##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921422_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6984007
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4743517582385	33.0	31.0	34.0	30.0	34.0
2	31.606590886864804	33.0	31.0	34.0	28.0	34.0
3	31.729432115403092	34.0	31.0	34.0	28.0	34.0
4	35.3256170848626	37.0	35.0	37.0	33.0	37.0
5	35.055683363433054	37.0	35.0	37.0	32.0	37.0
6	35.08518032699566	37.0	35.0	37.0	32.0	37.0
7	34.996521910702555	37.0	35.0	37.0	32.0	37.0
8	34.94063765972743	37.0	35.0	37.0	32.0	37.0
9	36.48373491034588	39.0	37.0	39.0	32.0	39.0
10-11	36.360297892026736	39.0	37.0	39.0	32.0	39.0
12-13	36.22087692638338	39.0	37.0	39.0	32.0	39.0
14-15	37.38939644533575	40.0	38.0	41.0	32.0	41.0
16-17	37.2581717057271	40.0	37.0	41.0	32.0	41.0
18-19	37.13134036091316	40.0	37.0	41.0	31.0	41.0
20-21	36.993155505141964	40.0	37.0	41.0	31.0	41.0
22-23	36.94412548555579	40.0	37.0	41.0	31.0	41.0
24-25	36.983021351496355	40.0	37.0	41.0	31.0	41.0
26-27	36.84474213728595	40.0	37.0	41.0	30.5	41.0
28-29	36.68247318480637	40.0	36.5	41.0	30.0	41.0
30-31	36.52052181505546	39.0	36.0	41.0	30.0	41.0
32-33	36.3302271890621	39.0	36.0	41.0	30.0	41.0
34-35	36.15059470873955	39.0	36.0	40.0	30.0	41.0
36-37	36.02498365193506	39.0	35.0	40.0	29.0	41.0
38-39	35.9659303749266	39.0	35.0	40.0	29.0	41.0
40-41	35.77343608046212	39.0	35.0	40.0	28.5	41.0
42-43	35.48601010852366	38.5	35.0	40.0	27.0	41.0
44-45	35.239830730410205	38.0	35.0	40.0	27.0	41.0
46-47	34.92212514964547	38.0	34.0	40.0	26.0	41.0
48-49	34.53065260387053	38.0	34.0	40.0	24.0	41.0
50-51	34.196518273821894	38.0	33.0	40.0	24.0	41.0
52-53	33.825069834552	37.5	33.0	40.0	23.0	41.0
54-55	33.42962879332738	37.0	32.5	40.0	22.0	41.0
56-57	32.971486640835266	36.5	32.0	39.0	20.5	40.0
58-59	32.50889467894291	36.0	31.0	39.0	18.5	40.0
60-61	32.00378665141659	35.5	31.0	39.0	16.0	40.0
62-63	31.504609459870245	35.0	30.5	38.0	12.5	40.0
64-65	30.913853322311965	34.5	29.5	38.0	8.0	40.0
66-67	30.309896238649245	34.0	29.0	37.0	7.0	39.0
68-69	29.740649744480496	34.0	28.5	36.5	4.5	39.0
70-71	29.11668473413615	33.5	27.5	36.0	2.0	39.0
72-73	28.500846290675256	33.0	26.0	35.0	2.0	38.0
74-75	27.92747859502432	32.5	26.0	35.0	2.0	37.0
76-77	26.861054764120368	31.0	25.5	34.0	2.0	35.5
78-79	27.805173305238668	32.5	26.5	35.0	2.0	36.0
80-81	27.807761647432486	33.0	26.5	35.0	2.0	36.0
82-83	27.410300777189942	33.0	26.0	35.0	2.0	35.5
84-85	26.833634187365504	32.0	25.0	34.5	2.0	35.0
86-87	26.22024025749115	32.0	24.5	34.0	2.0	35.0
88-89	25.589839972955353	31.5	22.5	34.0	2.0	35.0
90-91	24.97937144106528	31.0	19.5	34.0	2.0	35.0
92-93	24.408780661302316	31.0	18.0	34.0	2.0	35.0
94-95	23.7393300865821	31.0	9.5	34.0	2.0	35.0
96-97	22.758319185533463	30.0	2.0	34.0	2.0	35.0
98-99	21.51659806469266	29.5	2.0	34.0	2.0	35.0
100-101	18.031537840669404	23.5	2.0	31.5	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77608.0
3	36849.0
4	21758.0
5	15935.0
6	13828.0
7	14218.0
8	16520.0
9	19596.0
10	22895.0
11	27005.0
12	31086.0
13	36032.0
14	39801.0
15	44318.0
16	49247.0
17	54884.0
18	56849.0
19	54414.0
20	56335.0
21	59240.0
22	63585.0
23	70176.0
24	78490.0
25	91185.0
26	108030.0
27	129728.0
28	156896.0
29	189454.0
30	232098.0
31	286233.0
32	362311.0
33	475364.0
34	645561.0
35	895482.0
36	1165740.0
37	1019201.0
38	262626.0
39	3429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.963127875360705	18.43781464687016	14.922775513956138	23.676281963813008
2	16.28188516993182	19.760948120470097	37.2644958689188	26.692670840679284
3	19.947757431471008	23.30192679195776	28.017203791123084	28.733111985448147
4	14.636247146092554	15.961795463089464	34.43982897185979	34.9621284189582
5	16.280726050604986	35.82102802186033	32.05271610326267	15.84552982427202
6	35.03481794277057	33.07023291192851	16.519692916103537	15.375256229197385
7	31.2148215250104	30.336313421199428	19.442540683837812	19.006324369952356
8	28.641720179324377	32.27775728143372	18.918670220130736	20.161852319111166
9	27.292031639096916	16.167041384434057	18.92945751720912	37.61146945925991
10-11	26.889423123088736	24.741840813909977	25.746565939282952	22.62217012371834
12-13	27.23924708008884	21.919969994534494	26.248648224111705	24.592134701264964
14-15	24.50787081459338	23.318548169293614	23.97051286772054	28.20306814839246
16-17	24.727196838386714	26.245724592956325	24.54454981659383	24.482528752063132
18-19	24.78483503750969	25.772741424108403	25.729089280200856	23.713334258181053
20-21	25.67415256006354	24.648607531591573	25.439593723528077	24.237646184816807
22-23	25.29464916627024	24.387707436894964	25.42139138837596	24.89625200845884
24-25	25.167459187339798	24.717134323928672	25.336051706575823	24.779354782155703
26-27	25.05746848238094	24.666599737659293	25.573163167525546	24.702768612434216
28-29	24.91868238794038	25.071904583325065	25.334660203984523	24.67475282475003
30-31	25.184978939735686	24.768119444975838	25.175378105343704	24.871523509944772
32-33	25.027129579837144	24.744016833119417	25.185543272118366	25.043310314925076
34-35	25.29951407161789	24.720780377970073	25.259097007612226	24.72060854279981
36-37	25.225509302726373	24.687077940875223	25.286569710357583	24.80084304604082
38-39	25.202073073106046	24.774433721378173	25.216723209797692	24.806769995718092
40-41	25.30577186782813	24.716848727009737	25.19726605156407	24.78011335359806
42-43	25.207960802821628	24.82608958373188	24.95594784935582	25.010001764090674
44-45	25.277374858210422	24.5879706873449	25.014975528632938	25.11967892581174
46-47	25.407270342034334	24.7046978520095	25.076056164552284	24.811975641403887
48-49	25.315206969705994	24.843796178139655	24.919041487872544	24.921955364281803
50-51	25.036401805480867	24.967289030084928	25.06309324095958	24.933215923474624
52-53	25.332555251305532	24.93462228048053	24.76999936782625	24.962823100387688
54-55	25.354690905128447	24.613796526679938	24.89035394667442	25.141158621517196
56-57	25.349813177379215	24.749721264186565	24.8621892765985	25.03827628183572
58-59	25.600720282288115	24.65933034680982	24.773485295561088	24.966464075340976
60-61	25.45629330613261	24.670828823685532	25.007129163400997	24.865748706780856
62-63	25.21154310470009	24.794951262885988	24.968595042742532	25.02491058967139
64-65	25.294227316609643	24.765312636302532	24.868011179420886	25.072448867666942
66-67	25.44649465945326	24.745663610120356	24.67517585591736	25.132665874509026
68-69	25.302346925340263	24.689486666763134	24.819182118489422	25.18898428940718
70-71	25.36912768977314	24.573631249859933	24.88839443139604	25.168846628970886
72-73	25.51411933985329	24.557036667400954	24.850569520192522	25.07827447255323
74-75	25.213045808340645	24.920253315609777	24.69693251568897	25.169768360360607
76-77	25.40651739049603	24.575864372535484	24.792159019255543	25.225459217712938
78-79	25.323685011617403	24.62878289248619	24.927867205590626	25.11966489030578
80-81	25.380664964127803	24.63590685976112	24.831846834523823	25.15158134158726
82-83	25.393733942927987	24.620001128596076	24.880358790431455	25.10590613804449
84-85	25.307881054696914	24.75696860886749	24.78026192919819	25.154888407237415
86-87	25.265690226920245	24.691225646286988	24.86972624413211	25.173357882660657
88-89	25.26091141508806	24.565099664098074	24.73966071566721	25.434328205146656
90-91	25.280025084031006	24.56019253456169	24.77911866247749	25.380663718929817
92-93	25.491000816320213	24.787856661991643	24.77518604286537	24.94595647882277
94-95	25.449574580890488	24.61540444260198	24.73150106162357	25.20351991488396
96-97	25.26728569443304	24.566091444001078	24.95505353231517	25.21156932925071
98-99	25.35784297475157	24.610320200485003	24.6962828493346	25.33555397542883
100-101	26.006939693628482	23.810496517516164	24.848994755385572	25.33356903346978
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	271.0
1	236.5
2	360.5
3	537.5
4	409.5
5	316.5
6	669.5
7	1627.5
8	2517.0
9	2493.5
10	1781.5
11	1173.5
12	1065.0
13	1132.5
14	1223.5
15	1341.0
16	1437.5
17	1568.5
18	1753.5
19	1884.5
20	2006.0
21	2118.5
22	2264.0
23	2518.5
24	2809.0
25	3247.5
26	3773.5
27	4452.5
28	5645.5
29	7445.0
30	10161.0
31	14064.5
32	19721.5
33	27239.5
34	36664.5
35	48739.0
36	62915.0
37	79533.0
38	98832.0
39	117380.0
40	136990.5
41	157770.0
42	178544.5
43	201886.0
44	225858.5
45	248081.5
46	269283.0
47	296411.0
48	330276.0
49	341984.5
50	329636.0
51	326469.0
52	331516.5
53	333070.0
54	331576.0
55	322224.0
56	305123.0
57	282976.0
58	258994.0
59	234035.0
60	205343.0
61	175497.0
62	142827.0
63	108860.5
64	80912.0
65	61281.0
66	46858.5
67	36038.5
68	27698.0
69	21029.5
70	16121.5
71	12211.0
72	8966.0
73	6785.0
74	5084.0
75	3734.5
76	2672.5
77	1703.5
78	1069.0
79	623.0
80	359.5
81	205.0
82	102.5
83	49.5
84	29.5
85	17.5
86	11.5
87	8.0
88	3.0
89	1.0
90	2.0
91	2.0
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31267150791801895
2	0.0
3	0.0061569239549731265
4	0.05874850927268544
5	4.438712618701556E-4
6	0.06323017717479379
7	0.07345353462560962
8	0.10241971406958784
9	0.09036359786008233
10-11	0.0948739026177952
12-13	0.09466628541466239
14-15	0.06426110397655672
16-17	0.03591061692807582
18-19	0.038337590440559406
20-21	0.028114232989743568
22-23	0.04163798804898105
24-25	0.03408501738328727
26-27	0.02124854685855842
28-29	0.023818704649064642
30-31	0.003422104244740877
32-33	0.005634301340190524
34-35	0.00816866306119109
36-37	0.010860527488016549
38-39	0.016280052411173127
40-41	0.020503988612840737
42-43	0.003422104244740877
44-45	0.013573869556545406
46-47	0.023868819146372563
48-49	0.0026202722878141444
50-51	0.007066144120416833
52-53	0.002398336656879067
54-55	0.00408791113754611
56-57	0.0034865371698510612
58-59	0.0011239965824776522
60-61	0.005276340645133946
62-63	0.014790935919737767
64-65	0.0066007952168432825
66-67	0.037979629745502834
68-69	0.06005148620269137
70-71	0.011748270011756861
72-73	0.030999396191899578
74-75	0.04962767076264385
76-77	0.30142438287934137
78-79	0.07214339848170255
80-81	0.03260306010575305
82-83	0.026932962696056866
84-85	0.018878847057283876
86-87	0.11626563375437625
88-89	0.5196801778692375
90-91	0.1296032492521843
92-93	0.047114786683346684
94-95	0.00858389746745672
96-97	0.0060352173186538904
98-99	0.010617114215378077
100-101	0.00798252349976167
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	6984007.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.389404291264945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.61491736242264	30.013859029210483
2	15.506753601991438	13.766711097676538
3	6.307495031005573	8.399578409893534
4	3.215980963897784	5.710219167978827
5	1.9222032762991528	4.2662729180818575
6	1.177662532245866	3.136544295752196
7	0.7941062361413105	2.467493193640394
8	0.5767069824498217	2.047974352124846
9	0.44043335159110375	1.7595516696420912
>10	2.2376780458292633	17.685663389186256
>50	0.1402041807937316	4.267548352592659
>100	0.06304516636294295	4.846042089444609
>500	0.0019402291134203108	0.5741205575405117
>1k	7.760354282271428E-4	0.7312109113047363
>5k	6.466961845034504E-5	0.16318186270398324
>10k+	3.233480922517252E-5	0.16402870322641572
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	11428	0.16363099292426253	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8636855604526168E-5
2	0.0	0.0	0.0	0.0	2.8636855604526168E-5
3	0.0	1.4318427802263084E-5	0.0	0.0	2.8636855604526168E-5
4	0.0	1.4318427802263084E-5	0.0	0.0	2.8636855604526168E-5
5	0.0	5.7273711209052336E-5	0.0	0.0	4.295528340678925E-5
6	0.0	5.7273711209052336E-5	0.0	0.0	5.7273711209052336E-5
7	0.0	5.7273711209052336E-5	0.0	2.8636855604526168E-5	5.7273711209052336E-5
8	0.0	7.159213901131543E-5	0.0	2.8636855604526168E-5	7.159213901131543E-5
9	0.0	7.159213901131543E-5	0.0	4.295528340678925E-5	7.159213901131543E-5
10-11	7.159213901131542E-6	7.159213901131543E-5	0.0	4.295528340678925E-5	7.875135291244697E-5
12-13	1.4318427802263084E-5	7.159213901131543E-5	0.0	6.443292511018389E-5	3.5080148115544554E-4
14-15	1.4318427802263084E-5	8.59105668135785E-5	0.0	7.159213901131543E-5	4.868265452769449E-4
16-17	1.4318427802263084E-5	8.59105668135785E-5	0.0	7.875135291244697E-5	5.584186842882603E-4
18-19	1.4318427802263084E-5	8.59105668135785E-5	0.0	1.0738820851697313E-4	6.443292511018388E-4
20-21	1.4318427802263084E-5	1.1454742241810467E-4	0.0	1.1454742241810467E-4	7.946727430256012E-4
22-23	1.4318427802263084E-5	1.1454742241810467E-4	0.0	1.503434919237624E-4	8.877425237403113E-4
24-25	2.8636855604526168E-5	1.2886585022036778E-4	0.0	2.4341327263847242E-4	9.163793793448374E-4
26-27	4.295528340678925E-5	1.4318427802263085E-4	0.0	2.7920934214413013E-4	9.87971518356153E-4
28-29	4.295528340678925E-5	1.503434919237624E-4	0.0	6.085331815961811E-4	0.001016608373960679
30-31	5.7273711209052336E-5	1.71821133627157E-4	0.0	0.0011597926519833098	0.0010309268017629422
32-33	1.002289946158416E-4	1.71821133627157E-4	0.0	0.0025773170044073555	0.0010738820851697313
34-35	1.0738820851697313E-4	1.9329877533055166E-4	0.0	0.004581896896724187	0.0011168373685765205
36-37	1.1454742241810467E-4	2.004579892316832E-4	0.0	0.0070231888370100425	0.0011311557963787837
38-39	1.1454742241810467E-4	2.004579892316832E-4	0.0	0.011189851327468602	0.0011454742241810466
40-41	1.2886585022036775E-4	2.004579892316832E-4	0.0	0.016308689266777653	0.0011597926519833098
42-43	1.2886585022036775E-4	2.004579892316832E-4	0.0	0.02106956651103013	0.0011669518658844412
44-45	1.2886585022036775E-4	2.2909484483620934E-4	0.0	0.02716205754089307	0.0012027479353900992
46-47	1.360250641214993E-4	2.2909484483620934E-4	0.0	0.033791489613340886	0.0012600216465991514
48-49	1.4318427802263085E-4	2.362540587373409E-4	0.0	0.04032785190507398	0.0013888874968195192
50-51	1.4318427802263085E-4	2.50572486539604E-4	0.0	0.046592164068564076	0.0014246835663251768
52-53	1.503434919237624E-4	2.64890914341867E-4	0.0	0.05294954601276889	0.0015392309887432816
54-55	1.71821133627157E-4	3.7227912285884017E-4	0.0	0.06015171519730722	0.0015821862721500709
56-57	1.71821133627157E-4	3.865975506611033E-4	0.0	0.06865686131185149	0.001610823127754597
58-59	1.71821133627157E-4	3.937567645622348E-4	0.0	0.07729087327661613	0.0016537784111613863
60-61	1.71821133627157E-4	4.009159784633664E-4	0.0	0.08520896385126761	0.0017325297640738333
62-63	1.71821133627157E-4	4.1523440626562943E-4	0.0	0.0934921743348768	0.0019043508977009903
64-65	1.7898034752828856E-4	4.2955283406789253E-4	0.0	0.10221925608035617	0.0019043508977009903
66-67	1.861395614294201E-4	4.2955283406789253E-4	0.0	0.11097497468144005	0.0019115101116021218
68-69	1.861395614294201E-4	4.2955283406789253E-4	0.0	0.12066139108967101	0.0019473061811077796
70-71	2.004579892316832E-4	4.2955283406789253E-4	0.0	0.13088474854048684	0.002004579892316832
72-73	2.004579892316832E-4	4.2955283406789253E-4	0.0	0.1407859413657518	0.002247993164955304
74-75	2.004579892316832E-4	4.2955283406789253E-4	0.0	0.15119543837799704	0.002348222159571146
76-77	2.1477641703394627E-4	4.2955283406789253E-4	0.0	0.1622635830691464	0.002398336656879067
78-79	2.2909484483620934E-4	4.2955283406789253E-4	0.0	0.1749282324602481	0.002441291940285856
80-81	2.2909484483620934E-4	4.2955283406789253E-4	0.0	0.18782913591008715	0.002512884079297171
82-83	2.4341327263847242E-4	4.2955283406789253E-4	0.0	0.20019309831734133	0.00269186442682546
84-85	2.577317004407355E-4	4.2955283406789253E-4	0.0	0.2130080912003668	0.0026990236407265916
86-87	2.577317004407355E-4	4.2955283406789253E-4	0.0	0.22693276223806763	0.0027777749936390385
88-89	2.577317004407355E-4	4.2955283406789253E-4	0.0	0.2416377875909918	0.0028422079187492223
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	27960	0.0	24.617395	1
TACATGG	28845	0.0	23.413921	2
ACATGGG	29145	0.0	22.260818	3
GTATCAA	28355	0.0	21.217056	1
CATGGGG	23060	0.0	19.567034	4
GAGTACA	21175	0.0	18.715925	1
AGTACAT	20420	0.0	18.304764	2
AGAGTAC	23490	0.0	18.271004	10-11
TATCAAC	32655	0.0	18.195028	2
TCAACGC	32545	0.0	18.06746	4
CAACGCA	32635	0.0	17.951305	5
ATCAACG	33575	0.0	17.527086	3
AACGCAG	34175	0.0	17.226349	6
GTATATA	1285	0.0	16.310263	1
ACGCAGA	37955	0.0	15.423631	7
GTATAGG	1310	0.0	15.271771	1
GTACCTG	6700	0.0	15.214244	1
GTATTAG	1070	0.0	15.135841	1
TATAACG	410	1.07265805E-8	15.059295	2
ATGGGGG	11030	0.0	14.978498	5
>>END_MODULE