FastQCFastQC Report
Mon 15 Aug 2016
SRR2921420_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921420_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3214210
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26350.048.088181
GTATCAA45750.042.6342281
TCAACGC53500.035.3067134
CAACGCA54800.034.466465
ATCAACG56950.033.2511863
TATCAAC57650.032.8479542
AACGCAG59700.031.9415956
GTACATG102550.028.5996341
TACATGG104600.027.6778832
ACGCAGA72900.025.7739337
ACATGGG110550.025.4150493
CGCAGAG74200.025.0061458
CATGGGG96600.023.8282764
GAGTACA75950.022.1823861
GCAGAGT87000.020.0189
AGTACAT78150.019.6159022
ATGGGGG61250.019.4474125
AGAGTAC68650.017.5922410-11
TACACTG36750.017.3038065
CAGAGTA77750.017.27268810-11
Produced by FastQC (version 0.11.5)