FastQCFastQC Report
Mon 15 Aug 2016
SRR2921422_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921422_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6984007
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT114280.16363099292426253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG279600.024.6173951
TACATGG288450.023.4139212
ACATGGG291450.022.2608183
GTATCAA283550.021.2170561
CATGGGG230600.019.5670344
GAGTACA211750.018.7159251
AGTACAT204200.018.3047642
AGAGTAC234900.018.27100410-11
TATCAAC326550.018.1950282
TCAACGC325450.018.067464
CAACGCA326350.017.9513055
ATCAACG335750.017.5270863
AACGCAG341750.017.2263496
GTATATA12850.016.3102631
ACGCAGA379550.015.4236317
GTATAGG13100.015.2717711
GTACCTG67000.015.2142441
GTATTAG10700.015.1358411
TATAACG4101.07265805E-815.0592952
ATGGGGG110300.014.9784985
Produced by FastQC (version 0.11.5)