FastQCFastQC Report
Mon 15 Aug 2016
SRR2921423_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921423_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4454209
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT69150.15524641973468242No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56430.12668916074661069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG51450.029.8057691
TACCTGG54100.028.0071682
GTACATG131300.026.3330821
TACATGG135600.024.6597562
ACATGGG139850.023.1636753
TATAACG3500.021.7133962
ACCTGGG68700.021.5025123
GTATCAA166450.020.1427461
GAGTACA104250.019.5066031
CATGGGG109200.019.4874764
AGTACAT99600.018.7417012
AGAGTAC134300.018.20731210-11
GTATAGG10400.017.8591631
TCAACGC186000.017.7489244
CAACGCA187250.017.6470765
TATCAAC191800.017.2855242
GTGTAGC27650.017.0517861
ATCAACG194350.017.0102313
AACGCAG195850.016.9700936
TACACGG10750.016.7900092
Produced by FastQC (version 0.11.5)