##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921423_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4454209
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.773791261254242	33.0	31.0	34.0	30.0	34.0
2	31.887928698451287	34.0	31.0	34.0	30.0	34.0
3	32.01519820915453	34.0	31.0	34.0	30.0	34.0
4	35.569572734463065	37.0	35.0	37.0	33.0	37.0
5	35.3481612560165	37.0	35.0	37.0	33.0	37.0
6	35.38606742521512	37.0	35.0	37.0	33.0	37.0
7	35.31208234728096	37.0	35.0	37.0	33.0	37.0
8	35.258977070900805	37.0	35.0	37.0	33.0	37.0
9	36.85361418828798	39.0	37.0	39.0	33.0	39.0
10-11	36.751123824679084	39.0	37.0	39.0	33.0	39.0
12-13	36.62400765208817	39.0	37.0	39.0	32.0	39.0
14-15	37.872677281196275	40.0	38.0	41.0	33.0	41.0
16-17	37.770054795363215	40.0	38.0	41.0	32.5	41.0
18-19	37.65822528758754	40.0	38.0	41.0	32.0	41.0
20-21	37.53408315595429	40.0	38.0	41.0	32.0	41.0
22-23	37.493007400416104	40.0	38.0	41.0	32.0	41.0
24-25	37.54458816368967	40.0	38.0	41.0	32.0	41.0
26-27	37.419434741387306	40.0	37.5	41.0	32.0	41.0
28-29	37.27264448075966	40.0	37.0	41.0	31.5	41.0
30-31	37.11863756280857	40.0	37.0	41.0	31.0	41.0
32-33	36.94001830628064	40.0	37.0	41.0	31.0	41.0
34-35	36.77471600457006	40.0	36.0	41.0	30.0	41.0
36-37	36.65760407740184	40.0	36.0	41.0	30.0	41.0
38-39	36.609805242636796	40.0	36.0	41.0	30.0	41.0
40-41	36.43151275568793	39.0	36.0	41.0	30.0	41.0
42-43	36.17141988622447	39.0	35.0	41.0	30.0	41.0
44-45	35.94276929977916	39.0	35.0	40.0	29.0	41.0
46-47	35.634710899286496	39.0	35.0	40.0	28.0	41.0
48-49	35.2685543044792	38.0	35.0	40.0	27.0	41.0
50-51	34.95932880563081	38.0	34.0	40.0	26.0	41.0
52-53	34.62712279554013	38.0	34.0	40.0	25.5	41.0
54-55	34.27424521390891	38.0	33.5	40.0	24.0	41.0
56-57	33.85859127849636	37.0	33.0	40.0	23.5	41.0
58-59	33.44164070433156	37.0	33.0	39.5	22.5	41.0
60-61	32.975438399949354	36.0	32.0	39.0	21.5	40.0
62-63	32.51139091138292	35.5	31.5	39.0	19.5	40.0
64-65	31.965393967817853	35.0	31.0	38.5	18.0	40.0
66-67	31.406867863631902	35.0	30.5	38.0	14.0	40.0
68-69	30.870924107961706	34.5	30.0	37.0	10.0	39.0
70-71	30.283428999402588	34.0	29.0	36.5	7.5	39.0
72-73	29.705753254955034	34.0	29.0	36.0	7.0	39.0
74-75	29.15681370137773	34.0	28.0	35.5	4.0	37.5
76-77	27.966561851947226	31.5	26.5	34.0	2.0	36.0
78-79	28.892079828315197	33.5	29.0	35.0	2.0	36.5
80-81	28.902876358069413	34.0	29.0	35.0	2.0	36.0
82-83	28.532485790406334	34.0	28.5	35.0	2.0	36.0
84-85	27.993129419836386	33.0	27.0	35.0	2.0	35.5
86-87	27.43155002380894	33.0	26.0	35.0	2.0	35.0
88-89	26.84727681166286	32.5	25.0	34.5	2.0	35.0
90-91	26.30953576718111	32.0	24.5	34.0	2.0	35.0
92-93	25.80433619078045	32.0	23.5	34.0	2.0	35.0
94-95	25.23241466666697	32.0	21.5	34.0	2.0	35.0
96-97	24.37935994920759	31.0	14.0	34.0	2.0	35.0
98-99	23.27798066951955	31.0	2.0	34.0	2.0	35.0
100-101	19.7547345667884	25.5	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35666.0
3	17940.0
4	10635.0
5	7946.0
6	7061.0
7	7223.0
8	8693.0
9	10256.0
10	12250.0
11	14315.0
12	16567.0
13	19891.0
14	21910.0
15	25425.0
16	28615.0
17	33364.0
18	34188.0
19	30523.0
20	30683.0
21	32752.0
22	34755.0
23	38108.0
24	43248.0
25	49878.0
26	59004.0
27	70315.0
28	85388.0
29	102902.0
30	125429.0
31	155340.0
32	196825.0
33	259814.0
34	360397.0
35	522537.0
36	761358.0
37	849542.0
38	324930.0
39	8536.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.96511954973963	17.217252383934703	13.222772541450162	24.594855524875502
2	16.436521052334992	18.741666589960193	38.385670721782475	26.43614163592234
3	21.07208930560046	21.839435822825738	27.540683263282684	29.547791608291124
4	14.195947416742689	14.573772484118386	34.32196698169686	36.90831311744206
5	15.5787921036148	35.65458590675297	32.718137304425724	16.048484685206514
6	35.93507269870917	32.84576227664756	16.093394381932356	15.12577064271091
7	32.04389775703185	29.817735247438264	18.609320207563073	19.529046787966806
8	28.368495785405134	33.62472688175716	18.21663282794753	19.79014450489017
9	26.797771013376348	16.709152660595482	18.654976726334322	37.83809959969385
10-11	27.166946728667956	24.763256314878454	25.632150780662293	22.437646175791297
12-13	27.284156474547324	21.64349960954398	26.35294976881633	24.719394147092366
14-15	23.97237170084147	23.370705515494276	24.05821168643671	28.598711097227543
16-17	24.519811620240436	26.556733181714105	24.640516336348536	24.28293886169692
18-19	24.463302334528585	25.92481252316825	26.00046693042209	23.611418211881077
20-21	24.974850972573158	24.893792448633274	26.10540347865592	24.025953100137652
22-23	24.60481267141929	24.637761559877195	26.096530360761133	24.66089540794239
24-25	24.49572037051531	24.869608943051727	26.164171175297664	24.4704995111353
26-27	24.49485071312155	24.991747577829884	26.269166390837405	24.244235318211153
28-29	24.411150531547115	25.347783234859595	26.0501314467814	24.19093478681189
30-31	24.62442943864767	25.065039026783786	25.915611982021332	24.39491955254721
32-33	24.38674303193495	25.116803767985886	25.96001140112994	24.536441798949223
34-35	24.703816316046616	25.06267647756175	26.089400928280952	24.144106278110673
36-37	24.614348837890727	25.051667325851447	26.03430357923302	24.299680257024807
38-39	24.520812811227767	25.20333865420494	26.03894527735408	24.23690325721321
40-41	24.687334533635788	25.133304979967765	25.919875095490514	24.259485390905937
42-43	24.551535917707504	25.102179167405225	25.84566879210411	24.50061612278316
44-45	24.597511273456572	24.995155607718065	25.866641009537673	24.540692109287694
46-47	24.697428207128514	25.247139221199877	25.78014299065966	24.275289581011947
48-49	24.675820498503512	25.271463919349653	25.661577188252327	24.39113839389451
50-51	24.41042142913354	25.445979073753776	25.705325951156528	24.43827354595616
52-53	24.654197623648784	25.469817398474103	25.496882365058244	24.37910261281887
54-55	24.58238409998309	25.165181757746296	25.653357305614932	24.59907683665568
56-57	24.632279809055365	25.357785448172525	25.578460220391886	24.431474522380224
58-59	24.863602151473092	25.252890136911414	25.499614685830036	24.38389302578546
60-61	24.762732164260527	25.239720695094313	25.717899171427476	24.27964796921768
62-63	24.50360531556419	25.36425329561971	25.674274732388426	24.457866656427672
64-65	24.563533506371858	25.358192774614462	25.57395769099208	24.5043160280216
66-67	24.662640027633824	25.344670449099095	25.469195604999783	24.5234939182673
68-69	24.56617675964978	25.292467188210782	25.583861710312757	24.557494341826676
70-71	24.646817390695176	25.163457504416854	25.639894913880777	24.549830191007192
72-73	24.812063001844674	25.13836169512278	25.552851652278036	24.49672365075451
74-75	24.49893636603878	25.521801716526976	25.431223522163133	24.54803839527111
76-77	24.70395642366457	25.230308656326894	25.452187232777728	24.613547687230806
78-79	24.57326662013773	25.211865620563138	25.63698419240558	24.57788356689355
80-81	24.63475495220497	25.29508637780938	25.553128349043426	24.51703032094223
82-83	24.756204723132775	25.262265891971268	25.5155434998144	24.465985885081558
84-85	24.625573090955225	25.380472888805055	25.477646435370048	24.51630758486967
86-87	24.56503816704674	25.371140309355138	25.531756124686062	24.532065398912057
88-89	24.540080052011014	25.28689215454637	25.46379008383188	24.709237709610733
90-91	24.613282247535327	25.197180420028126	25.47706354373105	24.712473788705495
92-93	24.81029575186245	25.38655957180022	25.472630781575774	24.33051389476155
94-95	24.711209682797104	25.22400348283963	25.453670621533746	24.611116212829522
96-97	24.53472931098413	25.215789302930585	25.666547558627595	24.582933827457694
98-99	24.59407350460128	25.286409556684887	25.47249942857297	24.647017510140863
100-101	25.14485470410551	24.60602989384825	25.59730498907745	24.651810412968796
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	295.0
1	246.5
2	452.0
3	663.0
4	458.5
5	346.0
6	679.5
7	1500.0
8	2216.5
9	2208.0
10	1606.5
11	1091.0
12	1017.0
13	1098.5
14	1220.0
15	1287.5
16	1365.5
17	1537.5
18	1782.5
19	1959.0
20	2076.0
21	2249.5
22	2508.0
23	2902.0
24	3439.0
25	4254.0
26	5320.5
27	6817.5
28	8818.5
29	11488.0
30	15368.5
31	19888.0
32	24902.0
33	31010.0
34	37763.0
35	45673.5
36	55487.0
37	66219.0
38	76370.5
39	86377.5
40	97628.5
41	108887.5
42	120456.5
43	132829.0
44	145437.5
45	158111.5
46	169382.5
47	183433.5
48	202539.5
49	208962.0
50	201771.0
51	198219.0
52	198206.0
53	198387.5
54	196270.0
55	189979.5
56	179834.5
57	167394.0
58	154887.0
59	139279.5
60	120547.0
61	101726.0
62	82682.0
63	63637.5
64	47166.0
65	35432.5
66	27204.5
67	21411.0
68	16841.5
69	13023.0
70	9997.5
71	7795.5
72	6130.5
73	4838.0
74	3748.0
75	2886.0
76	2137.5
77	1362.5
78	855.0
79	474.5
80	260.5
81	156.5
82	88.5
83	39.0
84	18.5
85	14.0
86	9.5
87	7.0
88	5.5
89	2.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30883148949678835
2	0.0
3	0.006488245163170385
4	0.060122908467025234
5	4.2656283079666894E-4
6	0.06492735298231403
7	0.07455869268819672
8	0.10486710435006528
9	0.09238452888043647
10-11	0.09657157982483534
12-13	0.0961786930069963
14-15	0.06518553574832255
16-17	0.03608946055292871
18-19	0.03873864023892906
20-21	0.02814192149492761
22-23	0.04172458005450575
24-25	0.03469751868401326
26-27	0.021799605721240293
28-29	0.024156926628274514
30-31	0.0034237279840258956
32-33	0.005938203618195734
34-35	0.008643509992458819
36-37	0.010675296107569267
38-39	0.015221557856849555
40-41	0.019913748995612914
42-43	0.0032665732568902804
44-45	0.013605109234883232
46-47	0.02359565974564732
48-49	0.002649179686000365
50-51	0.007375046837721355
52-53	0.002503250296517294
54-55	0.0039513188536954595
56-57	0.003210446568627561
58-59	0.0011562097782120237
60-61	0.005017725930687132
62-63	0.014503136247086745
64-65	0.006454569150212755
66-67	0.03805389464212389
68-69	0.06025761251885576
70-71	0.011865181898738923
72-73	0.031139086648156834
74-75	0.05061504747532053
76-77	0.2978418839349478
78-79	0.07219014644350995
80-81	0.03244122581585193
82-83	0.026716303613054528
84-85	0.018779989892705976
86-87	0.11411678257576148
88-89	0.5127734239682062
90-91	0.12897912962772962
92-93	0.046731080647540335
94-95	0.008351651213492677
96-97	0.006050456994721173
98-99	0.010035451861374264
100-101	0.007846511019128201
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4454209.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.71193874429916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.5630544134229	37.72227340595296
2	15.535463501404308	16.378088009006387
3	5.443947223208009	8.608830376708141
4	2.5213674125479577	5.316246584084
5	1.346271471899762	3.548228967998886
6	0.8036256325629111	2.54163990702029
7	0.5508178531978789	2.0324273855923067
8	0.3686983256719875	1.5547842846357975
9	0.2766381406583988	1.31239194522501
>10	1.4678135145874602	13.828982816226528
>50	0.08711029388303791	3.1429187795310765
>100	0.033915223803275066	2.932952254332586
>500	6.384968253712778E-4	0.24266262931845636
>1k	5.5336348318421E-4	0.5558014471681796
>5k	8.513284356675087E-5	0.2817712071993743
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	6915	0.15524641973468242	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5643	0.12668916074661069	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.735202591526352E-5
2	0.0	0.0	0.0	0.0	6.735202591526352E-5
3	0.0	0.0	0.0	0.0	6.735202591526352E-5
4	0.0	0.0	0.0	2.245067530508784E-5	8.980270122035136E-5
5	0.0	0.0	0.0	2.245067530508784E-5	1.122533765254392E-4
6	0.0	0.0	0.0	2.245067530508784E-5	1.122533765254392E-4
7	0.0	0.0	0.0	2.245067530508784E-5	1.122533765254392E-4
8	0.0	0.0	0.0	2.245067530508784E-5	1.122533765254392E-4
9	0.0	0.0	0.0	4.490135061017568E-5	1.122533765254392E-4
10-11	0.0	0.0	0.0	6.735202591526352E-5	1.122533765254392E-4
12-13	4.490135061017568E-5	0.0	0.0	7.857736356780744E-5	2.1328141539833447E-4
14-15	4.490135061017568E-5	0.0	0.0	8.980270122035136E-5	2.469574283559662E-4
16-17	4.490135061017568E-5	0.0	0.0	1.122533765254392E-4	2.80633441313598E-4
18-19	4.490135061017568E-5	0.0	0.0	1.6838006478815882E-4	3.8166148018649326E-4
20-21	4.490135061017568E-5	0.0	0.0	1.9083074009324663E-4	4.8268951905938853E-4
22-23	4.490135061017568E-5	0.0	0.0	2.581827660085101E-4	5.949428955848276E-4
24-25	4.490135061017568E-5	1.122533765254392E-5	0.0	3.479854672288615E-4	6.061682332373716E-4
26-27	4.490135061017568E-5	2.245067530508784E-5	0.0	4.041121554915811E-4	7.071962721102669E-4
28-29	4.490135061017568E-5	2.245067530508784E-5	0.0	7.071962721102669E-4	7.633229603729865E-4
30-31	4.490135061017568E-5	2.245067530508784E-5	0.0	0.0019981101021528174	7.633229603729865E-4
32-33	4.490135061017568E-5	2.245067530508784E-5	0.0	0.005219782008432922	7.857736356780743E-4
34-35	4.490135061017568E-5	2.245067530508784E-5	0.0	0.009384382277526717	7.857736356780743E-4
36-37	4.490135061017568E-5	2.245067530508784E-5	0.0	0.015086853805019028	7.969989733306183E-4
38-39	4.490135061017568E-5	3.367601295763176E-5	0.0	0.024897798913342414	8.980270122035136E-4
40-41	6.735202591526352E-5	4.490135061017568E-5	0.0	0.03523633489133536	9.317030251611454E-4
42-43	6.735202591526352E-5	4.490135061017568E-5	0.0	0.04491257594782822	9.990550510764087E-4
44-45	6.735202591526352E-5	4.490135061017568E-5	0.0	0.05455514099136345	0.0010215057263814969
46-47	6.735202591526352E-5	4.490135061017568E-5	0.0	0.06520798642362763	0.0010664070769916723
48-49	6.735202591526352E-5	4.490135061017568E-5	0.0	0.07684866156931568	0.0011786604535171117
50-51	6.735202591526352E-5	4.490135061017568E-5	0.0	0.0889158995458004	0.0012123364664747432
52-53	6.735202591526352E-5	4.490135061017568E-5	0.0	0.10053412401618333	0.001380716531262902
54-55	6.735202591526352E-5	4.490135061017568E-5	0.0	0.1132524315765156	0.0014592938948307095
56-57	6.735202591526352E-5	4.490135061017568E-5	0.0	0.12617279521459365	0.0014929699077883413
58-59	6.735202591526352E-5	4.490135061017568E-5	0.0	0.14000241120252777	0.001526645920745973
60-61	6.735202591526352E-5	4.490135061017568E-5	0.0	0.15342791503497027	0.0015603219337036048
62-63	6.735202591526352E-5	4.490135061017568E-5	0.0	0.16711160163342134	0.0015715472713561487
64-65	6.735202591526352E-5	4.490135061017568E-5	0.0	0.18134532977684703	0.0015715472713561487
66-67	7.857736356780744E-5	4.490135061017568E-5	0.0	0.19647708493247623	0.0016164486219663243
68-69	8.980270122035136E-5	4.490135061017568E-5	0.0	0.21155271339984272	0.0016613499725765001
70-71	8.980270122035136E-5	4.490135061017568E-5	0.0	0.22685284862026006	0.0017174766608392196
72-73	8.980270122035136E-5	4.490135061017568E-5	0.0	0.24160294229570278	0.0018746313879748346
74-75	8.980270122035136E-5	4.490135061017568E-5	0.0	0.2566561200877642	0.001964434089195186
76-77	8.980270122035136E-5	4.490135061017568E-5	0.0	0.2734379998783173	0.001998110102152818
78-79	8.980270122035136E-5	4.490135061017568E-5	0.0	0.2910954560057689	0.0020093354398053615
80-81	8.980270122035136E-5	4.490135061017568E-5	0.0	0.3091233482757545	0.002065462128068081
82-83	8.980270122035136E-5	4.490135061017568E-5	0.0	0.3265899736631128	0.0021440394916358886
84-85	8.980270122035136E-5	4.490135061017568E-5	0.0	0.34415762708934405	0.0021552648292884327
86-87	8.980270122035136E-5	4.490135061017568E-5	0.0	0.3623763500994228	0.0022787435434664157
88-89	1.122533765254392E-4	4.490135061017568E-5	0.0	0.38068487581072197	0.0024022222576443987
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	5145	0.0	29.805769	1
TACCTGG	5410	0.0	28.007168	2
GTACATG	13130	0.0	26.333082	1
TACATGG	13560	0.0	24.659756	2
ACATGGG	13985	0.0	23.163675	3
TATAACG	350	0.0	21.713396	2
ACCTGGG	6870	0.0	21.502512	3
GTATCAA	16645	0.0	20.142746	1
GAGTACA	10425	0.0	19.506603	1
CATGGGG	10920	0.0	19.487476	4
AGTACAT	9960	0.0	18.741701	2
AGAGTAC	13430	0.0	18.207312	10-11
GTATAGG	1040	0.0	17.859163	1
TCAACGC	18600	0.0	17.748924	4
CAACGCA	18725	0.0	17.647076	5
TATCAAC	19180	0.0	17.285524	2
GTGTAGC	2765	0.0	17.051786	1
ATCAACG	19435	0.0	17.010231	3
AACGCAG	19585	0.0	16.970093	6
TACACGG	1075	0.0	16.790009	2
>>END_MODULE