##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921423_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4454209
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.079369872406076	31.0	31.0	34.0	26.0	34.0
2	30.17026771756781	31.0	31.0	34.0	26.0	34.0
3	30.302545973931622	31.0	31.0	34.0	26.0	34.0
4	33.78903257570536	37.0	35.0	37.0	30.0	37.0
5	33.76781960612984	37.0	35.0	37.0	29.0	37.0
6	33.868102058075856	37.0	35.0	37.0	30.0	37.0
7	33.74513140268003	37.0	35.0	37.0	29.0	37.0
8	33.71755164609474	37.0	35.0	37.0	29.0	37.0
9	35.14314528123849	39.0	35.0	39.0	29.0	39.0
10-11	35.00532058554055	39.0	35.0	39.0	28.0	39.0
12-13	34.83170973791306	39.0	35.0	39.0	27.0	39.0
14-15	35.80532783710868	39.5	36.0	41.0	27.0	41.0
16-17	35.653877265301205	39.0	36.0	41.0	27.0	41.0
18-19	35.46946101990275	39.0	36.0	41.0	27.0	41.0
20-21	35.34677941695147	39.0	36.0	40.0	26.0	41.0
22-23	35.37631597439635	39.0	36.0	41.0	26.5	41.0
24-25	35.233337389422005	39.0	35.5	41.0	25.0	41.0
26-27	34.947431407013006	39.0	35.0	40.0	24.0	41.0
28-29	34.70373145939043	39.0	34.5	40.0	23.5	41.0
30-31	34.43563032179227	38.5	34.0	40.0	22.0	41.0
32-33	34.15060126276069	38.0	34.0	40.0	19.5	41.0
34-35	33.71342588549392	38.0	33.0	40.0	16.5	41.0
36-37	33.31158551383646	38.0	33.0	40.0	14.0	41.0
38-39	33.064600807910004	38.0	32.5	40.0	9.0	41.0
40-41	32.7870632024676	38.0	32.0	40.0	9.0	41.0
42-43	32.47761735024109	38.0	31.0	40.0	8.0	41.0
44-45	32.14753012263232	37.0	31.0	40.0	7.5	41.0
46-47	31.832168741969674	37.0	30.5	40.0	3.5	41.0
48-49	31.592294613925837	37.0	30.0	40.0	2.0	41.0
50-51	30.858239364160955	36.0	29.5	39.0	2.0	40.0
52-53	31.390238087166544	36.5	30.5	39.0	2.0	40.0
54-55	31.81124482483871	37.0	31.0	40.0	2.0	40.5
56-57	31.6458948154431	37.0	31.0	40.0	2.0	41.0
58-59	31.30129064442194	36.0	30.5	39.0	2.0	41.0
60-61	30.83145582077536	35.5	30.0	39.0	2.0	40.0
62-63	30.34327284597557	35.0	29.0	39.0	2.0	40.0
64-65	29.790197092233434	35.0	28.5	38.0	2.0	40.0
66-67	29.287580578280004	34.5	28.0	37.5	2.0	40.0
68-69	28.755038212171904	34.0	26.5	37.0	2.0	39.0
70-71	28.22121728010518	34.0	26.0	36.0	2.0	39.0
72-73	27.655198487542904	33.5	26.0	36.0	2.0	39.0
74-75	27.05245690087735	33.0	24.5	35.0	2.0	37.5
76-77	26.45470362975783	33.0	23.5	35.0	2.0	37.0
78-79	25.874335039060806	32.0	21.0	35.0	2.0	36.5
80-81	25.274221752953217	32.0	19.0	35.0	2.0	36.0
82-83	24.629445430153815	31.0	16.0	34.0	2.0	35.5
84-85	23.990895128630022	31.0	7.5	34.0	2.0	35.0
86-87	23.357624776924474	30.5	3.5	34.0	2.0	35.0
88-89	22.681202992495415	30.0	2.0	34.0	2.0	35.0
90-91	21.919912716264548	29.0	2.0	34.0	2.0	35.0
92-93	21.085377111850836	29.0	2.0	34.0	2.0	35.0
94-95	20.16736686581164	28.0	2.0	33.5	2.0	34.5
96-97	19.27396637652162	26.5	2.0	33.0	2.0	34.0
98	18.65687869608274	25.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	168609.0
3	28249.0
4	21185.0
5	18066.0
6	16361.0
7	17705.0
8	19926.0
9	22818.0
10	25451.0
11	27779.0
12	30177.0
13	33819.0
14	29777.0
15	29771.0
16	33395.0
17	37849.0
18	42147.0
19	40709.0
20	42608.0
21	46277.0
22	50023.0
23	55366.0
24	61388.0
25	68751.0
26	77351.0
27	87867.0
28	100786.0
29	116722.0
30	137880.0
31	164835.0
32	204392.0
33	258387.0
34	344218.0
35	470250.0
36	618306.0
37	629470.0
38	263894.0
39	11645.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.70093725673923	16.68369327562106	12.841348922473927	24.77402054516578
2	16.248809523809523	18.402380952380952	38.7702380952381	26.57857142857143
3	20.998269163593804	21.67263267967396	27.308487612894677	30.020610543837563
4	13.859460314272692	14.157972873072747	34.478279162367784	37.50428765028678
5	15.59476551530469	35.549205279415894	32.69537770289245	16.16065150238697
6	35.7849269987642	32.75760705204227	16.295772777910948	15.161693171282595
7	31.92801771324329	29.788453599541086	18.73128760167304	19.552241085542583
8	28.362785437959715	33.47929146086571	18.280110263506067	19.877812837668515
9	26.93030606968338	16.628574392426543	18.637484369593633	37.80363516829645
10-11	27.141644624276417	24.669240649011797	25.684096247030453	22.505018479681333
12-13	27.31483835955144	21.618177170357253	26.298592640966888	24.768391829124415
14-15	24.026423982773217	23.35295821516864	23.972904639690313	28.64771316236783
16-17	24.566332516388485	26.500369926699502	24.510175510291145	24.42312204662087
18-19	24.517209144770487	25.83137876336849	25.935090868269338	23.716321223591684
20-21	25.025474591356094	24.890782253641323	26.005013354640237	24.07872980036234
22-23	24.69298011518618	24.66275371495381	25.889619703567785	24.754646466292225
24-25	24.547433620564735	24.87602544530977	25.989993518477956	24.586547415647537
26-27	24.5883864235491	24.94990351455101	26.124019528978444	24.337690532921446
28-29	24.47917205017709	25.297810178957995	25.915900841243257	24.307116929621657
30-31	24.71151449680439	25.065124677830386	25.75371151415985	24.469649311205377
32-33	24.461639298020877	25.132493860259725	25.81992243675739	24.58594440496201
34-35	24.802155095078255	25.039997241103233	25.908305902145717	24.249541761672795
36-37	24.763324112877	24.981291950506606	25.876341515401148	24.37904242121525
38-39	24.65116872434209	25.13831830545554	25.848043877105535	24.362469093096824
40-41	24.818729632519197	25.10715240844702	25.784357210292818	24.289760748740967
42-43	24.67017324672408	25.00249139860854	25.720849366597875	24.6064859880695
44-45	24.722148529216113	24.996583666211947	25.69779252365113	24.583475280920812
46-47	24.917561924886883	25.113833653419672	25.61817132067799	24.350433101015454
48-49	24.819024934643192	25.16186330420012	25.491388876729363	24.527722884427323
50-51	24.502919172841978	25.410580966284463	25.595936199074703	24.49056366179886
52-53	24.73248913813242	25.39014694534572	25.418040598478093	24.459323318043772
54-55	24.581465997679096	25.14993909861892	25.61857886692159	24.650016036780393
56-57	24.648774842149546	25.273304269951808	25.59199562334002	24.485925264558624
58-59	24.905019301882984	25.152084757926286	25.51027833589417	24.432617604296563
60-61	24.7954800821713	25.132993518054015	25.675698242768206	24.395828157006484
62-63	24.51575619133084	25.295141525967512	25.650561732071736	24.538540550629918
64-65	24.589828285513214	25.272972815870396	25.55137749138944	24.58582140722694
66-67	24.66318932863667	25.256481904858674	25.47094785686464	24.609380909640016
68-69	24.572637009895058	25.222674052177425	25.534359608621344	24.670329329306174
70-71	24.650302346258396	25.0622169081335	25.615173380039767	24.672307365568336
72-73	24.804501869952695	25.047228090022966	25.53953694819931	24.60873309182503
74-75	24.489410276973608	25.421344688327878	25.472283866745904	24.616961167952606
76-77	24.713356231231103	25.086030624726053	25.406844764183568	24.793768379859277
78-79	24.623843346267762	25.077794311805757	25.581381188575982	24.7169811533505
80-81	24.697377950210516	25.16303880266563	25.48251468812201	24.657068559001843
82-83	24.830413303220674	25.12510789830234	25.448458838954497	24.59601995952249
84-85	24.75239376370047	25.19358223755858	25.384571503558234	24.66945249518271
86-87	24.654253319189557	25.212142557814605	25.497727641955155	24.635876481040683
88-89	24.629651975140597	25.07864137121411	25.411859301469303	24.879847352175986
90-91	24.7143567487848	24.990737326825222	25.395149132984475	24.8997567914055
92-93	24.85704769842172	25.168985653131582	25.458858961245596	24.515107687201105
94-95	24.82748682323738	24.97538183942566	25.43119978196874	24.765931555368226
96-97	24.676010563581173	24.978492409822028	25.539211916794773	24.806285109802026
98	24.739599775588584	25.09524857533026	25.307348030717662	24.85780361836349
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	647.0
1	477.5
2	571.0
3	849.5
4	659.5
5	532.0
6	870.5
7	1723.0
8	2605.5
9	2597.0
10	1979.5
11	1636.5
12	1810.0
13	2158.0
14	2528.5
15	2885.5
16	3146.0
17	3322.5
18	3509.5
19	3544.5
20	3432.5
21	3341.0
22	3300.5
23	3562.0
24	4031.0
25	4788.5
26	6125.0
27	8083.0
28	10472.0
29	13610.0
30	17669.0
31	22774.5
32	29034.0
33	35884.5
34	43699.0
35	52825.5
36	62889.0
37	73882.0
38	84774.0
39	95357.5
40	106450.5
41	118166.5
42	130570.0
43	143878.0
44	156307.0
45	168537.5
46	184732.5
47	200415.0
48	206845.5
49	204124.0
50	201241.0
51	201108.0
52	200939.5
53	200819.0
54	198432.0
55	191647.5
56	181281.0
57	169904.0
58	156547.5
59	140052.5
60	122190.5
61	102746.5
62	83215.0
63	64168.0
64	47452.5
65	35932.5
66	27760.5
67	21442.5
68	16806.0
69	13192.5
70	10086.0
71	7712.0
72	6090.0
73	4811.5
74	3853.5
75	3021.0
76	2117.0
77	1328.5
78	808.5
79	520.5
80	340.0
81	207.5
82	114.5
83	71.0
84	45.0
85	27.5
86	25.5
87	18.5
88	11.5
89	10.5
90	10.5
91	10.0
92	9.5
93	8.5
94	6.0
95	6.0
96	5.5
97	3.0
98	1.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4703865489922004
2	0.049593541748939034
3	0.05846155849444873
4	0.05545316800356696
5	0.06643154822775492
6	0.06389462191827999
7	0.04371146481900602
8	0.019689242242562036
9	0.012347871417798311
10-11	0.015019501779103765
12-13	0.011505971093857517
14-15	0.015244008532154643
16-17	0.014009221390374813
18-19	0.05528478793877881
20-21	0.08297769592760465
22-23	0.10005143449712396
24-25	0.06946238939394177
26-27	0.08524521413341853
28-29	0.0873555776120968
30-31	0.05737270074215198
32-33	0.03267695790655535
34-35	0.39632177116071565
36-37	0.6205029894196703
38-39	0.5698991672820022
40-41	0.5639721890014591
42-43	0.5380641096993877
44-45	0.5228650025178433
46-47	0.5130540574095199
48-49	0.5025134653537812
50-51	0.5160961239133592
52-53	0.5989503411267859
54-55	0.5686194787896123
56-57	0.5260866744241233
58-59	0.502614493392654
60-61	0.5158716171603084
62-63	0.5284439953311576
64-65	0.5461463528092193
66-67	0.573255543240113
68-69	0.5267265186703184
70-71	0.4743715438588535
72-73	0.4581509309509275
74-75	0.4379789991893061
76-77	0.4529985009684098
78-79	0.44720622673969723
80-81	0.44359166801557803
82-83	0.46329213559579263
84-85	0.42977327736529647
86-87	0.4328153438691359
88-89	0.4596551261963684
90-91	0.4432661332236543
92-93	0.43808002722817896
94-95	0.4486206192839177
96-97	0.4429069224187729
98	0.4377208164232976
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4454209.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.256120802720865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.70976867408987	43.34847661149741
2	13.469020033695726	15.423676762870997
3	4.684961752161452	8.047282081136487
4	2.0407945144877626	4.673919090201656
5	1.1218684912747707	3.2116918930597227
6	0.6669804450619866	2.2913227761312984
7	0.45205268715375363	1.8117948285408931
8	0.3140120456307162	1.4383289294513428
9	0.21761074184826673	1.121359223091066
>10	1.225741719677298	12.468710939035063
>50	0.07020906200181763	2.737616832434257
>100	0.0258001996639515	2.392372050300767
>500	5.898168753516486E-4	0.2288522340477166
>1k	5.111741936220477E-4	0.5300182747822237
>5k	7.864218363398501E-5	0.27457747341924266
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	6722	0.15091343940080046	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5474	0.12289499662005082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.490135061017568E-5
2	0.0	0.0	0.0	0.0	4.490135061017568E-5
3	0.0	0.0	0.0	0.0	4.490135061017568E-5
4	0.0	0.0	0.0	0.0	4.490135061017568E-5
5	0.0	0.0	0.0	0.0	4.490135061017568E-5
6	0.0	0.0	0.0	0.0	6.735202591526352E-5
7	0.0	0.0	0.0	0.0	6.735202591526352E-5
8	0.0	0.0	0.0	0.0	6.735202591526352E-5
9	0.0	0.0	0.0	4.490135061017568E-5	6.735202591526352E-5
10-11	1.122533765254392E-5	0.0	0.0	6.735202591526352E-5	6.735202591526352E-5
12-13	2.245067530508784E-5	3.367601295763176E-5	0.0	7.857736356780744E-5	1.5715472713561488E-4
14-15	2.245067530508784E-5	6.735202591526352E-5	0.0	8.980270122035136E-5	2.245067530508784E-4
16-17	2.245067530508784E-5	6.735202591526352E-5	0.0	1.2347871417798314E-4	2.918587789661419E-4
18-19	2.245067530508784E-5	6.735202591526352E-5	0.0	2.132814153983345E-4	3.367601295763176E-4
20-21	6.735202591526352E-5	6.735202591526352E-5	0.0	2.4695742835596627E-4	4.714641814068446E-4
22-23	6.735202591526352E-5	6.735202591526352E-5	0.0	3.2553479192377367E-4	5.163655320170202E-4
24-25	7.857736356780744E-5	6.735202591526352E-5	0.0	3.928868178390372E-4	5.388162073221082E-4
26-27	8.980270122035136E-5	6.735202591526352E-5	0.0	4.490135061017568E-4	5.50041544974652E-4
28-29	8.980270122035136E-5	6.735202591526352E-5	0.0	7.633229603729865E-4	5.61266882627196E-4
30-31	8.980270122035136E-5	6.735202591526352E-5	0.0	0.0020205607774579056	5.61266882627196E-4
32-33	8.980270122035136E-5	6.735202591526352E-5	0.0	0.00524223268373801	5.61266882627196E-4
34-35	8.980270122035136E-5	6.735202591526352E-5	0.0	0.009406832952831803	6.061682332373716E-4
36-37	8.980270122035136E-5	6.735202591526352E-5	0.0	0.01517665650623938	6.061682332373716E-4
38-39	8.980270122035136E-5	6.735202591526352E-5	0.0	0.02506617897813057	6.286189085424594E-4
40-41	8.980270122035136E-5	8.980270122035136E-5	0.0	0.03557309502091168	6.510695838475473E-4
42-43	8.980270122035136E-5	8.980270122035136E-5	0.0	0.04540649080454015	6.735202591526352E-4
44-45	8.980270122035136E-5	8.980270122035136E-5	0.0	0.055408266652956784	7.408722850678987E-4
46-47	8.980270122035136E-5	8.980270122035136E-5	0.0	0.06621826681235658	8.082243109831621E-4
48-49	8.980270122035136E-5	8.980270122035136E-5	0.0	0.07836408215240911	9.541537004662331E-4
50-51	8.980270122035136E-5	1.0102803887289528E-4	0.0	0.09076808025847014	0.0010102803887289528
52-53	8.980270122035136E-5	1.122533765254392E-4	0.0	0.10276796620903958	0.0010664070769916723
54-55	8.980270122035136E-5	1.122533765254392E-4	0.0	0.11601386463904141	0.0011337591029069358
56-57	8.980270122035136E-5	1.122533765254392E-4	0.0	0.12937201644556867	0.0011674351158645676
58-59	8.980270122035136E-5	1.122533765254392E-4	0.0	0.14357206857603672	0.0011674351158645676
60-61	1.122533765254392E-4	1.122533765254392E-4	0.0	0.15718840314857252	0.0011674351158645676
62-63	1.122533765254392E-4	1.122533765254392E-4	0.0	0.17145580730495585	0.001257237817084919
64-65	1.122533765254392E-4	1.122533765254392E-4	0.0	0.18654266110997486	0.001257237817084919
66-67	1.122533765254392E-4	1.122533765254392E-4	0.0	0.20233671118710414	0.001268463154737463
68-69	1.122533765254392E-4	1.122533765254392E-4	0.0	0.21843384538085214	0.0012796884923900068
70-71	1.3470405183052704E-4	1.122533765254392E-4	0.0	0.23434014883450688	0.0013470405183052705
72-73	1.3470405183052704E-4	1.122533765254392E-4	0.0	0.24999949485980566	0.001526645920745973
74-75	1.3470405183052704E-4	1.122533765254392E-4	0.0	0.2656588408851044	0.0016501246349239563
76-77	1.3470405183052704E-4	1.122533765254392E-4	0.0	0.2831815929607254	0.001683800647881588
78-79	1.3470405183052704E-4	1.2347871417798314E-4	0.0	0.3018044281262958	0.001695025985534132
80-81	1.3470405183052704E-4	1.3470405183052704E-4	0.0	0.3209211781485781	0.0017174766608392196
82-83	1.3470405183052704E-4	1.5715472713561488E-4	0.0	0.33940930926231794	0.0017960540244070272
84-85	1.3470405183052704E-4	1.5715472713561488E-4	0.0	0.35779641233718484	0.001818504699712115
86	1.3470405183052704E-4	1.5715472713561488E-4	0.0	0.37203014048061056	0.001818504699712115
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	4975	0.0	29.421854	1
TACCTGG	5300	0.0	26.642797	2
GTACATG	12720	0.0	25.222124	1
TACATGG	13195	0.0	23.765762	2
ACATGGG	13560	0.0	22.212927	3
CGTATAC	300	1.0913936E-11	21.394735	3
GTATAGG	1100	0.0	21.340939	1
ACCTGGG	6650	0.0	20.475521	3
GTATCAA	15315	0.0	20.197065	1
GAGTACA	10165	0.0	19.697807	1
AGTACAT	9350	0.0	19.613424	2
CATGGGG	10815	0.0	17.761827	4
TCAACGC	17155	0.0	17.638163	4
CAACGCA	17345	0.0	17.497816	5
AGAGTAC	12535	0.0	17.451668	10-11
TATCAAC	17450	0.0	17.313904	2
GTGTAGC	2510	0.0	17.23813	1
ATCAACG	17605	0.0	17.161274	3
AACGCAG	18115	0.0	16.799002	6
GTATATA	1205	0.0	16.425455	1
>>END_MODULE