FastQCFastQC Report
Mon 15 Aug 2016
SRR2921422_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921422_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6984007
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT106930.15310694848959916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG277800.024.408951
TACATGG283950.023.3948972
ACATGGG289750.021.9772553
GTATCAA268000.021.5226081
CATGGGG227400.018.9710224
GAGTACA205600.018.762881
TATCAAC308050.018.5286082
TCAACGC310100.018.3172864
CAACGCA311100.018.2436685
ATCAACG314600.018.084553
AGAGTAC223350.017.98717510-11
AGTACAT200150.017.9578842
AACGCAG322150.017.6562886
ACGCAGA356950.015.852897
ATACCGT10450.015.7896296
CGCAGAG371500.015.2281848
GTACCTG65700.015.204531
GTGTAGC35100.015.0823611
ATGGGGG106600.014.8372515
GGTATCA207300.014.6784691
Produced by FastQC (version 0.11.5)