E-4
84-85	7.647219685044669E-5	8.870774834651816E-4	0.0	0.20762201444896278	6.423664535437522E-4
86-87	7.647219685044669E-5	9.023719228352709E-4	0.0	0.2188787218253485	6.576608929138415E-4
88-89	7.647219685044669E-5	9.023719228352709E-4	0.0	0.2314889870859872	6.729553322839309E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	13315	0.0	42.99544	1
GGTATCA	7280	0.0	42.655533	1
TCAACGC	16995	0.0	33.147953	4
CAACGCA	17550	0.0	32.001087	5
ATCAACG	17670	0.0	31.934965	3
TATCAAC	17905	0.0	31.753365	2
AACGCAG	18455	0.0	30.509686	6
GTACATG	25420	0.0	27.504873	1
GTACCTG	6470	0.0	27.236832	1
TACATGG	26135	0.0	26.1522	2
ACATGGG	26240	0.0	25.161558	3
ACGCAGA	22465	0.0	24.916733	7
TACCTGG	6920	0.0	24.572361	2
CGCAGAG	23555	0.0	23.66294	8
GAGTACA	18795	0.0	22.17302	1
CATGGGG	20590	0.0	20.900904	4
TATACCG	525	0.0	20.798191	5
AGTACAT	18780	0.0	20.637852	2
GCAGAGT	26790	0.0	20.503645	9
AGAGTAC	22685	0.0	19.368443	10-11
>>END_MODULE