FastQCFastQC Report
Mon 15 Aug 2016
SRR2921423_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921423_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4454209
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT67220.15091343940080046No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54740.12289499662005082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG49750.029.4218541
TACCTGG53000.026.6427972
GTACATG127200.025.2221241
TACATGG131950.023.7657622
ACATGGG135600.022.2129273
CGTATAC3001.0913936E-1121.3947353
GTATAGG11000.021.3409391
ACCTGGG66500.020.4755213
GTATCAA153150.020.1970651
GAGTACA101650.019.6978071
AGTACAT93500.019.6134242
CATGGGG108150.017.7618274
TCAACGC171550.017.6381634
CAACGCA173450.017.4978165
AGAGTAC125350.017.45166810-11
TATCAAC174500.017.3139042
GTGTAGC25100.017.238131
ATCAACG176050.017.1612743
AACGCAG181150.016.7990026
GTATATA12050.016.4254551
Produced by FastQC (version 0.11.5)