##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921422_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6984007
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.363499349299047	31.0	31.0	34.0	27.0	34.0
2	30.48159158488816	31.0	31.0	34.0	27.0	34.0
3	30.603879978929	31.0	31.0	34.0	27.0	34.0
4	34.12310039208151	37.0	35.0	37.0	30.0	37.0
5	34.10362690071759	37.0	35.0	37.0	30.0	37.0
6	34.198342011971064	37.0	35.0	37.0	30.0	37.0
7	34.082732878131424	37.0	35.0	37.0	30.0	37.0
8	34.0668544576201	37.0	35.0	37.0	30.0	37.0
9	35.514958246748606	39.0	37.0	39.0	30.0	39.0
10-11	35.3826367155703	39.0	35.0	39.0	30.0	39.0
12-13	35.225434123992144	39.0	35.0	39.0	30.0	39.0
14-15	36.218232957097555	40.0	36.0	41.0	29.0	41.0
16-17	36.060193095453656	39.5	36.0	41.0	28.5	41.0
18-19	35.87392516931899	39.0	36.0	41.0	27.0	41.0
20-21	35.75107048145857	39.0	36.0	40.0	27.0	41.0
22-23	35.78804130637326	39.0	36.0	41.0	27.0	41.0
24-25	35.64111712660082	39.0	36.0	41.0	27.0	41.0
26-27	35.36041294059413	39.0	35.5	40.0	26.5	41.0
28-29	35.11932498063075	39.0	35.0	40.0	25.0	41.0
30-31	34.86355841281373	38.5	34.5	40.0	24.5	41.0
32-33	34.5832490574537	38.0	34.0	40.0	24.0	41.0
34-35	34.14417396775232	38.0	33.5	40.0	21.0	41.0
36-37	33.75078661862739	38.0	33.0	40.0	18.0	41.0
38-39	33.50713888173365	38.0	33.0	40.0	16.0	41.0
40-41	33.235291860389026	38.0	32.5	40.0	15.0	41.0
42-43	32.93027927950244	38.0	32.0	40.0	13.0	41.0
44-45	32.60142436856091	37.0	31.5	40.0	9.0	41.0
46-47	32.29411955056746	37.0	31.0	40.0	8.5	41.0
48-49	32.06921112765208	37.0	31.0	40.0	8.0	41.0
50-51	31.331259762483057	36.0	30.5	38.5	7.0	39.5
52-53	31.88309383137789	36.5	31.0	39.0	7.0	40.0
54-55	32.30357186068113	37.0	32.0	39.5	4.0	40.5
56-57	32.128794114897076	37.0	31.5	40.0	2.0	41.0
58-59	31.77691073333689	36.0	31.0	39.0	2.0	41.0
60-61	31.291334544767782	35.5	30.5	39.0	2.0	40.0
62-63	30.78465993805562	35.0	30.0	39.0	2.0	40.0
64-65	30.219947588826873	35.0	29.0	38.0	2.0	40.0
66-67	29.70231995758309	34.5	28.5	37.5	2.0	40.0
68-69	29.16052625663176	34.0	28.0	37.0	2.0	39.0
70-71	28.614312313833594	34.0	27.0	36.0	2.0	39.0
72-73	28.03981024074002	33.5	26.0	35.5	2.0	38.5
74-75	27.42542705068881	33.0	26.0	35.0	2.0	37.5
76-77	26.82439278769337	33.0	25.0	35.0	2.0	37.0
78-79	26.236356005943293	32.0	23.5	35.0	2.0	36.0
80-81	25.632482255530384	32.0	21.0	34.5	2.0	36.0
82-83	24.981134182712015	31.0	19.0	34.0	2.0	35.0
84-85	24.338562518622908	31.0	16.5	34.0	2.0	35.0
86-87	23.694155590050237	30.5	7.5	34.0	2.0	35.0
88-89	23.003539085799886	30.0	2.0	34.0	2.0	35.0
90-91	22.22150894178657	29.5	2.0	34.0	2.0	35.0
92-93	21.35589612095177	29.0	2.0	34.0	2.0	35.0
94-95	20.399553938018677	28.0	2.0	33.0	2.0	34.5
96-97	19.467172641722726	27.0	2.0	33.0	2.0	34.0
98	18.822506048461864	25.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	216253.0
3	37808.0
4	28959.0
5	25281.0
6	23726.0
7	25033.0
8	28280.0
9	32899.0
10	36608.0
11	40286.0
12	44709.0
13	49841.0
14	42180.0
15	42413.0
16	46926.0
17	53382.0
18	59628.0
19	60081.0
20	64212.0
21	70119.0
22	77066.0
23	85427.0
24	95731.0
25	107671.0
26	122556.0
27	140767.0
28	162411.0
29	189792.0
30	225547.0
31	272614.0
32	337675.0
33	433300.0
34	574865.0
35	786428.0
36	1010025.0
37	965519.0
38	355949.0
39	12040.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.697349148544326	17.754160240004797	14.405452792107337	24.143037819343533
2	16.005892289655634	19.15131661129454	37.8595368223695	26.98325427668033
3	19.897762338769954	22.75537961186594	27.667492214553818	29.67936583481029
4	14.237872600432796	15.377143663509388	34.366264980842146	36.01871875521567
5	16.27008825334711	35.748322361125204	31.93167601123985	16.04991337428784
6	35.15021739021418	32.73404461854447	16.640463307297132	15.475274683944216
7	31.265811814606497	30.246578717607314	19.3050501114822	19.18255935630399
8	28.698824523813922	32.089601935778525	18.89255847771688	20.319015062690678
9	27.410443071871804	16.070556063762574	18.91468556623968	37.60431529812595
10-11	26.88600543063821	24.606303074635974	25.656951559171144	22.85073993555467
12-13	27.191667463218117	21.855603300909216	26.126901923215307	24.825827312657363
14-15	24.53518065774128	23.233806792795228	23.870480978752752	28.36053157071074
16-17	24.779632016650773	26.184347683130653	24.404484103417754	24.63153619680082
18-19	24.828432157341922	25.695295224910836	25.57397193067359	23.902300687073648
20-21	25.72163054453614	24.615539275382964	25.268640999838638	24.39418918024226
22-23	25.32235426487003	24.35537925980869	25.222417509177593	25.09984896614369
24-25	25.18376957330526	24.643509989357437	25.219669862062222	24.953050575275082
26-27	25.1313084991088	24.650543230001702	25.383474701514597	24.834673569374903
28-29	24.95419770312086	25.030396330313092	25.17688915844155	24.838516808124492
30-31	25.22021238240983	24.707648430112165	25.04473732789802	25.027401859579985
32-33	25.112512398529006	24.713123999613273	24.988895931134003	25.185467670723718
34-35	25.352534237757716	24.686169611343487	25.098883680215994	24.862412470682795
36-37	25.305522347866315	24.61811237424404	25.109443690477406	24.966921587412237
38-39	25.254965540993545	24.719079764403812	25.05662400601512	24.969330688587515
40-41	25.334081099939816	24.703262829041847	25.058377564870543	24.904278506147797
42-43	25.290796022980356	24.682584746043325	24.83489310413633	25.191726126839985
44-45	25.36865993119917	24.547755487516508	24.85316830077696	25.230416280507367
46-47	25.5257892001441	24.651746739189413	24.8694531533247	24.95301090734178
48-49	25.387769469892547	24.746570164044815	24.76145801335071	25.10420235271193
50-51	25.057750249762904	24.944372788891226	24.958082580878056	25.03979438046781
52-53	25.34999722337325	24.907335934311135	24.6715783549304	25.071088487385218
54-55	25.29638317693618	24.570076441895534	24.881123426997853	25.25241695417043
56-57	25.328090601228098	24.668425887726727	24.83249637901982	25.170987132025356
58-59	25.518608079193754	24.591130998618052	24.773565334525912	25.11669558766228
60-61	25.382401186474063	24.58374921169326	24.982560636198357	25.05128896563432
62-63	25.17011625579485	24.76888797687739	24.928472395213706	25.132523372114058
64-65	25.24525555148655	24.724505268513912	24.836225738556248	25.194013441443293
66-67	25.372843392849493	24.700515184235343	24.701789733255612	25.224851689659555
68-69	25.23645233508579	24.640590075512016	24.78954844295825	25.33340914644394
70-71	25.277558907419312	24.544219114083287	24.855795546813688	25.32242643168371
72-73	25.394374802880026	24.525130121657863	24.87165129805187	25.208843777410237
74-75	25.111899016724	24.890592701817514	24.753904141825885	25.2436041396326
76-77	25.389801191377348	24.516345028346006	24.711899209404436	25.381954570872207
78-79	25.307898300140756	24.550511488167206	24.84867941668975	25.29291079500229
80-81	25.347228439863372	24.548146188867044	24.790413055370795	25.314212315898793
82-83	25.412081025452533	24.51031088192845	24.801919360807677	25.275688731811336
84-85	25.306461663332442	24.673591957481854	24.687146003900402	25.332800375285302
86-87	25.27999224557761	24.648212474049757	24.833890047266454	25.23790523310618
88-89	25.253645555855968	24.454855985628015	24.67451106982074	25.61698738869528
90-91	25.24418018468938	24.410769543871456	24.747053506128147	25.597996765311017
92-93	25.415283980743276	24.63721415911614	24.74838753658708	25.199114323553506
94-95	25.466832183496706	24.443022226466983	24.75621783063877	25.33392775939754
96-97	25.33000840891565	24.429866817245202	24.850621830431013	25.389502943408132
98	25.432622769110758	24.571181282398257	24.51214297826024	25.484052970230742
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	849.0
1	630.5
2	578.5
3	774.0
4	687.5
5	649.5
6	1043.0
7	1996.5
8	2954.5
9	3084.5
10	2517.5
11	2152.0
12	2351.0
13	2845.5
14	3411.0
15	3919.0
16	4382.5
17	4617.5
18	4648.0
19	4539.0
20	4207.0
21	3748.0
22	3379.0
23	3345.5
24	3457.5
25	3699.5
26	4327.0
27	5321.5
28	6665.0
29	8694.0
30	11692.5
31	16044.0
32	22580.5
33	31393.0
34	43051.0
35	57243.0
36	73608.5
37	92579.0
38	113152.5
39	133409.0
40	153668.0
41	175255.0
42	198360.5
43	222439.5
44	244662.5
45	267591.0
46	298186.0
47	327564.0
48	339034.0
49	335414.0
50	331837.0
51	333562.5
52	336878.0
53	338993.5
54	335452.0
55	325363.5
56	307939.5
57	287277.5
58	264734.0
59	237951.0
60	209240.0
61	176444.0
62	141926.0
63	108312.0
64	79786.0
65	60327.5
66	46613.0
67	35843.5
68	27292.0
69	20679.5
70	15378.5
71	11303.0
72	8633.0
73	6586.5
74	4968.0
75	3667.5
76	2592.5
77	1658.0
78	1010.0
79	613.0
80	361.5
81	216.5
82	123.0
83	73.5
84	45.0
85	27.5
86	23.5
87	26.0
88	21.5
89	18.0
90	14.5
91	11.0
92	8.0
93	6.5
94	7.5
95	7.5
96	6.0
97	4.0
98	2.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4745986079338122
2	0.048095598987801705
3	0.05721643749784329
4	0.05501139961629477
5	0.06522043863930835
6	0.06617977330205999
7	0.043986210208552194
8	0.01990261464514569
9	0.012184982059725884
10-11	0.014805254347540028
12-13	0.011161214471864075
14-15	0.01544958359864187
16-17	0.014719343780726452
18-19	0.055791753931518105
20-21	0.08477941101719971
22-23	0.10158208604315545
24-25	0.07094065054631246
26-27	0.0869056975458358
28-29	0.08864538652381075
30-31	0.05866975791977299
32-33	0.03339057363487752
34-35	0.4006367691212222
36-37	0.6254289264028515
38-39	0.5752643145976228
40-41	0.5694796697655085
42-43	0.5426827321335732
44-45	0.5276698605829003
46-47	0.5171529753621381
48-49	0.5066933638525849
50-51	0.521448503702817
52-53	0.603242522523245
54-55	0.573309849202614
56-57	0.5296815996891183
58-59	0.5080106592103931
60-61	0.5206538309597915
62-63	0.5328388130195173
64-65	0.5519395957077362
66-67	0.5780993633024709
68-69	0.5312065122500593
70-71	0.4803188198408163
72-73	0.46305079591128706
74-75	0.4435061419611979
76-77	0.4577529776244497
78-79	0.45173207873359805
80-81	0.448789641820233
82-83	0.46870657489318096
84-85	0.4347647417879163
86-87	0.43750672071204966
88-89	0.464296499130084
90-91	0.44747950567632594
92-93	0.44248953358723725
94-95	0.4530708517331097
96-97	0.44770144130726097
98	0.4423535085231157
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	6984007.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.09591028526579
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.34212169944007	33.12826252832078
2	14.296357704815096	13.465999597440803
3	5.6799547469812035	8.02507917564589
4	2.9860052112523934	5.625145341619153
5	1.7729380371739538	4.174906537003987
6	1.0948265366865544	3.093711140983093
7	0.7606491910826303	2.507642624325129
8	0.5158912655607394	1.943709500784065
9	0.3767117574723817	1.5967424819991682
>10	1.9953041318965474	16.578708401103633
>50	0.12204153144123647	3.9131358941038994
>100	0.05459793581446252	4.350877409743988
>500	0.001835512946782006	0.5807161398543046
>1k	6.729689444051134E-4	0.6909886760970196
>5k	6.117899439716765E-5	0.17032689270645465
>10k+	3.0589497198583826E-5	0.15404765826863906
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	10693	0.15310694848959916	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4318427802263084E-5
2	0.0	0.0	0.0	0.0	1.4318427802263084E-5
3	0.0	0.0	0.0	0.0	1.4318427802263084E-5
4	0.0	0.0	0.0	2.8636855604526168E-5	4.295528340678925E-5
5	0.0	0.0	0.0	2.8636855604526168E-5	5.7273711209052336E-5
6	1.4318427802263084E-5	0.0	0.0	2.8636855604526168E-5	1.1454742241810467E-4
7	1.4318427802263084E-5	4.295528340678925E-5	0.0	2.8636855604526168E-5	1.2886585022036775E-4
8	1.4318427802263084E-5	4.295528340678925E-5	0.0	4.295528340678925E-5	1.2886585022036775E-4
9	1.4318427802263084E-5	4.295528340678925E-5	0.0	4.295528340678925E-5	1.5750270582489393E-4
10-11	2.8636855604526168E-5	4.295528340678925E-5	0.0	5.7273711209052336E-5	1.5750270582489393E-4
12-13	5.7273711209052336E-5	5.7273711209052336E-5	0.0	8.59105668135785E-5	3.4364226725431407E-4
14-15	5.7273711209052336E-5	8.591056681357852E-5	0.0	8.59105668135785E-5	4.1523440626562943E-4
16-17	5.7273711209052336E-5	1.002289946158416E-4	0.0	1.002289946158416E-4	4.5103047577128716E-4
18-19	5.7273711209052336E-5	1.002289946158416E-4	0.0	1.2170663631923621E-4	5.15463400881471E-4
20-21	5.7273711209052336E-5	1.002289946158416E-4	0.0	1.360250641214993E-4	6.156923954973126E-4
22-23	5.7273711209052336E-5	1.002289946158416E-4	0.0	1.9329877533055166E-4	7.230806040142858E-4
24-25	7.875135291244697E-5	1.0738820851697313E-4	0.0	2.8636855604526165E-4	8.161503847289958E-4
26-27	9.306978071471004E-5	1.1454742241810467E-4	1.4318427802263084E-5	3.364830533531825E-4	8.877425237403113E-4
28-29	1.002289946158416E-4	1.1454742241810467E-4	1.4318427802263084E-5	6.443292511018389E-4	9.306978071471005E-4
30-31	1.002289946158416E-4	1.2886585022036775E-4	1.4318427802263084E-5	0.0012170663631923622	9.521754488504952E-4
32-33	1.1454742241810467E-4	1.2886585022036775E-4	1.4318427802263084E-5	0.0026990236407265916	9.951307322572844E-4
34-35	1.1454742241810467E-4	1.2886585022036775E-4	1.4318427802263084E-5	0.004768036458153607	0.0011025189407742576
36-37	1.1454742241810467E-4	1.4318427802263083E-4	1.4318427802263084E-5	0.007323875820857568	0.0011025189407742576
38-39	1.1454742241810467E-4	1.5750270582489393E-4	1.4318427802263084E-5	0.011676677872745546	0.001123996582477652
40-41	1.2886585022036775E-4	1.5750270582489393E-4	1.4318427802263084E-5	0.016816993453757992	0.0011311557963787837
42-43	1.2886585022036775E-4	1.5750270582489393E-4	1.4318427802263084E-5	0.02177832868724215	0.0011526334380821783
44-45	1.360250641214993E-4	1.5750270582489393E-4	1.4318427802263084E-5	0.028099914561941303	0.001202747935390099
46-47	1.4318427802263085E-4	1.5750270582489393E-4	1.4318427802263084E-5	0.03482241641510382	0.001295817716104809
48-49	1.4318427802263085E-4	1.6466191972602546E-4	1.4318427802263084E-5	0.04173821704359689	0.0014246835663251768
50-51	1.4318427802263085E-4	1.7898034752828856E-4	2.1477641703394626E-5	0.048381967543846965	0.0014747980636330977
52-53	1.5750270582489393E-4	1.861395614294201E-4	2.8636855604526168E-5	0.05511878782481175	0.0015893454860512024
54-55	1.5750270582489393E-4	3.1500541164978786E-4	2.8636855604526168E-5	0.0626645992766044	0.0016537784111613865
56-57	1.5750270582489393E-4	3.722791228588402E-4	3.579606950565771E-5	0.07167089036422787	0.001675256052864781
58-59	1.5750270582489393E-4	3.865975506611033E-4	4.295528340678925E-5	0.08074161437696153	0.0017038929084693072
60-61	1.861395614294201E-4	3.865975506611033E-4	4.295528340678925E-5	0.08914653149688997	0.0017611666196783594
62-63	1.861395614294201E-4	3.865975506611033E-4	4.295528340678925E-5	0.09813850415671119	0.0018184403308874118
64-65	1.861395614294201E-4	4.009159784633664E-4	4.295528340678925E-5	0.1074025269447754	0.0018184403308874118
66-67	1.861395614294201E-4	4.152344062656295E-4	4.295528340678925E-5	0.116960077502786	0.0018399179725908064
68-69	1.861395614294201E-4	4.152344062656295E-4	4.295528340678925E-5	0.1274912811513505	0.0019043508977009903
70-71	1.861395614294201E-4	4.7250811747468185E-4	4.295528340678925E-5	0.1386095403398078	0.0019186693255032533
72-73	1.861395614294201E-4	5.297818286837342E-4	4.295528340678925E-5	0.1491192663466689	0.0022909484483620932
74-75	1.861395614294201E-4	5.441002564859972E-4	4.295528340678925E-5	0.16002274911809222	0.002348222159571146
76-77	1.861395614294201E-4	5.441002564859972E-4	4.295528340678925E-5	0.17165647170743098	0.0023696998012745405
78-79	1.861395614294201E-4	5.441002564859972E-4	4.295528340678925E-5	0.18526613733348207	0.0024054958707801985
80-81	1.861395614294201E-4	5.512594703871287E-4	4.295528340678925E-5	0.19908342016266592	0.0024484511541869878
82-83	1.861395614294201E-4	5.584186842882603E-4	4.295528340678925E-5	0.2121203486766265	0.00250572486539604
84-85	1.861395614294201E-4	5.584186842882603E-4	5.7273711209052336E-5	0.2255152378856436	0.00250572486539604
86	1.861395614294201E-4	5.727371120905234E-4	5.7273711209052336E-5	0.23635428773195674	0.00250572486539604
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	27780	0.0	24.40895	1
TACATGG	28395	0.0	23.394897	2
ACATGGG	28975	0.0	21.977255	3
GTATCAA	26800	0.0	21.522608	1
CATGGGG	22740	0.0	18.971022	4
GAGTACA	20560	0.0	18.76288	1
TATCAAC	30805	0.0	18.528608	2
TCAACGC	31010	0.0	18.317286	4
CAACGCA	31110	0.0	18.243668	5
ATCAACG	31460	0.0	18.08455	3
AGAGTAC	22335	0.0	17.987175	10-11
AGTACAT	20015	0.0	17.957884	2
AACGCAG	32215	0.0	17.656288	6
ACGCAGA	35695	0.0	15.85289	7
ATACCGT	1045	0.0	15.789629	6
CGCAGAG	37150	0.0	15.228184	8
GTACCTG	6570	0.0	15.20453	1
GTGTAGC	3510	0.0	15.082361	1
ATGGGGG	10660	0.0	14.837251	5
GGTATCA	20730	0.0	14.678469	1
>>END_MODULE